Article
Biochemistry & Molecular Biology
Nguyen-Hung Le, Patricia Constant, Samuel Tranier, Virginie Nahoum, Valerie Guillet, Laurent Maveyraud, Mamadou Daffe, Lionel Mourey, Pierre Verhaeghe, Hedia Marrakchi
Summary: Tuberculosis is a global health crisis with limited treatment options and drug resistance issues. Developing drugs targeting enzymes involved in cell envelope synthesis of Mycobacterium tuberculosis is crucial. Through a drug repurposing campaign, salicylanilide closantel and its derivatives were discovered as potential candidates for novel anti-tubercular drugs.
BIOORGANIC & MEDICINAL CHEMISTRY
(2022)
Article
Microbiology
Min Xie, Uday S. Ganapathy, Tian Lan, Paulina Osiecki, Jansy P. Sarathy, Veronique Dartois, Courtney C. Aldrich, Thomas Dick
Summary: Necrotic lesions and cavities filled with caseum are characteristic of mycobacterial pulmonary disease. Bronchocavitary Mycobacterium abscessus disease is difficult to treat. M. abscessus in caseum enters a prolonged stationary phase, becoming tolerant to most current antibiotics, suggesting that caseum persisters contribute to the ineffective treatments. Novel ADP-ribosylation-resistant rifabutin analogs exhibit bactericidal activity against these M. abscessus persisters at concentrations achievable by rifamycins in caseum.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2023)
Article
Microbiology
Mohammad Naiyaz Ahmad, Tanu Garg, Shriya Singh, Richa Shukla, Pradip Malik, Ramya V. Krishnamurthy, Parvinder Kaur, Sidharth Chopra, Arunava Dasgupta
Summary: This study demonstrates that gepotidacin has potent activity against mycobacterial pathogens, including M. tuberculosis and NTM, and can bypass existing resistance mechanisms. It exhibits concentration-dependent bactericidal activity, synergizes with other drugs, and reduces bacterial load similarly to amikacin in intracellular assays. Importantly, M. fortuitum ATCC 6841 is unable to develop resistance to gepotidacin. In a murine infection model, gepotidacin significantly reduces bacterial load in various organs at a lower concentration compared to amikacin.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Chemistry, Multidisciplinary
Anqi Yang, Xiang Lin, Zijian Liu, Xin Duan, Yurou Yuan, Jiaxuan Zhang, Qilin Liang, Xianglin Ji, Nannan Sun, Huajun Yu, Weiwei He, Lili Zhu, Bingzhe Xu, Xudong Lin
Summary: A novel in vivo screening strategy combining hierarchically structured biohybrid triboelectric nano-generators (HB-TENGs) arrays with computational bioinformatics analysis for high-throughput pharmacological evaluation using Caenorhabditis elegans is described. The animals' behaviors are efficiently converted into friction deformation through HB-TENGs, and the resulting triboelectric signals are extracted for various bioinformation of the screened compounds. The information-rich electrical readouts are sufficient to predict a drug's identity by multiple-Gaussian-kernels-based machine learning methods.
Article
Biochemistry & Molecular Biology
Ankita Kumari, Xin-An Zeng, Abdul Rahaman, Muhammad Adil Farooq, Yanyan Huang, Mahafooj Alee, Runyu Yao, Murtaza Ali, Ibrahim Khalifa, Omnia Badr
Summary: This study developed a simple and efficient in vivo assay using transgenic zebrafish larvae to identify compounds that can modulate M-cell regeneration. Through screening and in silico analysis, FDA-approved drugs with the potential to inhibit axon regeneration were identified, and PTEN and SOCS3 were identified as potential signaling molecules responsible for reducing axon regeneration. Additionally, the study demonstrated the toxic effects of these compounds.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Immunology
Dong-Mei Wang, Hong Liu, Yong-Li Zheng, Yuan-Hong Xu, Yi Liao
Summary: This study provides a summary of the epidemiological characteristics, species distribution, and drug sensitivity of clinical nontuberculous mycobacteria (NTM) isolates at the Public Health Clinical Center of Chengdu, China. The study found that the isolation rate of NTM has been increasing in recent years, particularly among elderly patients with a high HIV co-infection rate. The main NTM isolates were identified as Mycobacterium avium-intracellulare (MAC) and M. chelonae/M. abscessus. The study also identified effective antibiotics against different NTM strains.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Microbiology
Francoise Roquet-Baneres, Mattheo Alcaraz, Claire Hamela, Jan Abendroth, Thomas E. Edwards, Laurent Kremer
Summary: Researchers have discovered that a compound called NITD-916 displays potent antimicrobial activity against M. fortuitum both in vitro and in vivo. This compound inhibits the growth of M. fortuitum by targeting InhA(MFO) and shows promising effectiveness against drug resistance. This study suggests that NITD-916 could be a potential drug for the treatment of M. fortuitum pulmonary diseases.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2023)
Article
Chemistry, Multidisciplinary
Meghan J. O'Melia, Adriana Mulero-Russe, Jihoon Kim, Alyssa Pybus, Deborah DeRyckere, Levi Wood, Douglas K. Graham, Edward Botchwey, Andres J. Garcia, Susan N. Thomas
Summary: Immunotherapy is a powerful anti-cancer therapy, but current preclinical models have limitations. To overcome this, the authors used engineered biomaterials as scaffolds to alter the immune cells within tumors, resulting in improved tumor formation rate, decreased disease latency, and reduced variability of immune infiltrates. These findings highlight the significant role of the local immune microenvironment in immunotherapeutic response.
