Article
Gastroenterology & Hepatology
Chiaki Matsuzaki, Yukari Nakashima, Ikuto Endo, Yusuke Tomabechi, Yasuki Higashimura, Saki Itonori, Koji Hosomi, Jun Kunisawa, Kenji Yamamoto, Keiko Hisa
Summary: Leuconostoc mesenteroides strain NTM048 produces an exopolysaccharide with adjuvanticity for mucosal vaccination, and the key polymer structure for adjuvanticity was determined to be Gtf2-S-glucan. Gtf2-S-glucan, produced in the supernatant by Gtf2, showed a higher ability to stimulate IgA production and induced higher antigen-specific antibodies in the airway mucosa and plasma compared to native NTM048 EPS. These findings suggest a pivotal role of Gtf2-S-glucan in the adjuvanticity of NTM048 EPS.
Article
Biotechnology & Applied Microbiology
Seul-Gi Jeong, In Seong Choi, Ho Myeong Kim, Ji Yoon Chang, Hae Woong Park
Summary: Cold adaptation under supercooling conditions can improve the viability and storage stability of lactic acid bacteria during freeze-drying, with exopolysaccharides playing a protective role. Cold adaptation is a good strategy for long-term storage of Leuconostoc mesenteroides WiKim32.
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Qing Zhang, Jie Wang, Qing Sun, Shu-Ming Zhang, Xiang-Yang Sun, Chan-Yuan Li, Miao-Xin Zheng, Wen-Liang Xiang, Jie Tang
Summary: Strain LM187 and its rEPS414 demonstrate good tolerance and significant antioxidant activity, indicating promising prospects for industrial applications.
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Chemistry, Applied
Giulia Bisson, Clara Comuzzi, Elisa Giordani, Denise Poletti, Marta Boaro, Marilena Marino
Summary: This study characterized the chemical and biological properties of an exopolysaccharide EPS_B3 produced by Leuconostoc mesenteroides strain isolated from an Italian cheese. The EPS_B3 displayed antimicrobial, antibiofilm, bifidogenic, antioxidant, and DNA-protective activities. It showed potential applications in functional foods, especially for its antimicrobial activity and ability to improve the viability of bifidobacteria in probiotic foods. Further research is needed to assess its ability to reach the gastrointestinal tract intact.
CARBOHYDRATE POLYMERS
(2023)
Article
Immunology
Othman R. Alzahrani, Yousef M. Hawsawi, Abdullah D. Alanazi, Hanan E. Alatwi, Irfan A. Rather
Summary: This study is a preliminary investigation of the antiviral properties of postbiotic metabolites derived from Leuconostoc mesenteroides GBUT-21, focusing on their potential biological role in inactivating SARS-CoV-2 and reducing related inflammatory pathways.
Article
Food Science & Technology
Victoria A. Jideani, Mmaphuti A. Ratau, Vincent I. Okudoh
Summary: The study aimed to isolate and purify lactic acid bacteria from naturally fermented pearl millet slurry and evaluate their effectiveness as starter cultures for traditional non-alcoholic pearl millet fermented beverages. The findings suggest that appropriate LAB can have a positive impact on product quality and microbial stability, and adjustments in fermentation conditions can lead to reduced production time.
Article
Agriculture, Multidisciplinary
Hua Zhang, HePing HuangFu, GuangYong Qin, GuoFang Wu, Lei Wang, ZhongFang Tan
Summary: In this study, the antimicrobial effect of lactic acid on Gallibacterium anatis was investigated. The results showed that lactic acid caused damage to the cell membrane and lysis of G. anatis. Transcriptomic and metabolic analyses revealed changes in gene expression and metabolite levels in response to acid stress. Lactic acid disrupted energy metabolism in G. anatis. Overall, this study provides a comprehensive understanding of the stress response and cell death of G. anatis caused by lactic acid.
