Article
Infectious Diseases
Ryanbi Pratama, Alicia G. Beukers, Christopher J. McIver, Caitlin L. Keighley, Peter C. Taylor, Sebastiaan J. van Hal
Summary: The study revealed that the 2011 expansion of ST80 vanA VREfm isolates originated from the 2009 index isolate, transferred from overseas. Phylogenetic analysis with other Australian ST80 vanA VREfm isolates indicated that the 2011 expansion event was distinct and had limited spread to neighboring local health districts. Plasmid analysis showed multiple variants, traceable back to the 2009 isolate, indicating ongoing plasmid adaptation over time.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2022)
Article
Computer Science, Theory & Methods
Vikash Kumar Gupta, Balasubramaniam Jayaram
Summary: The article introduces the theory of importation algebra, which can introduce order on sets without assuming any order-theoretic properties, revealing new order-theoretic structures. It analyzes the sufficient conditions for importation posets to become lattices on [0, 1] and presents further research directions.
FUZZY SETS AND SYSTEMS
(2021)
Article
Immunology
Lan Chen, Wenxiu Ai, Ying Zhou, Chunyang Wu, Yinjuan Guo, Xiaocui Wu, Bingjie Wang, Lulin Rao, Yanlei Xu, Jiao Zhang, Liang Chen, Fangyou Yu
Summary: An outbreak of KPC-3-producing Enterobacterales infection occurred in China, with the involvement of clonal expansion and transmission across different species. These KPC-3 strains exhibited high virulence and readily developed resistance. Close monitoring of the spread of bla(KPC-3)-harboring plasmids and these strains is necessary in China and globally.
EMERGING INFECTIOUS DISEASES
(2022)
Article
Chemistry, Multidisciplinary
Cheng Chen, Yinsui Xu, Peter Oelschlaeger, Juergen Brem, Lu Liu, Dongmei Wang, Hongzhe Sun, Ke-Wu Yang
Summary: The emergence of multi-drug resistant pathogens co-expressing serine and metallo-carbapenemases significantly reduces the effectiveness of carbapenems. The first SeCN-derived dual inhibitor of serine and metallo-carbapenemases is reported, with IC50 values ranging from 0.0038 to 1.27 & mu;g mL(-1). The inhibitor forms covalent bonds with Cys221 of NDM-1 and Ser70 of KPC-2, achieving selective labelling and cross-class inhibition for carbapenemases. These findings provide a potential strategy to develop clinically useful dual inhibitors targeting serine and metallo-carbapenemases to combat superbugs.
CHEMICAL COMMUNICATIONS
(2023)
Article
Microbiology
Federico Nicola, Daniela Cejas, Francisco Gonzalez-Espinosa, Silvia Relloso, Fabian Herrera, Pablo Bonvehi, Jorgelina Smayevsky, Roque Figueroa-Espinosa, Gabriel Gutkind, Marcela Radice
Summary: This study describes an outbreak of ceftazidime-avibactam-resistant KPC variants of Klebsiella pneumoniae in a hospital in Argentina. The detection of these bacteria is challenging for the laboratory, and the history of previous infections and resistance testing may help raise awareness of this possibility.
MICROBIOLOGY SPECTRUM
(2022)
Article
Public, Environmental & Occupational Health
Alfred Lok Hang Lee, Eddie Chi Ming Leung, Ben Wai Hong Wong, Leo Chun Hei Wong, Yoyo Lok Yiu Wong, Rosana Ka Yin Hung, Sindy Sin Yee Ho, Viola Chi Ying Chow
Summary: MLST analysis confirmed that contamination of the laundry evaporative cooler and surrounding environment caused a polyclonal CRA hospital outbreak. Hospital laundry is an important area for infection control and outbreak investigations of CRA.
INFECTION CONTROL AND HOSPITAL EPIDEMIOLOGY
(2023)
Article
Public, Environmental & Occupational Health
Oyelola A. Adegboye, Adeshina Adekunle, Anton Pak, Ezra Gayawan, Denis H. Y. Leung, Diana P. Rojas, Faiz Elfaki, Emma S. McBryde, Damon P. Eisen
Summary: The study revealed significant impact of the Italian epidemic on the global spread of COVID-19, with correlations between travel patterns, arrival time, and case numbers. Changes in epicentres lead to shifts in transmission dynamics reflecting spatial proximities, resulting in varying levels of transmission risk in different regions.
