Article
Immunology
Diana Fernandez-Rodriguez, Claudia Adriana Colin-Castro, Melissa Hernandez-Duran, Luis Esau Lopez-jacome, Rafael Franco-Cendejas
Summary: This study found a relatively high proportion of Staphylococcus epidermidis small colony variants in prosthetic joint infections, which were associated with difficulties in achieving remission. Small colony variants showed higher resistance rates to trimethoprim/sulfamethoxazole and lower resistance rates to levofloxacin.
MICROBES AND INFECTION
(2021)
Review
Chemistry, Inorganic & Nuclear
Jordi Zamarreno Beas, Marco A. M. Videira, Ligia M. Saraiva
Summary: Haem b and sirohaem are two important prosthetic groups in bacterial proteins, synthesized through complex pathways with divergences at the formation of uroporphyrinogen III. The biosynthesis of these tetrapyrroles involves unstable or potentially hazardous intermediates and end-products, requiring tight control of cellular metabolic fluxes.
COORDINATION CHEMISTRY REVIEWS
(2022)
Editorial Material
Plant Sciences
Jawahar Singh, Oswaldo Valdes-Lopez
Summary: NCR247 plays a vital role in the differentiation of endocytosed bacteria into nitrogen-fixing bacteroids. A recent study shows that NCR247 is crucial for the uptake of iron, a mineral nutrient essential for nitrogenase activity. Additionally, its unique ability to sequester haem suggests potential applications in human health.
TRENDS IN PLANT SCIENCE
(2023)
Article
Chemistry, Medicinal
Sandeep Lohan, Anastasia G. Konshina, Roman G. Efremov, Innokentiy Maslennikov, Keykavous Parang
Summary: A series of short helical cationic peptides were designed and synthesized to have broad-range bactericidal activity and selectivity toward bacterial cells. The lead peptide 8b showed broad-spectrum activity against both Gram-positive and Gram-negative bacteria, as well as selectivity towards prokaryotic cells. The peptide's rapid membranolytic action was confirmed through assays and microscopy.
JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Article
Infectious Diseases
Jalin A. Jordan, Richard E. Lenski, Kyle J. Card
Summary: Antibiotic resistance is an increasing concern, and this study focuses on the fitness and cost of resistance in ampicillin-resistant mutants. The findings highlight the importance of comparative studies and reveal the nuanced processes behind antibiotic resistance phenotypes.
Article
Biophysics
Emanuelle D. Freitas, Rogerio A. Rataglioli, Josephine Oshodi, Marisa M. Beppu
Summary: The coronavirus pandemic has highlighted the importance of bioactive materials in mitigating viral infections. Bioinspired antimicrobial peptides (AMPs) have emerged as suitable building blocks for antimicrobial coatings due to their versatile design and environmentally friendly features. This review discusses the advances and opportunities in using AMPs to create virucidal coatings, including the fundamental characteristics of peptide structure, advances in coating medical devices, and the role of different materials in designing antiviral coatings.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2022)
Article
Immunology
Mohammed Y. Y. Behairy, Mohamed A. A. Soltan, Muhammad Alaa Eldeen, Jawaher A. A. Abdulhakim, Maryam M. M. Alnoman, Mohamed M. M. Abdel-Daim, Hassan Otifi, Saleh M. M. Al-Qahtani, Mohamed Samir A. Zaki, Ghadi Alsharif, Sarah Albogami, Ibrahim Jafri, Eman Fayad, Khaled M. M. Darwish, Sameh S. S. Elhady, Refaat A. A. Eid
Summary: This study conducted a comprehensive analysis to identify damaging missense SNPs for hBD-2 and LL-37 through multiple in silico approaches. The analysis revealed the presence of three damaging SNPs in hBD-2, which were predicted to decrease the stability of hBD-2 and impact its structure. Docking-coupled molecular dynamics simulation analysis showed compromised binding affinity for hBD-2 SNPs towards the SARS-CoV-2 spike domain. The findings provide a model for identifying COVID-19 susceptible patients and guiding personalized diagnostic and management protocols.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Aetas Amponnawarat, Chalatip Chompunud Na Ayudhya, Hydar Ali
Summary: Pseudomonas aeruginosa infections are difficult to treat due to biofilm formation and antibiotic resistance, but a lipidated HDP mimetic called murepavadin has shown antibacterial activity against multi-drug-resistant strains. Murepavadin activates human MCs via MRGPRX2 and murine MCs via MrgprB2, potentially contributing to bacterial clearance and wound healing.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Food Science & Technology
Jiaju Qiao, Mengjiao Zhu, Yun Fan, Zhaoxin Lu, Fengxia Lv, Haizhen Zhao, Xiaomei Bie
Summary: This paper investigated the properties of cold-induced small colony variants (SCVs) of Staphylococcus aureus, including antibiotic resistance, biofilm formation, and virulence. The results showed that SCVs had slow growth, increased resistance to antibiotics, and enhanced biofilm formation under specific gene regulation.