CHEMICAL AND BIOLOGICAL TECHNOLOGIES IN AGRICULTURE
(2023)
Article
Veterinary Sciences
Hua Zhang, HePing HuangFu, Xing Wang, ShanShan Zhao, Yuan Liu, Haoxin Lv, GuangYong Qin, Zhongfang Tan
Summary: This study demonstrated that Leuconostoc mesenteroides QZ1178 from Qula fermented food exhibited potential antibacterial effects against Gallibacterium anatis through the production of lactic acid, inhibiting the growth and causing cell damage of the poultry pathogen.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Microbiology
Junrui Wu, Danli Yan, Yumeng Liu, Xue Luo, Yang Li, Chengxu Cao, Mo Li, Qi Han, Cong Wang, Rina Wu, Lanwei Zhang
Summary: A novel EPS was extracted from Leuconostoc mesenteroides Shen Nong's (SN)-8, showing potential antioxidant and anticancer effects through structural characterization and experimental results.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Urology & Nephrology
Hongzhi Li, Zhichao Chen, Weitian Chen, Jingyi Li, Yunshuang Liu, Hongchuang Ma, Mingming Shi, Xuelian Sun, Xiusong Yao, Zhijun Li, Izabella Z. A. Pawluczyk, Shuchen Zhang, Jonathan Barratt, Jicheng Lv, Kai Wang, Binghai Zhao
Summary: The study revealed that miR-23b is downregulated in IgAN and may play a crucial role in kidney disease and the development of IgAN. It suggests that miR-23b could be a novel therapeutic target for treating IgAN in the future.
JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY
(2021)
Article
Food Science & Technology
Jing Lu, Yunren Mao, Ting Ma, Xiaoling Liu, Xiyao Cheng, Yunxia Bai, Shubo Li
Summary: Exopolysaccharide (EPS) produced by lactic acid bacteria (LAB) has various health-beneficial biological activities. This study identified five LAB strains with high EPS production, among which MY04 showed the best overall prebiotic properties and was determined safe for human consumption. MY04 is a promising probiotic candidate for the development of novel functional foods.
Article
Biochemistry & Molecular Biology
Tadao Oikawa, Kouhei Okajima, Kazuya Yamanaka, Shiro Kato
Summary: We successfully expressed selenocysteine beta-lyase (Lm-SCL) from Leuconostoc mesenteroides LK-151 in soluble fractions of Escherichia coli. Lm-SCL showed high preference for L-selenocysteine and had enough catalytic efficiency. It had optimal temperature and pH and remained stable under certain conditions.
Article
Biotechnology & Applied Microbiology
Nontobeko Cele, Babalwa Nyide, Thandeka Khoza
Summary: Unhealthy dietary patterns are associated with increased mortality rates and the prevalence of nontransmissible chronic degenerative diseases. This study focused on probiotic potential of lactic acid bacteria isolated from fermented carrot and ginger brine, particularly the strain Leuconostoc mesenteroides subsp. mesenteroides. The isolated strains exhibited high tolerance to gastrointestinal conditions, strong antibacterial activity, and antioxidant activity, suggesting great potential as a beneficial strain for functional food.
FERMENTATION-BASEL
(2022)
Article
Physiology
Mylissa A. Stover, Brenda Tinoco-Bravo, Crystal A. Shults, Sydney Marouk, Ratnakar Deole, Jacob R. Manjarrez
Summary: The study investigates the effects of Lactococcus lactis and Leuconostoc mesenteroides on stress and longevity in Caenorhabditis elegans and finds their potential in probiotic therapy. Fluorescent reporters and health span measurements are used to analyze the impact of these strains on the morphology, locomotor behavior, reproduction, and stress responses of the worms, revealing unique characteristics and stress response patterns for each group of microbes.
