Article
Public, Environmental & Occupational Health
Suhaila A. Al-Sheboul, Ghina S. Al-Madi, Brent Brown, Wail A. Hayajneh
Summary: The prevalence of Klebsiella pneumoniae infections caused by antimicrobial resistance is increasing globally. In this study, K. pneumoniae producing ESBLs were investigated in samples from hematologic malignancy individuals, indicating a high prevalence of ESBL-producing K. pneumoniae.
JOURNAL OF EPIDEMIOLOGY AND GLOBAL HEALTH
(2023)
Review
Microbiology
Alain Philippon, Guillaume Arlet, Roger Labia, Bogdan I. Iorga
Summary: Class C β-lactamases exhibit molecular variability and can be categorized into two functional groups. These enzymes are encoded by chromosomal and inducible genes and are widely distributed among bacteria. Molecular identification is based on three catalytic motifs, and numerous conserved amino acid residues have been identified. The concept of natural ESACs is supported by the discovery of genetic modifications in pathogenic species and the emergence of resistance to carbapenems and inhibitors.
CLINICAL MICROBIOLOGY REVIEWS
(2022)
Article
Microbiology
Achhada Ujalkaur Avatsingh, Shilpa Sharma, Shilippreet Kour, Yukta Arora, Sheetal Sharma, Divya Joshi, Prem Prashant Chaudhary, Kahkashan Perveen, Mohab Amin Kamal, Nasib Singh
Summary: Antibiotic resistance in bacteria poses a serious threat to public health worldwide. Aquatic environments, including wastewater used for irrigation, contribute to the dissemination of antibiotic-resistant bacteria and genes, impacting human health and environmental sustainability. This study examines the prevalence of antibiotic resistance in Gram-negative bacteria isolated from irrigation-purpose wastewater in Himachal Pradesh, India. The findings highlight the presence of antibiotic-resistant and ESBL-positive bacterial isolates, emphasizing the need for ongoing monitoring and precautionary measures to manage wastewater and control environmental pollution.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Lizi Yin, Yuhong Gou, Yuyun Dai, Tao Wang, Kexin Gu, Ting Tang, Sajjad Hussain, Xiaoli Huang, Changliang He, Xiaoxia Liang, Gang Shu, Funeng Xu, Ping Ouyang
Summary: In recent years, infections caused by multidrug-resistant (MDR) bacteria have become a significant threat to global public health. This study investigated the potential of cinnamaldehyde as a synergistic agent with ceftriaxone sodium to combat MDR Salmonella. The results revealed that cinnamaldehyde enhanced the antibacterial activity of ceftriaxone sodium against MDR Salmonella both in vitro and in vivo, making it a promising adjuvant to prevent and treat MDR bacterial infections.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biophysics
Huma Fatima, Nikky Goel, Rajeshwari Sinha, Sunil Kumar Khare
Summary: Beta-lactam antibiotics are commonly used to treat bacterial infections, but bacterial resistance through beta-lactamase production is a major concern. Development of new strategies is essential to combat infections caused by resistant bacteria.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2021)
Article
Chemistry, Medicinal
Anka Lucic, Philip Hinchliffe, Tika R. Malla, Catherine L. Tooke, Jurgen Brem, Karina Calvopina, Christopher T. Lohans, Patrick Rabe, Michael A. McDonough, Timothy Armistead, Allen M. Orville, James Spencer, Christopher J. Schofield
Summary: Penems have shown potential as antibacterials and beta-lactamase inhibitors, but their clinical use is limited compared to carbapenems. Faropenem, with a C-2 tetrahydrofuran ring, is resistant to some beta-lactamases. Studies on reactions of faropenem with beta-lactamases demonstrate different outcomes, suggesting further research is needed for optimizing the interactions between penems and beta-lactamases.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Review
Food Science & Technology
Ryan Pearce, Beate Conrady, Luca Guardabassi
Summary: The objective of this study was to assess the prevalence and types of extended-spectrum β-lactamase (ESBL)-producing bacteria in retail seafood. A literature review found that the median prevalence of ESBL-contaminated products globally was 19.4%, with significantly higher rates in Asia compared to Europe and Africa. Among the analyzed isolates, 44.8% were ESBL-positive, with CTX-M being the predominant type (93.6%).