Review
Microbiology
Greater Kayode Oyejobi, Xiaoxu Zhang, Dongyan Xiong, Faith Ogolla, Heng Xue, Hongping Wei
Summary: Phage treatment shows potential for bacterial infections, but bacterial resistance to phage is a challenge. Research on phage resistance and coevolutionary dynamics between phage and bacteria is crucial for successful phage therapy.
CRITICAL REVIEWS IN MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Pablo Yubero, Juan F. Poyatos
Summary: This study examines the difficulties in assessing the fitness cost of complex pleiotropic mutations and proposes a statistical framework incorporating direct effects and other molecular variables as predictors. Using mutations in Escherichia coli RNA polymerase as a model, it identifies certain genes as more suitable predictors for determining costs despite pleiotropy, which has implications for understanding robustness in biological systems.
MOLECULAR BIOLOGY AND EVOLUTION
(2021)
Article
Multidisciplinary Sciences
Connie Fung, Lisa M. Fraser, Gabriel M. Barron, Matthew B. Gologorsky, Samantha N. Atkinson, Elias R. Gerrick, Michael Hayward, Jennifer Ziegelbauer, Jessica A. Li, Katherine F. Nico, Miles D. W. Tyner, Leila B. DeSchepper, Amy Pan, Nita H. Salzman, Michael R. Howitt
Summary: Succinate produced by commensal protist T. mu stimulates tuft cells, leading to type 2 immunity. Microbial-derived succinate increases Paneth cell numbers and alters the AMP landscape in the small intestine. Tuft cells utilize SUCNR1 and succinate sensing to modulate bacterial homeostasis.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Plant Sciences
Ka Zhang, Fei Liu, Zhixin Wang, Chenjian Zhuo, Kaining Hu, Xiaoxia Li, Jing Wen, Bin Yi, Jinxiong Shen, Chaozhi Ma, Tingdong Fu, Jinxing Tu
Summary: In this study, it was found that oilseed rape enhances its resistance against Sclerotinia infection through a conserved MAPK cascade-mediated phosphorylation of BnWRKY33. Additionally, the induction of BnaA03.WRKY28 and BnaA09.VQ12 in the later stages of infection suppresses BnWRKY33 expression but promotes branching growth. These findings highlight the adaptability of oilseed rape in response to lethal pathogens, as it modulates its defense-response strength while balancing growth.
Article
Chemistry, Medicinal
Bo-Yuan Shen, Ming-Ming Wang, Shuai-Min Xu, Chen Gao, Meng Wang, Sen Li, Maxwell Ampomah-Wireko, Sheng-Cong Chen, Da-Chao Yan, Shangshang Qin, En Zhang
Summary: Bacterial resistance is a growing threat to public health, and the development of novel antibacterial strategies is critical. In this study, a series of chalcone-alkyl-lysine compounds were developed, mimicking the structure and properties of cationic antimicrobial peptides. Compound 6d showed potent antibacterial activity against various bacteria, including MRSA and NDM-positive isolates. The mechanism of action involved the destruction of bacterial cell membranes, leading to bacterial death. The compound also exhibited low toxicity in cell experiments.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Review
Chemistry, Medicinal
Jose Maria Garcia-Beltran, Marta Arizcun, Elena Chaves-Pozo
Summary: Aquaculture production is increasing, but infectious diseases pose a threat. Antimicrobial peptides (AMPs) from photosynthetic marine organisms have potential as alternatives to antibiotics in aquaculture due to their defense mechanisms and additional activities. This study reviews the knowledge about AMPs from photosynthetic marine organisms and evaluates their suitability for aquaculture.