FRONTIERS IN PHYSIOLOGY
(2023)
Article
Chemistry, Medicinal
Shinsuke Inuki, Hitomi Tabuchi, Chiaki Matsuzaki, Yasunori Yonejima, Keiko Hisa, Ikuo Kimura, Kenji Yamamoto, Hiroaki Ohno
Summary: Exopolysaccharides (EPSs) are widely present in natural products made by bacteria, fungi, and algae, and some of them have interesting biological properties such as anticancer and immunomodulatory activities. Our recent study found that EPSs derived from Leuconostoc mesenteroides ssp. mesenteroides strain NTM048 enhanced the production of mucosal immunoglobulin A (IgA) in mice. In this study, we described the synthesis and evaluation of tetrasaccharide fragments of NTM048 EPS to gain insights into the molecular mechanism responsible for the induction of IgA.
CHEMICAL & PHARMACEUTICAL BULLETIN
(2022)
Review
Biochemistry & Molecular Biology
Tadasu Urashima, Takane Katayama, Mikiyasu Sakanaka, Kenji Fukuda, Michael Messer
Summary: This review discusses the acquisition of lactose and milk oligosaccharides during the evolution of mammals, as well as the mechanisms by which these saccharides are digested and absorbed by suckling neonates. The evolution of c-type lysozyme to alpha-lactalbumin enabled the biosynthesis of lactose and milk oligosaccharides in most eutherian mammals. In contrast, neonatal monotremes and marsupials lack intestinal lactase and may digest milk oligosaccharides through pinocytosis or endocytosis.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2022)
Article
Biotechnology & Applied Microbiology
Miriam N. Ojima, Yuya Asao, Aruto Nakajima, Toshihiko Katoh, Motomitsu Kitaoka, Aina Gotoh, Junko Hirose, Tadasu Urashima, Satoru Fukiya, Atsushi Yokota, Maher Abou Hachem, Mikiyasu Sakanaka, Takane Katayama
Summary: In this study, the authors examined the HMO consumption phenotypes of two bifidobacterial species and found that the diversity of the FL transporter led to uptake specificities of fucosylated sugars ranging from simple trisaccharides to complex hexasaccharides. These findings explain the adaptation and proliferation of bifidobacteria in the competitive and HMO-rich infant gut environment.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Daiki Oikawa, Satoshi Yamashita, Seiji Takahashi, Toshiyuki Waki, Koichi Kikuchi, Takaaki Abe, Takane Katayama, Toru Nakayama
Summary: The study found that sesamin and related lignans derived from sesame seeds can inhibit the activity of intestinal bacteria TILs, potentially serving as a mechanism-based strategy to prevent CKD progression. These phytochemicals competitively bind to EcTIL and may bind near the active site of the enzyme.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Review
Biochemical Research Methods
Miriam N. Ojima, Keisuke Yoshida, Mikiyasu Sakanaka, Lin Jiang, Toshitaka Odamaki, Takane Katayama
Summary: This article reviews clinical studies on proliferating human-residential Bifidobacterium strains in the gut and highlights the differences between responders and non-responders to such interventions from an ecological and genetic perspective. The criteria to better evaluate the efficacy of probiotic and prebiotic interventions and the therapeutic potential of synbiotics are discussed.
CURRENT OPINION IN BIOTECHNOLOGY
(2022)
Article
Oncology
Natsuki Nakagawa, Yoshiko Hashii, Hisako Kayama, Ryu Okumura, Hiroko Nakajima, Hikaru Minagawa, Soyoko Morimoto, Fumihiro Fujiki, Jun Nakata, Toshiro Shirakawa, Takane Katayama, Kiyoshi Takeda, Akihiro Tsuboi, Keiichi Ozono
Summary: Orally administered genetically engineered Bifidobacterium longum expressing WT1 protein showed antitumor activity by inducing WT1-specific immune responses and activating dendritic cells. This oral vaccine platform could potentially be a novel approach for cancer immunotherapy.
