Article
Agricultural Engineering
Xiaojing Zhang, Bingbing Ma, Nan Zhang, Han Zhang, Yongpeng Ma, Yali Song, Hongzhong Zhang
Summary: This study investigated the regulatory effect of the external quorum sensing signals, N-dodecanoyl homoserine lactone (C12-HSL), on the CANON process in a membrane bioreactor. C12-HSL enhanced the activity of aerobic ammonia-oxidizing bacteria and improved ammonia monooxygenase activity, while suppressing anaerobic ammonia-oxidizing bacteria and limiting TN removal. Enzyme synthesis was enhanced, enabling the CANON system to resist high C12-HSL concentrations. C12-HSL was found to be profitable for aerobic ammonia oxidation and could be used to regulate nitrite production.
BIORESOURCE TECHNOLOGY
(2023)
Review
Microbiology
Lanlan Liu, Xiangyong Zeng, Jia Zheng, Yunman Zou, Shuyi Qiu, Yifeng Dai
Summary: This review explores the role of AHL-mediated quorum sensing (AHL-QS) in the regulation of bacterial substance and energy metabolism. The review provides insights into the typical molecular structures, general mechanism, and regulatory mechanisms of AHLs in various metabolic processes.
MICROBIOLOGICAL RESEARCH
(2022)
Article
Microbiology
Liushen Lu, Junwei Wang, Ting Qin, Kai Chen, Jun Xie, Bingwen Xi
Summary: In this study, it was found that carvacrol, a natural compound found in plants, can effectively inhibit bacterial communication and reduce the toxicity of pathogenic bacteria. Carvacrol was shown to reduce the production of violacein in Chromobacterium violaceum and the concentration of N-acyl-homoserine lactones (AHLs) in Aeromonas hydrophila NJ-35. These findings suggest that carvacrol could be a potential strategy for reducing the virulence of pathogenic bacteria.
Article
Engineering, Environmental
Jing Li, He Liu, Ping Wu, Peng Ding, Jie Zhang, Chao Zhang, Bo Fu, Min-hua Cui
Summary: This study investigates the enhancement of caproate production from ethanol and acetate in chain elongation enrichments by utilizing AHLs as signal molecules. The results show that adding C8-HSL significantly increases caproate concentration, possibly due to the increase in protein content and total biomass.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2021)
Article
Engineering, Marine
Gary E. Schultz, Peter H. Santschi
Summary: Free-living bacteria in marine environments are believed to be too sparse to use density-dependent quorum sensing systems. However, this study found that marine bacteria do respond to signal molecules, indicating the presence of quorum sensing in marine gels. This discovery is significant for marine and environmental sciences.
JOURNAL OF MARINE SCIENCE AND ENGINEERING
(2023)
Review
Biochemistry & Molecular Biology
Qiang Zhang, Sizhe Li, Maha Hachicha, Mohamed Boukraa, Laurent Soulere, Mohamed L. Efrit, Yves Queneau
Summary: This review summarizes the contribution of heterocyclic chemistry in the design of AHLs analogues, emphasizing how heterocyclic building blocks can serve as replacements of the lactone moiety, as a bioisostere for the amide group, or as an additional pattern appended to the side chain. Additionally, non-AHL-related heterocyclic compounds with AHL-like QS activity are also mentioned.
Article
Environmental Sciences
Jie Cheng, Xin Su, Meng Liu, Zhijiang Lu, Jianming Xu, Yan He
Summary: This study investigated the effects of exogenous N-butanoyl homoserine lactone (C4-HSL), N-hexanoyl homoserine lactone (C6-HSL), and 3-oxo-hexanoyl homoserine lactone (3OC6-HSL) on the dechlorination of ?-hexachlorocyclohexane (?) and methane production. The results showed that exogenous C4-HSL and 3OC6-HSL increased cytochrome c production, enriched dechlorinators and electroactive bacteria, and accelerated dechlorination while inhibiting methane production. On the other hand, C6-HSL facilitated dechlorination and methane production by enhancing biofilm electroactivity and increasing membrane transportation.
