Article
Microbiology
Amin Khoshbayan, Rezvan Golmoradi Zadeh, Majid Taati Moghadam, Shiva Mirkalantari, Atieh Darbandi
Summary: This study found the importance and high prevalence of E. coli O25b/ST131 among UTI isolates, with the ability to rapidly spread and disseminate antibiotic resistance genes.
ANNALS OF CLINICAL MICROBIOLOGY AND ANTIMICROBIALS
(2022)
Article
Infectious Diseases
Shengcen Zhang, Qianwen Zhang, Jiangqing Huang, Yingping Cao, Zhichang Zhao, Bin Li
Summary: The pathogenicity and survivability features of E. coli O16:H41-ST131 were similar to the global epidemic O25b:H30-ST131 lineage, with differences observed in their anti-phagocytic function. This weaker anti-phagocytic function of E. coli O16:H41-ST131 could potentially contribute to its increasing prevalence.
INFECTION AND DRUG RESISTANCE
(2021)
Article
Infectious Diseases
Soha S. Abdelrahim, Magdy Fouad, Nilly Abdallah, Rasha F. Ahmed, Shaimaa Zaki
Summary: The study identified a high prevalence of the O25-ST131 clone among E. coli isolates, with significantly higher rates of ESBL, MDR, and antibiotic resistance. The presence of the CTX-M-15 gene was notably high in intestinal isolates, while the O16-ST131 clone was not detected in the study samples.
INFECTION AND DRUG RESISTANCE
(2021)
Article
Microbiology
Mario Galindo-Mendez, Humberto Navarrete-Salazar, Reinaldo Pacheco-Vasquez, Devanhi de la Paz, Isabel Baltazar-Jimenez, Jose David Santiago-Luna, Laura Guadarrama-Monroy
Summary: The study aimed to determine the prevalence of plasmid-mediated colistin resistance genes, mcr-1 to mcr-5, among colistin and multi-drug-resistant Gram-negative bacilli strains in a tertiary hospital in Toluca, Mexico. PCR tests were conducted to detect the presence of mcr genes among 241 collected strains. Conjugation experiments were carried out to assess the horizontal transmission of colistin resistance, confirming the presence of plasmid-mediated colistin resistance and the transmission of mcr genes in Mexican hospitalized patients.
Article
Medical Laboratory Technology
Yanfang Lu, Jiangqing Huang, Jiaqi Su, Huiyu Chen, Zhihui Wu, Boqiu Zhang
Summary: The study investigated the prevalence and molecular characteristics of E. coli ST131 and non-ST131 isolates that cause bloodstream infections, with ST131 isolates showing higher virulence factors, incidence of urinary tract infections, and hospital stay time. The dominant serotype was O25b-ST131.
CLINICAL LABORATORY
(2022)
Article
Immunology
Laurent Chorro, Zhenghui Li, Ling Chu, Suddham Singh, Jianxin Gu, Jin-hwan Kim, Kaushik Dutta, Rosalind Pan, Srinivas Kodali, Duston Ndreu, Axay Patel, Julio C. Hawkins, Chris Ponce, Natalie Silmon de Monerri, David Keeney, Arthur Illenberger, C. Hal Jones, Lubomira Andrew, Jason Lotvin, A. Krishna Prasad, Isis Kanevsky, Kathrin U. Jansen, Annaliesa S. Anderson, Robert G. K. Donald
Summary: Multivalent O-antigen polysaccharide glycoconjugate vaccines are important in preventing invasive infections caused by pathogenic Enterobacteriaceae. The use of high-molecular-mass O25b long-chain lattice conjugates has been shown to improve immunogenicity and protect animals from lethal challenges. Moreover, constructing long-chain lattice conjugates with two chemistries can elicit robust immune responses in monkeys.