ADVANCED MATERIALS
(2022)
Article
Microbiology
Nhi Van, Yonatan N. Degefu, Pathricia A. Leus, Jonah Larkins-Ford, Jacob Klickstein, Florian P. Maurer, David Stone, Husain Poonawala, Cheleste M. Thorpe, Trever C. Smith, Bree B. Aldridge
Summary: New effective drug combinations have been found through the study of strain-specific drug interactions and the vast drug combination space, which can help improve the treatment of M. abscessus infections.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2023)
Article
Biochemical Research Methods
Danielle Maeser, Robert F. Gruener, Rong Stephanie Huang
Summary: Cell line drug screening datasets can be used for various drug discovery applications, including correcting drug sensitivity levels, predicting clinical drug response, and associating predictions with clinical features for drug biomarker discovery. These methodologies have been unified and updated into the OncoPredict R package to facilitate tool development and adoption, applicable for drug and biomarker discovery in various in vitro and in vivo contexts.
BRIEFINGS IN BIOINFORMATICS
(2021)
Article
Immunology
Md Shah Alam, Ping Guan, Yuting Zhu, Sanshan Zeng, Xiange Fang, Shuai Wang, Buhari Yusuf, Jingran Zhang, Xirong Tian, Cuiting Fang, Yamin Gao, Mst Sumaia Khatun, Zhiyong Liu, H. M. Adnan Hameed, Yaoju Tan, Jinxing Hu, Jianxiong Liu, Tianyu Zhang
Summary: This study identified MF GZ001 as a novel member of the M. fortuitum complex and found genetic mutations in drug resistance genes. It also discovered potential virulence genes in MF GZ001 that are shared with highly pathogenic mycobacterial species.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Dabing Chen, Wenjun Huang, Qinfang Zhang, Zenghui Zhang, Yiwang Guo, Gerrit Vreeman, Changquan Calvin Sun, Michael Hawley, Bing-Shiou Yang, Xiaorong He
Summary: This study focuses on improving bioavailability through cocrystal engineering, particularly on understanding the supersaturation maintenance mechanism during dissolution. The experimental results suggest that the cocrystal of BI 639667 with salicylic acid (SA) can better compete against water for interactions with the API and inhibit nucleation, enhancing bioavailability.
CRYSTAL GROWTH & DESIGN
(2022)
Article
Medicine, Research & Experimental
Riti Sharan, Shashank R. Ganatra, Dhiraj K. Singh, Journey Cole, Taylor W. Foreman, Rajesh Thippeshappa, Charles A. Peloquin, Vinay Shivanna, Olga Gonzalez, Cheryl L. Day, Neel R. Gandhi, Edward J. Dick, Shannan Hall-Ursone, Smriti Mehra, Larry S. Schlesinger, Jyothi Rengarajan, Deepak Kaushal
Summary: This study assessed the clearance of M. tuberculosis bacteria in macaques with asymptomatic LTBI following a three-month treatment of 3HP. The results showed that most of the treated macaques did not have persistent bacterial infection or extrapulmonary spread, while the untreated macaques harbored persistent bacterial infection and experienced tuberculosis reactivation following SIV coinfection. This suggests that 3HP treatment effectively reduces tuberculosis infection and prevents reactivation of latent tuberculosis in macaques.
JOURNAL OF CLINICAL INVESTIGATION
(2022)
Article
Medicine, Research & Experimental
Riti Sharan, Shashank R. Ganatra, Dhiraj K. Singh, Journey Cole, Taylor W. Foreman, Rajesh Thippeshappa, Charles A. Peloquin, Vinay Shivanna, Olga Gonzalez, Cheryl L. Day, Neel R. Gandhi, Edward J. Dick Jr, Shannan Hall-Ursone, Smriti Mehra, Larry S. Schlesinger, Jyothi Rengarajan, Deepak Kaushal
Summary: The study found that 3HP treatment significantly reduced the infection of Mycobacterium tuberculosis and prevented reactivation of tuberculosis in latently infected macaques.
