Article
Infectious Diseases
Anna Vilaro, Elena Novell, Vicens Enrique-Tarancon, Jordi Balielles, Lourdes Migura-Garcia, Lorenzo Fraile
Summary: Antimicrobial susceptibility testing is important for antimicrobial stewardship, but limited resources often restrict the number of drugs that can be tested for each antimicrobial family. This study examined the minimal inhibitory concentration for various bacterial strains collected in Spain, and found that there were generally no significant associations between MIC patterns for different antimicrobial families. However, some drug-bug combinations showed high correlation, indicating that MIC values from one representative drug could be indicative of the whole antimicrobial family.
Article
Microbiology
J. Goransson, M. Sundqvist, E. Ghaderi, J. G. Lisby, Y. Molin, E. Eriksson, S. Carlsson, A. Cederlof, L. Ellis, J. Melin
Summary: This article introduces the application of the ASTar system in the treatment of bloodstream infections and evaluates its performance. The results show that the ASTar system has high consistency and accuracy when compared to the standard method.
JOURNAL OF CLINICAL MICROBIOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Daniel Spencer, Yuetao Li, Yiling Zhu, J. Mark Sutton, Hywel Morgan
Summary: A novel test has been developed to assess the susceptibility of bacteria to antibiotics by measuring changes in impedance, providing a rapid determination of electrical minimum inhibitory concentration (eMIC). This test accurately predicts sensitivity or resistance of bacterial strains to various antibiotics within a fraction of the time compared to traditional methods.
Article
Infectious Diseases
Marco Bottinelli, Michele Gastaldelli, Micaela Picchi, Arianna Dall'Ora, Lorena Cristovao Borges, Ana Sofia Ramirez, Andrea Matucci, Salvatore Catania
Summary: The susceptibility of Mycoplasma gallisepticum to commonly used drugs has changed over a ten-year period, with a comeback to susceptibility observed for certain drugs.
Article
Veterinary Sciences
Maria D. Vegas Comitre, Stefano Cortellini, Marc Cherlet, Mathias Devreese, Beatrice B. Roques, Alain Bousquet-Melou, Pierre-Louis Toutain, Ludovic Pelligand
Summary: This study investigated the pharmacokinetics of amoxicillin-clavulanic acid in healthy and critically ill dogs, finding significant differences in drug clearance between the two populations. The results suggested potential adjustments to dosing regimens in clinical settings for sick dogs.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Fisheries
Claudio D. Miranda, Rodrigo Rojas, Sergio Contreras-Lynch, Alonso Vega
Summary: The study found a moderate correlation between antibiotics amoxicillin and florfenicol, while highly significant correlations were detected for antibiotics oxytetracycline, oxolinic acid, flumequine, and enrofloxacin, indicating a tight association between MIC and disk values.
Review
Veterinary Sciences
Longfei Zhang, Hongbing Xie, Yongqiang Wang, Hongjuan Wang, Jianhe Hu, Gaiping Zhang
Summary: Pharmacokinetic/pharmacodynamic (PK/PD) integration models are used to investigate the antimicrobial activity characteristics of drugs targeting pathogenic bacteria through comprehensive analysis of the interactions between PK and PD parameters. PK/PD models have been widely applied in the development of new drugs, optimization of the dosage regimen, and prevention and treatment of drug-resistant bacteria.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Spectroscopy
Mohammad Javad Jafari, Mohsen Golabi, Thomas Ederth
Summary: The use of infrared attenuated total reflection (IR-ATR) provides a simple method for assessing bacterial susceptibility to antibiotics by monitoring bacterial metabolic activities. Principal component analysis can quickly discriminate antimicrobial resistance based on obtained spectra.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2024)
Article
Biochemical Research Methods
Andrew A. Heller, Morgan K. Geiger, Dana M. Spence
Summary: This study designed a two-compartment model using 3D printing technology to mimic the pharmacokinetics of molecules in the human body, which can be used to evaluate the pharmacodynamics of antibiotics.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Microbiology
Franziska Guetgemann, Anja Mueller, Yury Churin, Franziska Kumm, Ann Sophie Braun, Min Yue, Tobias Eisenberg, Monika Entorf, Thomas Peters, Corinna Kehrenberg
Summary: This study aimed to develop a method for harmonized broth microdilution antimicrobial susceptibility testing (AST) of Mycoplasma bovis. The SP4 broth was found to be a suitable medium for AST, and consistent minimum inhibitory concentration (MIC) values were obtained. The validation of M. bovis DSM 22781(T) as a future quality control (QC) strain is recommended.
