Article
Infectious Diseases
Rima Shrestha, Courtney L. Luterbach, Weixiao Dai, Lauren Komarow, Michelle Earley, Gregory Weston, Erica Herc, Jesse T. Jacob, Robert Salata, Darren Wong, Deverick Anderson, Kirsten B. Rydell, Cesar A. Arias, Liang Chen, David van Duin
Summary: Community-acquired carbapenem-resistant Enterobacterales (CA-CRE) pose a significant threat in hospitalized patients. This study identified specific genetic markers associated with CA-CRE and found that Klebsiella pneumoniae was the most common CRE species.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2022)
Article
Biochemistry & Molecular Biology
Elias Eger, Stefan E. Heiden, Karsten Becker, Andrea Rau, Katharina Geisenhainer, Evgeny A. Idelevich, Katharina Schaufler
Summary: This study conducted both genomic and phenotypic experiments to analyze two K. pneumoniae isolates causing severe multiple site infection in a human patient. It revealed that the isolates belong to sequence type 420, suggesting they are of the hypervirulent pathotype, and identified the insertion of a hypervirulence plasmid in the bacterial chromosome mediated by a new IS5 family sub-group IS903 insertion sequence.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Microbiology
Hazrat Bilal, Gaojian Zhang, Tayyab Rehman, Jianxion Han, Sabir Khan, Muhammad Shafiq, Xuegang Yang, Zhongkang Yan, Xingyuan Yang
Summary: The study revealed the presence of various carbapenemases-producing K. pneumoniae strains in clinical samples from Pakistan, with a high prevalence of those carrying the bla(NDM-1) gene. These strains with different sequence types also showed some variation in cluster analysis. This study also identified IncX3 plasmids carrying the bla(NDM-1) gene for the first time in Pakistan, suggesting a potential new vehicle for disseminating bla(NDM-1) among Enterobacteriaceae due to its high transferability rate.
Article
Microbiology
Guoye Liu, Huimin Qian, Jingwen Lv, Benshun Tian, Changjun Bao, Hong Yan, Bing Gu
Summary: The study revealed an increasing trend of antimicrobial resistance in nontyphoidal Salmonella in Jiangsu, China, particularly towards colistin. Plasmid-mediated resistance genes play a significant role in these bacteria, with mcr-1-positive strains predominantly found in children under 7 years old.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Infectious Diseases
Suraj Shukla, Siddhi Desai, Ashutosh Bagchi, Pushpendra Singh, Madhvi Joshi, Chaitanya Joshi, Jyoti Patankar, Geeti Maheshwari, Ekadashi Rajni, Manali Shah, Devarshi Gajjar
Summary: The prevalence of beta-lactamases, especially carbapenemases, in Klebsiella pneumoniae isolates from India was evaluated in this study. The most prevalent sequence types (ST) among Indian isolates were ST231, ST147, ST2096, and ST14. The gene bla(ampH) was found to be the most prevalent, followed by bla(CTX-M-15) and bla(TEM-1). The study also identified ST16 and ST14 as potential high-risk clones in India, capable of producing both carbapenemases and ESBL genes.
Article
Microbiology
Xiaoli Wang, Bin Tang, Guitian Liu, Meng Wang, Jingyong Sun, Ruoming Tan, Tingting Pan, Jieming Qu, Jialin Liu, Hong-Yu Ou, Hongping Qu
Summary: Klebsiella pneumoniae poses a critical challenge to clinical and public health due to the global spread of conjugative and nonconjugative plasmids associated with carbapenem-resistant, hypervirulent, and even dual-resistant and hypervirulent strains. This study identified a clinically significant CRKP strain and demonstrated the high conjugation ability of its IncN3 plasmid, which can mobilize nonconjugative virulence or resistance plasmids. The findings suggest a need for further monitoring of the transmission of pathogenicity and genetic evolution of carbapenem-resistant and hypervirulent K. pneumoniae strains.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Qinqin Liu, Zhiyun Guo, Gang Zhu, Ning Li, Guanchen Bai, Meijie Jiang
Summary: In this study, a multidrug-resistant strain of Klebsiella pneumoniae (K. pneumoniae) was isolated from a patient in a tertiary hospital in Tai'an city, China. The strain carried a plasmid carrying the mobile colistin resistance (MCR) genes, posing a significant threat to clinical treatment. Whole-genome sequencing and conjugation tests showed that the plasmid could be horizontally transmitted between bacteria. This study provides important data for the treatment and surveillance of K. pneumoniae infection.
