Article
Immunology
Zhen-Yu Wang, Yue Jiang, Yi-Qiao Shao, Heng-Fan Lu, Meng-Jun Lu, Xinan Jiao, Jing Wang, Qiu-Chun Li
Summary: This study investigates the prevalence and dissemination mechanism of ESBL-producing E. coli from healthy individuals in a community in Yangzhou, China. The study found nasal colonization of CTX-M-55-producing E. coli strains among healthy individuals in the community, indicating a potential risk of antimicrobial resistance dissemination through close contact or environmental transmission.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Microbiology
Shihan Zeng, Jiajun Luo, Xiaoyan Li, Chao Zhuo, Aiwu Wu, Xiankai Chen, LiShao Huang
Summary: The CTX-M-55 extended-spectrum beta-lactamase (ESBL)-positive rate has been increasing in recent years, showing resistance to ceftriaxone but sensitivity to ceftazidime, cefepime, and carbapenems among isolates with bla(CTX-M-55) gene. ST1193 is a common clone strain found carrying the bla(CTX-M-55) gene. The plasmids IncI1 and IncFII, along with mobile elements like ISEcp1 and IS26, may play a key role in the dissemination of CTX-M-55 genotypes.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Xiaojie Qin, Zengfeng Zhang
Summary: The increasing resistance to cephalosporins in Salmonella is a serious threat to public health. A new bla(CTX-M) variant, bla(CTX-M-101), was first reported in Salmonella enterica serovar Enteritidis. Further analysis of isolate SJTUF14523 carrying bla(CTX-M-101) revealed its genome characterization, transferability, and resistance mechanism. This multidrug resistance isolate exhibited high resistance to ceftazidime, cefotaxime, and cefepime. The presence of plasmid p14523A resulted in increased resistance to cephalosporins in Escherichia coli C600. Gene cloning and plasmid sequencing showed that bla(CTX-M-101) was located on a transferable plasmid, p14523A, and a composite transposon unit was found in this plasmid. These findings emphasize the challenges posed by new CTX-M-101-like variants in Salmonella and the need for prevention and control of antibiotic resistance.
Article
Public, Environmental & Occupational Health
Misheck Shawa, Yoshikazu Furuta, Gillan Mulenga, Maron Mubanga, Evans Mulenga, Tuvshinzaya Zorigt, Christone Kaile, Manyando Simbotwe, Atmika Paudel, Bernard Hang'ombe, Hideaki Higashi
Summary: The study revealed the co-occurrence of ISEcp1-bla(CTX-M-15) and multiple antimicrobial resistance genes on chromosomal insertions in Enterobacter cloacae and Escherichia coli, potentially facilitating the propagation of multidrug-resistant clones. The chromosomal integration of bla(CTX-M) in certain strains may play a role in the dissemination of antimicrobial resistance genes compared to the plasmid location.
ANTIMICROBIAL RESISTANCE AND INFECTION CONTROL
(2021)
Article
Infectious Diseases
Amel Mhaya, Rahma Trabelsi, Sabine Aillerie, Fatima M'Zali, Dominique Begu, Slim Tounsi, Radhouane Gdoura, Corinne Arpin
Summary: Multiple ESBL-producing strains were identified in Tunisian patients, including two emerging virulent clones, ST131-C2 and ST131-C1-M27, which were found to be prevalent in both community and hospital settings.
Article
Microbiology
Owen Higgins, Alexandra Chueiri, Louise O'Connor, Sinead Lahiff, Liam Burke, Dearbhaile Morris, Nicola Maria Pfeifer, Belen Gonzalez Santamarina, Christian Berens, Christian Menge, Manuela Canica, Vera Manageiro, Veljo Kisand, Marwa M. M. Hassan, Brian Gardner, Arnoud H. M. van Vliet, Roberto M. La Ragione, Bruno Gonzalez-Zorn, Terry J. J. Smith
Summary: CTX-M ESBL-producing E. coli is an increasing public health concern, and accurate identification of CTX-M variants is crucial for disease monitoring and treatment. CTX-M-1 and CTX-M-15 are the main ESBL-producing Enterobacteriaceae associated with animal and human infections.
