Article
Engineering, Environmental
Wei-Cheng Hung, Megyn Rugh, Marina Feraud, Sumant Avasarala, Jessica Kurylo, Mathew Gutierrez, Karina Jimenez, Nhi Truong, Patricia A. Holden, Stanley B. Grant, Haizhou Liu, Richard F. Ambrose, Jennifer A. Jay
Summary: The study found significant correlations among relative gene abundances, total metal(loid)s, and bioavailable metal(loid)s. Soil texture, organic matter, and nutrients within GSI were significantly correlated with relative gene abundances of specific ARGs. Multiple regression models improved the estimation of ARGs in GSI when considering the effects of soil characteristics and metal(loid)s, with total arsenic being a significant correlate in all regression models.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Article
Multidisciplinary Sciences
Zane T. Laughlin, Suparno Nandi, Debayan Dey, Natalia Zelinskaya, Marta A. Witek, Pooja Srinivas, Ha An Nguyen, Emily G. Kuiper, Lindsay R. Comstock, Christine M. Dunham, Graeme L. Conn
Summary: Changes in bacterial ribosomal RNA methylation can have significant impact on antibiotic activity and drug resistance. This study investigates the role of the methyltransferase TlyA in antibiotic resistance in Mycobacterium tuberculosis. The researchers report the structure of a complex between the mycobacterial 50S subunit and TlyA, revealing critical interactions involved in substrate recognition and positioning of the active site. They also find that TlyA interacts with the 30S subunit using the same interaction surface but with different dependence on conserved residues.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Microbiology
Valwynne Faulkner, Adrienne Adele Cox, Shan Goh, Annelies van Bohemen, Amanda J. Gibson, Oliver Liebster, Brendan W. Wren, Sam Willcocks, Sharon L. Kendall
Summary: CRISPRi-based gene silencing can be as impactful as gene deletion for studying non-essential genes, and can be applied to other non-essential antimicrobial resistance genes. Designing sgRNAs targeting transcription initiation or early elongation stages has the most impact on rifampicin susceptibility.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Environmental Sciences
Ruijie Zhang, Xuming Xu, Yitao Lyu, Ying Zhou, Qian Chen, Weiling Sun
Summary: This study investigated the effects of antibiotics and engineered nanoparticles on denitrification in natural aquatic environment. The results showed that antibiotics inhibited the reduction of NO3--N, while engineered nanoparticles promoted denitrification. In binary systems, engineered nanoparticles alleviated the inhibitory effect of antibiotics. The addition of antibiotics and/or engineered nanoparticles also changed the bacterial community structure.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2022)
Article
Environmental Sciences
Min -Ji Kim, Dayun Kang, Gyudae Lee, Kyeongnam Kim, Jinnam Kim, Jae-Ho Shin, Seungjun Lee
Summary: Cyanobacterial harmful algal blooms (cyanoHABs) in freshwater environments are a growing concern for both the environment and public health. This study explores the interactions between cyanoHABs, antibiotic resistance genes (ARGs), and potential synergistic pollutants. The results show that during cyanoHABs, certain bacteria dominate the freshwater environment while ARG carriers decrease. However, mobile gene elements and virulence factors increase. The study highlights the intricate interplays between microbial dysbiosis and ARGs, emphasizing the need for prevention.
ENVIRONMENT INTERNATIONAL
(2023)
Article
Environmental Sciences
Silvia Bonetta, Andrea Di Cesare, Cristina Pignata, Raffaella Sabatino, Manuela Macri, Gianluca Corno, Marco Panizzolo, Sara Bonetta, Elisabetta Carraro
Summary: This study examines the fate of antibiotic resistance in the urban water cycle in Italy, focusing on the prevalence of antibiotic-resistant bacteria (ARB) and antibiotic-resistant genes (ARGs) in wastewater treatment plants (WWTPs) and drinking water treatment plants (DWTPs). Plate counting and quantitative real-time PCR (qPCR) were used to quantify ARB (resistant to tetracycline, ampicillin, and sulfonamides) and measure the abundance of selected ARGs. The results show higher concentrations of ARB and ARGs in WWTPs compared to DWTPs, indicating that WWTPs are hotspots for antibiotic resistance spread. Although significant reductions were observed after treatment, none of the detected ARB or ARGs were completely removed from drinking water. Therefore, it is important to integrate culture-dependent and culture-independent methods to investigate antibiotic resistance dynamics in aquatic ecosystems involved in the urban water cycle, and to monitor the presence of ARB and ARGs, especially in drinking water, as it represents a potential route of transmission to humans.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Review
Environmental Sciences
Rui Zhang, Shu Yang, Yuwei An, Yangqing Wang, Yu Lei, Liyan Song
Summary: Landfill serves as important reservoirs for antibiotics and antibiotic resistance genes, with risks of transferring to the local environment. Studies have shown complex linkages among antibiotics, ARGs, and bacterial communities, highlighting the need for further research.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Microbiology
Jaycee Cushman, Emma Freeman, Sarah McCallister, Anna Schumann, Keith W. Hutchison, Sally D. Molloy
Summary: This study provides evidence that prophages alter the expression of important mycobacterial intrinsic antibiotic resistance genes and may play a role in mycobacterial adaptation to stress. Strains carrying the prophage McProf demonstrated increased resistance to amikacin, which was further enhanced by exposure to acivicin or the presence of a second prophage, BPs. Increased expression of the virulence gene whiB7 was observed in strains carrying both prophages compared to strains carrying a single prophage or no prophages.