ENVIRONMENTAL POLLUTION
(2023)
Article
Environmental Sciences
M. de Celis, L. Serrano-Aguirre, I Belda, R. Liebana-Garcia, M. Arroyo, D. Marquina, I de la Mata, A. Santos
Summary: The study indicates that the use of AHLs-degrading enzymes can reduce biofilm formation on WWTP membranes and ameliorate the virulence of Gram-negative bacteria.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Review
Plant Sciences
Xixian Zhu, Wen-Juan Chen, Kalpana Bhatt, Zhe Zhou, Yaohua Huang, Lian-Hui Zhang, Shaohua Chen, Junxia Wang
Summary: With the increasing resistance of undesirable bacteria to traditional antibiotics, the need to discover alternative treatments is urgent. Quorum sensing is a novel bacterial communication system mediated by signaling molecules called autoinducers. Quorum quenching has been developed as a strategy to inhibit quorum sensing and control resistant bacteria. This review introduces different signaling molecules, elucidates the mechanisms of quorum quenching, and summarizes the applications and advantages of this strategy.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Environmental Sciences
Yi Zhang, Jing Zhang, Jun Li, Pengyuan Wei, Rong Luo, Hao Han
Summary: By adding enhanced ANAMMOX granules (LT-granules) into the inoculated denitrification sludge, the fast start-up of ANAMMOX biofilm processes at low temperatures (18 ± 3℃) was achieved in this study.
ENVIRONMENTAL RESEARCH
(2022)
Article
Engineering, Environmental
Hong Wang, Peike Wu, Dan Zheng, Liangwei Deng, Wenguo Wang
Summary: N-acyl-homoserine lactones (AHLs) play an important role in quorum sensing regulation of Gram-negative bacteria, positively influencing the production of aromatic proteins in extracellular polymeric substances (EPSs). Addition of exogenous C12-HSL enhances flocculation activity and aromatic protein production in a Chlorella-bacteria system, while the quorum sensing inhibitor vanillin suppresses these effects.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Nathan Grellier, Marcelino T. Suzuki, Loic Brot, Alice M. S. Rodrigues, Lydie Humbert, Karine Escoubeyrou, Dominique Rainteau, Jean-Pierre Grill, Raphael Lami, Philippe Seksik
Summary: The study found that the luxR gene from Bacteroides dorei was under-expressed in IBD patients, while one luxR receptor gene from Bacteroides fragilis appeared to be over-expressed. Targeting LuxR receptors of bacterial quorum sensing might represent a new approach to modulate the gut microbiota in IBD.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Environmental Sciences
Peihan Zheng, Qian Zhang, Jiaxing Zou, Qi Han, Jiarong Han, Qixin Wang, Liting Yao, Guangwei Yu, Yuhai Liang
Summary: Under ambient temperature and high dissolved oxygen, different types of acyl-homoserine lactones were added to wastewater nitrification systems, leading to the enrichment of AOA. The relative abundance of AOA increased from undetected to 1.10% and 0.96% respectively. The exogenous signaling molecules provide a new perspective for AOA enrichment in wastewater treatment systems.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Biotechnology & Applied Microbiology
Rachith Kalgudi, Roya Tamimi, Godfrey Kyazze, Tajalli Keshavarz
Summary: The study revealed that the combined use of farnesol and tyrosol had a better effect on Pseudomonas aeruginosa biofilm growth, especially against the RBHi strain, while the addition of quorum quenchers (QQs) reduced IL-8 secretion by A549 cells.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Environmental Sciences
Zhen Tong, Yong-Chao Wang, Guan-Yu Jiang, Xu-Rui Hu, Yi-Mei Xue, Can Wang
Summary: A 96-well plate assay based on a specific biosensor and a colorimetric substance was developed to detect the QQ activities of biofilm samples. This method improved the accuracy of QQ activity detection and reduced time and cost consumption.
Article
Biochemistry & Molecular Biology
Jennifer Harrow, John Hancock, Niklas Blomberg
Article
Microbiology
Terje Klemetsen, Christian R. Karlsen, Nils P. Willassen
Summary: Genus Aliivibrio, known for bioluminescence and pathogenic behavior affecting fish farming, has been reclassified and poorly understood phylogenetically since 2007. Through MLSA with 143 bacterial strains, this study identified 12 clades and proposed a new species, Aliivibrio sp. friggae. Marker gene analysis revealed gyrB, pyrH, and recA as superior to 16S rRNA for accurate species classification in Aliivibrio.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Mitchell R. Silk, Jason R. Price, Biswaranjan Mohanty, Hanna-Kirsti S. Leiros, Bjarte A. Lund, Philip E. Thompson, David K. Chalmers
Summary: This study investigates the impact of side chain alignment, hydrophobicity, and charge on the stability of cyclic d/l peptide (CP) nanotubes, revealing that hydrophobic amino acids enhance CP dimer stability while charged amino acids' effect depends on the orientation and composition of charged groups. X-ray crystallography, NMR spectroscopy, and MD simulations are utilized to characterize the factors influencing CP nanotube stability.