Article
Biochemistry & Molecular Biology
Manpreet Kaur, Yael Cohen, Elena Poverenov, Evgeni Eltzov
Summary: This study found that the combination of N-alkylaminated chitosan nanoparticles and conventional beta-lactam antibiotics has synergistic antibacterial potential against multidrug-resistant pathogens, and can effectively decrease the minimum inhibitory concentration of the antibiotics.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Microbiology
Tomefa E. Asempa, Hannah Bajor, Jessica H. Mullins, Janice Hartnett, David P. Nicolau
Summary: Research on identifying alternative growth media that better mimic host conditions is increasing. This study evaluated meropenem susceptibility against MBL-harboring Enterobacterales strains in urine, finding lower resistance compared to conventional broth. Factors influencing zinc concentrations in urine and their impact on antibiotic resistance warrant further investigation.
MICROBIOLOGY SPECTRUM
(2021)
Article
Biochemistry & Molecular Biology
Le Zhai, Ya Liu, Yue Jiang, Jian Xiao, Yi-Xue Wang, Yang Shi, Yi-Lin Zhang, Ke-Wu Yang
Summary: In this study, a novel vancomycin derivative V-b was constructed and shown to have significant antimicrobial effects against drug-resistant strains, enhancing the antibacterial activity of β-lactam antibiotics. Furthermore, V-b was able to enhance the antimicrobial activity of meropenem against clinical isolates and E. coli producing ImiS and CcrA.
Article
Microbiology
Georgi Merhi, Sara Amayri, Ibrahim Bitar, George F. Araj, Sima Tokajian
Summary: This study aimed to characterize multidrug resistant Enterobacter spp. and K. aerogenes isolates recovered from hospitalized patients in Lebanon. The isolates exhibited various resistance mechanisms and plasmid profiles. Multilocus sequence typing revealed the diversity within the isolates. Screening and continuous monitoring can effectively limit the dissemination of drug-resistant isolates in hospitalized patients.
MICROBIOLOGY SPECTRUM
(2023)
Article
Biotechnology & Applied Microbiology
Beenish Ehsan, Asma Haque, Muhammad Qasim, Aamir Ali, Yasra Sarwar
Summary: This study investigated antimicrobial resistance, specifically ESBLs and carbapenemases, in local uropathogenic Escherichia coli (UPEC) isolates. The results showed high resistance levels against certain antibiotics and a significant percentage of UPEC isolates producing ESBLs and carbapenemases. The study emphasizes the need for continuous surveillance of antimicrobial resistance and targeted antimicrobial therapy.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2023)
Article
Environmental Sciences
Folake Temitope Fadare, Anthony Ifeanyi Okoh
Summary: The effluents of wastewater treatment plants in the Eastern Cape Province of South Africa contain a high abundance of multidrug-resistant Enterobacteriaceae, indicating a high antibiotic pressure environment. This highlights the need to optimize current WWTP processes to reduce potential risks of environmental contamination and health hazards.
FRONTIERS IN ENVIRONMENTAL SCIENCE
(2021)
Article
Microbiology
Paula Kurittu, Banafsheh Khakipoor, Jari Jalava, Jari Karhukorpi, Annamari Heikinheimo
Summary: This study characterizes ESBL-producing E. coli from clinical samples in Finland using whole genome sequencing. It investigates potential zoonotic transmission routes and identifies successful AMR genes, bacterial sequence types, and plasmids. The results indicate the increasing prevalence of the ST131-C1-M27 clade in Europe and provide important information on the global spread of ESBL-producing E. coli.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Felipe Alberto-Lei, Carolina S. S. Nodari, Ana Paula Streling, Francisco O. O. Bessa-Neto, Andre V. Siqueira, Rodrigo Cayo, Ana Cristina Gales
Summary: In this study, a multidrug-resistant Pseudomonas juntendi clinical isolate carrying bla(BKC-1) and a class 1 integron containing multiple resistance gene cassettes was characterized. This finding confirms the ability of Pseudomonas putida group isolates to acquire antimicrobial resistance determinants and act as resistance reservoirs.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Biochemistry & Molecular Biology
Sonia Andre, Morgane Picard, Renaud Cezar, Florence Roux-Dalvai, Aurelie Alleaume-Butaux, Calaiselvy Soundaramourty, Andre Santa Cruz, Ana Mendes-Frias, Clarisse Gotti, Mickael Leclercq, Alexandre Nicolas, Alexandra Tauzin, Alexandre Carvalho, Carlos Capela, Jorge Pedrosa, Antonio Gil Castro, Lucy Kundura, Paul Loubet, Albert Sotto, Laurent Muller, Jean-Yves Lefrant, Claire Roger, Pierre-Geraud Claret, Sandra Duvnjak, Tu-Anh Tran, Gina Racine, Ouafa Zghidi-Abouzid, Pierre Nioche, Ricardo Silvestre, Arnaud Droit, Fabrizio Mammano, Pierre Corbeau, Jerome Estaquier
Summary: By comparing COVID-19 patients with healthy donors, the study found a strong correlation between T cell apoptosis and lymphopenia in severe cases. The plasma levels of soluble FasL (sFasL) and T cell surface expression of Fas/CD95 were positively correlated with T cell death and CD4 T cell counts. The study also identified modulations in the Bcl-2 family and demonstrated the potential of a pan-caspase inhibitor, Q-VD, to prevent T cell apoptosis and enhance Th1 transcripts.