Article
Immunology
Karin Hjort, Pontus Juren, Juan Carlos Toro, Sven Hoffner, Dan Andersson, Linus Sandegren
Summary: Whole-genome sequencing of Mycobacterium tuberculosis from a single patient over 9 years of disease and treatment revealed that antibiotic treatment was the main driver of sequence evolution, leading to stepwise accumulation of resistance mutations to various antibiotics. The study highlighted the dynamic nature of M. tuberculosis infection, population structure, and resistance evolution, emphasizing the importance of rapid antibiotic susceptibility tests in combating this pathogen.
JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Microbiology
Clara Atterby, Marie Nykvist, Ulrika Lustig, Dan Andersson, Josef D. Jarhult, Linus Sandegren
Summary: Exposure to sub-MIC levels of ciprofloxacin can lead to enrichment of Escherichia coli with reduced susceptibility to ciprofloxacin in wildlife, highlighting the potential impact of antibiotic pollution on the environment. The study found that in vivo selection of gyrA mutants occurred in mallards during exposure to ciprofloxacin concentrations found in aquatic environments, with resistance plasmids readily transferring between strains in the intestines of the mallards. Ultimately, the findings underscore the importance of reducing antibiotic contamination to curb the spread of resistance.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Infectious Diseases
Jessica Howard-Anderson, Michelle Davis, Alexander M. Page, Chris W. Bower, Gillian Smith, Jesse T. Jacob, Dan Andersson, David S. Weiss, Sarah W. Satola
Summary: This study investigated the prevalence of colistin heteroresistance in carbapenem-resistant Pseudomonas aeruginosa (CRPA) and its association with clinical outcomes. The results showed that colistin heteroresistance was present in up to 26% of CRPA patients, but it did not have a significant impact on 90-day mortality.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2022)
Article
Immunology
Greta Zaborskyte, Erik Wistrand-Yuen, Karin Hjort, Dan I. Andersson, Linus Sandegren
Summary: Medical device-related biofilms are a major cause of hospital-acquired infections. The 3D-printed device (FlexiPeg) presented in this study is a robust and easy-to-use tool for studying biofilms, with versatile applications.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Food Science & Technology
Konstantinos Koutsoumanis, Ana Allende, Avelino Alvarez-Ordonez, Declan Bolton, Sara Bover-Cid, Marianne Chemaly, Robert Davies, Alessandra De Cesare, Lieve Herman, Friederike Hilbert, Roland Lindqvist, Maarten Nauta, Giuseppe Ru, Marion Simmons, Panagiotis Skandamis, Elisabetta Suffredini, Dan Andersson, Vasileios Bampidis, Johan Bengtsson-Palme, Damien Bouchard, Aude Ferran, Maryline Kouba, Secundino Lopez Puente, Marta Lopez-Alonso, Soren Saxmose Nielsen, Alena Pechova, Mariana Petkova, Sebastien Girault, Alessandro Broglia, Beatriz Guerra, Matteo Lorenzo Innocenti, Ernesto Liebana, Gloria Lopez-Galvez, Paola Manini, Pietro Stella, Luisa Peixe
Summary: The study assessed the specific concentrations of trimethoprim in non-target feed for food-producing animals, as well as the antimicrobial concentrations in feed that have an effect on growth promotion. The FARSC model was applied for addressing antimicrobial resistance, and further studies were recommended to supply more diverse and complete data.
Article
Food Science & Technology
Konstantinos Koutsoumanis, Ana Allende, Avelino Alvarez-Ordonez, Declan Bolton, Sara Bover-Cid, Marianne Chemaly, Robert Davies, Alessandra De Cesare, Lieve Herman, Friederike Hilbert, Roland Lindqvist, Maarten Nauta, Giuseppe Ru, Marion Simmons, Panagiotis Skandamis, Elisabetta Suffredini, Dan I. Andersson, Vasileios Bampidis, Johan Bengtsson-Palme, Damien Bouchard, Aude Ferran, Maryline Kouba, Secundino Lo'pez Puente, Marta Lopez-Alonso, Soren Saxmose Nielsen, Alena Pechova, Mariana Petkova, Sebastien Girault, Alessandro Broglia, Beatriz Guerra, Matteo Lorenzo Innocenti, Ernesto Liebana, Gloria Lopez-Galvez, Paola Manini, Pietro Stella, Luisa Peixe
Summary: EFSA, in collaboration with EMA, assessed the specific concentrations of colistin and other antimicrobials in feed for their impact on antimicrobial resistance and growth promotion, using the FARSC model and scientific publication data, recommending further studies to fill data gaps.