CHEMISTRY-A EUROPEAN JOURNAL
(2021)
Article
Multidisciplinary Sciences
Jolyn Pan, Kjersti Lian, Aili Sarre, Hanna-Kirsti S. Leiros, Adele Williamson
Summary: DNA ligases, enzymes that join breaks in the DNA backbone, vary in size and structure. Bacterial ligases show closer clustering with Eukaryote and Archaeal ligases, while certain bacterial ligases are more similar to viral sequences. Further analysis revealed distinct groups including bacterial Lig E sequences, T7 DNA ligase homologs, and Chlorella virus ligase homologs with both bacterial and viral enzymes. Investigation of the bacterially-encoded Chlorella virus homologs showed similarities in activity and structure to eukaryotic virus Chlorella virus, suggesting a potential entry point for horizontal acquisition of ATP-dependent DNA ligases by bacteria.
SCIENTIFIC REPORTS
(2021)
Article
Multidisciplinary Sciences
Amudha Deepalakshmi Maharajan, Hilde Hansen, Miriam Khider, Nils Peder Willassen
Summary: A. wodanis 06/09/139 utilizes the QS system to regulate various activities that may be important for host colonization or interactions. The production of 3OHC10-HSL by the bacterium is highest at a low temperature of 6 degrees Celsius.
Article
Biochemical Research Methods
Bjarte Aarmo Lund, Ane Molden Thomassen, Trine Josefine Warg Carlsen, Hanna-Kirsti Schroder Leiros
Summary: The crystal structure and catalytic properties of the class D beta-lactamase OXA-436 were studied, revealing its superior adaptation compared to OXA-48 and higher catalytic rates for the antibiotic imipenem. While conformational shifts were observed, they did not result in clinically significant resistance profile changes, suggesting a continuum in antibiotic-resistance enzymes.
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS
(2021)
Article
Biotechnology & Applied Microbiology
Mikkel Christensen, Piotr Jablonski, Bjorn Altermark, Knut Irgum, Hilde Hansen
Summary: In this study, two marine Cobetia strains were characterized for their production of polyhydroxyalkanoates (PHA). Both strains showed high PHA production from pure substrates, highlighting their potential as natural PHA producers. Further scale up, optimization of growth conditions, or metabolic engineering is needed for achieving industrially relevant PHA production titers. Additionally, genomic analysis revealed differences in PHA biosynthesis genes and metabolic pathways between the two strains, indicating the need for further research to improve PHA production yields.
MICROBIAL CELL FACTORIES
(2021)
Article
Chemistry, Multidisciplinary
Katarzyna Palica, Manuela Voracova, Susann Skagseth, Anna Andersson Rasmussen, Lisa Allander, Madlen Hubert, Linus Sandegren, Hanna-Kirstirep Schroder Leiros, Hanna Andersson, Mate Erdelyi
Summary: Infectious diseases remain a major concern for public health in the 21st century, and the development of antimicrobial resistance by microbes is a significant problem. The study found that phosphonamidate compounds have the potential to become a new generation of antibiotic therapeutics to combat metallo-beta-lactamase-resistant bacteria.
