期刊
JOURNAL OF APPLIED MICROBIOLOGY
卷 114, 期 5, 页码 1369-1377出版社
WILEY-BLACKWELL
DOI: 10.1111/jam.12141
关键词
BrnFE; Corynebacterium glutamicum; L-isoleucine production; Lrp; pDXW-8
资金
- National key Basic Research Program of China [2012CB725202]
- Basic Research Programs of Jiangsu Province [BK2009003]
- 111 Project [111-2-06]
Aims: To increase the L-isoleucine production in Corynebacterium glutamicum by overexpressing the global regulator Lrp and the two-component export system BrnFE. Methods and Results: The brnFE operon and the lrp gene were cloned into the shuttle vector pDXW-8 individually or in combination. The constructed plasmids were transformed into an L-isoleucine-producing strain C. glutamicum JHI3-156, and the L-isoleucine production in these different strains was analysed and compared. More L-isoleucine was produced when only Lrp was expressed than when only BrnFE was expressed. Significant increase in L-isoleucine production was observed when Lrp and BrnFE were expressed in combination. Compared to the control strain, L-isoleucine production in JHI3-156/pDXW-8-lrp-brnFE increased 63% in flask cultivation, and the specific yield of L-isoleucine increased 72% in fed-batch fermentation. Conclusions: Both Lrp and BrnFE are important to enhance the L-isoleucine production in C. glutamicum. Significance and Impact of the Study: The results provide useful information to enhance L-isoleucine or other branched-chain amino acid production in C. glutamicum.
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