Article
Infectious Diseases
Martin Rihacek, Michaela Kuthanova, Zbynek Splichal, Vojtech Adam, Kristyna Hrazdilova, Radek Vesely, Ludek Zurek, Kristyna Cihalova
Summary: Resistance to beta-lactams, particularly ampicillin, in pathogenic Escherichia coli is increasing due to intrinsic and acquired mechanisms, including mutations and over-expression of efflux pumps in the RND superfamily. This study analyzed isolates from patients' wounds and a control strain, finding that most isolates showed phenotypic resistance to ampicillin and piperacillin, with acquired bla genes present. However, the RND-family efflux pumps did not play a major role in the phenotypic resistance to ampicillin after exposure.
INFECTION AND DRUG RESISTANCE
(2023)
Article
Microbiology
Georgios V. Zacharopoulos, Georgios A. Manios, Marios Papadakis, Dimitra Koumaki, Sofia Maraki, Dimitrios Kassotakis, Eelco De Bree, Andreas Manios
Summary: This study evaluated the antibiotic resistance of E. faecalis in surgical patients and found an increasing incidence of E. faecalis infections in the clinic. The results showed that ampicillin and teicoplanin had similar efficacy in treating E. faecalis infections, suggesting that ampicillin could be used as effective empiric therapy.
Article
Microbiology
Jose Arellano-Galindo, Sergio Zavala-Vega, Rosario Vazquez-Larios, Sara A. Ochoa, Ariadnna Cruz-Cordova, Adolfo Sierra-Santoyo, Lourdes Lopez-Gonzalez, Rigoberto Hernandez-Castro, Silvia Giono-Cerezo, Juan Xicohtencatl-Cortes
Summary: This study analyzed the effect of ampicillin on Enterococcus faecalis and found that it can modulate the expression of aggregation substances (AS). The study also observed a possible interaction between ampicillin and pheromone expression, as well as between pheromone expression and AS expression.
Article
Multidisciplinary Sciences
Zhuojun Dai, Xiaoyu Yang, Feilun Wu, Lihua Wang, Kun Xiang, Pengcheng Li, Qingqing Lv, Jinhui Tang, Anders Dohlman, Lei Dai, Xiling Shen, Lingchong You
Summary: Cell-mediated living fabrication has the potential to create materials with versatile, programmable functions. In this study, living materials consisting of semi-interpenetrating polymer networks were engineered using bacteria encapsulated in polymeric microcapsules. These materials enhance mechanical properties and anchor effector proteins for diverse applications.
NATURE COMMUNICATIONS
(2021)
Article
Microbiology
Satoshi Katada, Akira Fukuda, Chie Nakajima, Yasuhiko Suzuki, Takashi Azuma, Ayaka Takei, Hideshige Takada, Eiryu Okamoto, Toshihide Kato, Yutaka Tamura, Masaru Usui
Summary: Both aerobic composting (AC) and anaerobic digestion (AD) can significantly reduce the concentrations of antimicrobial-resistant bacteria (ARB) and antimicrobial resistance genes (ARGs), but AD is more effective than AC. Although the majority of residual antimicrobials decrease after treatment, oxytetracycline levels remain unchanged in most cases.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Spectroscopy
Joshua C. Gukowsky, Tianxi Yang, Lili He
Summary: This study successfully differentiated between ampicillin-sensitive and resistant E. coli strains using three different SERS analytical approaches, with extracellular matrix analysis and filter mapping proving to be more effective than the conventional method. There were some significant differences in the spectra obtained from the different techniques, and the potential advantages and disadvantages of each approach should be considered for future use.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Article
Infectious Diseases
Jatapat Hemapanpairoa, Dhitiwat Changpradub, Wichai Santimaleeworagun
Summary: This retrospective study evaluated the treatment outcomes and factors associated with mortality in patients with ampicillin-susceptible enterococci bloodstream infections. The study found that the 30-day mortality rate was higher in the vancomycin treatment group, and independent factors associated with 30-day mortality included the Pitt bacteremia score, age-adjusted Charlson Comorbidity Index, and vancomycin treatment.
Article
Microbiology
Lukas Hleba, Miroslava Hlebova, Eva Kovacikova, Anton Kovacik
Summary: The rapid determination of beta-lactamase presence in H. influenzae isolates was achieved through indirect detection of degradation ampicillin products using MALDI-TOF MS. This method can distinguish resistant and susceptible strains and accelerate the identification of beta-lactamase-producing strains in clinical microbiology.
Article
Chemistry, Multidisciplinary
Marco Oliveira, Silvia Soares, Sara Sa, Alvaro Gestoso, Miguel Correa-Duarte, Pilar Baylina, Ruben Fernandes, Carla F. Pereira
Summary: In this study, biosynthetic gold nanoparticles were successfully produced using Pseudomonas aeruginosa. The synthesis was confirmed through various methods, and the nanoparticles were evaluated for their antibacterial properties when combined with low concentrations of ampicillin.
