Article
Biochemical Research Methods
Shiyu He, Yanzhi Huang, Yingwei Ma, Haoyan Yu, Bo Pang, Xingxing Liu, Caihong Yin, Xiaomu Wang, Yuan Wei, Yuling Tian, Chao Zhao, Kun Xu, Juan Wang, Chunping Lv, Xiuling Song, Minghua Jin
Summary: A new detection system using magnetic separation, MPCR, and CE technologies was developed to simultaneously detect four foodborne pathogens. The combination of magnetic separation, MPCR, and CE greatly increased detection sensitivity, with a detection limit for bacterial DNA of 10(-5)-10(-7)ng mu L-1. This technique showed good sensitivity and specificity in detecting bacteria in food samples, without the need for time-consuming enrichment cultures.
BIOTECHNOLOGY JOURNAL
(2022)
Article
Food Science & Technology
Eun-Ji Shin, Soo-Jeong Lee, Hoon-Jae Jeong, Ju-Hoon Lee
Summary: A novel multiplex real-time PCR method was developed for rapid and accurate serogrouping of Escherichia coli O-serotypes, enabling identification in food environments.
Article
Food Science & Technology
Pengzhen Li, Xiaoxuan Feng, Baiyan Chen, Xiaoying Wang, Zuyue Liang, Li Wang
Summary: A multiplex PCR system was developed for simultaneous detection of eight foodborne pathogenic bacteria. The system exhibited high sensitivity and specificity, and showed good stability and sensitivity in artificially contaminated samples.
Article
Food Science & Technology
Natharin Ngamwongsatit, Soraya Chaturongakul, Ratchaneewan Aunpad
Summary: Microbial contamination in foods can cause illnesses and significant losses in both the food industry and public health sectors. This study developed a multiplex PCR (m-PCR) assay for the rapid and reliable detection of six common foodborne pathogens and hygiene indicator microorganisms. The m-PCR showed comparable results to the gold-standard culture technique but had a significantly shorter processing time.
FOODBORNE PATHOGENS AND DISEASE
(2023)
Article
Food Science & Technology
Mei Xie, Tianlan Chen, Xiong Xin, Zongwei Cai, Cheng Dong, Bo Lei
Summary: A method using a digital microfluidic chip and loop-mediated isothermal amplification technology was developed for multiplex detection of foodborne pathogens. The method showed higher sensitivity, simpler operation, higher efficiency, and smaller size compared to the commercial benchtop LAMP method.
Article
Microbiology
Dong-Geun Park, Joon-Gi Kwon, Eun-Su Ha, Byungcheol Kang, Iseul Choi, Jeong-Eun Kwak, Jinho Choi, Woojung Lee, Seung Hwan Kim, Soon Han Kim, Jeongwoong Park, Ju-Hoon Lee
Summary: This study developed new NGS panel primer sets targeting 18 specific virulence factor genes from six target pathogens and validated their specificity and selectivity through PCR. The NGS panel analysis detected all target genes in spiked water samples, showing a positive correlation between gene concentration and pathogen density. Comparative analysis with qPCR confirmed the efficiency and specificity of the NGS panel analysis. Further optimization of primer sets and real-time NGS sequencing technology can enhance the detection capability of NGS panel analysis.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Biology
Hisham N. Altayb, Rania M. Badri, Kamel Chaieb, Ehssan Moglad
Summary: Food microbial contamination is a serious problem. This study used PCR to identify the most common foodborne pathogens in food samples from Khartoum state. The presence of Listeria monocytogenes and Vibrio cholerae in raw milk and fresh cheese samples highlights the need for enhanced hygiene measures.