TRAVEL MEDICINE AND INFECTIOUS DISEASE
(2021)
Article
Infectious Diseases
Mariana Castanheira, Timothy B. Doyle, Timothy D. Collingsworth, Helio S. Sader, Rodrigo E. Mendes
Summary: The study found an increasing trend in the rates of OXA-48-like-producing isolates over 3 years of global surveillance, with Klebsiella pneumoniae being the most common strain. Many isolates carried metallo-beta-lactamases, leading to resistance to most drugs tested. Further research is needed to help physicians understand treatment options for infections caused by these isolates.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2021)
Article
Environmental Sciences
Min Du, Shi Mo Zhang, Wei Jing Shang, Wen Xin Yan, Qiao Liu, Chen Yuan Qin, Min Liu, Jue Liu
Summary: This study analyzes the global epidemic of the 2022 monkeypox outbreak and assesses its importation risk into China. The study finds economic-related health disparities in the distribution of cases, disadvantageous to the rich. Additionally, it identifies countries or territories with high importation risk for China.
BIOMEDICAL AND ENVIRONMENTAL SCIENCES
(2022)
Article
Multidisciplinary Sciences
Christian Warmt, Carolin Kornelia Fenzel, Joerg Henkel, Frank Fabian Bier
Summary: This report demonstrates the direct labelling of RPA products with Cy5-dUTP during amplification process, eliminating the need for pre-labelled primers. The results show great potential as a flexible and promising tool with high specific and sensitive detection capabilities of target genes.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Alessandra Fiore, Leonie Zeitler, Marion Russier, Annette Gross, Maria-Kathrin Hiller, Joanne L. Parker, Luca Stier, Thomas Kocher, Simon Newstead, Peter J. Murray
Summary: This study uncovers an unrecognized link between IDO1 and cell survival mediated by KYN, which inhibits ferroptotic cell death. The findings highlight the need to re-evaluate the use of IDO1 inhibitors in cancer treatment.
Article
Chemistry, Multidisciplinary
Liqiang Hu, Runqiu Liu, Zheng Ma, Tao Yu, Ziyao Li, Yingqiu Zou, Chang Yuan, Fangfang Chen, Hexin Xie
Summary: A fluorogenic probe has been developed for the specific detection of IMP-1 beta-lactamase activity, featuring a unique trans-acetylamino cephalosporin as an enzymatic recognition moiety. This probe exhibits excellent selectivity to IMP-1 beta-lactamase over other beta-lactamases, with over 30-fold enhancement in fluorescence intensity upon activation by IMP-1 beta-lactamase, allowing for high sensitivity reporting of enzyme activity.
CHEMICAL COMMUNICATIONS
(2021)
Article
Chemistry, Organic
Takehiro Nishimura, Teppei Kawahara, Noritaka Kagaya, Yusuke Ogura, Hirosato Takikawa, Hikaru Suenaga, Masaatsu Adachi, Takatsugu Hirokawa, Takayuki Doi, Kazuo Shin-Ya
Summary: JBIR-155 was discovered as a novel specific class D beta-lactamase inhibitor from Streptomyces polymachus SoB100815Hv02. It showed inhibitory activity against the class D beta-lactamase with an IC50 value of 0.36 μM. The compound consists of a 6-oxabicyclo[3.2.0]heptan-7-one skeleton and a long unsaturated alkyl chain moiety.
Article
Microbiology
Stathis D. Kotsakis, Anastasia Lambropoulou, Georgios Miliotis, Eva Tzelepi, Vivi Miriagou, Leonidas S. Tzouvelekis
Summary: In the absence of a molecule that would collectively inhibit both metallo-β-lactamases and serine-reactive carbapenemases, containment of carbapenemase genes is the main strategy for addressing carbapenem resistance in hospitals. However, there is a need for cost-effective methods to rapidly detect carbapenemase-producing enterobacteria (CPE) to facilitate containment measures. This study developed a low-cost CPE detection method based on direct colorimetry, which showed high sensitivity and specificity in detecting CPE.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Juana Vega, Carlos H. Rodriguez, Ignacio Viscardi, Carlos Vay, Silvina Torres, Emilce Tabares, Angela Famiglietti, Marcela Nastro
Summary: We report a nosocomial outbreak of SME-4-producing S. marcescens in Buenos Aires city, which appears to be the first of its kind in South America.
REVISTA ARGENTINA DE MICROBIOLOGIA
(2023)
Article
Infectious Diseases
Samanta Freire, Teresa Grilo, Patrice Nordmann, Laurent Poirel, Marta Aires-de-Sousa
Summary: A rapid and reliable multiplex PCR was developed to simultaneously detect known plasmid-mediated fos genes conferring acquired resistance to fosfomycin. Our technique was tested on a collection of Escherichia coli isolates previously identified as bearing fosA, fosC, and fosL-like genes, demonstrating a sensitivity and specificity of 100%.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2023)
Article
Infectious Diseases
Mustafa Sadek, Remi Le Guern, Eric Kipnis, Philippe Gosset, Laurent Poirel, Rodrigue Dessein, Patrice Nordmann
Summary: This study reports the development of cefiderocol resistance among sequential Pseudomonas aeruginosa clinical isolates, without exposure to the drug. The resistance was driven by multifactorial mechanisms, including mutational changes in iron transporter proteins associated with drug uptake and overexpression of certain genes.
