Review
Microbiology
Burcu Isler, Yukiko Ezure, Jose Luis Garcia-Fogeda Romero, Patrick Harris, Adam G. Stewart, David L. Paterson
Summary: Ceftazidime/avibactam may be a carbapenem-sparing option for the treatment of mild to moderate complicated urinary tract and intra-abdominal infections caused by ESBL-producing Enterobacterales species, while the data are too limited to provide any conclusive recommendations for the AmpC producers. Care should be taken before extrapolating this to severe infections, given that the representation of this population in the reviewed studies was negligible.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Microbiology
Mohamed A. Nossair, Fatma A. Abd El Baqy, Mohammad S. Y. Rizk, Haitham Elaadli, Alaa M. Mansour, Ayman H. Abd El-Aziz, Adil Alkhedaide, Mohamed Mohamed Soliman, Hazem Ramadan, Mustafa Shukry, Sabah Shaaban
Summary: A cross-sectional study was conducted in dairy cattle farms and poultry farms in northern Egypt to assess the prevalence of ESBL-producing Enterobacteriaceae in humans, cattle, and poultry. The study found that the prevalence of ESBL-producing Enterobacteriaceae was 30.0%, 20.0%, and 25.0% in humans, cattle, and poultry, respectively. The majority of the ESBL-producing Enterobacteriaceae showed a multi-drug resistant phenotype.
Article
Infectious Diseases
Paolo Gaibani, Federica Bovo, Linda Bussini, Tiziana Lazzarotto, Stefano Amadesi, Michele Bartoletti, Pierlugi Viale, Simone Ambretti
Summary: In this study, the in vivo evolution of resistance to imipenem/cilastatin/relebactam in Klebsiella pneumoniae carbapenemase (KPC)-producing Klebsiella pneumoniae (KPC-Kp) strains from a patient treated with different ceftazidime/avibactam-based therapies was described. The rapid development of mutations and adaptability of the KPC-Kp genome highlight the potential threat of these bacteria.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2022)
Article
Infectious Diseases
Isabella A. Tickler, Diane Kawa, Anne E. Obradovich, Ferric C. Fang, Fred C. Tenover
Summary: A total of 199 Gram-negative bacterial isolates from urinary tract infections and 162 from bloodstream infections were collected from 12 healthcare systems throughout the United States between May 2021 and August 2022. The study characterized the isolates that were resistant to cephalosporins or carbapenems through antimicrobial susceptibility testing and whole genome sequencing. The results showed a wide range of carbapenemase-mediated resistance, with blaKPC-3 and blaKPC-2 being the most common carbapenemase genes identified. Additionally, extended-spectrum beta-lactamase genes and AmpC genes were also detected in a significant number of isolates. Importantly, some of the carbapenemase-producing organisms were still susceptible to certain antibiotics.
Article
Multidisciplinary Sciences
Fangzhou Wang, Qian Zhou, Xiuwen Yang, Yan Bai, Junchang Cui
Summary: The study found that ceftazidime/avibactam exhibited good susceptibility against KPC-Kp, and combination therapy with amikacin and tigecycline showed better antibacterial effects. Monotherapy may be inadequate in managing KPC-Kp infections, highlighting the potential benefits of combination therapy.
Article
Microbiology
Francisco Sanz Herrero
Summary: The combination therapy of ceftazidime-avibactam offers an interesting therapeutic option for nosocomial pneumonia caused by beta-lactamase-producing Gram-negative bacilli. It has shown non-inferiority compared to carbapenems, with better cure rates and mortality compared to colistin. The limitation of ceftazidime-avibactam in treating infections caused by metallo-beta-lactamase-producing Enterobacteriaceae can be overcome by adding aztreonam.