JOURNAL OF CLINICAL MICROBIOLOGY
(2023)
Article
Infectious Diseases
Dusten T. Rose, Alexander Moskhos, Arya Wibisono, Kelly R. Reveles
Summary: This study evaluated the impact of using MicroScan and VITEK 2 automated systems to determine vancomycin MIC values on the treatment of MRSA bacteremia. The results showed that the MicroScan group had a higher rate of vancomycin alternative therapy use compared to the VITEK 2 group, while the VITEK 2 group had a shorter median hospital stay.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Microbiology
Yi Ye, Ye Tian, Yueyue Kong, Jiawei Ma, Guangzhi Shi
Summary: Coagulase-negative staphylococci (CoNS) are the main pathogens in health care-associated ventriculitis and meningitis. The study found that the rates of resistance to primary antimicrobial agents are high and those to high-level agents, including vancomycin, are low. The trends of resistance rates are acceptable, especially for high-level agents, although long-term and continuous monitoring is necessary. The clinical outcomes of neurosurgical adults with coagulase-negative staphylococci-related health care-associated ventriculitis and meningitis are acceptable after treatment with vancomycin. Therefore, according to the antimicrobial susceptibility and clinical practice, vancomycin will be safe to treat coagulase-negative staphylococci-related health care-associated ventriculitis and meningitis.
MICROBIOLOGY SPECTRUM
(2022)
Article
Infectious Diseases
Laura M. Koeth, Jeanna M. DiFranco-Fisher, Nicole E. Scangarella-Oman
Summary: This study determined the effect of urine on the in vitro activity of gepotidacin and levofloxacin against relevant bacteria. The impact of urine on the MICs of gepotidacin and levofloxacin was minimal and not inclusive of all strains.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2023)
Review
Pharmacology & Pharmacy
Cornelia B. Landersdorfer, Roger L. Nation
Summary: This article discusses the importance of pharmacokinetic and pharmacodynamic indices of antibiotics in drug development and clinical applications. It highlights the limitations of these indices and proposes a model-based approach to improve antibiotic development and dosing in patients by incorporating factors that can influence treatment outcomes.
FRONTIERS IN PHARMACOLOGY
(2021)
Review
Infectious Diseases
Ina Gajic, Jovana Kabic, Dusan Kekic, Milos Jovicevic, Marina Milenkovic, Dragana Mitic Culafic, Anika Trudic, Lazar Ranin, Natasa Opavski
Summary: Antimicrobial resistance poses a significant threat to global public health. Accurate and rapid detection of antibiotic resistance and appropriate treatment methods are crucial for controlling its emergence. However, traditional testing methods are time-consuming and costly, highlighting the need for innovative approaches.
Article
Infectious Diseases
Elena Carrara, Alessia Savoldi, Laura J. Piddock, Francois Franceschi, Sally Ellis, Mike Sharland, Adrian John Brink, Patrick N. A. Harris, Gabriel Levy-Hara, Anusha Rohit, Constantinos Tsioutis, Hiba Zayyad, Christian Giske, Margherita Chiamenti, Damiano Bragantini, Elda Righi, Anna Gorska, Evelina Tacconelli
Summary: The optimal treatment for carbapenem-resistant Gram-negative bacteria infections varies globally, with dual combination therapy being the preferred treatment scheme. However, there is disagreement among clinicians regarding the definition of 'combination therapy.'
CLINICAL MICROBIOLOGY AND INFECTION
(2022)
Article
Infectious Diseases
Anna Akerlund, Alexandros Petropoulos, Karin Malmros, Thomas Tangden, Christian G. Giske
Summary: This study assessed blood culture diagnostics in the Nordic countries and compared them with a previous survey conducted in Sweden in 2013. The results showed that although improvements have been made, such as implementing satellite incubators and rapid species identification and susceptibility testing, limited opening hours and availability of clinical microbiologists are still confining the benefits of these changes.
CLINICAL MICROBIOLOGY AND INFECTION
(2022)
Review
Microbiology
Christian G. Giske, John Turnidge, Rafael Canton, Gunnar Kahlmeter
Summary: EUCAST is an international committee focused on susceptibility testing, aiming to harmonize clinical breakpoints in Europe and beyond. Its activities include collecting MIC distribution data, developing software, identifying epidemiological cutoff values, and creating testing methods. EUCAST's decisions are subject to public consultation, except for breakpoints of new antimicrobial agents.
JOURNAL OF CLINICAL MICROBIOLOGY
(2022)
Article
Pediatrics
Frida Oldendorff, Agnes Linner, Mikael Finder, Peter Eisenlauer, Malin Kjellberg, Christian G. Giske, Viveka Nordberg
Summary: This case report describes a fatal meningitis caused by ESBL-E. coli ST 1193 in a newborn, highlighting the importance of clinical knowledge about multidrug-resistant clones.