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
(2023)
Article
Infectious Diseases
Christophe Le Terrier, Patrice Nordmann, Mustafa Sadek, Laurent Poirel
Summary: This study evaluated the in vitro activity of cefepime/taniborbactam and cefepime/zidebactam against aztreonam/avibactam-resistant E. coli strains. The results showed that cefepime/taniborbactam had a resistance rate of 100% against these strains, while cefepime/zidebactam was effective in all cases. This suggests that cefepime/zidebactam combination could be a promising therapeutic option.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2023)
Article
Microbiology
Ahmad Ibrahim, Maxime Bouvier, Mustafa Sadek, Jean-Winoc Decousser, Laurent Poirel, Patrice Nordmann
Summary: Cefiderocol (FDC) is an effective siderophore cephalosporin against multidrug-resistant Gram-negative bacteria. The SuperFDC medium was developed to rapidly and accurately identify FDC-resistant pathogens, which is crucial for controlling their spread. The medium showed high sensitivity and specificity in detecting FDC-resistant isolates with various resistance mechanisms.
JOURNAL OF CLINICAL MICROBIOLOGY
(2023)
Article
Infectious Diseases
Jacqueline Findlay, Laurent Poirel, Patrice Nordmann
Summary: A study was conducted to select MEM-VAB resistant mutants and decipher the resistance mechanisms. The mutants showed reduced susceptibility to various antibiotics and had mutations in the ompK36 gene. Complementation with the wildtype ompK36 gene reduced the minimal inhibitory concentrations. Additionally, the bla KPC gene copy numbers were significantly increased in the resistant mutants.
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2023)
Letter
Infectious Diseases
Jacqueline Findlay, Juan Bosch Duran, Laurent Poirel, Patrice Nordmann
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2023)
Article
Microbiology
Samanta Freire, Teresa Grilo, Bruna Rodrigues, Rui Oliveira, Carla Esteves, Antonio Marques, Laurent Poirel, Marta Aires-de-Sousa
Summary: This study aimed to analyze the occurrence and characteristics of ESBL- and carbapenemase-producing E. coli among bivalves. Out of 522 collected bivalve samples, E. coli growth was observed in 39% of the samples. Nine samples (4.4%) were contaminated with ESBL producers and one sample (0.5%) was contaminated with a carbapenemase-producing K. pneumoniae. The presence of ESBL- and carbapenemase-producing Enterobacteriaceae in bivalves indicates that the marine environment could be a reservoir of critical bacterial pathogens, posing a potential risk to human health.
Article
Infectious Diseases
Otavio Hallal Ferreira Raro, Laurent Poirel, Maurine Tocco, Patrice Nordmann
Summary: The study aimed to evaluate whether subdosages of antibiotics administered in veterinary medicine could enhance plasmid transfer and resistance gene exchange in gut microbiota. The results showed that antibiotics in veterinary medicine may induce the transfer of plasmid-encoded resistance and contribute to the global spread of antibiotic resistance genes.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2023)
Editorial Material
Infectious Diseases
Jacqueline Findlay, Laurent Poirel, Patrice Nordmann
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2023)
Article
Microbiology
Clement Viguier, Maxime Bouvier, Mustafa Sadek, Auriane Kerbol, Laurent Poirel, Patrice Nordmann
Summary: This study develops a rapid culture-based test for the identification of Aztreonam-avibactam resistance among multidrug-resistant Enterobacterales. The test is rapid, highly sensitive, specific, easily interpretable, and can be easily implemented in routine laboratory practices.
JOURNAL OF CLINICAL MICROBIOLOGY
(2023)
Article
Infectious Diseases
Teresa Grilo, Samanta Freire, Bruno Miguel, Luis Nogueira Martins, Maria Favila Menezes, Patrice Nordmann, Laurent Poirel, Maria Jose Rego Sousa, Marta Aires-de-Sousa
Summary: This study evaluated the occurrence of plasmid-mediated fos genes among fosfomycin-resistant Escherichia coli isolates collected from patients in Lisbon, Portugal. The results showed a low prevalence of fosfomycin-resistant E. coli isolates, but 15% of these isolates carried transferable fosA genes, and a high rate of extended-spectrum beta-lactamase (ESBL) production was observed.
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2023)
