Review
Infectious Diseases
Mihret Tilahun, Yeshimebet Kassa, Alemu Gedefie, Melaku Ashagire
Summary: Infections due to multidrug-resistant Enterobacteriaceae have become a major global public health issue, with transmission facilitated by inappropriate antimicrobial use and increased global connectivity. Carbapenems, the current medications of choice, are facing increasing resistance globally, necessitating the search for alternative treatment options. Ceftazidime-avibactam is currently the most common treatment option, with other novel drugs under trial for different populations. Treatment should be tailored to patient susceptibility, infection type, and personal characteristics.
INFECTION AND DRUG RESISTANCE
(2021)
Article
Microbiology
Elizabeth Story-Roller, Christos Galanis, Gyanu Lamichhane
Summary: Mycobacteroides abscessus is a difficult-to-treat environmental pathogen that can cause chronic pulmonary disease in patients with underlying structural lung conditions. Current treatment options are often ineffective and associated with significant toxicities, with the pathogen naturally resistant to many antibiotics.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Infectious Diseases
Roman Kozlov, Alexey Kuzmenkov
Summary: The study in Russia found an increasing trend in antimicrobial resistance among Enterobacteriaceae, with the lowest susceptibility to colistin, ampicillin, and ampicillin/sulbactam, and the best susceptibility to combinations containing avibactam.
Article
Agriculture, Dairy & Animal Science
Nikolaos Tsekouras, Zoi Athanasakopoulou, Celia Diezel, Polychronis Kostoulas, Sascha D. Braun, Marina Sofia, Stefan Monecke, Ralf Ehricht, Dimitris C. Chatzopoulos, Dominik Gary, Domenique Kraehmer, Vassiliki Spyrou, Georgios Christodoulopoulos, Charalambos Billinis, Vasileios G. Papatsiros
Summary: This study aimed to determine the prevalence of ESBL-producing bacteria in swine in Greece. Fecal samples were collected from 214 piglets on 34 farms, and 78 isolates (36.5%) were identified as ESBL producers, including E. coli, K. pneumoniae, P. mirabilis, E. cloacae complex, and S. enterica spp. diarizonae. Resistance to non-beta-lactam antibiotics was detected in all isolates, with various resistant phenotypes observed. Molecular analysis revealed the presence of multiple resistance genes. The high levels of antimicrobial resistance observed in Greek swine herds raise concerns for both animal and public health.
Article
Infectious Diseases
Stephanie Gross, Anja Mueller, Diana Seinige, Peter Wohlsein, Manuela Oliveira, Dieter Steinhagen, Corinna Kehrenberg, Ursula Siebert
Summary: Antimicrobial resistance is a global health threat with wildlife acting as a reservoir and vector for resistant bacteria and genes. This study found resistant strains of Escherichia coli in marine mammals of the North and Baltic Seas, highlighting their role as sentinels for antimicrobial-resistant bacteria. The distribution of resistant bacteria in these sea areas provides important information from a One Health perspective.
Article
Infectious Diseases
Atnafu Bushen, Eyob Tekalign, Mengistu Abayneh
Summary: This study evaluated the drug- and multidrug resistance patterns of Enterobacteriaceae isolated from chicken droppings at a university farm in southwest Ethiopia. The findings revealed common presence of E. coli, K. pneumoniae, P. mirabilis, and Salmonella species, with high resistance rates observed against certain antibiotics, highlighting the potential role of chickens in transmitting antibiotic-resistant bacteria to humans through the food chain.
INFECTION AND DRUG RESISTANCE
(2021)
Article
Infectious Diseases
Carolina Pantuzza Ramos, Carolina Yumi Iceri Kamei, Flavia Mello Viegas, Jonata de Melo Barbieri, Joao Luis Reis Cunha, Yaovi Mahuton Gildas Hounmanou, Fernanda Morcatti Coura, Jordana Almeida Santana, Francisco Carlos Faria Lobato, Anders Miki Bojesen, Rodrigo Otavio Silveira Silva
Summary: Feeding dogs raw meat-based diets (RMBDs) can affect the microbiota, increase shedding of diarrheagenic E. coli, and contribute to the spread of multidrug-resistant strains harboring mobilizable colistin resistance and ESBL genes, posing a concern for both animal and human health.
