Reference genes in real-time PCR

How to perform RT-qPCR accurately in plant species? A case study on flower colour gene expression in an azalea (Rhododendron simsii hybrids) mapping population

(2013) Ellen De Keyser et al.   BMC MOLECULAR BIOLOGY

An improved qPCR protocol for rapid detection and quantification ofClostridium difficilein cattle feces

(2013) Petra Bandelj et al.   FEMS MICROBIOLOGY LETTERS

Evaluation of potential reference genes for use in gene expression studies in the conifer pathogen (Heterobasidion annosum)

(2013) Tommaso Raffaello et al.   MOLECULAR BIOLOGY REPORTS

Careful Selection of Reference Genes Is Required for Reliable Performance of RT-qPCR in Human Normal and Cancer Cell Lines

(2013) Francis Jacob et al.   PLoS One

Expression pattern of the psbO gene and its involvement in acclimation of the photosynthetic apparatus during abiotic stresses in Festuca arundinacea and F. pratensis

(2012) Izabela Pawłowicz et al.   ACTA PHYSIOLOGIAE PLANTARUM

Differential regulation of barley (Hordeum distichon) HVA1 and SRG6 transcript accumulation during the induction of soil and leaf water deficit

(2012) Magdalena Wójcik-Jagła et al.   ACTA PHYSIOLOGIAE PLANTARUM

Internal standards for quantitative RT-PCR studies of gene expression under drought treatment in barley (Hordeum vulgare L.): the effects of developmental stage and leaf age

(2012) Marcin Rapacz et al.   ACTA PHYSIOLOGIAE PLANTARUM

Evaluation of candidate reference genes for qPCR in maize

(2012) Alessandro Manoli et al.   JOURNAL OF PLANT PHYSIOLOGY

Selection and validation of reference genes for quantitative RT-PCR expression studies of the non-model crop Musa

(2012) Nancy Podevin et al.   MOLECULAR BREEDING

Evaluation of New Reference Genes in Papaya for Accurate Transcript Normalization under Different Experimental Conditions

(2012) Xiaoyang Zhu et al.   PLoS One

18S rRNA is a reliable normalisation gene for real time PCR based on influenza virus infected cells

(2012) Suresh V Kuchipudi et al.   Virology Journal

Standardisation and reporting for nucleic acid quantification

(2011) Jim Huggett et al.   ACCREDITATION AND QUALITY ASSURANCE

The effects of cold, light and time of day during low-temperature shift on the expression of CBF6, FpCor14b and LOS2 in Festuca pratensis

(2011) Barbara Jurczyk et al.   PLANT SCIENCE

An alternatively spliced domain of the NDC1 NAD(P)H dehydrogenase gene strongly influences the expression of the ACTIN2 reference gene in Arabidopsis thaliana

(2011) Sabá V. Wallström et al.   PLANT SCIENCE

Selection and validation of reference genes for real-time RT-PCR studies in the non-model species Delomys sublineatus, an endemic Brazilian rodent

(2010) Alexandra Weyrich et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Selection of optimal reference genes for normalization in quantitative RT-PCR

(2010) Inna Chervoneva et al.   BMC BIOINFORMATICS

A sequence-based approach to identify reference genes for gene expression analysis

(2010) Raj Chari et al.   BMC Medical Genomics

Validation of reference genes for quantitative RT-PCR studies of gene expression in perennial ryegrass (Lolium perenne L.)

(2010) Julia M Lee et al.   BMC MOLECULAR BIOLOGY

Validation of endogenous reference genes for qRT-PCR analysis of human visceral adipose samples

(2010) Rohini Mehta et al.   BMC MOLECULAR BIOLOGY

Reference gene selection for quantitative reverse transcription-polymerase chain reaction normalization during in vitro adventitious rooting in Eucalyptus globulus Labill

(2010) Márcia R de Almeida et al.   BMC MOLECULAR BIOLOGY

MIQE précis: Practical implementation of minimum standard guidelines for fluorescence-based quantitative real-time PCR experiments

(2010) Stephen A Bustin et al.   BMC MOLECULAR BIOLOGY

Validation of reference genes as internal control for studying viral infections in cereals by quantitative real-time RT-PCR

(2010) Jana Jarošová et al.   BMC PLANT BIOLOGY

Selection of reference genes for quantitative gene expression normalization in flax (Linum usitatissimum L.)

