Article
Infectious Diseases
Julie Gorham, Fabio S. Taccone, Maya Hites
Summary: Obesity is associated with increased risks of comorbidities, postoperative infections, and nosocomial infections. The pharmacokinetics and pharmacodynamics of antibiotics are affected by obesity, leading to increased risks of antibiotic therapy failure and toxicity. Precision dosing of antibiotics using therapeutic drug monitoring may improve the management of critically ill obese patients with infections.
Article
Microbiology
Suresh Ambati, Emma C. Ellis, Tuyetnhu Pham, Zachary A. Lewis, Xiaorong Lin, Richard B. Meagher
Summary: The use of Dectin-2-targeted liposomes to deliver amphotericin B has shown significant efficacy in reducing fungal burden at infection sites in the lungs and prolonging survival in mice with pulmonary aspergillosis. This targeted delivery system has the potential to improve treatment outcomes for various fungal diseases by enhancing the efficacy of antifungal drugs at lower doses.
Article
Chemistry, Medicinal
Ruben A. G. van Eerden, Esther Oomen-de Hoop, Aad Noordam, Ron H. J. Mathijssen, Stijn L. W. Koolen
Summary: Therapeutic drug monitoring (TDM) is important in oncology for small molecule kinase inhibitors (SMKIs) to ensure optimal drug exposure, and extrapolating randomly taken drug concentrations to trough concentrations using elimination half-life is a feasible method for multiple SMKIs. Timing of blood withdrawals and specific extrapolation methods may vary for different SMKIs.
Review
Pharmacology & Pharmacy
Bruno Charlier, Albino Coglianese, Federica De Rosa, Ugo de Grazia, Francesca Felicia Operto, Giangennaro Coppola, Amelia Filippelli, Fabrizio Dal Piaz, Viviana Izzo
Summary: Epilepsy is a complex neurological disorder with various syndromes and treatments, including the use of antiseizure medications (ASMs) that act on synaptic levels. Monitoring ASMs levels in biological fluids is crucial to minimize risks, and measuring the free drug fraction is increasingly important for better correlation with pharmacological and toxicological effects. Validated methodologies exist for separating and quantifying different drug forms, but further validation studies are needed to connect pharmacokinetic monitoring with clinical outcomes in real-world settings.
Article
Chemistry, Medicinal
Domenico Umberto De Rose, Iliana Bersani, Maria Paola Ronchetti, Fiammetta Piersigilli, Sara Cairoli, Andrea Dotta, Amit Desai, Laura Lynn Kovanda, Bianca Maria Goffredo, Cinzia Auriti
Summary: To investigate the pharmacokinetics of micafungin in neonates, researchers analyzed the data of 53 newborns treated with micafungin. The results showed that neonates had higher clearance and shorter half-life of micafungin compared to older patients. Furthermore, micafungin could reach therapeutic levels in the cerebrospinal fluid when administered at doses ranging from 8 to 15 mg/kg/day.
Article
Pharmacology & Pharmacy
Sonja E. Zapke, Stefan Willmann, Scott-Oliver Grebe, Kristin Menke, Petra A. Thurmann, Sven Schmiedl
Summary: The study compared the performance of a PBPK model for cyclosporine and found that 86% of predicted cyclosporine trough levels deviated less than twofold from observed values. The model did not fully cover the high intra-individual variability of observed cyclosporine levels. Considering clinical and additional patient-related factors may improve the model's performance.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Federica Pigliasco, Alessia Cafaro, Raffaele Simeoli, Sebastiano Barco, Alberto Magnasco, Maura Faraci, Gino Tripodi, Bianca Maria Goffredo, Giuliana Cangemi
Summary: The study developed a new LC-MS/MS micro-method for simultaneous quantification of aciclovir and ganciclovir, with potential applications in pediatric medicine. The assay was accurate and reproducible in both plasma and dried plasma spots, showing comparable results between the two sample types.