REVISTA ESPANOLA DE QUIMIOTERAPIA
(2022)
Article
Infectious Diseases
Ananda Tiwari, Jaana Paakkanen, Ahmad I. Al-Mustapha, Juha Kirveskari, Rene S. Hendriksen, Annamari Heikinheimo
Summary: Analyzing samples of municipal wastewater influent can help to map the status of antibiotic-resistant bacteria (ARB) and predict the public health risks. In this study, bacterial isolates carrying beta-lactamase genes in wastewater were investigated and their characteristics were compared.
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2023)
Article
Microbiology
Xueting Wang, Luying Xiong, Yuan Wang, Kai Yang, Tingting Xiao, Xiaohui Chi, Tao Chen, Yanzi Zhou, Ping Lu, Dilimulati Dilinuer, Pin Shen, Yunbo Chen, Yonghong Xiao
Summary: This study evaluated the effect of inoculum size on the in vitro antibacterial effects of imipenem/relebactam (IMR) and ceftazidime/avibactam (CZA) against ESBL-producing-, AmpC-producing-, and KPC-producing Escherichia coli and Klebsiella pneumoniae. The results showed that an increase in inoculum size could affect the antibacterial effects of both IMR and CZA, especially for SHV-producing and KPC-producing strains.
ANNALS OF CLINICAL MICROBIOLOGY AND ANTIMICROBIALS
(2023)
Article
Microbiology
Zeyu Huang, Haifeng Liu, Xiaotuan Zhang, Miran Tang, Yuzhan Lin, Luozhu Feng, Jianzhong Ye, Tieli Zhou, Lijiang Chen
Summary: Nanoparticle-based antibiotic delivery systems, such as ceftazidime-decorated gold nanoparticles (CAZ_Au NPs), effectively kill ceftazidime-avibactam-resistant Enterobacteriaceae with various resistance mechanisms and inhibit biofilm formation. CAZ_Au NPs damage bacterial cell membrane, increase intracellular reactive oxygen species, and exhibit no significant toxicity at bactericidal concentrations. Furthermore, they improve survival rate in a mouse model of abdominal infection, making them a promising approach to enhance the potency of ceftazidime as an antibiotic.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2023)
Article
Biochemistry & Molecular Biology
Forough Babazadeh, Roghayeh Teimourpour, Mohsen Arzanlou, Hadi Peeridogaheh, Mehdi Yousefipour, Jafar MohammadShahi
Summary: The objective of this study was to evaluate the phenotypic and molecular characterization of ESBL/AmpC- and carbapenemase-producing K. pneumoniae isolates in Iran. The results showed that K. pneumoniae isolates had high resistance rates to cefazolin and cefotaxime, while meropenem and amikacin exhibited high sensitivity rates. The most frequent carbapenemase-encoding genes were bla(OXA-48) and bla(IMP), while bla(KPC) and bla(GTM) were not detected. Among the ESBL and AmpC beta-lactamase-encoding genes, bla(TEM) had the highest frequency.
MOLECULAR BIOLOGY REPORTS
(2022)
Article
Infectious Diseases
Melese Hailu Legese, Daniel Asrat, Abraham Aseffa, Badrul Hasan, Adane Mihret, Gote Swedberg
Summary: This study aimed to characterize ESBL and AmpC producing Enterobacteriaceae from sepsis patients. The results showed high genotypic frequencies of ESBL producing Enterobacteriaceae, with Hawassa having the highest detection rate. Several variants of ESBL and AmpC genes were detected.
Article
Microbiology
Lynn-Yao Lin, Dmitri Debabov, William Chang, Gregory Stone, Todd Riccobene
Summary: This study evaluated the in vitro activities and clinical outcomes of Ceftazidime-Avibactam (CAZ-AVI) against different drug-resistant strains, comparing it to other antimicrobial agents. The results showed that CAZ-AVI had good in vitro activity and clinical efficacy against AmpC-overproducing Enterobacterales and Pseudomonas aeruginosa, especially in OXA-48-producing Enterobacterales.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Infectious Diseases
Min Xu, Jun Zhao, Li Xu, Qing Yang, Hao Xu, Haishen Kong, Jianying Zhou, Yiqi Fu
Summary: This study identified conjugative plasmid-mediated bla(CMY-172) as a new mechanism for CAZ/AVI resistance in clinical KPC-Kp strains. Careful monitoring of CAZ/AVI susceptibility is crucial to prevent the spread of the resistance gene.
