期刊
JOURNAL OF APPLIED GENETICS
卷 50, 期 2, 页码 77-82出版社
SPRINGER HEIDELBERG
DOI: 10.1007/BF03195657
关键词
BAC-FISH; chromosome measurements; idiogram; Lupinus angustifolius; resistance genes
资金
- State Committee for Scientific Research, Warsaw, Poland [3 P06A 009 24]
BAC (bacterial artificial chromosome) clones from the genomic BAC library of the narrow-leafed lupin (Lupinus angustifolius) were used for cytogenetic mapping of mitotic metaphase chromosomes of that species by the BAC-FISH technique. Location of the clones, together with cytogenetic markers localised earlier by FISH (fluorescence in situ hybridisation) and PRINS (primed in situ DNA labelling), was combined with computer-aided chromosome measurements, to construct the first idiogram of the narrow-leafed lupin. The chromosomes are meta- or submetacentric; the mean absolute chromosome lengths range from 1.9 mu m to 3.8 mu m, and mean relative lengths from 1.6% to 3.3%. Data concerning linkage of resistance to 2 fungal pathogens as well as assignment of the second linkage group to the appropriate chromosome are given for the first time.
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