期刊
JOURNAL OF APPLIED GENETICS
卷 52, 期 2, 页码 125-136出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s13353-010-0001-5
关键词
Anti-HBV oral vaccine; Lyophilized plant tissue; Oral immunization; Plant-based vaccines; S-HBsAg; Transgenic lettuce
资金
- Polish State Committee for Scientific Research [2 P04B 001 27, N401-O/0026/32]
- Medana Pharma Ltd., Sieradz, Poland
Efficient immunization against hepatitis B virus (HBV) and other pathogens with plant-based oral vaccines requires appropriate plant expressors and the optimization of vaccine compositions and administration protocols. Previous immunization studies were mainly based on a combination of the injection of a small surface antigen of HBV (S-HBsAg) and the feeding with raw tissue containing the antigen, supplemented with an adjuvant, and coming from plants conferring resistance to kanamycin. The objective of this study was to develop a prototype oral vaccine formula suitable for human immunization. Herbicide-resistant lettuce was engineered, stably expressing through progeny generation micrograms of S-HBsAg per g of fresh weight and formed into virus-like particles (VLPs). Lyophilized tissue containing a relatively low, 100-ng VLP-assembled antigen dose, administered only orally to mice with a long, 60-day interval between prime and boost immunizations and without exogenous adjuvant, elicited mucosal and systemic humoral anti-HBs responses at the nominally protective level. Lyophilized tissue was converted into tablets, which preserved S-HBsAg content for at least one year of room temperature storage. The results of the study provide indications on immunization methodology using a durable, efficacious, and convenient plant-derived prototype oral vaccine against hepatitis B.
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