Article
Microbiology
Sina Moghaddam, Farshad Nojoomi, Arasb Dabbagh Moghaddam, Mojgan Mohammadimehr, Fatemeh Sakhaee, Morteza Masoumi, Seyed Davar Siadat, Abolfazl Fateh
Summary: This study aimed to evaluate the prevalence of non-tuberculous mycobacteria (NTM) in the drinking water of six hospitals in Tehran, Iran. The study found that NTM strains could be an important component of hospital water supplies and a possible source of nosocomial infections, with drug susceptibility testing results indicating diversity of resistance to different drugs among NTM isolates.
Article
Microbiology
Jodie A. Schildkraut, Jordy P. M. Coolen, Sophie Burbaud, Jasper J. N. Sangen, Michael P. Kwint, R. Andres Floto, Huub J. M. op den Camp, Lindsey H. M. Te Brake, Heiman F. L. Wertheim, Kornelia Neveling, Wouter Hoefsloot, Jakko van Ingen
Summary: This study provides the first description of the major transcriptional mechanisms of tolerance to recommended antibiotics in Mycobacterium abscessus. The bacterium shows both drug-specific and common transcriptomic responses to antibiotic exposure. The findings have significant implications for understanding antibiotic resistance mechanisms and developing targeted therapeutic regimens.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Microbiology
S. Salini, Sinchana G. Bhat, Saba Naz, Ramanathan Natesh, R. Ajay Kumar, Vinay Kumar Nandicoori, Krishna Kurthkoti
Summary: Applying antibiotics to susceptible bacterial cultures generates persistent bacteria that remain susceptible to antibiotics but can endure them. Recent reports have found that antibiotic persisters of mycobacteria experience oxidative stress and develop resistance. Our study reveals that mycobacterial persisters activate the SOS response and upregulate the error-prone DNA polymerase DnaE2, leading to mutagenesis and rapid evolution of antibiotic resistance.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Biotechnology & Applied Microbiology
Abdelazeem M. Algammal, Reham M. El-Tarabili, Wafaa A. Abd El-Ghany, Enas A. Almanzalawi, Tahani M. Alqahtani, Hanaa Ghabban, Amenah S. Al-otaibi, Nayera M. Alatfeehy, Naira M. Abosleima, Helal F. Hetta, Ghada A. Badawy
Summary: This study investigated the emergence of Salmonella Enteritidis and Salmonella Typhimurium in diseased broilers, revealing their extensive drug resistance and potential threat to public health.
Article
Environmental Sciences
Yuqing Mao, Neslihan Akdeniz, Thanh H. Nguyen
Summary: This study compared the abundances of genetic markers of opportunistic foodborne and airborne pathogens in backyard, commercial, and swine mortality composts. The findings suggest that backyard composts have lower abundances of antibiotic resistance genes, while airborne pathogen genes are present in various compost samples to some extent.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Agricultural Engineering
Yiqi Wu, Zhiqiang Chen, Qinxue Wen, Qiqi Fu, Huanyu Bao
Summary: The study demonstrated that ozone treatment significantly reduced ARGs and MGEs in composting product, with the changes in other ARGs post-ozonation related to MGEs abundance. Additionally, the reduction of potential host bacteria after ozonation contributed to the removal of intracellular ARGs.