JOURNAL OF CLINICAL INVESTIGATION
(2022)
Article
Oncology
Mariaserena Giliberto, Deepak B. Thimiri Govinda Raj, Andrea Cremaschi, Sigrid S. Skanland, Alexandra Gade, Geir E. Tjonnfjord, Fredrik Schjesvold, Ludvig A. Munthe, Kjetil Tasken
Summary: The management of multiple myeloma (MM) is challenging, and personalized testing tools are required to determine drug sensitivities. In this study, a drug testing pipeline was established to examine ex vivo drug responses in MM cells from 44 patients. Variability in drug responses and synergistic effects of certain drug combinations were observed.
MOLECULAR ONCOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Ayushi Sharma, Jitendraa Vashistt, Rahul Shrivastava
Summary: This study explored the impact of agitation, temperature, and pH on the formation of Mycobacterium fortuitum biofilm, with response surface modeling revealing that the maximum biofilm was generated at 37 degrees C and pH 7.0 under static conditions. Statistically significant results indicate that the optimal biofilm production occurred 96 hours after mycobacterial inoculation, providing a basis for utilizing response surface methodology for efficient optimization in biofilm assays. This approach can be valuable in screening potential anti-biofilm compounds and inhibitors for pathogenic mycobacteria.
Article
Engineering, Multidisciplinary
Saurav Dixit, Anna Stefanska, Adam Musiuk, Priyanka Singh
Summary: Effective communication and coordination are essential for the successful management of construction projects. The study focuses on analyzing the incorporation of information and communication technologies in the Indian built environment sector, highlighting potential advantages and improvements. However, the study also addresses various barriers hindering the use of ICT in construction project management.
AIN SHAMS ENGINEERING JOURNAL
(2021)
Article
Biochemistry & Molecular Biology
Priyanka Singh, Krishnananda Samanta, Ndeye Mathy Kebe, Gregory Michel, Baptiste Legrand, Vera E. Sitnikova, Andrey Kajava, Michel Pages, Patrick Bastien, Christelle Pomares, Olivier Coux, Jean-Francois Hernandez
Summary: Developing less toxic and more efficient treatments for leishmaniases and trypanosomiases is urgent. By targeting the parasite mitochondrial HslVU protease, which is essential for growth and has no analogue in the human host, we have developed compounds potentially inhibiting the complex assembly. Through the study of the structure-activity relationships, we have obtained more potent analogues. Additionally, conjugates of the analogues and a mitochondrial penetrating peptide have been found to penetrate into the parasite and inhibit its growth without toxicity to human cells.
BIOORGANIC CHEMISTRY
(2022)
Article
Microbiology
Monika Choudhary, Shubham Kaushik, Arti Kapil, Rahul Shrivastava, Jitendraa Vashistt
Summary: This study identified key proteins associated with biofilm formation in Acinetobacter baumannii, including adhesion factors, metabolism, nutrient transporters, and secretion system proteins. These findings provide insights into the mechanisms of biofilm formation in A. baumannii and offer potential targets for developing new clinical interventions against biofilm-associated infections.
ARCHIVES OF MICROBIOLOGY
(2022)
Review
Medicine, General & Internal
Anupam Dhasmana, Swati Dhasmana, Sudhir Kotnala, Anukriti, Vivek K. Kashyap, Poornima D. Shaji, Partha Laskar, Sheema Khan, Rinaldo Pellicano, Sharmila Fagoonee, Shafiul Haque, Murali M. Yallapu, Subhash C. Chauhan, Meena Jaggi
Summary: Gastrointestinal (GI) cancers are a leading cause of death globally. The immune system, although highly efficient in detecting and eliminating tumor cells, can be evaded by cancer cells. Therefore, cancer immunotherapy may play a crucial role in training the immune system to fight against cancer.
Article
Pharmacology & Pharmacy
Vivek K. Kashyap, Godwin Peasah-Darkwah, Anupam Dhasmana, Meena Jaggi, Murali M. Yallapu, Subhash C. Chauhan
Summary: This review highlights the potential of Withania somnifera (WS) as an anticancer and immunomodulatory molecule. Several preclinical studies have shown that WS can prevent or slow the progression of cancer originating from various organs. However, stability, bioavailability, and target specificity are major obstacles in the application of WS for cancer therapy.
Article
Microbiology
Ayushi Sharma, Jitendraa Vashistt, Rahul Shrivastava
Summary: The study identified a gene MFfabG4 that is involved in the formation of M. fortuitum colony and biofilm, and validated its role in the fatty acid biosynthesis pathway. Knocking down MFfabG4 resulted in decreased colony and biofilm formation, impaired sliding motility and cell sedimentation, and decreased cell surface hydrophobicity. The study highlights the importance of MFfabG4 as a drug target and a potential diagnostic marker.