Article
Veterinary Sciences
Amudha Deepalakshmi Maharajan, Erik Hjerde, Hilde Hansen, Nils Peder Willassen
Summary: To survive and compete in a crowded and challenging environment, bacteria like Aliivibrio wodanis 06/09/139 employ different strategies, such as Type VI Secretion Systems (T6SSs) and Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR), to outcompete their competitors. This study analyzed the genome and gene expression profiling of A. wodanis and two QS mutants (Delta ainS and Delta litR) to understand the role of quorum sensing (QS) and these strategies. The findings showed that T6SSs and CRISPR systems are QS-dependent and play a crucial role in the survival of A. wodanis in its natural environment.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Biochemical Research Methods
Fernando Meyer, Adrian Fritz, Zhi-Luo Deng, David Koslicki, Till Robin Lesker, Alexey Gurevich, Gary Robertson, Mohammed Alser, Dmitry Antipov, Francesco Beghini, Denis Bertrand, Jaqueline J. Brito, C. Titus Brown, Jan Buchmann, Aydin Buluc, Bo Chen, Rayan Chikhi, Philip T. L. C. Clausen, Alexandru Cristian, Piotr Wojciech Dabrowski, Aaron E. Darling, Rob Egan, Eleazar Eskin, Evangelos Georganas, Eugene Goltsman, Melissa A. Gray, Lars Hestbjerg Hansen, Steven Hofmeyr, Pingqin Huang, Luiz Irber, Huijue Jia, Tue Sparholt Jorgensen, Silas D. Kieser, Terje Klemetsen, Axel Kola, Mikhail Kolmogorov, Anton Korobeynikov, Jason Kwan, Nathan LaPierre, Claire Lemaitre, Chenhao Li, Antoine Limasset, Fabio Malcher-Miranda, Serghei Mangul, Vanessa R. Marcelino, Camille Marchet, Pierre Marijon, Dmitry Meleshko, Daniel R. Mende, Alessio Milanese, Niranjan Nagarajan, Jakob Nissen, Sergey Nurk, Leonid Oliker, Lucas Paoli, Pierre Peterlongo, Vitor C. Piro, Jacob S. Porter, Simon Rasmussen, Evan R. Rees, Knut Reinert, Bernhard Renard, Espen Mikal Robertsen, Gail L. Rosen, Hans-Joachim Ruscheweyh, Varuni Sarwal, Nicola Segata, Enrico Seiler, Lizhen Shi, Fengzhu Sun, Shinichi Sunagawa, Soren Johannes Sorensen, Ashleigh Thomas, Chengxuan Tong, Mirko Trajkovski, Julien Tremblay, Gherman Uritskiy, Riccardo Vicedomini, Zhengyang Wang, Ziye Wang, Zhong Wang, Andrew Warren, Nils Peder Willassen, Katherine Yelick, Ronghui You, Georg Zeller, Zhengqiao Zhao, Shanfeng Zhu, Jie Zhu, Ruben Garrido-Oter, Petra Gastmeier, Stephane Hacquard, Susanne Haeussler, Ariane Khaledi, Friederike Maechler, Fantin Mesny, Simona Radutoiu, Paul Schulze-Lefert, Nathiana Smit, Till Strowig, Andreas Bremges, Alexander Sczyrba, Alice Carolyn McHardy
Summary: This study presents the results of the second round of the Critical Assessment of Metagenome Interpretation challenges (CAMI II), which is a community-driven effort for comprehensively benchmarking tools for metagenomics data analysis. The results show substantial improvements in assembly, but related strains and assembly quality still pose challenges. Taxon profilers and binners excel at higher bacterial ranks but underperform for viruses and Archaea. The need to improve reproducibility is emphasized by the clinical pathogen detection results.
Article
Genetics & Heredity
Betty M. N. Furulund, Bard O. Karlsen, Igor Babiak, Peik Haugen, Steinar D. Johansen
Summary: Group I introns in myxomycetes, a distinct protist phylum, exhibit dynamic structures and complex inheritance patterns. The consensus secondary structure reveals a conserved core and RNA sequence complexity in the peripheral regions. These introns possess various structural organizations, including twintron organization and direct repeat arrays. Phylogenetic analyses indicate both vertical and horizontal transfers. The evolutionary history involves the insertion of mobile-type introns, degradation of homing endonuclease genes, and ultimately complete loss of the introns.
Article
Biochemical Research Methods
Netsanet Gizaw Assefa, Hilde Hansen, Bjorn Altermark
Summary: Polyhydroxyalkanoates (PHAs) are natural biodegradable polyesters produced by microorganisms. This study identified and characterized a unique PHA biosynthesis enzyme, PhaC (BrPhaC), from a marine bacterium. Unlike other enzymes, BrPhaC is a trimeric enzyme without a lag phase and initiates enzymatic reaction instantly, regardless of temperature.
Article
Genetics & Heredity
Kjersti Lian, Betty M. N. Furulund, Anders A. Tveita, Peik Haugen, Steinar D. Johansen
Summary: The study analyzed nuclear L2066 group I introns from myxomycetes and ascomycetes, revealing the presence of mobile-type introns with homing endonuclease genes. In vitro and in vivo experiments demonstrated the mechanism of double-strand break at the DNA insertion site by these introns.
Article
Microbiology
Christopher Frohlich, Joao A. Gama, Klaus Harms, Viivi H. A. Hirvonen, Bjarte A. Lund, Marc W. van der Kamp, Pal J. Johnsen, Orjan Samuelsen, Hanna-Kirsti S. Leiros
Summary: Low concentrations of beta-lactam antibiotics can enhance beta-lactamase diversity and significantly influence substrate specificity, driving the evolution of beta-lactamases and potentially contributing to the diversification and divergence of these enzymes when antibiotic pressure increases.
