期刊
JOURNAL OF APPLIED MICROBIOLOGY
卷 106, 期 3, 页码 790-800出版社
WILEY
DOI: 10.1111/j.1365-2672.2008.04027.x
关键词
Biodegradation; biofilm; Burkholderia sp; NK8; chlorobenzoate; consortium; Pseudomonas aeruginosa PAO1
资金
- Ministry of Education, Culture, Sports, Science and Technology, Japan [19780242]
- Tokyo University of Agriculture [pAG408]
- Grants-in-Aid for Scientific Research [19780242] Funding Source: KAKEN
To characterize biofilm formation of a chlorobenzoates (CBs) degrading bacterium, Burkholderia sp. NK8, with another bacterial species, and the biodegradation activity against CBs in the mixed-species biofilm. Burkholderia sp. NK8 was solely or co-cultured with each of five other representative bacteria in microtitre dishes. Biofilm formation involving the strain NK8 was synergistically promoted by co-culturing with only Pseudomonas aeruginosa PAO1. Epifluorescent microscopy revealed that cells of the bacterial strain NK8 were viable and distributed randomly in the mixed-species biofilms. Enumeration of the attached cells on the surface of wells revealed that cells of the strain NK8 increased approx. 10-fold by the co-culture with the strain PAO1 compared to those by monoculture of the strain NK8, and the degradation activity of 3-chlorobenzoate by the dual-species biofilms was more promoted than that by the strain NK8-monocultured biofilms. Enhanced biofilm formation of Burkholderia sp. NK8 by the bacterial consortium occurred, but is determined by the partner bacterial species. The mixed-species biofilms have the advantage to degrade CBs on a solid surface. This study provides a significance of bacterial consortia on the biofilm formation and the degradation activity of Burkholderia sp. NK8, which contribute for complete degradation of chlorinated aromatics.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据