MICROBIOLOGY SPECTRUM
(2023)
Article
Medicine, Research & Experimental
Christopher O. Fakorede, Kehinde O. Amisu, Morteza Saki, Kabiru O. Akinyemi
Summary: This study investigated antibiotic resistance and the carriage of bla(CTX-M-9) and bla(CTX-M-15) among Salmonella species from hospitalised patients in Lagos, Nigeria. It found a high level of resistance to cephalosporins, penicillin, and some carbapenems, indicating the need for prudent use of cephalosporins.
EUROPEAN JOURNAL OF MEDICAL RESEARCH
(2023)
Article
Microbiology
Linda Falgenhauer, Anja Zur Nieden, Susanne Harpel, Jane Falgenhauer, Eugen Domann
Summary: The presence of ESBL-producing E. coli in surface water, closely related to human clinical isolates, suggests a potential transmission pathway. This highlights the underreported role of surface water in the spread of ESBL-producing isolates.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Immunology
Fikri S. Widyatama, Nobuyoshi Yagi, Rosantia Sarassari, Toshiro Shirakawa, Danh Tuyen Le, Mai Huong Thi Bui, Kuntaman Kuntaman, Itaru Hirai
Summary: ESBL-producing Escherichia coli have been detected in healthy individuals in Indonesia and Vietnam, with different upstream genetic structures (UGS) suggesting separate distribution of plasmids harboring bla(CTX-M) in the two countries.
MICROBIOLOGY AND IMMUNOLOGY
(2021)
Article
Infectious Diseases
L. Saiz-Escobedo, I. Cadenas-Jimenez, R. Olmos, A. Carrera-Salinas, D. Berbel, J. Camara, F. Tubau, M. A. Dominguez, C. Ardanuy, A. Gonzalez-Diaz, S. Marti
Summary: Four cephalosporin-resistant H. parainfluenzae strains from patients with urethritis were found to be multidrug-resistant, and a novel ESBL resistance gene was identified. The transmission potential of these resistance genes to other pathogens is a cause for concern.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2023)
Article
Microbiology
Eva A. Edward, Nelly M. Mohamed, Azza S. Zakaria
Summary: Egypt has recently experienced the emergence of multidrug-resistant Klebsiella pneumoniae, presenting a significant challenge to healthcare. The rapid dissemination of bla(CTX-M) genes, particularly bla(CTX-M-14) and bla(CTX-M-15), among these multidrug-resistant K. pneumoniae has been observed. This study investigates the occurrence of bla(CTX-M-IV) among K. pneumoniae isolates from a major hospital in Alexandria, Egypt. Approximately 22% of the tested isolates were found to carry the bla(CTX-M-IV) gene. Whole genome sequencing revealed the presence of high-risk clone ST383 in one of the isolates, which carried bla(CTX-M-14) on an IncL/M plasmid and bla(CTX-M-15) on a hybrid IncHI1B/IncFIB plasmid. These plasmids showed similarities to those found in other countries such as the UK, Germany, and China. The presence of plasmids with enhanced genetic plasticity poses a significant threat as it allows the evolution of K. pneumoniae isolates causing untreatable infections. Nationwide surveillance is urgently needed to monitor the prevalence of these superbugs with limited treatment options.
Article
Fisheries
S. Ranjani, Pradeep Parthasarathy, P. Rameshkumar, U. VimalKumar, S. Hemalatha
Summary: The study successfully controlled the proliferation of bacterial pathogens causing vibriosis by synthesizing pungent acrid silver nanocolloids, which are cost-effective and can be used as an alternative to prevent disease outbreaks.
AQUACULTURE INTERNATIONAL
(2021)
Article
Genetics & Heredity
Milan S. Stosic, Marianne Sunde, Solveig Solverod Mo, Amar Anandrao Telke, Knut Rudi
Summary: The study found that in IncI1 plasmids, the presence of the extended-spectrum cephalosporin resistance gene bla(CTX-M-1) affects the rearrangement of the shufflon region, resulting in interrupted shufflons producing fewer variants with segment-loss frequency being distinctive in different plasmid hosts. While it is hypothesized that the A-segment plays a significant role during conjugation, further research is needed to confirm its importance.