FRONTIERS IN PEDIATRICS
(2022)
Article
Biochemistry & Molecular Biology
Xiangning Bai, Aswathy Narayanan, Magdalena Skagerberg, Rafael Cena-Diez, Christian G. Giske, Kristoffer Stralin, Anders Sonnerborg
Summary: This study reveals correlations between the upper respiratory tract microbiome and clinical outcomes in COVID-19 patients, particularly a strong association between Moraxella lincolnii and vitamin K2 biosynthesis pathways with better clinical results.
Article
Infectious Diseases
Tanya Babich, Pontus Naucler, John Karlsson Valik, Christian G. Giske, Natividad Benito, Ruben Cardona, Alba Rivera, Celine Pulcini, Manal Abdel Fattah, Justine Haquin, Alasdair Macgowan, Sally Grier, Bibiana Chazan, Anna Yanovskay, Ronen Ben Ami, Michal Landes, Lior Nesher, Adi Zaidman-Shimshovitz, Kate McCarthy, David L. Paterson, Evelina Tacconelli, Michael Buhl, Susanna Mauer, Jesus Rodriguez-Bano, Marina de Cueto, Antonio Oliver, Enrique Ruiz de Gopegui, Angela Cano, Isabel Machuca, Monica Gozalo-Marguello, Luis Martinez-Martinez, Eva M. Gonzalez-Barbera, Iris Gomez Alfaro, Miguel Salavert, Bojana Beovic, Andreja Saje, Manica Mueller-Premru, Leonardo Pagani, Virginie Vitrat, Diamantis Kofteridis, Maria Zacharioudaki, Sofia Maraki, Yulia Weissman, Mical Paul, Yaakov Dickstein, Leonard Leibovici, Dafna Yahav
Summary: This study aimed to evaluate the impact of short versus long antibiotic courses on the outcomes of patients with Pseudomonas aeruginosa bacteremia. The results showed that a 6-10 day antibiotic course was as effective as longer courses in terms of survival and recurrence, with shorter therapy associated with reduced length of stay and less drug discontinuation.
INFECTIOUS DISEASES AND THERAPY
(2022)
Article
Biology
Katja Wallander, Martin Vondracek, Christian G. Giske
Summary: Clinicians face challenges in identifying bacterial causes of bone and joint infections, which can lead to poor clinical outcomes. This study shows that analyzing multiple samples per patient with 16S rDNA sequencing can help diagnose postoperative bone and joint infections, especially in cases where cultures fail. The method can provide additional diagnostic value for patients receiving antibiotic treatment and should be considered as a complement to traditional culturing methods.
BMC RESEARCH NOTES
(2022)
Article
Infectious Diseases
Chaitanya Tellapragada, Karin Andersson Ydsten, Anders Ternhag, Christian G. Giske
Summary: This study investigated the concordance between the Unyvero Hospitalized Pneumonia application and quantitative culture in detecting bacterial pathogens from consecutive lower respiratory tract specimens. The false positive rate of the HPN application was 29% and 10% in the two evaluation approaches, respectively. Additional pathogens detected by the HPN application were confirmed by culture positivity in previous or subsequent samples.
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
(2022)
Article
Immunology
Jasper Van Heuverswyn, John Karlsson Valik, Suzanne Desiree van der Werff, Pontus Hedberg, Christian Giske, Pontus Naucler
Summary: This cohort study found that delays in appropriate antimicrobial treatment were associated with increased 30-day mortality after 12 hours from blood culture collection, but not at 1, 3, and 6 hours.
CLINICAL INFECTIOUS DISEASES
(2023)
Article
Infectious Diseases
Alaa Atamna, Ili Margalit, Gida Ayada, Tanya Babich, Pontus Naucler, John Karlsson Valik, Christian G. Giske, Natividad Benito, Ruben Cardona, Alba Rivera, Celine Pulcini, Manal Abdel Fattah, Justine Haquin, Alasdair Macgowan, Bibiana Chazan, Anna Yanovskay, Ronen Ben Ami, Michal Landes, Lior Nesher, Adi Zaidman-Shimshovitz, Kate McCarthy, David L. Paterson, Evelina Tacconelli, Michael Buhl, Susanna Mauer, Jesus Rodriguez-Bano, Marina de Cueto, Antonio Oliver, Enrique Ruiz de Gopegui, Angela Cano, Isabel Machuca, Monica Gozalo-Marguello, Luis Martinez-Martinez, Eva M. Gonzalez-Barbera, Iris Gomez Alfaro, Miguel Salavert, Bojana Beovic, Andreja Saje, Manica Mueller-Premru, Leonardo Pagani, Virginie Vitrat, Diamantis Kofteridis, Maria Zacharioudaki, Sofia Maraki, Yulia Weissman, Mical Paul, Yaakov Dickstei, Dafna Yahav
Summary: This study aimed to evaluate outcomes of P. aeruginosa bacteremia among older adults (> 80 years) and found that disease severity, place of acquisition, and background conditions were associated with mortality, while antimicrobial regimen was not. Therefore, preventive efforts and early diagnosis might be beneficial for improving outcomes in older adults with P. aeruginosa bacteremia.