Article
Multidisciplinary Sciences
Masaichi Kato, Ken Sugimoto, Kentaro Ikeya, Ryosuke Takano, Ai Matsuura, Takahiro Miyazu, Natsuki Ishida, Satoshi Tamura, Shinya Tani, Mihoko Yamade, Yasushi Hamaya, Moriya Iwaizumi, Satoshi Osawa, Takahisa Furuta, Hiroyuki Hanai
Summary: This study investigated the correlation between ADA monitoring and real-world IBD clinical outcomes, finding that ADA continuity was associated with AAA positivity and serum ADA levels. Significant differences were observed between endoscopic remission and non-remission groups based on serum ADA levels.
Article
Chemistry, Analytical
Stefano Tartaggia, Anna Meneghello, Ottavia Bellotto, Ariana Soledad Poetto, Martina Zanchetta, Bianca Posocco, David Bunka, Federico Polo, Giuseppe Toffoli
Summary: This study developed a detection assay for the anticancer drug imatinib using a ssDNA aptamer, demonstrating excellent selectivity and stability for therapeutic drug monitoring (TDM). By utilizing surface plasmon resonance (SPR) analysis, a quantitative assay in the concentration range of 400 to 6000 ng mL(-1) was established, showing high precision and accuracy. The method, validated according to regulatory guidelines, offers a feasible aptasensor for TDM in patients receiving imatinib treatment.
Article
Medical Laboratory Technology
Michael Vogeser
Summary: Therapeutic drug monitoring plays a crucial role in medicine, yet it is often overlooked in laboratory medicine. A goal for laboratory medicine in the 21st century should be enabling clinicians to verify the presence of the right drug in the right patient with appropriate blood concentrations for each compound.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Dongxiao Ma, Zhengchao Ji, Haiwei Cao, Jing Huang, Lei Zeng, Lei Yin
Summary: This study developed a detection method based on HPLC/MS3 strategy for quantifying carbamazepine in human plasma. The method was validated for various parameters and showed good accuracy, precision, and selectivity. It was successfully used for monitoring the blood concentration of epilepsy patients after carbamazepine treatment.
Article
Chemistry, Medicinal
Yingjie Qi, Guangxuan Liu
Summary: An ultra-performance liquid chromatography-tandem mass spectrometry method was developed and validated for simultaneous therapeutic drug monitoring and clinical pharmacokinetic antipsychotic drugs, providing significant information for future research and showing a simple, reliable, specific, and suitable technique for TDM and pharmacokinetic studies.
DRUG DESIGN DEVELOPMENT AND THERAPY
(2021)
Article
Biochemistry & Molecular Biology
Ying Li, Lu Meng, Yinling Ma, Yajing Li, Xiaoqing Xing, Caihui Guo, Zhanjun Dong
Summary: This study developed a simple and effective UPLC-MS/MS method for simultaneous quantification of osimertinib, aumolertinib, and furmonertinib in human plasma, which can be used for TDM. The method is sensitive, reliable, and easy to use, potentially reducing the incidence of adverse effects.
Article
Chemistry, Analytical
Zhenjie Ye, Lingjie Wu, Xiaoying Zhang, Yingying Hu, Ling Zheng
Summary: A method for simultaneous quantification of multi-targeted tyrosine kinase inhibitors sorafenib, lenvatinib, and apatinib in human plasma was developed in this study for therapeutic drug monitoring in hepatocellular carcinoma patients. The method showed satisfactory specificity, precision, accuracy, matrix effects, extraction recovery, and stability, providing a sensitive, rapid, and simple approach for drug analysis.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2021)
Article
Pediatrics
Yue Li, Hong-Li Guo, Yuan-Yuan Zhang, Na Dong, Ya-Hui Hu, Jing Chen, Xiao-Peng Lu, Feng Chen
Summary: This study investigated the efficacy and safety of lacosamide in Chinese children with epilepsy, both as monotherapy and adjunctive treatment. The study found that children with higher body weight and older age had lower plasma concentration ratios of lacosamide to daily dose, and complex drug interactions were observed between lacosamide and other antiepileptic drugs.