Article
Food Science & Technology
Konstantinos Koutsoumanis, Ana Allende, Avelino Alvarez-Ordonez, Declan Bolton, Sara Bover-Cid, Marianne Chemaly, Robert Davies, Alessandra De Cesare, Lieve Herman, Friederike Hilbert, Roland Lindqvist, Maarten Nauta, Giuseppe Ru, Marion Simmons, Panagiotis Skandamis, Elisabetta Suffredini, Dan Andersson, Vasileios Bampidis, Johan Bengtsson-Palme, Damien Bouchard, Aude Ferran, Maryline Kouba, Secundino Lopez Puente, Marta Lopez-Alonso, Soren Saxmose Nielsen, Alena Pechova, Mariana Petkova, Sebastien Girault, Alessandro Broglia, Beatriz Guerra, Matteo Lorenzo Innocenti, Ernesto Liebana, Gloria Lopez-Galvez, Paola Manini, Pietro Stella, Luisa Peixe
Summary: EFSA collaborated with EMA to assess specific concentrations of florfenicol and thiamphenicol in non-target feed, as well as their effects on antimicrobial resistance and growth promotion. The FARSC model was used for assessment, with florfenicol's FARSC estimated but thiamphenicol's calculation pending further experimental data. Data from literature reviews were used to determine growth promotion effects, but no suitable data was available for thiamphenicol. Further studies and data generation were recommended to address uncertainties and gaps in the assessment.
Article
Food Science & Technology
Konstantinos Koutsoumanis, Ana Allende, Avelino Alvarez-Ordonez, Declan Bolton, Sara Bover-Cid, Marianne Chemaly, Robert Davies, Alessandra De Cesare, Lieve Herman, Friederike Hilbert, Roland Lindqvist, Maarten Nauta, Giuseppe Ru, Marion Simmons, Panagiotis Skandamis, Elisabetta Suffredini, Dan Andersson, Vasileios Bampidis, Johan Bengtsson-Palme, Damien Bouchard, Aude Ferran, Maryline Kouba, Secundino Lopez Puente, Marta Lopez-Alonso, Soren Saxmose Nielsen, Alena Pechova, Mariana Petkova, Sebastien Girault, Alessandro Broglia, Beatriz Guerra, Matteo Lorenzo Innocenti, Ernesto Liebana, Gloria Lopez-Galvez, Paola Manini, Pietro Stella, Luisa Peixe
Summary: The study assessed specific concentrations of tilmicosin, tylosin, and tylvalosin in feed for food-producing animals, and their impact on bacterial resistance and growth promotion. The Feed Antimicrobial Resistance Selection Concentration (FARSC) model was used for the assessment of antimicrobial resistance, while scientific publications were utilized for growth promotion evaluation. Additional studies are recommended to fill the data gaps for tylvalosin.
Article
Food Science & Technology
Konstantinos Koutsoumanis, Ana Allende, Avelino Alvarez-Ordonez, Declan Bolton, Sara Bover-Cid, Marianne Chemaly, Robert Davies, Alessandra De Cesare, Lieve Herman, Friederike Hilbert, Roland Lindqvist, Maarten Nauta, Giuseppe Ru, Marion Simmons, Panagiotis Skandamis, Elisabetta Suffredini, Dan Andersson, Vasileios Bampidis, Johan Bengtsson-Palme, Damien Bouchard, Aude Ferran, Maryline Kouba, Secundino Lopez Puente, Marta Lopez-Alonso, Soren Saxmose Nielsen, Alena Pechova, Mariana Petkova, Sebastien Girault, Alessandro Broglia, Beatriz Guerra, Matteo Lorenzo Innocenti, Ernesto Liebana, Gloria Lopez-Galvez, Paola Manini, Pietro Stella, Luisa Peixe
Summary: The study assessed the specific concentrations of four tetracyclines in feed for food-producing animals, as well as their effects on bacterial resistance and growth promotion. The FARSC model and data from scientific publications were used for the assessment, highlighting uncertainties and data gaps. Further studies were recommended to provide more diverse and complete data for calculation of the FARSC for these antimicrobials.