CELL DEATH AND DIFFERENTIATION
(2022)
Article
Medicine, General & Internal
Jan Holubar, Jonathan Broner, Erik Arnaud, Olivier Halle, Thibault Mura, Benjamin Chambert, Albert Sotto, Camille Roubille, Cecile Gaujoux-Viala, Radjiv Goulabchand
Summary: Among 317 IUO patients, noninfectious inflammatory diseases were the major diagnoses, and F-18-FDG-PET/CT was found to be helpful in the diagnostic strategy. Polymyalgia rheumatica and giant cell arteritis were the top causes of noninfectious inflammatory diseases.
JOURNAL OF INTERNAL MEDICINE
(2022)
Article
Cell Biology
Sonia Andre, Marne Azarias da Silva, Morgane Picard, Aurelie Alleaume-Buteau, Lucy Kundura, Renaud Cezar, Calaiselvy Soudaramourty, Santa Cruz Andre, Ana Mendes-Frias, Alexandre Carvalho, Carlos Capela, Jorge Pedrosa, Antonio Gil Castro, Paul Loubet, Albert Sotto, Laurent Muller, Jean-Yves Lefrant, Claire Roger, Pierre-Geraud Claret, Sandra Duvnjak, Tu-Anh Tran, Ouafa Zghidi-Abouzid, Pierre Nioche, Ricardo Silvestre, Pierre Corbeau, Fabrizio Mammano, Jerome Estaquier
Summary: CD4 T-cell apoptosis affects the quality and quantity of humoral response in COVID-19 patients. Early in the infection, most patients exhibit a predominant IgA antibody response. Apoptotic CD4 T cells are inversely correlated with specific IgG antibody levels.
CELL DEATH & DISEASE
(2022)
Article
Immunology
Lucy Kundura, Renaud Cezar, Sonia Andre, Mauricio Campos-Mora, Claire Lozano, Thierry Vincent, Laurent Muller, Jean-Yves Lefrant, Claire Roger, Pierre-Geraud Claret, Sandra Duvnjak, Paul Loubet, Albert Sotto, Tu-Ahn Tran, Jerome Estaquier, Pierre Corbeau
Summary: T cell cytotoxicity, specifically the expression of perforin, may play a role in determining acute disease severity and the onset of long COVID in patients with SARS-CoV-2 infection. However, perforin expression is not correlated with disease severity or predictive of death. The percentage of perforin-positive T8 cells in the acute phase of the disease may predict the occurrence of long COVID.
FRONTIERS IN IMMUNOLOGY
(2022)
Letter
Infectious Diseases
Romaric Larcher, Paul Laffont-Lozes, Paul Loubet, Didier Laureillard, Tayma Naciri, Albert Sotto
CLINICAL MICROBIOLOGY AND INFECTION
(2023)
Article
Biochemistry & Molecular Biology
Chloe Magnan, Nour Ahmad-Mansour, Cassandra Pouget, Madjid Morsli, Sylvaine Huc-Brandt, Alix Pantel, Catherine Dunyach-Remy, Albert Sotto, Virginie Molle, Jean-Philippe Lavigne
Summary: This study performs whole-genome analysis on S. pettenkoferi strains and investigates their phylogenetic relationships, resistome, and virulome. Additionally, the study explores their ability to form biofilm, growth kinetics, and virulence in an in vivo zebrafish embryo infection model. The findings reveal genetic differences among strains and diversity in biofilm formation types.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Infectious Diseases
Cassandra Pouget, Alix Pantel, Catherine Dunyach-Remy, Chloe Magnan, Albert Sotto, Jean-Philippe Lavigne
Summary: Diabetic foot infections are a major public health problem. This study evaluated the in vitro activity of antibiotics against bacteria isolated from diabetic foot infections using a dynamic system. The combination of CWM and the BioFlux(TM) microfluidic system could help clinicians in managing chronic wounds.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2023)
Article
Hematology
Jean-Christophe Gris, Florence Guillotin, Taissa Pereira dos Santos, Mathias Chea, Paul Loubet, Didier Laureillard, Albert Sotto, Laurent Muller, Saber Davide Barbar, Claire Roger, Jean-Yves Lefrant, Boris Jung, Kada Klouche, Thibault Mura, Isabelle Quere, Antonia Perez-Martin
Summary: In this study, thrombin generation assays were performed on severe COVID-19 patients, and it was found that these assays could not predict the early or late survival rates of the patients, nor were they associated with thrombotic events. However, by detecting the changes in endogenous thrombin potential (ETP), it was possible to predict whether patients would need to be transferred to an intensive care unit in the future.