CLINICAL MICROBIOLOGY AND INFECTION
(2022)
Article
Infectious Diseases
Hakeam A. Hakeam, Hala Alsahli, Lama Albabtain, Shahad Alassaf, Zainab Al Duhailib, Sahar Althawadi
Summary: In patients with CRE bacteremia, ceftazidime-avibactam was associated with a lower risk of 14-day mortality compared to colistin, and had a higher clinical success rate.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2021)
Article
Microbiology
Luozhu Feng, Yining Zhao, Zhuocheng Yao, Xiaodong Zhang, Shufang Zheng, Lijiang Chen, Ying Zhang, Lingbo Wang, Tieli Zhou, Jianming Cao
Summary: This study developed a rapid ResaCeftazidime-avibactam Enterobacterales NP test, which can detect the susceptibility of Enterobacterales to CZA within a short period of time with high accuracy and consistency. This method is economical and convenient, and may be used for the rapid screening and timely treatment of CZA-resistant strains in clinical settings.
JOURNAL OF CLINICAL MICROBIOLOGY
(2022)
Article
Infectious Diseases
Simon Packers, Bruno Pichon, Stephen Thompson, Jane Neale, Jacquelyn Njoroge, Rachel M. Kwiatkowska, Isabel Oliver, Maggie Telfer, Michel Doumith, Camillus Buunaaisie, Ellen Heinsbroek, Noreen Hopewell-Kelly, Monica Desai, Vivian Hope, Owen Martin Williams, Angela Kearns, Mathew Hickman, Maya Gobin
Article
Infectious Diseases
Anais Painset, Martin Day, Michel Doumith, Jonathan Rigby, Claire Jenkins, Kathie Grant, Timothy J. Dallman, Gauri Godbole, Craig Swift
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2020)
Article
Public, Environmental & Occupational Health
Majed F. Alghoribi, Khalifa Binkhamis, Abdulrahman A. Alswaji, Ali Alhijji, Aynaa Alsharidi, Hanan H. Balkhy, Michel Doumith, Ali Somily
JOURNAL OF INFECTION AND PUBLIC HEALTH
(2020)
Article
Genetics & Heredity
Cheryll M. Sia, David R. Greig, Martin Day, Hassan Hartman, Anais Painset, Michel Doumith, Daniele Meunier, Claire Jenkins, Marie Anne Chattaway, Katie L. Hopkins, Neil Woodford, Gauri Godbole, Timothy J. Dallman
MICROBIAL GENOMICS
(2020)
Letter
Infectious Diseases
Majed F. Alghoribi, Taseen S. Desin, Abdulrahman A. Alswaji, Bassam H. Al Alwan, Maha Alzayer, Liliane Okdah, Sameera Al Johani, Hanan H. Balkhy, Michel Doumith
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2020)
Article
Infectious Diseases
Shazad Mushtaq, Anna Vickers, Michel Doumith, Matthew J. Ellington, Neil Woodford, David M. Livermore
Summary: Taniborbactam potentiates partner beta-Lactams against various Enterobacterales with different carbapenemases, showing potential broader spectrum compared to vaborbactam.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2021)
Article
Multidisciplinary Sciences
Majed F. Alghoribi, Moayad Alqurashi, Liliane Okdah, Bassam Alalwan, Yahya S. AlHebaishi, Abdulmajeed Almalki, Maha A. Alzayer, Abdulrahman A. Alswaji, Michel Doumith, Mazin Barry
Summary: The challenging Pandrug-resistant K. pneumoniae infection can be successfully overcome and cleared rapidly through testing different drug combinations.