SAUDI JOURNAL OF BIOLOGICAL SCIENCES
(2023)
Review
Microbiology
Mohammed Aladhadh
Summary: Despite advances in food preservation and safety, foodborne diseases caused by bacteria, fungi, and viruses still pose significant risks to public health. Previous reviews on foodborne pathogen detection methods have focused mainly on bacteria, neglecting the importance of viruses. This holistic review discusses various methods for detecting bacterial, fungal, and viral pathogens in food, including culture-based methods, immunoassays, PCR, and next-generation sequencing. Utilizing these tools can lead to early detection and control of foodborne diseases, reducing the frequency of outbreaks and enhancing public health.
Article
Microbiology
Yipu Du, Ziheng Yan, Kai Song, Junyan Jin, Liting Xiao, Zhulin Sun, Yafang Tan, Pingping Zhang, Zongmin Du, Ruifu Yang, Yong Zhao, Yajun Song
Summary: Biothreat agents pose a huge threat to human and public health. This study establishes a multiplex droplet digital polymerase chain reaction (ddPCR) method for simultaneously detecting five high-risk bacterial biothreats. The developed ddPCR method is more sensitive than conventional methods and is potentially suitable for rapidly detecting or screening the selected bacterial biothreats in suspicious samples.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Food Science & Technology
Zhendi Yu, Zhangkai Xu, Jiang Chen, Lili Chen, Ningbo Liao, Ronghua Zhang, Dongqing Cheng
Summary: Foodborne diseases are a critical food safety issue globally, with shellfish carrying various foodborne viruses posing a risk of infection. Factors such as age, season, and type of shellfish are associated with different levels of infection risk.
Article
Chemistry, Analytical
Shuo Yao, Bo Pang, Yanli Fu, Xiuling Song, Kun Xu, Juan Li, Juan Wang, Chao Zhao
Summary: In this study, a quantitative assay using ICP-MS, magnetic separation, and metal nanoclusters-mediated signal amplification was developed for the simultaneous detection of multiple foodborne pathogens. The method showed a high sensitivity, being able to detect three target pathogens as low as 10 CFU/mL in a short time. It was successfully applied to the determination of target bacteria in food samples.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Food Science & Technology
Qian Xu, Siyuan Liu, Shunshi Ji, Ziquan Wang, Meng Wang, Yingying Liu, Xiaoyun Gong, Boqiang Fu, Changyun Ye, Haiyan Chang, Zhiwei Sui
Summary: This study reports a flow cytometry-based method for rapid and multiplexed quantification of Listeria monocytogenes, Salmonella spp., and E. coli O157:H7 in chicken breast. The developed method is efficient, reproducible, and has the potential to be applied in food contamination monitoring.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Food Science & Technology
Xiaoying Han, Yuanhui Liu, Juxin Yin, Min Yue, Ying Mu
Summary: The global burden of foodborne diseases is significant, with foodborne pathogens being the major cause of human illnesses. Microfluidic technology enables rapid, cost-effective, sensitive, and portable pathogen detection to meet on-site testing requirements. Multiplexed detection technology offers a new opportunity to ensure food safety by simultaneously detecting multiple pathogens.
FOOD RESEARCH INTERNATIONAL
(2021)
Article
Clinical Neurology
Yanjun Si, Weijun He, Shuo Guo, Xiaohui Wang, Meng Tang, Binwu Ying, Minjin Wang
Summary: This study investigated the clinical utility and epidemiological characteristics of ME in southwestern China using a novel multiplex PCR detection method. The results showed that MME-18 had good consistency with conventional testing for ME diagnosis, aiding in early pathogen identification.
FRONTIERS IN NEUROLOGY
(2022)
Article
Multidisciplinary Sciences
Li Sun, David G. Meckes
Summary: The new immuno-PCR method utilizes TotalSeq antibodies containing DNA conjugated oligos for high sensitivity and specificity profiling of multiple EV surface proteins simultaneously. This method has been successfully applied to cell and serum samples, and a micro-size exclusion chromatography method has been developed to detect EV surface proteins in as little as 10 μl of human serum when combined with immuno-PCR. Overall, this approach allows for rapid detection of multiple EV markers from small sample volumes in a single tube.