Review
Biochemistry & Molecular Biology
Oluwaseun Ola Adeniji, Nolonwabo Nontongana, Janet Chiyem Okoh, Anthony Ifeanyi Okoh
Summary: Antibiotic resistance is a global public health concern, and finding new treatment methods is important. Combining antibiotics and using nanoparticles may have potential in treating multidrug-resistant bacterial infections.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Immunology
Xinhua Luo, Zhe Yin, Lijun Zeng, Lingfei Hu, Xiaoyuan Jiang, Ying Jing, Fangzhou Chen, Dongguo Wang, Yajun Song, Huiying Yang, Dongsheng Zhou
Summary: In this study, a detailed genetic dissection of the bla (NDM)-carrying genetic elements and their related mobile genetic elements in Enterobacteriaceae revealed the presence of numerous resistance genes and complex structures. The analysis identified eight novel mobile elements, including ICEs, IMEs, composite transposons, and an integron.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Infectious Diseases
Anna Olsson, Lisa Allander, Ayda Shams, Hissa Al-Farsi, Pernilla Lagerback, Thomas Tangden
Summary: This study assessed the effects of polymyxin B combinations against carbapenemase-producing Klebsiella pneumoniae (K. pneumoniae). Synergistic and bactericidal effects were observed with polymyxin B in combination with other antibiotics. Specific genotype-phenotype associations were found. This study provides important information for the search for promising combinations and individualized treatment.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2023)
Article
Genetics & Heredity
Sahaya Glingston Rajakani, Basil Britto Xavier, Adwoa Sey, El Bounja Mariem, Christine Lammens, Herman Goossens, Youri Glupczynski, Surbhi Malhotra-Kumar
Summary: This study utilizes population analysis profiling (PAP) and time-kill assay for the detection of heteroresistance in K. pneumoniae and for evaluating the association between in vitro regrowth and heteroresistance. The mechanisms of colistin resistance and the ability of combination therapies to suppress resistance selection were also analysed.
Article
Microbiology
Luis Eduardo de Souza Gazal, Leonardo Pinto Medeiros, Miriam Dibo, Erick Kenji Nishio, Vanessa Lumi Koga, Bruna Carolina Goncalves, Tiela Trapp Grassotti, Taiara Carolaine Leal de Camargo, Joao Juliano Pinheiro, Eliana Carolina Vespero, Kelly Cristina Tagliari de Brito, Benito Guimaraes de Brito, Gerson Nakazato, Renata Katsuko Takayama Kobayashi
Summary: This study investigated the presence of ESBL-producing and fosfomycin-resistant Escherichia coli in poultry farms in southern Brazil. The findings indicate that poultry litter and beetles are critical points during poultry production and the presence of fosfomycin-resistant strains suggest risks associated with antimicrobial use. Additionally, genetic determinants encoding CTX-M and fosA3 enzymes can be transmitted to E. coli strains from broiler chicken microbiota, posing a risk to public health.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Multidisciplinary Sciences
Tewachew Awoke, Brhanu Teka, Abraham Aseffa, Shemse Sebre, Aminu Seman, Biruk Yeshitela, Tamrat Abebe, Adane Mihret
Summary: This study investigated the prevalence of carbapenemase-producing K. pneumoniae in Ethiopia and found the emergence of bla(NDM) and bla(KPC) gene-carrying strains for the first time. The prevalence was worrisome and associated with previous use of carbapenem drugs.
Article
Multidisciplinary Sciences
Marisa Zenaide Ribeiro Gomes, Elisangela Martins J. de Lima, Caio Augusto Martins Aires, Polyana Silva Pereira, Juwon Yim, Fernando Henrique Silva, Caio Augusto Santos Rodrigues, Thamirys Rachel Tavares e Oliveira, Priscila Pinho da Silva, Cristiane Monteiro Eller, Claudio Marcos Rocha de Souza, Michael Rybak, Rodolpho Mattos Albano, Antonio Basilio de Miranda, Edson Machado, Marcos Catanho
Summary: We report a PCR-Kp outbreak causing untreatable infections descriptively correlated with bacterial genomes. A target phenotypic profile was detected in 10% of the tested patients, who had epidemiological links and were involved in intractable infections and death.