Article
Microbiology
Qiwei Chen, Lizhang Liu, Xiaofang Hu, Xu Jia, Xiaowei Gong, Youjun Feng, Man Huang
Summary: The study focuses on the diversity of KPC-2-producing plasmids in highly transmissible ST11 clones of carbapenem-resistant K. pneumoniae (CRKP). The researchers report a clinical case of a rare ST437 CRKP isolate, K186, which produces KPC-2. Whole-genome sequencing revealed three distinct resistance plasmids, one of which, pK186_KPC, is a small IncN-type plasmid of 26,012bp. The study further suggests that the carbapenem-resistant pK186_KPC might have arisen from the integration of ancestral IncN and IncFII plasmids.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Patricia Perez-Palacios, Ana Gual-de-Torrella, Mercedes Delgado-Valverde, Jesus Oteo-Iglesias, Carmen Hidalgo-Diaz, Alvaro Pascual, Felipe Fernandez-Cuenca
Summary: The study found that biofilm formation can affect the spread of OXA-48-Kpn, especially in high-risk clones. The presence of biocides reduced the fold changes in CF-BF/CF-PK in HR isolates, while it had minimal effect on non-HR isolates.
MICROBIOLOGICAL RESEARCH
(2022)
Article
Genetics & Heredity
Lisa Neffe, Taya L. Forde, Katarina Oravcova, Ute Koehler, Wilfried Bautsch, Juergen Tomasch, Susanne Haeussler
Summary: This study used whole-genome sequencing to describe the population structure of ESBL-producing Escherichia coli and Klebsiella pneumoniae isolates from a German community hospital. The study found a high proportion of globally disseminated ST131 strains among E. coli isolates, while K. pneumoniae isolates showed wider diversity of STs. Genetic analysis revealed multiple introductions of ST131 strains and a regional clustering of clinical isolates. Patient-to-patient transmission was rare, suggesting a primarily community and regional acquisition of ESBL-producing strains.
MICROBIAL GENOMICS
(2022)
Review
Pharmacology & Pharmacy
Arnold Lee, Yvette N. Lamb, Matt Shirley
Summary: Delafloxacin, a fluoroquinolone antibacterial, has demonstrated efficacy in treating community-acquired pneumonia (CAP) and skin infections. It is as effective as moxifloxacin in treating CAP and may be more effective in patients with asthma or COPD. Delafloxacin is well-tolerated and has a low rate of adverse events in CAP patients.
Article
Medicine, General & Internal
Ying Zhou, Yang Yang, Xiaobin Li, Dongxing Tian, Wenxiu Ai, Weiwen Wang, Bingjie Wang, Barry N. Kreiswirth, Fangyou Yu, Liang Chen, Xiaofei Jiang
Summary: In this study, the native CRISPR system of K. pneumoniae was used to successfully cure multidrug-resistant IncFII plasmids through conjugation-mediated delivery of the CRISPR-Cas3 system. The work demonstrated that CRISPR-mediated plasmid curing could re-sensitize drug-resistant K. pneumoniae to multiple antibiotics.
Article
Infectious Diseases
Yann Mouanga Ndzime, Richard Onanga, Roland Fabrice Kassa Kassa, Michelle Bignoumba, Pierre Philippe Mbehang Nguema, Amahani Gafou, Romeo Wenceslas Lendamba, Kelly Mbombe Moghoa, Cyrille Bisseye
Summary: Urinary tract infections are a major cause of consultation worldwide, with E. coli and K. pneumoniae being the main uropathogenic strains. This study in Franceville found a high prevalence of UTIs caused by these bacteria, with high levels of antibiotic resistance, emphasizing the need for appropriate local antibiotic use.