JOURNAL OF BASIC MICROBIOLOGY
(2022)
Review
Genetics & Heredity
Deepak Parashar, Anupam Singh, Saurabh Gupta, Aishwarya Sharma, Manish K. Sharma, Kuldeep K. Roy, Subhash C. Chauhan, Vivek K. Kashyap
Summary: Cervical cancer is a common cancer in women worldwide. It can be prevented through vaccination, screening, and treatment. However, prognosis for advanced or metastatic cervical cancer is poor and current treatment options are limited. Understanding the mechanisms of metastasis and finding new therapeutic targets are crucial. Non-coding RNAs play important roles in transcriptional processes and are considered key regulators of biological processes. Recent studies have revealed the significant roles of ncRNAs in the progression, metastasis, treatment, and diagnosis of cervical cancer.
Article
Biochemistry & Molecular Biology
Monika Choudhary, Rahul Shrivastava, Jitendraa Vashistt
Summary: The study identified eugenol and geraniol as compounds with antimicrobial and antibiofilm properties against A. baumannii. They showed significant inhibition of growth and biofilm formation, indicating their potential as potent antibiofilm agents.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Microbiology
Monika Choudhary, Rahul Shrivastava, Jitendraa Vashistt
Summary: This study investigated the impact of biofilm formation by multidrug-resistant Acinetobacter baumannii on antimicrobial resistance and survival under desiccation. The results showed that most isolates formed high biofilm and eradicating mature biofilm required significantly higher concentrations of antibiotics. These findings are important for developing preventive measures against biofilm-associated A. baumannii infections.
CURRENT MICROBIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Vishal Khandelwal, Tarubala Sharma, Saurabh Gupta, Shoorvir Singh, Manish Kumar Sharma, Deepak Parashar, Vivek K. Kashyap
Summary: The emergence and re-emergence of infectious viral agents in the past decades, including flaviviruses, influenza, filoviruses, alphaviruses, and coronaviruses, have posed serious threats to public health. Although various medicinal compounds are being investigated for the treatment of these viral diseases, lung damage caused by SARS-CoV-2 remains a major concern. Stem cell therapy, with its antioxidant and immunomodulatory properties, is considered a novel approach to regenerate damaged lungs and reduce inflammation. Clinical studies have shown positive implications for the use of stem cell therapy in treating virally induced infections in patients.
MOLECULAR BIOLOGY REPORTS
(2022)
Article
Biology
Mehdi Chaib, Bilal B. Hafeez, Hassan Mandil, Deidre Daria, Ajeeth K. Pingili, Sonam Kumari, Mohammed Sikander, Vivek K. Kashyap, Guo-Yun Chen, Emmanuel Anning, Manish K. Tripathi, Sheema Khan, Stephen Behrman, Murali M. Yallapu, Meena Jaggi, Liza Makowski, Subhash C. Chauhan
Summary: This study discovers that a probiotic-derived siderophore, ferrichrome, can remodel tumor-associated macrophages, increase CD8+ T cell infiltration, and significantly reduce tumor burden in pancreatic cancer. Ferrichrome also enhances the efficacy of anti-PD-L1 therapy, providing a potential novel combination therapy for pancreatic cancer treatment.
COMMUNICATIONS BIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Ayushi Sharma, Saurabh Bansal, Neha Kumari, Jitendraa Vashistt, Rahul Shrivastava
Summary: This study conducted a comprehensive proteome analysis of M. fortuitum under planktonic and biofilm growth states, revealing previously unexplored biochemical pathways associated with lipid biosynthesis and quorum-sensing. The interaction network of proteins during biofilm growth state and functional alliance of hypothetical proteins were investigated. The study also identified biomarkers associated with the planktonic and biofilm growth of M. fortuitum.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Infectious Diseases
Ayushi Sharma, Jitendraa Vashistt, Rahul Shrivastava
Summary: The MFhadC gene in Mycobacterium fortuitum is found to interact with proteins involved in biofilm formation, but it may not be a major virulence factor. MFhadC is important for the survival of M. fortuitum under nutrient-starved conditions, but it is not a biofilm-specific marker for this bacteria.
INTERNATIONAL JOURNAL OF MYCOBACTERIOLOGY
(2022)
Article
Engineering, Environmental
Pavan Kumar Agrawal, Pooja Upadhyay, Rahul Shrivastava, Swati Sharma, Vijay Kumar Garlapati
Summary: This study demonstrated the efficiency of an endophytic fungus Penicillium megasporum NFCCI3977 in decolorization of synthetic textile dyes, showing promising results for Congo red, orange G, and rhodamine B. The research findings highlight the potential of biological approaches for proper disposal of hazardous synthetic textile dyes into the environment.
JOURNAL OF HAZARDOUS TOXIC AND RADIOACTIVE WASTE
(2021)