SCIENTIFIC REPORTS
(2023)
Article
Infectious Diseases
Madalina Maria Muntean, Andrei-Alexandru Muntean, Francois Guerin, Vincent Cattoir, Elodie Creton, Garance Cotellon, Saoussen Oueslati, Mircea Ioan Popa, Delphine Girlich, Bogdan I. Iorga, Remy A. Bonnin, Thierry Naas
Summary: The rCIM-A can effectively differentiate between AHEs and CPEs, with a sensitivity of 84.26% and specificity of 99.29%. While it has limitations in detecting certain resistance mechanisms, it can serve as a simple, fast, and accurate method for CPE detection.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2021)
Article
Infectious Diseases
Irene Galani, Konstantina Nafplioti, Panagiota Adamou, Ilias Karaiskos, Helen Giamarellou, Maria Souli, Sofia Maraki, Viktoria Eirini Mauromanolaki, Vassiliki Papaioannou, Sofia Tsiplakou, Polyzo Kazila, Ioanna Diamanti, Nikoletta Charalampaki, Eleftheria Trikka-Graphakos, Marina Toutouza, Helen Vagiakou, Konstantinos Pappas, Anna Kyratsa, Angeliki Paschali, Konstantina Kontopoulou, Olga Legga, Efthymia Petinaki, Helen Papadogeorgaki, Efrosini Chinou
BMC INFECTIOUS DISEASES
(2019)
Correction
Infectious Diseases
Irene Galani, Konstantina Nafplioti, Panagiota Adamou, Ilias Karaiskos, Helen Giamarellou, Maria Souli
BMC INFECTIOUS DISEASES
(2019)
Article
Infectious Diseases
Konstantina Nafplioti, Irene Galani, Evdokia Angelidis, Panagiota Adamou, Eleni Moraitou, Panagiota Giannopoulou, Paraskevi Chra, Maria Damala, Evangellos Vogiatzakis, Eleftheria Trikka-Graphakos, Vasiliki Baka, Eleni Prifti, Anastasia Antoniadou, Maria Souli
MICROBIAL DRUG RESISTANCE
(2020)
Article
Infectious Diseases
Vassiliki Papoutsaki, Irene Galani, Eleni Papadimitriou, Irene Karantani, Ilias Karaiskos, Helen Giamarellou
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2020)
Article
Infectious Diseases
Irene Galani, Ilias Karaiskos, Maria Souli, Vassiliki Papoutsaki, Lamprini Galani, Aikaterini Gkoufa, Anastasia Antoniadou, Helen Giamarellou
Article
Microbiology
Su Mon Aye, Irene Galani, Heidi Yu, Jiping Wang, Ke Chen, Hasini Wickremasinghe, Ilias Karaiskos, Phillip J. Bergen, Jinxin Zhao, Tony Velkov, Helen Giamarellou, Yu-Wei Lin, Brian T. Tsuji, Jian Li
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2020)
Article
Infectious Diseases
Irene Galani, Vassiliki Papoutsaki, Irene Karantani, Ilias Karaiskos, Lamprini Galani, Panagiota Adamou, Ioannis Deliolanis, Antigoni Kodonaki, Eleni Papadogeorgaki, Margarita Markopoulou, Sofia Maraki, Maria Damala, Eleni Prifti, Eleni Vagiakou, Efthimia Petinaki, Kimon Fountoulis, Sophia Tsiplakou, Helen Kirikou, Maria Souli, Anastasia Antoniadou, Helen Giamarellou
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2020)
Article
Infectious Diseases
Irene Galani, Ilias Karaiskos, Evdokia Angelidis, Vassiliki Papoutsaki, Lamprini Galani, Maria Souli, Anastasia Antoniadou, Helen Giamarellou
Summary: Three ceftazidime-avibactam-resistant KPC-2-producing Klebsiella pneumoniae strains of ST39 were isolated in Greece, with two strains corresponding to KPC-33 and KPC-57, and the third strain corresponding to KPC-44.
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
(2021)
Article
Infectious Diseases
Konstantina Nafplioti, Maria Souli, Panagiota Adamou, Eleni Moraitou, Panagiota Giannopoulou, Paraskevi Chra, Maria Damala, Evangelos Vogiatzakis, Eleftheria Trikka-Graphakos, Vasiliki Baka, Eleni Prifti, Anastasia Antoniadou, Irene Galani
Summary: This study aimed to characterize the 16S rRNA methylase (RMT) genes in aminoglycoside-resistant Enterobacterales and Pseudomonas aeruginosa isolates in hospitals in Athens, Greece in 2015-2016. It found highly resistant isolates with confirmed ESBL and carbapenemase production, with predominant rmtB1 gene in Enterobacterales and similar IncC plasmids responsible for gene dissemination, posing a public health threat.