Article
Infectious Diseases
Gabrielle Froberg, Florian P. Maurer, Erja Chryssanthou, Louise Fernstrom, Hanaa Benmansour, Samira Boarbi, Anne Torunn Mengshoel, Peter Michael Keller, Miguel Viveiros, Diana Machado, Margaret M. Fitzgibbon, Simone Mok, Jim Werngren, Daniela Maria Cirillo, Fernando Alcaide, Hanne-Leena Hyyrylainen, Alexandra Aubry, Sonke Andres, Darshaalini Nadarajan, Erik Svensson, John Turnidge, Christian G. Giske, Gunnar Kahlmeter, Emmanuelle Cambau, Jakko van Ingen, Thomas Schon
Summary: The study evaluated the minimum inhibitory concentration (MIC) distributions of non-tuberculous mycobacteria (NTM) to establish antimicrobial susceptibility testing (AST) breakpoints. MIC distributions for drugs against Mycobacterium avium complex (MAC) and Mycobacterium abscessus (MAB) were collected from 12 laboratories. Epidemiological cut-off values (ECOFFs) and tentative ECOFFs (TECOFFs) were determined, highlighting the need for further method refinement in establishing clinical breakpoints for NTM.
CLINICAL MICROBIOLOGY AND INFECTION
(2023)
Article
Biology
Flora Mikaeloff, Marco Gelpi, Rui Benfeitas, Andreas D. Knudsen, Beate Vestad, Julie Hogh, Johannes R. Hov, Thomas Benfield, Daniel Murray, Christian G. Giske, Adil Mardinoglu, Marius Troseid, Susanne D. Nielsen, Ujjwal Neogi, Niel Hens
Summary: Multiomics technologies were used to stratify and characterize the metabolic risk profile in people living with HIV (PWH) on antiretroviral therapy. Through network analysis and similarity network fusion (SNF), three groups of PWH were identified: healthy-like, mild at-risk, and severe at-risk. PWH in the severe at-risk group had a high incidence of metabolic syndrome and dysregulated metabolic traits, despite having higher CD4(+) T-cell counts. The microbiome profile differed between the healthy-like group and the at-risk groups, potentially contributing to systemic inflammation and cardiometabolic risk.
Article
Health Care Sciences & Services
Karin Rystedt, Katarina Hedin, Mia Tyrstrup, Gunilla Skoog-Stahlgren, Charlotta Edlund, Christian G. Giske, Ronny Gunnarsson, Paer-Daniel Sundvall
Summary: The aim of this study was to compare the effectiveness of rapid antigen detection test (RADT) and throat culture for group A streptococci (GAS) among patients recently treated with penicillin V. The results showed 91% agreement between RADT and culture at a follow-up visit within 21 days. The study concludes that RADT carries a low risk of missing the presence of GAS.
SCANDINAVIAN JOURNAL OF PRIMARY HEALTH CARE
(2023)
Article
Infectious Diseases
Kk Sriram, Moa S. Wranne, Tsegaye Sewunet, Elina Ekedahl, Maarten Coorens, Teerawit Tangkoskul, Visanu Thamlikitkul, Christian G. Giske, Fredrik Westerlund
Summary: This study demonstrates the use of optical DNA mapping (ODM) to characterize plasmids carrying the colistin resistance gene mcr-1. The researchers successfully identified plasmids encoding mcr-1 and demonstrated the ability of ODM to identify resistance gene sites in small plasmids. They also propose a cut-and-stretch assay using surface-functionalized cover slips and a basic microscope to identify the mcr-1 gene in plasmid samples. These techniques could be valuable in identifying plasmids carrying antibiotic resistance in hospitals, particularly in low- and middle-income countries.
JAC-ANTIMICROBIAL RESISTANCE
(2022)
Article
Public, Environmental & Occupational Health
Refath Farzana, Gote Swedberg, Christian G. Giske, Badrul Hasan
Summary: This study investigated the molecular epidemiology of carbapenem-resistant Acinetobacter baumannii in a hospital in Bangladesh. The results showed the presence of multidrug-resistant strains in both clinical and environmental samples, with similar clone clustering patterns, indicating widespread transmission of the strains between the two environments.
INFECTION PREVENTION IN PRACTICE
(2022)