INFECTION AND IMMUNITY
(2022)
Article
Pediatrics
Razieh Shahbazi, Siavosh Salmanzadeh-Ahrabi, Mohammad Mehdi Aslani, Masoud Alebouyeh, Jamal Falahi, Vajihe Sadat Nikbin
Summary: This study investigated the prevalence, clonality, virulence capacity, and antibiotic resistance phenotype and genotype of E. coli O25-B2-ST131 in children with urinary tract infection in Tehran, Iran. The results showed a higher frequency of antibiotic resistance and ESBLs production in O25-B2-ST131 strains compared to non-O25-B2-ST131 strains. The study confirmed the involvement of MDR-ESBLs producing E. coli strain O25-B2-ST131 in the occurrence of UTIs among children and highlighted the need for source tracking and control measures.
Article
Biochemistry & Molecular Biology
Michael Kowarik, Michael Wetter, Micha A. Haeuptle, Martin Braun, Michael Steffen, Stefan Kemmler, Neil Ravenscroft, Gianluigi De Benedetto, Matthias Zuppiger, Dominique Sirena, Paola Cescutti, Michael Wacker
Summary: This study analyzed the genetic and structural characteristics of clinical urine isolates from Switzerland, with approximately 75% of these isolates belonging to the O25B substructure. To address the high occurrence of O25B in clinical isolates, an O25B glycoconjugate vaccine was developed. The conjugate was successfully prepared and characterized, providing the potential for a multivalent E. coli conjugate vaccine containing O25B-EPA.
GLYCOCONJUGATE JOURNAL
(2021)
Article
Microbiology
Luciana Sampaio Lima, Aldo Aparecido Proietti-Junior, Yan Correa Rodrigues, Marcelo Cleyton da Silva Vieira, Luana Nepomuceno Gondim Costa Lima, Cintya de Oliveira Souza, Veronica Dias Goncalves, Marcelo de Oliveira Lima, Dalia dos Prazeres Rodrigues, Karla Valeria Batista Lima
Summary: This study investigated the prevalence of quinolone-resistant Escherichia coli in fish species in the Brazilian Amazon. The isolates exhibited multidrug resistance, virulence, and genetic diversity. The results suggest that fish species in the Brazilian Amazon may serve as a reservoir for antimicrobial-resistant strains.
Article
Microbiology
Yeliz Tanriverdi Cayci, Kubra HaciemInoglu Ulker, Gulsah Karacan Temur, Doga Beliz Guney, Mustafa Ertokatli, Asuman Birinci
Summary: This study investigated the prevalence of the O25b-ST131 clone and the presence of carbapenem and fosfomycin resistance genes in fluoroquinolone-resistant E.coli isolates. The results showed that the prevalence of E.coli O25b-ST131 isolates was 51.2%, with a carbapenem resistance rate of 3.41% and a fosfomycin resistance rate of 3.41% in fluoroquinolone-resistant E.coli isolates. This study is important for understanding resistance patterns and guiding treatment strategies.
MIKROBIYOLOJI BULTENI
(2023)
Article
Infectious Diseases
Ahlem Jouini, Amira Klibi, Imen Elarbi, Meriem Ben Chaabene, Safa Hamrouni, Oussema Souiai, Mariem Hanachi, Abdeljelil Ghram, Abderrazak Maaroufi
Summary: The study found ESBL and AmpC beta-lactamase producing multi-resistant E. coli isolates in poultry with diarrhea in Tunisia. These strains carried various beta-lactamase genes, including the high-risk ST131-CTX-M-15-O25-B2 clone.
Article
Biotechnology & Applied Microbiology
Jeannete Zurita, Gabriela Sevillano, Ariane Paz y Mino, Nathali Haro, Marco Larrea-Alvarez, Iliana Alcocer, David Ortega-Paredes
Summary: This study found an increase in ESBL-producing E. coli in bloodstream infections over the past 10 years, with a certain level of correlation between the isolates found during this period. The majority of these isolates were resistant to ceftriaxone, cefepime, ceftazidime, ampicillin/sulbactam, piperacillin/tazobactam, and ciprofloxacin, but not carbapenems. Over 80% of the isolates were classified as extraintestinal pathogenic E. coli, and the combination of virulence traits:papA-papC-kpsMII-uitA was the most common. Phylogroup B2 was the most prevalent, and the bacteria predominantly belonged to ST131.