Article
Microbiology
Ke Ma, Zhiyong Zong
Summary: This study investigated the mechanism of aztreonam-avibactam resistance in a carbapenem-resistant clinical strain of Escherichia coli. The results showed that E. coli strains with the YRIK insertion in PBP3, and producing CTX-M-15 or CTX-M-199 enzymes, were resistant to aztreonam-avibactam.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Parvaneh Ahmadvand, Johannetsy J. Avillan, Jacob A. Lewis, Douglas R. Call, ChulHee Kang
Summary: This study determined the crystal structures of CTX-M-15 in complex with different compounds and provided insights into its catalytic and binding mechanism. The affinity of CTX-M-15 for beta-lactam compounds was found to be similar, but lower for clavulanic acid. The presence of amine and furfural moieties in compounds could hinder the hydrolytic activity of CTX-M-15.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Letter
Infectious Diseases
Yun Young Cho, So Yeon Kim, Kwan Soo Ko
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2021)
Article
Infectious Diseases
Jee Hong Kim, Yun Young Cho, Ji Young Choi, Yu Mi Wi, Kwan Soo Ko
Summary: This study investigated the characteristics of KPC-2-producing Klebsiella pneumoniae isolates from a hospital in South Korea. It identified two main clones, ST11 and ST307, with different resistance profiles and virulence levels. Continuous monitoring of these clones, which show different characteristics including resistance and virulence, is necessary.
Retraction
Microbiology
Juyoun Shin, Kwan Soo Ko
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Microbiology
Yun Young Cho, Jee Hong Kim, Hyunkeun Kim, Junghwa Lee, Se Jin Im, Kwan Soo Ko
Summary: This study investigated the characteristics of two main clones of carbapenemase-producing Klebsiella pneumoniae isolates, ST11 and ST307, from South Korea. The findings showed that ST11 isolates had a wide range of characteristics, including serotype and virulence, compared to the more homogeneous ST307 isolates. The wzi14-type among the ST11 isolates exhibited higher virulence, while the ST307 isolates had similar virulence to the wzi14-type ST11 isolates. Therefore, monitoring the detailed serotype is necessary in addition to tracking the introduction and spread of specific clones.
Retraction
Microbiology
So Yeon Kim, Ji-Young Rhee, Kwan Soo Ko
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2023)
Retraction
Microbiology
Juyoun Shin, Jin Yang Baek, Sun Young Cho, Hee Jae Huh, Nam Yong Lee, Jae-Hoon Song, Doo Ryeon Chung, Kwan Soo Ko
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2023)
Article
Microbiology
Hyeri Seok, Ji-Young Choi, Michidmaral Ganbold, Jong Hyun Shin, Jooyun Kim, Won Suk Choi, Dae Won Park, Kwan Soo Ko
Summary: Recently, there has been an increase in the number of reported infections caused by non-diphtheriae Corynebacterium spp. in patients, as well as the discovery of novel species of Corynebacterium isolated from humans. We report two cases of human infections caused by Corynebacterium haemomassiliense-like organisms, which were initially unidentified by MALDI-TOF MS analysis. Further analysis using three housekeeping genes and phenotypic features revealed their close relation to the recently described species C. haemomassiliense. These strains were found to be multidrug-resistant but susceptible to vancomycin, meropenem, and linezolid. Our report suggests that these recently described Corynebacterium species may not be limited to a specific region, highlighting the difficulty in classifying the genus Corynebacterium.