SCIENTIFIC REPORTS
(2021)
Article
Biotechnology & Applied Microbiology
Y. Zhao, A. Zhu, J. Tang, C. Tang, J. Chen, J. Liu
JOURNAL OF APPLIED MICROBIOLOGY
(2016)
Article
Microbiology
Juan Chen, Junni Tang, Hui Shi, Cheng Tang, Rong Zhang
JOURNAL OF BASIC MICROBIOLOGY
(2017)
Article
Food Science & Technology
Juan Chen, Junni Tang, Cheng Tang, Hui Shi, Huaiyin Gu, Xiaozhu Lu
JOURNAL OF FOOD QUALITY
(2016)
Article
Food Science & Technology
Yanying Zhao, Anni Zhu, Junni Tang, Cheng Tang, Juan Chen
JOURNAL OF FOOD QUALITY
(2017)
Article
Biotechnology & Applied Microbiology
Y. Zhao, A. Zhu, J. Tang, C. Tang, J. Chen
LETTERS IN APPLIED MICROBIOLOGY
(2017)
Article
Food Science & Technology
Yisalan Ma, Yanying Zhao, Junni Tang, Cheng Tang, Juan Chen, Ji Liu
CYTA-JOURNAL OF FOOD
(2018)
Article
Cell Biology
Jingqi Ren, Yaqiu Lin, Junni Tang, Hua Yue, Yanying Zhao
CELLULAR PHYSIOLOGY AND BIOCHEMISTRY
(2018)
Article
Agriculture, Dairy & Animal Science
J. Chen, J. N. Tang, K. L. Hu, Y. Y. Zhao, C. Tang
JOURNAL OF DAIRY SCIENCE
(2018)
Article
Biotechnology & Applied Microbiology
Yanying Zhao, Junni Tang
JOURNAL OF FOOD SAFETY
(2018)
Article
Biotechnology & Applied Microbiology
Yanying Zhao, Junni Tang, Cheng Tang
JOURNAL OF FOOD SAFETY
(2020)
Article
Agriculture, Dairy & Animal Science
Yanying Zhao, Junni Tang, Danru Yang, Cheng Tang, Juan Chen
JOURNAL OF DAIRY SCIENCE
(2020)
Article
Food Science & Technology
Yanying Zhao, Yun Qu, Junni Tang, Juan Chen, Ji Liu
Summary: The study revealed that tea catechin extracts effectively suppressed the growth and biofilm formation of MRSA strains, possibly through downregulating the expression of fnbA and icaBC genes.
JOURNAL OF FOOD QUALITY
(2021)
Article
Food Science & Technology
Chenglin Zhu, Kaiwei Tang, Xuan Lu, Junni Tang, Luca Laghi
Summary: This study characterized the metabolome of mastitic milk using untargeted nuclear magnetic resonance spectroscopy, identifying 54 molecules and exploring the mechanisms of milk metabolome variation affected by clinical mastitis. The results offer a deeper understanding of how clinical mastitis alters milk metabolome, serving as a reference for future research in this area.
Correction
Agriculture, Dairy & Animal Science
Yanying Zhao, Junni Tang, Danru Yang, Cheng Tang, Juan Chen
JOURNAL OF DAIRY SCIENCE
(2022)
Article
Food Science & Technology
Chenglin Zhu, Zhibo Yang, Xuan Lu, Yuwen Yi, Qing Tian, Jing Deng, Dan Jiang, Junni Tang, Luca Laghi
Summary: In recent years, there has been a significant increase in the interest for pear cider as a means to utilize fruits that are unsuitable for direct consumption or to promote pear varieties grown in restricted regions. This study analyzed the taste characteristics and metabolomic profiles of pear cider produced from China's Guangan honey pear by using four different commercial Saccharomyces cerevisiae strains. The results showed that different yeast strains led to distinct changes in metabolic pathways during fermentation, and the study provided valuable insights for improving the quality of pear cider and its large-scale production.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)