CANCER IMMUNOLOGY IMMUNOTHERAPY
(2023)
Article
Ecology
Miriam N. Ojima, Lin Jiang, Aleksandr A. Arzamasov, Keisuke Yoshida, Toshitaka Odamaki, Jinzhong Xiao, Aruto Nakajima, Motomitsu Kitaoka, Junko Hirose, Tadasu Urashima, Toshihiko Katoh, Aina Gotoh, Douwe van Sinderen, Dmitry A. Rodionov, Andrei L. Osterman, Mikiyasu Sakanaka, Takane Katayama
Summary: This study applied assembly theory to investigate the formation of bifidobacterial communities in the infant gut. The results showed that arrival order and sugar consumption phenotypes significantly affected community formation. Bifidobacterium bifidum and Bifidobacterium longum subsp. infantis dominated through inhibitory priority effects, while Bifidobacterium breve benefited from facilitative priority effects and utilized a specific degradant to dominate. This study highlights the importance of initial community assembly and its impact on the maturation trajectory of the infant gut microbiota.
Article
Cell Biology
Kyosuke Yakabe, Seiichiro Higashi, Masahiro Akiyama, Hiroshi Mori, Takumi Murakami, Atsushi Toyoda, Yuta Sugiyama, Shigenobu Kishino, Kenji Okano, Akiyoshi Hirayama, Aina Gotoh, Shunyi Li, Takeshi Mori, Takane Katayama, Jun Ogawa, Shinji Fukuda, Koji Hase, Yun-Gi Kim
Summary: A soy protein diet increases the mortality rate of antibiotic-treated, C. difficile-infected mice and enhances the levels of amino acids and abundance of Lactobacillus genus in the intestines. The fermentation of soy protein by Ligilactobacillus murinus generates amino acids that promote the growth of C. difficile, and this process involves the anchored cell wall proteinase PrtP. Thus, the interaction between dietary protein and the gut microbiota is a critical factor affecting host susceptibility to CDI, suggesting that dietary protein sources play an important role in controlling the disease.
Article
Microbiology
Aleksandr A. Arzamasov, Aruto Nakajima, Mikiyasu Sakanaka, Miriam N. Ojima, Takane Katayama, Dmitry A. Rodionov, Andrei L. Osterman
Summary: This study describes the NagR-mediated transcriptional regulation of WI and LNnT utilization in Bifidobacterium longum subsp. infantis. The elucidated regulatory network appears optimally adapted to simultaneous utilization of multiple HMOs, providing a rationale to add HMO mixtures (rather than individual components) to infant formulas. The study also provides insights into the evolutionary trajectories of complex regulatory networks controlling carbohydrate metabolism in bifidobacteria.
Article
Gastroenterology & Hepatology
Yuta Sugiyama, Yumiko Mori, Misaki Nara, Yusuke Kotani, Emiko Nagai, Hiroki Kawada, Mayu Kitamura, Rika Hirano, Hiromi Shimokawa, Akira Nakagawa, Hiromichi Minami, Aina Gotoh, Mikiyasu Sakanaka, Noriho Iida, Takashi Koyanagi, Takane Katayama, Shigefumi Okamoto, Shin Kurihara
Summary: Gut microbiota can produce aromatic amines, which are different from dietary sources. The production of these aromatic amines is related to the activity of aadc in gut bacteria and may have an impact on diseases such as osteoporosis and irritable bowel syndrome. Human AADC inhibitors can inhibit the production of aromatic amines in gut bacteria.
Article
Gastroenterology & Hepatology
Junki Miyamoto, Hidenori Shimizu, Keiko Hisa, Chiaki Matsuzaki, Shinsuke Inuki, Yuna Ando, Akari Nishida, Ayano Izumi, Mayu Yamano, Chihiro Ushiroda, Junichiro Irie, Takane Katayama, Hiroaki Ohno, Hiroshi Itoh, Kenji Yamamoto, Ikuo Kimura
Summary: Fermented foods have health benefits due to probiotic bacteria or microproducts produced by bacterial fermentation. Prebiotics associated with L. mesenteroides-produced exopolysaccharides (EPS) show significant metabolic benefits. EPS-derived gut microbial short-chain fatty acids improve glucose metabolism and energy homeostasis, and alter gut microbial composition.