CURRENT MICROBIOLOGY
(2023)
Article
Infectious Diseases
Jeongwoo Jo, Wonbeom Park, Dae-Hyuk Kweon, Kwan Soo Ko
Summary: Tigecycline heteroresistance is highly prevalent in Acinetobacter baumannii clinical isolates, reducing the efficacy of tigecycline treatment. This study confirmed the reproducibility and stability of mimicked tigecycline heteroresistance and found that the development of resistance in tigecycline-heteroresistant A. baumannii strains may depend on the selection of a pre-existing resistant subpopulation.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2023)
Article
Microbiology
Hyunkeun Kim, Jee Hong Kim, Hongbaek Cho, Kwan Soo Ko
Summary: This study reveals that DNA methyltransferase may regulate persister cell formation in Acinetobacter, while RecC seems to mediate epigenetic regulation of persister cell formation. These findings uncover novel mechanisms of persister cell formation, which are important for the research on antibiotic treatment and antibiotic resistance.
MICROBIOLOGY SPECTRUM
(2022)
Article
Infectious Diseases
Seon-Yeong Kim, Hwi Won Seo, Min-Seon Park, Chul Min Park, Jinho Seo, Jaerang Rho, Subeen Myung, Kwan Soo Ko, Jun-Seob Kim, Choong-Min Ryu
Summary: Researchers have developed a chemical compound called PA108 that enhances the antimicrobial activity of polymyxin B against resistant bacteria, offering a potential solution to antibiotic resistance.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2023)
Article
Microbiology
Sun Ju Kim, Jeongwoo Jo, Kwan Soo Ko
Summary: This study found that colistin resistance in K. variicola was mediated by the modification of lipid A. Although the isolate was obtained from faecal samples of healthy adults, colistin-resistant K. variicola challenges public health as an opportunistic pathogen.
JOURNAL OF MEDICAL MICROBIOLOGY
(2023)
Article
Infectious Diseases
Yun Young Cho, Kwan Soo Ko
Summary: This study investigated the impact of plasmids bearing carbapenemase genes on the formation of persister cells. Results showed that the ColE-type plasmid with blaOXA-232 significantly increased the formation of persister cells. Transformants with gene deletions showed decreased persister cell formation compared to transformants harboring intact pM5_OXA232. The expression levels of relA and spoT correlated with persister cell formation rates, especially against ciprofloxacin. This study highlights the influence of a small ColE-type plasmid bearing blaOXA-232 on persister cell formation.
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2023)
Article
Infectious Diseases
Jeongwoo Jo, Ji Young Lee, Hongbaek Cho, Kwan Soo Ko
Summary: Recent studies have found that colistin dependence frequently occurs in colistin-susceptible Acinetobacter baumannii isolates. Although the parental strains are resistant, colistin-dependent mutants showed increased susceptibility to other antibiotics, suggesting the possibility of developing strategies to eliminate multidrug-resistant A. baumannii. Combination treatments of colistin with subinhibitory concentrations of other antibiotics, especially amikacin, inhibited the development of colistin dependence and eradicated the strains in in vitro assays.
MICROBIAL DRUG RESISTANCE
(2023)
Article
Cell Biology
Jeongwoo Jo, Ki Tae Kwon, Kwan Soo Ko
Summary: This study investigated the heteroresistance of Acinetobacter baumannii to tigecycline and colistin, and evaluated the effectiveness of combined antibiotic treatment. The findings showed that A. baumannii exhibited heteroresistance to both antibiotics, but the resistant subpopulations were independent from each other. Therefore, the combination of tigecycline and colistin can effectively eliminate this bacterial infection.
JOURNAL OF BIOMEDICAL SCIENCE
(2023)
Article
Cell Biology
Suyeon Park, Hyunkeun Kim, Kwan Soo Ko
Summary: In hypervirulent Klebsiella pneumoniae strains, tigecycline resistance is associated with decreased virulence due to reduced production of capsular polysaccharides (CPS). Transcriptomic analyses revealed downregulation of ompK35 expression in tigecycline-resistant mutants, which overexpressed ompR and negatively regulated ompK35 expression. Tigecycline-resistant and ompR-overexpressed mutants exhibited reduced hypermucoviscosity and virulence, while deletion of ompR restored these phenotypes in tigecycline-resistant mutants.
JOURNAL OF BIOMEDICAL SCIENCE
(2023)