SCIENTIFIC REPORTS
(2021)
Article
Microbiology
Michel Doumith, Sarah Alhassinah, Abdulrahman Alswaji, Maha Alzayer, Essa Alrashidi, Liliane Okdah, Sameera Aljohani, Hanan H. Balkhy, Majed F. Alghoribi
Summary: Carbapenem-resistant P. aeruginosa is a major clinical problem in Saudi Arabia. This study provides insights into the genetic characteristics and antibiotic resistance mechanisms of these clinical isolates, highlighting the global spread of specific sub-lineages. It emphasizes the importance of routine detection of rare beta-lactamases.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Medicine, Research & Experimental
Maha Alamri, Heba Alghamdi, Sahar Althawadi, Maysoon Mutabaggani, M. Anas Dababo, Fahad Alajlan, Maha Alzayer, Michel Doumith, Majed Alghoribi, Reem S. Almaghrabi
Summary: Neoscytalidium is a plant pathogen commonly found in plants and soil, causing skin and nail infections as well as invasive diseases in immunocompromised patients. Despite combined surgical and medical therapies, treating invasive fungal diseases caused by Neoscytalidium can be challenging and often result in fatal outcomes.
MEDICAL MYCOLOGY CASE REPORTS
(2021)
Article
Microbiology
Katie L. Hopkins, Nicholas Ellaby, Matthew J. Ellington, Michel Doumith, Nazim Mustafa, Daniele Meunier, Neil Woodford
Summary: By sequencing CPE samples from the national reference laboratory in England, we found that the epidemiology of CPE in England is diverse, indicating multiple introductions and a role for horizontal transfer of carbapenemase genes.
JOURNAL OF MEDICAL MICROBIOLOGY
(2022)
Article
Infectious Diseases
Maha Alzayer, Majed F. Alghoribi, Bassam Alalwan, Abdualah Alreheli, Sameera Aljohani, Mohammad Bosaeed, Michel Doumith
Summary: This study investigated the in vitro activity of Cefiderocol against carbapenem non-susceptible Gram-negative bacteria collected in Saudi Arabia. The results showed that Cefiderocol exhibited excellent activity, especially against multidrug-resistant non-fermenting Gram-negative pathogens.
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2023)
Article
Infectious Diseases
Maha Alzayer, Manal M. Alkhulaifi, Ahmed Alyami, Mohammed Aldosary, Abdulaziz Alageel, Ghada Garaween, Atef Shibl, Arif M. Al-Hamad, Michel Doumith
Summary: Group B Streptococcus (GBS) has emerged as an important cause of severe infections in adults. This study investigated the epidemiology of GBS in Saudi Arabia and found that serotypes III and V were the most prevalent, and most isolates harbored pilus island 1 and 2a types. The isolates exhibited high levels of resistance to erythromycin and clindamycin, which require further surveillance.
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2023)
Article
Infectious Diseases
Antoine Abou Fayad, Maria El Azzi, Ahmad Sleiman, Issmat I. Kassem, Reema A. Bawazeer, Liliane Okdah, Michel Doumith, Majed F. Alghoribi, Ghassan M. Matar
Summary: The study revealed the presence of mcr and other important resistance determinants in MDR E. coli isolated from poultry farms in Lebanon, with resistance genes predominantly located on IncX4 plasmids. Some strains may pose a risk of transmission to humans.
JAC-ANTIMICROBIAL RESISTANCE
(2021)
Article
Microbiology
Majed F. Alghoribi, Michel Doumith, Mathew Upton, Sameera M. Al Johani, Maha Alzayer, Neil Woodford, Matthew J. Ellington, Hanan H. Balkhy
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2019)
Article
Infectious Diseases
David M. Livermore, Michaela Day, Paul Cleary, Katie L. Hopkins, Mark A. Toleman, David W. Wareham, Camilla Wiuff, Michel Doumith, Neil Woodford
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2019)