APPLIED SCIENCES-BASEL
(2023)
Article
Engineering, Environmental
Wei Wang, Peter J. Vikesland
Summary: In this study, surface-enhanced Raman spectroscopy (SERS) was used to monitor the bioactive metabolites produced by ampicillin-resistant Pseudomonas aeruginosa strains and investigate the mechanisms underlying antibiotic resistance. The results showed that the blue-green pigment pyocyanin (PYO) dominated the metabolite signals and was significantly enhanced in the presence of subminimal inhibitory concentrations of ampicillin. The SERS spectra also revealed minimal impact on the production of intermediate substances and consistent nutrient consumption. Furthermore, exogenously added PYO enhanced the tolerance of ampicillin-susceptible Escherichia coli to some extent, indicating that PYO mediates antibiotic resistance in both the parent species and cocultured bacterial strains. The metabolic SERS signal provides new insights into antibiotic resistance and has promising applications in environmental monitoring and rapid clinical detection.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Article
Engineering, Environmental
Wei Wang, Peter J. Vikesland
Summary: This study demonstrates the use of surface-enhanced Raman spectroscopy (SERS) to monitor the metabolites produced by ampicillin-resistant Pseudomonas aeruginosa strains and identify the mechanisms underlying antibiotic resistance. The results indicate that the blue-green pigment pyocyanin (PYO) dominates the metabolite signals and is significantly enhanced by exposure to subminimal inhibitory concentrations of ampicillin. These findings provide new insights into antibiotic resistance.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Article
Environmental Sciences
Yue Li, Lexin Xia, Jian Chen, Yulu Lian, Ajai A. Dandekar, Feng Xu, Meizhen Wang
Summary: The study reveals a significant increase in the minimum inhibitory concentration of evolved Pseudomonas aeruginosa PAO1 under sub-lethal conditions, associated with mutations in mpl and ampD, and overexpression of quorum sensing genes. The involvement of quorum sensing in antibiotic resistance evolution is highlighted, showing a multifactorial contribution to observed phenotypes.
ENVIRONMENT INTERNATIONAL
(2021)
Article
Microbiology
Ana R. Freitas, Ana P. Tedim, Ana C. Almeida-Santos, Barbara Duarte, Houyem Elghaieb, Mohamed S. Abbassi, Abdennaceur Hassen, Carla Novais, Luisa Peixe
Summary: Through genomic analysis, researchers identified multidrug-resistant Enterococcus faecium strains causing human infections in Tunisian healthcare institutions. These strains were compared with non-human sources within the same country, revealing their spread across different hosts and regions with similar antibiotic resistance and virulence genetic patterns.
Article
Infectious Diseases
Binan Geng, Xingyu Huang, Yalun Wu, Qiaoning He, Shihui Yang
Summary: This study investigated antibiotic resistance genes in Z. mobilis using bioinformatic approaches and CRISPR/Cas12a-based genome-editing technology. Six ampicillin-resistant genes were identified, and mutant strains were constructed. The results showed that ZMO0103 played a key role in ampicillin resistance, and other ampicillin-resistant genes may have a synergetic effect with it. This study laid a foundation for further research on antibiotic resistance mechanisms.
Article
Infectious Diseases
Yanik Sierra, Aida Gonzalez-Diaz, Anna Carrera-Salinas, Damaris Berbel, Daniel Antonio Vazquez-Sanchez, Fe Tubau, Meritxell Cubero, Junkal Garmendia, Jordi Camara, Josefina Ayats, Carmen Ardanuy, Sara Marti
Summary: Through the study of urogenital and respiratory isolates of Haemophilus parainfluenzae, it was found that multidrug resistance is primarily associated with specific genes, and the presence of polysaccharide capsules in some urogenital isolates is a cause for concern.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2021)
Article
Biotechnology & Applied Microbiology
Jeremy L. Pinyon, Matthias Klugmann, Nigel H. Lovell, Gary D. Housley
HUMAN GENE THERAPY
(2019)
Article
Health Care Sciences & Services
Tony Floyd, Siri Martensson, Jannine Bailey, Derek Kay, Bruce McGarity, Bronwyn K. Brew
BMC HEALTH SERVICES RESEARCH
(2019)
Article
Biochemistry & Molecular Biology
David M. Housley, Jeremy L. Pinyon, Georg von Jonquieres, Chamini J. Perera, Michael Smout, Michael J. Liddell, Ernest A. Jennings, David Wilson, Gary D. Housley
Article
Education & Educational Research
Jannine Bailey, Sabrina Pit
BMC MEDICAL EDUCATION
(2020)
Editorial Material
Public, Environmental & Occupational Health
Philip Roberts, Catherine Cosgrave, Judy Gillespie, Christina Malatzky, Sarah Hyde, Wendy C. Y. Hu, Jannine Bailey, Tagrid Yassine, Natalie Downes
Summary: Professional preparation is influenced by specific contexts, and place has a significant impact on professionals and their practices. The discrepancy between different locations can lead to new professionals feeling inadequate, affecting the development of their careers.