Article
Food Science & Technology
Konstantinos Koutsoumanis, Ana Allende, Avelino Alvarez-Ordonez, Declan Bolton, Sara Bover-Cid, Marianne Chemaly, Robert Davies, Alessandra De Cesare, Lieve Herman, Friederike Hilbert, Roland Lindqvist, Maarten Nauta, Giuseppe Ru, Marion Simmons, Panagiotis Skandamis, Elisabetta Suffredini, Dan Andersson, Vasileios Bampidis, Johan Bengtsson-Palme, Damien Bouchard, Aude Ferran, Maryline Kouba, Secundino Lopez Puente, Marta Lopez-Alonso, Soren Saxmose Nielsen, Alena Pechova, Mariana Petkova, Sebastien Girault, Alessandro Broglia, Beatriz Guerra, Matteo Lorenzo Innocenti, Ernesto Liebana, Gloria Lopez-Galvez, Paola Manini, Pietro Stella, Luisa Peixe
Summary: EFSA, in collaboration with EMA, assessed specific concentrations of amprolium in non-target feed and specific antimicrobial concentrations in feed for growth promotion. The assessment was based on the Feed Antimicrobial Resistance Selection Concentration (FARSC) model, but a lack of data prevented a conclusion on antimicrobial resistance. Data from scientific publications were used to report levels of amprolium in feed affecting growth promotion.
Review
Microbiology
Roderich Roemhild, Tobias Bollenbach, Dan I. Andersson
Summary: This review discusses the importance of using combination antibiotics to enhance treatment efficacy and combat resistance evolution. While several promising strategies have been proposed, a deeper understanding of the physiological responses and genetic mechanisms underlying antibiotic interactions and their clinical applicability is still lacking.
NATURE REVIEWS MICROBIOLOGY
(2022)
Article
Immunology
Po-Cheng Tang, Olle Eriksson, Josefin Sjogren, Nikos Fatsis-Kavalopoulos, Johan Kreuger, Dan I. Andersson
Summary: Biofilms are a crucial mode of growth for bacteria, but how antibiotic resistance emerges and is selected in biofilms is poorly understood. This study developed and validated a microfluidic chip (Brimor) for studying the dynamics of antibiotic-resistant bacteria in biofilms. The chip was found to be easy to use and a relevant model for studying the selection of antibiotic resistance in bacterial biofilms, providing valuable insights into this area of research.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Microbiology
Karin Hjort, Elin Fermer, Po-Cheng Tang, Dan I. Andersson
Summary: The use and misuse of antibiotics have led to the selection of difficult-to-treat resistant bacteria. This study compared the selection of antibiotic-resistant E. coli strains during planktonic and biofilm growth. The results showed that resistant mutants were selected at sub-minimal inhibitory concentrations (MICs) in both growth conditions, emphasizing the significance of low antibiotic concentrations in driving the emergence and enrichment of resistant bacteria.
Article
Biology
Arianne M. Babina, Serhiy Surkov, Weihua Ye, Jon Jerlstrom-Hultqvist, Marten Larsson, Erik Holmqvist, Per Jemth, Dan Andersson, Michael Knopp
Summary: The origins and evolution of small proteins with diverse physiological roles remain unclear. Recent genomic sequence analyses suggest that new functions encoded by small open reading frames (sORFs) may emerge de novo from noncoding sequences. This study shows that randomly generated sORFs can rescue Escherichia coli cells by upregulating hisB expression and that they bind to the 5' end regulatory region of the his operon mRNA, promoting structural rearrangements of the RNA for increased hisB expression. This provides experimental evidence that small proteins with selective benefits can originate from the expression of nonfunctional sequences.
Article
Biology
Ramith R. Nair, Dan I. Andersson
Summary: The dynamics of nitrofurantoin resistance selection in Escherichia coli is significantly affected by interspecies interactions with Bacillus subtilis. The presence of B. subtilis slows down the selection for resistant E. coli mutants by producing extracellular compounds, particularly the YydF peptide. This study highlights the importance of considering interspecies interactions in understanding and predicting the evolution of antibiotic resistance.
COMMUNICATIONS BIOLOGY
(2023)