FRONTIERS IN PEDIATRICS
(2022)
Article
Pharmacology & Pharmacy
Julie Hias, Lorenz Van der Linden, Karolien Walgraeve, Matthias Gijsen, Paola Mian, Birgit C. P. Koch, Karel Allegaert, Pieter Annaert, Jos Tournoy, Isabel Spriet
Summary: This study evaluated the pharmacokinetics of two oral paracetamol formulations in elderly patients aged 80 years and older. Results showed large interindividual differences in pharmacokinetic parameters of paracetamol in frail inpatients, with female sex and higher total serum bilirubin concentrations associated with paracetamol exposure.
BRITISH JOURNAL OF CLINICAL PHARMACOLOGY
(2022)
Review
Pharmacology & Pharmacy
Anne Smits, Pieter Annaert, Giacomo Cavallaro, Pieter A. J. G. De Cock, Saskia N. de Wildt, Jenny M. Kindblom, Florian B. Lagler, Carmen Moreno, Paula Pokorna, Michiel F. Schreuder, Joseph F. Standing, Mark A. Turner, Benedetto Vitiello, Wei Zhao, Annelie-Martina Weingberg, Raffaella Willmann, John van den Anker, Karel Allegaert
Summary: Developmental pharmacology focuses on the impact of maturation on drug metabolism and effects in pediatric patients, providing insights into drug development strategies and highlighting the importance of considering developmental pharmacodynamics. This paper summarizes current knowledge, identifies remaining gaps, and suggests innovative approaches for studying drug disposition and effects in children.
BRITISH JOURNAL OF CLINICAL PHARMACOLOGY
(2022)
Review
Pharmacology & Pharmacy
Elin Ngo, Olav Spigset, Angela Lupattelli, Alice Panchaud, Pieter Annaert, Karel Allegaert, Hedvig Nordeng
Summary: Current data on the use of antihistamines during breastfeeding and risks to the breastfed infants are insufficient, further human studies are needed; most product labels recommend avoiding the use of antihistamines during breastfeeding.
BASIC & CLINICAL PHARMACOLOGY & TOXICOLOGY
(2022)
Article
Infectious Diseases
Matthias Gijsen, Omar Elkayal, Pieter Annaert, Ruth Van Daele, Philippe Meersseman, Yves Debaveye, Joost Wauters, Erwin Dreesen, Isabel Spriet
Summary: This study evaluated the pharmacokinetic/pharmacodynamic (PK/PD) target attainment of meropenem in critically ill patients with preserved or increased renal function. The results showed poor target attainment, with renal function being the only significant covariate.
INFECTION AND DRUG RESISTANCE
(2022)
Article
Pharmacology & Pharmacy
Ruben Faelens, Nicolas Luyckx, Dirk Kuypers, Thomas Bouillon, Pieter Annaert
Summary: Before developing a precision dosing tool for tacrolimus, the tool's performance was evaluated through simulation. The results showed that the tool improved the probability of target attainment and dosing precision.
CPT-PHARMACOMETRICS & SYSTEMS PHARMACOLOGY
(2022)
Article
Pharmacology & Pharmacy
Caroline S. Foo, Rana Abdelnabi, Suzanne J. F. Kaptein, Xin Zhang, Sebastiaan ter Horst, Raf Mols, Leen Delang, Joana Rocha-Pereira, Lotte Coelmont, Pieter Leyssen, Kai Dallmeier, Valentijn Vergote, Elisabeth Heylen, Laura Vangeel, Arnab K. Chatterjee, Pieter P. Annaert, Patrick F. Augustijns, Steven De Jonghe, Dirk Jochmans, Mieke Gouwy, Seppe Cambier, Jennifer Vandooren, Paul Proost, Christine van Laer, Birgit Weynand, Johan Neyts
Summary: Nelfinavir, an HIV protease inhibitor, shows antiviral activity against SARS-CoV-2 and can improve lung pathology induced by the virus in hamsters. It enhances the number and functionality of neutrophils and promotes their migration. Another combination of HIV protease inhibitors, Lopinavir/Ritonavir, also has a similar protective effect in this model.