(2010) Rudy Huis et al.   BMC PLANT BIOLOGY

Validation of reference genes for quantitative real-time PCR during leaf and flower development in Petunia hybrida

(2010) Izaskun Mallona et al.   BMC PLANT BIOLOGY

A practical approach to RT-qPCR—Publishing data that conform to the MIQE guidelines

(2010) Sean Taylor et al.   METHODS

Accurate and objective copy number profiling using real-time quantitative PCR

(2010) Barbara D’haene et al.   METHODS

Reference gene selection for qPCR analysis during somatic embryogenesis in longan tree

(2010) Y.L. Lin et al.   PLANT SCIENCE

Reference gene selection for quantitative real-time PCR normalization in tomato subjected to nitrogen, cold, and light stress

(2009) Trond Løvdal et al.   ANALYTICAL BIOCHEMISTRY

Selection of appropriate reference genes for gene expression studies by quantitative real-time polymerase chain reaction in cucumber

(2009) Hongjian Wan et al.   ANALYTICAL BIOCHEMISTRY

A decade of improvements in quantification of gene expression and internal standard selection

(2009) Olivier Thellin et al.   BIOTECHNOLOGY ADVANCES

Identification and validation of reference genes for quantitative RT-PCR normalization in wheat

(2009) Anna R Paolacci et al.   BMC MOLECULAR BIOLOGY

Validation of reference genes for quantitative expression analysis by real-time RT-PCR in Saccharomyces cerevisiae

(2009) Marie-Ange Teste et al.   BMC MOLECULAR BIOLOGY

Identification of suitable internal control genes for expression studies in Coffea arabica under different experimental conditions

(2009) Carla F Barsalobres-Cavallari et al.   BMC MOLECULAR BIOLOGY

Selection of reliable reference genes for gene expression studies in peach using real-time PCR

(2009) Zhaoguo Tong et al.   BMC MOLECULAR BIOLOGY

Selection of reference genes for quantitative real-time PCR expression studies in the apomictic and sexual grass Brachiaria brizantha

(2009) Érica Silveira et al.   BMC PLANT BIOLOGY

The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments

(2009) S. A. Bustin et al.   CLINICAL CHEMISTRY

Different Patterns of Physiological and Molecular Response to Drought in Seedlings of Malt- and Feed-type Barleys (Hordeum vulgare)

(2009) M. Rapacz et al.   JOURNAL OF AGRONOMY AND CROP SCIENCE

Normalization of qRT-PCR data: the necessity of adopting a systematic, experimental conditions-specific, validation of references

(2009) S. Guenin et al.   JOURNAL OF EXPERIMENTAL BOTANY

How to do successful gene expression analysis using real-time PCR

(2009) Stefaan Derveaux et al.   METHODS

Why the need for qPCR publication guidelines?—The case for MIQE

(2009) Stephen A. Bustin   METHODS

Evaluation of coffee reference genes for relative expression studies by quantitative real-time RT-PCR

(2009) Fernanda Cruz et al.   MOLECULAR BREEDING

Validation of Candidate Reference Genes for the Accurate Normalization of Real-Time Quantitative RT-PCR Data in Rice During Seed Development

(2009) Qian-Feng Li et al.   PLANT MOLECULAR BIOLOGY REPORTER

The Effects of Cold Acclimation on Photosynthetic Apparatus and the Expression of COR14b in Four Genotypes of Barley (Hordeum vulgare) Contrasting in their Tolerance to Freezing and High-light Treatment in Cold Conditions

(2008) Marcin Rapacz et al.   ANNALS OF BOTANY

Instability of standard PCR reference genes in adipose-derived stem cells during propagation, differentiation and hypoxic exposure

(2008) Trine Fink et al.   BMC MOLECULAR BIOLOGY

RTPrimerDB: the portal for real-time PCR primers and probes

(2008) S. Lefever et al.   NUCLEIC ACIDS RESEARCH

The lack of a systematic validation of reference genes: a serious pitfall undervalued in reverse transcription-polymerase chain reaction (RT-PCR) analysis in plants

(2008) Laurent Gutierrez et al.   PLANT BIOTECHNOLOGY JOURNAL

Towards a Systematic Validation of References in Real-Time RT-PCR

(2008) L. Gutierrez et al.   PLANT CELL

Evaluation of reference genes for quantitative RT-PCR in Lolium temulentum under abiotic stress

(2008) James E. Dombrowski et al.   PLANT SCIENCE