JOURNAL OF APPLIED MICROBIOLOGY
(2023)
Article
Pharmacology & Pharmacy
Mustafa M. Abdulkareem, Masood A. Abdulrahman, Najim A. Yassin
Summary: This study aimed to detect five plasmid-mediated quinolone resistance (PMQR) genes in fluoroquinolone (FQs)-resistant Klebsiella pneumoniae isolated from clinical specimens. Among 120 K. pneumoniae isolates, 68 FQs-resistant isolates were included in the molecular study. The results showed a high prevalence of PMQR genes, with the Aac (6')-Ib-cr gene being the most predominant.
Article
Infectious Diseases
Amel Mhaya, Rahma Trabelsi, Sabine Aillerie, Fatima M'Zali, Dominique Begu, Slim Tounsi, Radhouane Gdoura, Corinne Arpin
Summary: Multiple ESBL-producing strains were identified in Tunisian patients, including two emerging virulent clones, ST131-C2 and ST131-C1-M27, which were found to be prevalent in both community and hospital settings.
Article
Immunology
Sajjad Asgharzadeh, Rezvan Golmoradi Zadeh, Majid Taati Moghadam, Hamed Farahani Eraghiye, Behrooz Sadeghi Kalani, Faramarz Masjedian Jazi, Shiva Mirkalantari
Summary: This study evaluated the distribution and expression of virulence genes and genotyping of E. coli O25b/ST131 among UTI patients in Tehran hospitals, Iran. The results showed a high prevalence of E. coli O25b/ST131 isolates, with high antibiotic resistance rate and biofilm formation ability.
ACTA MICROBIOLOGICA ET IMMUNOLOGICA HUNGARICA
(2022)
Article
Biotechnology & Applied Microbiology
M. Zelendova, M. Dolejska, M. Masarikova, I Jamborova, J. Vasek, J. Smola, I Manga, A. Cizek
LETTERS IN APPLIED MICROBIOLOGY
(2020)
Article
Veterinary Sciences
Aneta Papouskova, Martina Masarikova, Adam Valcek, David Senk, Darina Cejkova, Eva Jahodarova, Alois Cizek
BMC VETERINARY RESEARCH
(2020)
Article
Microbiology
Martina Masarikova, Ivana Simkova, Martin Plesko, Veronika Eretova, Marcela Krutova, Alois Cizek
Article
Biochemistry & Molecular Biology
Sarka Pospisilova, Ivan Malik, Jana Curillova, Hana Michnova, Lucie Cerna, Tereza Padrtova, Jan Hosek, Daniel Pecher, Alois Cizek, Josef Jampilek
BIOORGANIC CHEMISTRY
(2020)
Article
Biochemistry & Molecular Biology
Zuzana Hricoviniova, Sarka Mascaretti, Jana Hricoviniova, Alois Cizek, Josef Jampilek
Summary: The synthetic unnatural gallotannins showed excellent antioxidant and radical-scavenging properties, moderate antibacterial effects against S. aureus and E. faecalis, and were practically inactive against mycobacteria. However, they were efficient inhibitors of S. aureus biofilms at sub-MIC concentrations and interacted with quorum-sensing systems in certain bacteria.
Article
Biochemistry & Molecular Biology
Anna Empel, Andrzej Bak, Violetta Kozik, Malgorzata Latocha, Alois Cizek, Josef Jampilek, Kinga Suwinska, Aleksander Sochanik, Andrzej Zieba
Summary: In this study, a series of new tertiary phenothiazine derivatives were synthesized by reacting 1-methyl-3-benzoylthio-4-butylthioquinolinium chloride with 3-aminopyridine derivatives. The structures of the compounds were analyzed and their anticancer and antibacterial activities were tested. Computational analysis, shape-based comparison, and numerical sampling provided insights into the structure-activity relationships of these novel compounds.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Tomas Strharsky, Dominika Pindjakova, Jiri Kos, Lucia Vrablova, Hana Michnova, Jan Hosek, Nicol Strakova, Veronika Lelakova, Lenka Leva, Lenka Kavanova, Michal Oravec, Alois Cizek, Josef Jampilek
Summary: A series of compounds were designed and characterized, showing good antibacterial and anticancer activities with low cytotoxicity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Microbiology
Alois Cizek, Martina Masarikova, Jan Mares, Marie Brajerova, Marcela Krutova
Summary: A diverse spectrum of C. difficile strains was found in wastewater and surface water. Interestingly, a recently discovered plasmid-mediated resistance to metronidazole was detected in C. difficile from river water.