ANTIVIRAL RESEARCH
(2022)
Meeting Abstract
Biochemistry & Molecular Biology
Nina Nauwelaerts, Thomas Bouillon, Neel Deferm, Bart Lammens, Pieter Annaert
Meeting Abstract
Biochemistry & Molecular Biology
Kristof De Vos, Pieter Annaert
Article
Biochemistry & Molecular Biology
Axelle Cooreman, Anne Caufriez, Andres Tabernilla, Raf Van Campenhout, Kaat Leroy, Prashant Kadam, Julen Sanz Serrano, Bruna dos Santos Rodrigues, Pieter Annaert, Mathieu Vinken
Summary: This study investigated the effects of several drugs proposed for treating COVID-19 on Cx43 hemichannels. The results showed that a combination of lopinavir and ritonavir, as well as remdesivir, reduced Cx43 mRNA levels, but none of the drugs affected Cx43 protein expression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Infectious Diseases
Erwin Dreesen, Matthias Gijsen, Omar Elkayal, Pieter Annaert, Yves Debaveye, Joost Wauters, Mats O. Karlsson, Isabel Spriet
Summary: The study aimed to determine an optimized ceftriaxone dosage regimen based on the probability of developing augmented renal clearance (ARC) in critically ill patients with community-acquired pneumonia (CAP). It was found that critically ill patients with CAP who have a high probability of developing ARC may benefit from twice-daily 2 g ceftriaxone dosing for achieving adequate exposure on the next day.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2022)
Article
Pediatrics
Elif Keles, Pia Wintermark, Floris Groenendaal, Noor Borloo, Anne Smits, Annouschka Laenen, Djalila Mekahli, Pieter Annaert, Suzan Sahin, Mehmet Yekta Oncel, Valerie Chock, Didem Armangil, Esin Koc, Malcolm R. Battin, Adam Frymoyer, Karel Allegaert
Summary: There is a large variability in kidney function and injury in neonates with neonatal encephalopathy treated with therapeutic hypothermia. Using serum creatinine centiles over postnatal age may provide more valuable information in these neonates. Understanding postnatal age-related patterns enables clinicians to better assess kidney function and tailor treatment.
Article
Pharmacology & Pharmacy
Nina Nauwelaerts, Michael Ceulemans, Neel Deferm, An Eerdekens, Bart Lammens, Yeghig Armoudjian, Kristel Van Calsteren, Karel Allegaert, Loes de Vries, Pieter Annaert, Anne Smits
Summary: The study found low concentrations of sildenafil and bosentan in human milk, with low daily doses ingested by the nursing infant. However, the Relative Infant Dose calculated for both medications were within safe range. The mother reported that the infant's general health was optimal until the sampling days.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Oncology
Getahun Befirdu Abza, Jemal Hussien Ahmed, Abdu Adem Yesufe, Edom Seife, Mengistu Erkie, Isabel Spriet, Legese Chelkeba, Pieter Annaert
Summary: Hepatitis B virus (HBV) is an important risk factor for hepatocellular carcinoma (HCC). The clinical conditions and survival of patients with HCC in Ethiopia are poor. Patients who received antiviral therapy had better survival outcomes than those who did not. Therefore, it is crucial to increase vaccination coverage and access to antiviral drugs against hepatitis to reduce the devastation of HCC.
Article
Pharmacology & Pharmacy
Yeghig Armoudjian, Qi Lin, Bart Lammens, Johan Van Daele, Pieter Annaert
Summary: The IMI project ConcePTION was launched to fill the knowledge gap of using medicines during pregnancy and lactation. A high-throughput, robust, and reliable liquid chromatography tandem mass spectrometry method was developed and validated to determine the concentrations of amoxicillin in a large number of minipig plasma and milk samples.
JOURNAL OF PHARMACOLOGICAL AND TOXICOLOGICAL METHODS
(2023)
Article
Pharmacology & Pharmacy
Julia Macente, Nina Nauwelaerts, Francesca M. Russo, Jan Deprest, Karel Allegaert, Bart Lammens, Rodolfo Hernandes Bonan, Jessica M. Turner, Sailesh Kumar, Andrea Diniz, Frederico S. Martins, Pieter Annaert
Summary: This study aims to use physiologically-based pharmacokinetic modeling to predict the maternal dose required for therapeutic fetal exposure in the treatment of congenital diaphragmatic hernia.
FRONTIERS IN PHARMACOLOGY
(2023)