AUSTRALIAN JOURNAL OF RURAL HEALTH
(2021)
Article
Public, Environmental & Occupational Health
Krista Reed, Buck Reed, Jannine Bailey, Karen Beattie, Elizabeth Lynch, Jane Thompson, Robyn Vines, Kam Cheong Wong, Tim McCrossin, Ross Wilson
Summary: Western Sydney University has implemented a rural interprofessional learning programme to promote collaborative care approaches and enhance cross-discipline communications. The programme includes simulations of complex health events and has shown to improve students' understanding of interdisciplinary care and the importance of collaborative care.
AUSTRALIAN JOURNAL OF RURAL HEALTH
(2021)
Article
Public, Environmental & Occupational Health
Charmaine D'Souza, Nikitha Kramadhari, Elizabeth Skalkos, Tegan Dutton, Jannine Bailey
Summary: Skin cancer rates in Australia, particularly in Western NSW, are high. Farmers in this region are at greater risk due to high sun exposure. While knowledge of sun safety is high among farmers, there is underestimation of the risk of developing skin cancer. Addressing perceived barriers to sun safety practices could improve compliance in this group.
Article
Public, Environmental & Occupational Health
Jannine Bailey, Ilse Blignault, Pamela Renata, Phil Naden, Sally Nathan, Jamie Newman
Summary: Developing a strong Aboriginal and Torres Strait Islander health workforce is crucial for narrowing the health outcomes gap between Indigenous and non-Indigenous Australians. Barriers faced by Aboriginal individuals in pursuing health careers include pre-employment, recruitment, workplace, and further education and training. Providing support and opportunities at every stage, as well as culturally appropriate human resource systems, are essential for enhancing career pathways for Aboriginal people.
AUSTRALIAN JOURNAL OF RURAL HEALTH
(2021)
Article
Education & Educational Research
Sabrina W. Pit, Sue Velovski, Krista Cockrell, Jannine Bailey
Summary: This study found that medical students have increased exposure and interest in telehealth since COVID-19, and wish to see telehealth training incorporated into the curriculum from the beginning of medical school. They believe telehealth should be part of medical education, and emphasize the significant role rural clinicians play in telehealth implementation.
BMC MEDICAL EDUCATION
(2021)
Article
Public, Environmental & Occupational Health
Sean Denis Hanly, Harsan Kanagaretnam, Shobhana Katti, Gajanth Sivapalan, Timothy Lung Hon Wong, Jannine Bailey
AUSTRALIAN JOURNAL OF RURAL HEALTH
(2022)
Article
Public, Environmental & Occupational Health
Christine Stevens, Tegan Dutton, Jannine Bailey
AUSTRALIAN JOURNAL OF RURAL HEALTH
(2021)
Article
Emergency Medicine
Jack Mowbray, Lima Peterson, Jannine Bailey
Summary: This study examined respiratory-related ED admissions and presentations at Bathurst Base Hospital during the 2019-2020 New South Wales bushfire crisis. The results showed that poorer air quality was correlated with increased ED presentations, and ED patients with respiratory diagnoses were more likely to be admitted for inpatient care.
EMERGENCY MEDICINE AUSTRALASIA
(2022)
Article
Public, Environmental & Occupational Health
Tiffany Tan, Sonia Chan, Melissa Ind, Georgia Pace, Jannine Bailey, Krista Reed, Teagan Dutton, Uchechukwu Levi Osuagwu, Kam Cheong Wong
Summary: This qualitative study explored the benefits and challenges of e-prescribing for GPs and pharmacists in regional NSW. The study found that e-prescribing can improve the prescribing and dispensing process, patient adherence, and prescription safety. However, challenges include concerns about safety and security, costs of software updates, and the need for education among healthcare professionals and patients.
AUSTRALIAN JOURNAL OF RURAL HEALTH
(2023)
Article
Health Care Sciences & Services
Elizabeth Gosbell, Alisha Panambalana, Annabelle Stephenson, Carissa Vici, Tegan Dutton, Jannine Bailey
Summary: Limited research has been conducted on the awareness and attitudes towards the revised National Cervical Screening Program (NCSP) among rural Australian women. This study aimed to fill this research gap by examining the awareness and attitudes of rural women in New South Wales (NSW) towards the revised NCSP. The findings highlight the importance of awareness and knowledge in shaping women's attitudes towards the program, and suggest that strategies to increase participation rates should focus on these areas.
AUSTRALIAN JOURNAL OF PRIMARY HEALTH
(2023)
Review
Physiology
Gary D. Housley, Georg von Jonquieres, Jeremy L. Pinyon, Julie-Anne T. Matheson, Lily J. Pearson, Theresa P. Salthouse, Jennie M. E. Cederholm
CURRENT OPINION IN PHYSIOLOGY
(2020)