Article
Biochemistry & Molecular Biology
Hiromi Takada, Toshihiko Katoh, Mikiyasu Sakanaka, Toshitaka Odamaki, Takane Katayama
Summary: Intestinal mucous layers play a crucial role in mediating the symbiotic and dysbiotic interactions between hosts and microbes. This study focuses on the mucin O-glycan degrading ability of gut microbes, specifically Bifidobacterium bifidum, and reveals the important roles of two 11-N-acetylglucosaminidases belonging to the GH20 and GH84 families in mucin O-glycan degradation. Substrate specificity analysis and O-glycomic analysis demonstrate the specific functions of BbhI and BbhIV in breaking down different types of mucin core structures. Additionally, the study uncovers the interdependence of BbhI and BbhIV in efficient hydrolysis of mucin core structures.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Toshihiko Katoh, Chihaya Yamada, Michael D. Wallace, Ayako Yoshida, Aina Gotoh, Moe Arai, Takako Maeshibu, Toma Kashima, Arno Hagenbeek, Miriam N. Ojima, Hiromi Takada, Mikiyasu Sakanaka, Hidenori Shimizu, Keita Nishiyama, Hisashi Ashida, Junko Hirose, Maria Suarez-Diez, Makoto Nishiyama, Ikuo Kimura, Keith A. Stubbs, Shinya Fushinobu, Takane Katayama
Summary: The BbhII enzyme from Bifidobacterium bifidum is involved in the breakdown of mucin O-glycans, modulating host-microbiota symbiosis and dysbiosis.
NATURE CHEMICAL BIOLOGY
(2023)
Review
Multidisciplinary Sciences
Hidehiko Kumagai, Takane Katayama, Takashi Koyanagi, Hideyuki Suzuki
Summary: This article discusses a simple enzymatic synthesis method using bacterial L-tyrosine phenol-lyase (Tpl) for the production of L-DOPA, a treatment for Parkinson's disease. The research covers screening of bacterial strains, culture conditions, properties and reaction mechanism of the enzyme, as well as the reaction conditions for L-DOPA production. Additionally, the article explores molecular breeding of Tpl-over-producing strains through mutations in the DNA binding protein TyrR.
PROCEEDINGS OF THE JAPAN ACADEMY SERIES B-PHYSICAL AND BIOLOGICAL SCIENCES
(2023)
Article
Microbiology
Akira Sen, Tatsuki Nishimura, Shin Yoshimoto, Keisuke Yoshida, Aina Gotoh, Toshihiko Katoh, Yasuko Yoneda, Toyoyuki Hashimoto, Jin-Zhong Xiao, Takane Katayama, Toshitaka Odamaki
Summary: Examining the influence of host cells on the metabolism of probiotics is crucial for understanding the mechanisms underlying the efficacy of probiotics in vivo. However, there is limited research on the interaction between probiotics and host cells, such as intestinal epithelial cells. In this study, a comprehensive metabolome analysis was conducted on a co-culture of Bifidobacterium breve MCC1274 and induced pluripotent stem cells (iPS)-derived small intestinal-like cells, revealing increased levels of amino acid metabolites and gene expression related to ILA synthesis in B. breve MCC1274 cells. The presence of purines produced by intestinal epithelial cells enhanced ILA production, suggesting a synergistic interaction between probiotics and IECs as the molecular basis of host-probiotic interaction in vivo.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Chihaya Yamada, Takane Katayama, Shinya Fushinobu
Summary: Glycoside hydrolase family 136 (GH136) was established based on the discovery and structural analysis of lacto-N-biosidase (LNBase) from Bifidobacterium longum subsp. longum JCM1217 (BlLnbX). Homologous genes of BlLnbX are widely found in human gut bacteria and monkey Bifidobacterium spp. Other bacterial strains like Bifidobacterium saguini, Tyzzerella nexilis, and Ruminococcus lactaris also exhibit LNBase activity. Crystal structures of LNBases from B. saguini and T. nexilis were determined in complex with lacto-N-biose I, showing a conserved fold and catalytic residues.
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
(2022)