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
(2021)
Article
Infectious Diseases
Su Mon Aye, Irene Galani, Mei-Ling Han, Ilias Karaiskos, Darren J. Creek, Yan Zhu, Yu-Wei Lin, Tony Velkov, Helen Giamarellou, Jian Li
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2020)
Article
Infectious Diseases
I Karaiskos, G. L. Daikos, A. Gkoufa, G. Adamis, A. Stefos, S. Symbardi, G. Chrysos, E. Filiou, D. Basoulis, E. Mouloudi, L. Galani, K. Akinosoglou, K. Arvaniti, A. Masgala, M. Petraki, E. Papadimitriou, I Galani, G. Poulakou, C. Routsi, H. Giamarellou
Summary: The study demonstrates that ceftazidime/avibactam is effective in treating infections caused by KPC-Kp, significantly reducing mortality rates. Additionally, ultimately fatal disease, rapidly fatal disease, and Charlson comorbidity index ≥ 2 were identified as independent predictors of death, while treatment with ceftazidime/avibactam-containing regimens was the only independent predictor of survival.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2021)
Review
Infectious Diseases
Irene Galani, Ilias Karaiskos, Helen Giamarellou
Summary: Multi-drug-resistant Klebsiella pneumoniae is a major concern in bacterial resistance, with reports of strains developing resistance to Ceftazidime-Avibactam during treatment. Understanding the mechanisms of resistance can help improve the efficacy of current antimicrobials and guide the design of novel therapeutic agents that can overcome resistance mechanisms.
EXPERT REVIEW OF ANTI-INFECTIVE THERAPY
(2021)
Review
Infectious Diseases
Ilias Karaiskos, Irene Galani, Vassiliki Papoutsaki, Lamprini Galani, Helen Giamarellou
Summary: The emergence of carbapenemase resistant Gram-negative bacteria is a major public health concern. The review highlights new treatments for infections caused by carbapenemase producing Klebsiella pneumoniae, with a focus on novel antibiotics and beta-lactamase inhibitors. Approvals and developments of new antimicrobial agents show promising outcomes in combating CPKP infections, with potential for improved efficacy and safety profiles in future therapeutic strategies against multidrug-resistant K. pneumoniae.
EXPERT REVIEW OF ANTI-INFECTIVE THERAPY
(2022)
Article
Infectious Diseases
Irene Galani, Vassiliki Papoutsaki, Ilias Karaiskos, Nikolaos Moustakas, Lamprini Galani, Sofia Maraki, Viktoria Eirini Mavromanolaki, Olga Legga, Kimon Fountoulis, Evangelia D. Platsouka, Panagiota Giannopoulou, Helen Papadogeorgaki, Maria Damala, Efrosini Chinou, Aggeliki Pasxali, Ioannis Deliolanis, Helen Vagiakou, Efthymia Petinaki, Anastasia Chli, Eleni Vagdatli, Polyzo Kazila, Vassiliki Papaioannou, Konstantina Kontopoulou, Atalia Noemi Ferke, Eleni Moraitou, Anastasia Antoniadou, Helen Giamarellou
Summary: Resistance of A. baumannii to multiple antimicrobials in Greece is very high, making most of them obsolete. This study aimed to determine the molecular epidemiology and susceptibilities of A. baumannii isolates from different Greek hospitals. The majority of isolates produced carbapenemase OXA-23 and were assigned to sequence group G1. Apramycin and cefiderocol showed good activity against the isolates, while minocycline, colistin, and ampicillin-sulbactam had limited activity.
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
(2023)
Article
Rheumatology
Konstantinos Thomas, Ioannis Grigoropoulos, Panagiota Alexopoulou, Emmanouil Karofylakis, Irene Galani, Kyriaki Korina Papadopoulou, Anastasia Tsiavou, Aliki Ntourou, Eleftheria Mavrou, Irina Qevani, Pelagia Katsimbri, Christos Koutsianas, Evgenia Mavrea, Dimitrios Vassilopoulos, Spyros Pournaras, Sotirios Tsiodras, Dimitrios Boumpas, Anastasia Antoniadou
Summary: This study examined the immune response to SARS-CoV-2 vaccination in rheumatic patients treated with rituximab. It was found that despite undetectable anti-spike antibodies in the serum, four out of ten patients showed lasting cell-mediated immune response. The cumulative dose of rituximab had an impact on both humoral and cell-mediated responses to the vaccine. Cell-mediated immune responses may serve as an important marker of vaccine efficacy against SARS-CoV-2.