MICROBIOLOGY SPECTRUM
(2022)
Article
Chemistry, Medicinal
Tomas Gonec, Dominika Pindjakova, Lucia Vrablova, Tomas Strharsky, Hana Michnova, Tereza Kauerova, Peter Kollar, Michal Oravec, Izabela Jendrzejewska, Alois Cizek, Josef Jampilek
Summary: The prepared carbamate compounds showed high activity against Staphylococcus aureus and methicillin-resistant S. aureus (MRSA), with most compounds having a high selectivity index. However, these compounds were ineffective against Enterococcus faecalis.
Article
Biochemistry & Molecular Biology
Dominika Pindjakova, Eliska Pilarova, Karel Pauk, Hana Michnova, Jan Hosek, Pratibha Magar, Alois Cizek, Ales Imramovsky, Josef Jampilek
Summary: Two compounds (3e and 3f) derived from benzylated intermediates and target compounds showed broad-spectrum activity against reference and clinical isolates of bacteria and mycobacteria. They exhibited high activity against Staphylococcus aureus, vancomycin-resistant Enterococcus faecalis, Mycobacterium tuberculosis, and M. smegmatis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Tomas Strharsky, Dominika Pindjakova, Jiri Kos, Lucia Vrablova, Pavel Smak, Hana Michnova, Tomas Gonec, Jan Hosek, Michal Oravec, Izabela Jendrzejewska, Alois Cizek, Josef Jampilek
Summary: A series of 32 anilides with antimicrobial activity were prepared by microwave-assisted synthesis, showing potential for antibacterial applications against Staphylococcus aureus and Enterococcus faecalis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Ewa Kisiel-Nawrot, Dominika Pindjakova, Malgorzata Latocha, Andrzej Bak, Violetta Kozik, Kinga Suwinska, Aleksander Sochanik, Alois Cizek, Josef Jampilek, Andrzej Zieba
Summary: A new method for modifying the structure of tetracyclic quinobenzothiazinium derivatives has been developed, and a series of new compounds with antibacterial activity have been synthesized. These compounds showed comparable activity to existing antibacterials and low cytotoxicity to human cells, indicating their potential for further investigation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Agriculture, Dairy & Animal Science
Aneta Papouskova, Ivan Rychlik, Danka Harustiakova, Alois Cizek
Summary: An experimental group of one-day-old commercial hatchery chickens were given a defined mixture of 7 gut anaerobes. The next day, the chickens were inoculated with a ciprofloxacin-resistant APEC O78:H4-ST117 strain, alongside a control group, and monitored for 4 weeks for colonization with the inoculated strains and ciprofloxacin-resistant E. coli. The results showed a significant reduction in colonization rates of ciprofloxacin-resistant E. coli in the experimental group during the first 2 weeks. This suggests that colonization of chickens with defined anaerobic mixtures provides decisive protection during the critical period of intestinal microflora development.
Article
Veterinary Sciences
David Senk, Aneta Papouskova, Martina Masarikova, Jana Palkovicova, Alois Cizek
Summary: This study aimed to investigate the additional protective effect of an autogenous E. coli vaccine in broiler breeders pre-vaccinated with a licensed E. coli vaccine. The results showed that the autogenous vaccine could decrease the occurrence of avian colibacillosis in broiler breeders.
ACTA VETERINARIA BRNO
(2022)
Article
Microbiology
Stanislava Kralova, Lenka Davidova-Gerzova, Adam Valcek, Matej Bezdicek, Ivan Rychlik, Veronika Rezacova, Alois Cizek
Summary: This study analyzed three difficult-to-cultivate and strictly anaerobic strains and proposed classifying them into two new genera.
MICROBIOLOGY SPECTRUM
(2022)