THROMBOSIS RESEARCH
(2023)
Article
Immunology
Romaric Larcher, Paul Laffont-Lozes, Claire Roger, Regine Doncesco, Celine Groul-Viaud, Aurelie Martin, Paul Loubet, Jean-Philippe Lavigne, Alix Pantel, Albert Sotto
Summary: This study described the use of last resort beta-lactam antibiotics in hospitalized patients and evaluated the microbiological and clinical outcomes. The results showed that the use of these antibiotics in real-life settings was a safe and efficient therapeutic option for severe infections caused by Gram-negative bacteria with Difficult-to-Treat Resistance.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Public, Environmental & Occupational Health
Koko Barrigah-Benissan, Jerome Ory, Claire Simon, Paul Loubet, Aurelie Martin, Jean-Paul Beregi, Jean-Philippe Lavigne, Albert Sotto, Romaric Larcher
Summary: This study aimed to assess the clinical factors associated with peripherally inserted central catheter (PICC)-related bloodstream infections (PR-BSI), with a focus on clinical symptoms. The results showed that fever and chills were factors associated with the occurrence of PR-BSI.
ANTIMICROBIAL RESISTANCE AND INFECTION CONTROL
(2023)
Article
Infectious Diseases
Paul Laffont-Lozes, Didier Laureillard, Paul Loubet, Robin Stephan, Myriam Chiaruzzi, Edouard Clemmer, Aurelie Martin, Claire Roger, Laurent Muller, Pierre-Geraud Claret, Radjiv Goulabchand, Clarisse Roux, Jean-Philippe Lavigne, Albert Sotto, Romaric Larcher
Summary: We conducted a prospective cohort study to assess the factors associated with mortality in patients with SARS-CoV-2 pneumonia treated with tocilizumab. Our results showed that age, Charlson index, WHO-CPS, and early administration of tocilizumab were independently associated with mortality. There was no significant difference in mortality between patients treated with tocilizumab and standard of care alone, but better survival was observed in patients who received tocilizumab within 48 hours of hospital admission.
Article
Infectious Diseases
Tayma Naciri, Boris Monnin, Alix Pantel, Claire Roger, Jean-Marie Kinowski, Paul Loubet, Jean-Philippe Lavigne, Albert Sotto, Romaric Larcher
Summary: This study compared the outcomes of beta-lactam allergic (BLA) patients and non-beta-lactam allergic (NBLA) patients with intra-abdominal infections (IAI). The results showed that BLA patients treated without beta-lactam had the same treatment outcomes as NBLA patients treated with beta-lactam.
Article
Infectious Diseases
Jerome Ory, Quang Le Minh, Hung Phan Tien, Vinh Vu Hai, Elodie Careno, Tatiana Price, Alexandre Andrieux, Julien Crouzet, Catherine Dunyach-Remy, Didier Laureillard, Jean-Philippe Lavigne, Albert Sotto
Summary: This study examined the changes in surgical site infection rates before and after the implementation of antibiotic stewardship programs and infection control measures in a Vietnamese tertiary care hospital. The results showed a decrease in surgical site infection rates after the implementation of these programs, although the difference was not statistically significant. Comprehensive infection control education and policies are needed in Vietnamese hospitals to prevent surgical site infections.
Article
Microbiology
Aurelie Martin, Paul Loubet, Florian Salipante, Paul Laffont-Lozes, Julien Mazet, Jean-Philippe Lavigne, Nicolas Cellier, Albert Sotto, Romaric Larcher
Summary: This study describes the clinical characteristics and treatment outcomes of patients with enterococcal bone and joint infections and identifies factors associated with treatment failure. The study found that enterococcal BJIs have poor prognosis, with a treatment failure rate of 39%. Coinfection with Staphylococcus epidermidis and the presence of local signs of inflammation at the time of diagnosis were identified as risk factors for treatment failure.
Article
Urology & Nephrology
Franck Bruyere, Yoann Conan, Maxime Vallee, Christophe Gaborit, Hugo Crespin, Joseph-Alain Ruimy, Albert Sotto, Leslie Grammatico-Guillon
Summary: This study identified age and uropathy as independent predictors of abnormal ultrasound in women treated with community-onset acute pyelonephritis.
WORLD JOURNAL OF UROLOGY
(2022)