BIORESOURCE TECHNOLOGY
(2021)
Article
Environmental Sciences
Andrea Di Cesare, Lee J. Pinnell, Diego Brambilla, Giulia Elli, Raffaella Sabatino, Maria B. Sathicq, Gianluca Corno, Colin O'Donnell, Jeffrey W. Turner
Summary: Research has shown that bioplastics accumulate antibiotic and metal resistance genes in marine sediments, with the PHA group being particularly enriched in certain ARGs and MRGs. There is a strong correlation between the relative abundance of ARGs and MRGs, and PHA biofilms were found to be colonized by antibiotic-resistant hemolytic Bacillus cereus group bacteria.
ENVIRONMENTAL POLLUTION
(2021)
Article
Biotechnology & Applied Microbiology
Chi Keung Cheng, Man Kit Cheung, Wenyan Nong, Patrick Tik Wan Law, Jing Qin, Julia Mei-Lun Ling, Kai Man Kam, William Man Wai Cheung, Hoi Shan Kwan
Article
Infectious Diseases
Xichao Ou, Hui Xia, Qiang Li, Yu Pang, Shengfen Wang, Bing Zhao, Yuanyuan Song, Yang Zhou, Yang Zheng, Zhijian Zhang, Zhiying Zhang, Junchen Li, Haiyan Dong, Junying Chi, Jack Zhang, Kai Man Kam, Shitong Huan, Yue Jun, Daniel P. Chin, Yanlin Zhao
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2015)
Article
Infectious Diseases
Joyce H. S. You, Grace Lui, Kai Man Kam, Nelson L. S. Lee
JOURNAL OF INFECTION
(2015)
Article
Pediatrics
M. Elsa Villarino, Nigel A. Scott, Stephen E. Weis, Marc Weiner, Marcus B. Conde, Brenda Jones, Sharon Nachman, Ricardo Oliveira, Ruth N. Moro, Nong Shang, Stefan V. Goldberg, Timothy R. Sterling
Article
Infectious Diseases
Vincent Wing-Sang Cheng, Kenneth Siu-Sing Leung, Jamie Sui-Lam Kwok, Ross Ka-Kit Leung, Kevin Yi Yang, Raphael Chiu-Yeung Chan, Kai-Man Kam, Stephen Kwok-Wing Tsui
MICROBIAL DRUG RESISTANCE
(2016)
Review
Infectious Diseases
Celine Nadon, Ivo Van Walle, Peter Gerner-Smidt, Josefina Campos, Isabel Chinen, Jeniffer Concepcion-Acevedo, Brent Gilpin, Anthony M. Smith, Kai Man Kam, Enrique Perez, Eija Trees, Kristy Kubota, Johanna Takkinen, Eva Moller Nielsen, Heather Carleton
Article
Infectious Diseases
Tsz Ho Kwan, Shui Shan Lee, Denise Pui Chung Chan, Manton Cheung, Kai Man Kam
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2017)
Article
Multidisciplinary Sciences
Dachao Liang, Ross Ka Kit Leung, Shui Shan Lee, Kai Man Kam
Article
Immunology
T. K. Lam, H. Y. Lau, Y. P. Lee, S. M. Fung, W. L. Leung, K. M. Kam
INTERNATIONAL JOURNAL OF STD & AIDS
(2014)
Article
Immunology
Yung Chan, Kwok-Hung Yeung, Hing-Fung Ho, King-Man Ho, Edman Tin-Keung Lam, Wai-Lin Leung, Kai-Man Kam
INTERNATIONAL JOURNAL OF STD & AIDS
(2014)
Editorial Material
Infectious Diseases
Kai Man Kam
INTERNATIONAL JOURNAL OF TUBERCULOSIS AND LUNG DISEASE
(2014)
Article
Infectious Diseases
J. L. de Beer, C. Kodmon, J. van Ingen, P. Supply, D. van Soolingen
INTERNATIONAL JOURNAL OF TUBERCULOSIS AND LUNG DISEASE
(2014)
Article
Microbiology
Zhijian Zhang, Yufeng Wang, Yu Pang, Kai Man Kam
JOURNAL OF CLINICAL MICROBIOLOGY
(2014)
Letter
Environmental Sciences
Li Qiang, Dong Hai Yan, Pang Yu, Xia Hui, Ou Xi Chao, Zhang Zhi Ying, Li Jun Chen, Zhang Jian Kang, Huan Shi Tong, Chin Daniel P, Kam Kai Man, Zhao Yon Lin
BIOMEDICAL AND ENVIRONMENTAL SCIENCES
(2015)
Meeting Abstract
Economics
J. You, G. Lui, K. M. Kam, N. Lee