INFECTION AND DRUG RESISTANCE
(2021)
Letter
Gastroenterology & Hepatology
Sohyun Lee, Nanjoo Park, Sujung Yun, Eunseon Hur, Jiwon Song, Hanna Lee, Yongsug Kim, Sangryeol Ryu
Summary: The study identified the presence of plasmid-mediated quinolone resistance (PMQR) genes in Salmonella enterica isolated from human salmonellosis patients in South Korea, with fluoroquinolone resistance mainly attributed to Salmonella 4,[5],12:i:- and Salmonella Typhimurium carrying the qnr gene.
Article
Medicine, General & Internal
August Wrotek, Julita Robakiewicz, Katarzyna Pawlik, Patryk Rudzinski, Izabela Pilarska, Aleksandra Jaron, Aleksandra Imielowska, Malgorzata Jarzebowska, Katarzyna Zablocka, Teresa Jackowska
Summary: Community-acquired pneumonia (CAP) has a significant impact on pediatric hospitalizations. The study analyzed the contribution of CAP to hospitalizations, its etiology in relation to age, and the role of inflammatory markers. It found that CAP accounted for a considerable proportion of hospitalizations and patient days, with viral pneumonia being more common than bacterial pneumonia. Serum inflammatory markers can assist in differentiating the causative factors.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Critical Care Medicine
Samah Meradji, Abouddihaj Barguigua, Mohamed Cherif Bentakouk, Kaotar Nayme, Khalid Zerouali, Dekhil Mazouz, Houria Chettibi, Mohammed Timinouni
Article
Infectious Diseases
Idrissa Diawara, Khalid Zerouali, Khalid Katfy, Abouddihaj Barguigua, Houria Belabbes, Mohammed Timinouni, Naima Elmdaghri
INFECTION GENETICS AND EVOLUTION
(2016)
Article
Infectious Diseases
Itto Maroui, Abouddihaj Barguigua, Asmae Aboulkacem, Khadija Ouarrak, Mohammed Sbiti, Housssain Louzi, Mohammed Timinouni, Abdelhaq Belhaj
JOURNAL OF INFECTION AND CHEMOTHERAPY
(2016)
Article
Infectious Diseases
Meradi Laarem, Abouddihaj Barguigua, Kaotar Nayme, Abdi Akila, Khalid Zerouali, Naima El Mdaghri, Mohammed Timinouni
JOURNAL OF INFECTION IN DEVELOPING COUNTRIES
(2017)
Article
Microbiology
Idrissa Diawara, Abouddihaj Barguigua, Khalid Katfy, Kaotar Nayme, Houria Belabbes, Mohammed Timinouni, Khalid Zerouali, Naima Elmdaghri
ANNALS OF CLINICAL MICROBIOLOGY AND ANTIMICROBIALS
(2017)
Article
Infectious Diseases
Btissam Arhoune, Bouchra Oumokhtar, Fouzia Hmami, Abouddihaj Barguigua, Mohammed Timinouni, Samira el Fakir, Fouzia Chami, Abdelhak Bouharrou
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2017)
Article
Environmental Sciences
Abdelwahid Assaidi, Mostafa Ellouali, Hassan Latrache, Mustapha Mabrouki, Mohammed Timinouni, Hafida Zahir, Safae Tankiouine, Abouddihaj Barguigua, El Mostafa Mliji
INTERNATIONAL JOURNAL OF ENVIRONMENTAL HEALTH RESEARCH
(2018)
Article
Engineering, Chemical
Abdelwahid Assaidi, Mostafa Ellouali, Hassan Latrache, Mustapha Mabrouki, Fatima Hamadi, Mohammed Timinouni, Hafida Zahir, Naima El Mdaghri, Abouddihaj Barguigua, El Mostafa Mliji
JOURNAL OF ADHESION SCIENCE AND TECHNOLOGY
(2018)
Article
Biodiversity Conservation
Abouddihaj Barguigua, Hamid Rguibi Idrissi, Kaoutar Nayme, Mohammed Timinouni
Article
Infectious Diseases
Fatima Zenati, Abouddihaj Barguigua, Kaotar Nayme, Fethi Benbelaid, Abdelmounaim Khadir, Chafika Bellahsene, Mourad Bendahou, Hassaine Hafida, Mohammed Timinouni
JOURNAL OF INFECTION IN DEVELOPING COUNTRIES
(2019)
Article
Agriculture, Dairy & Animal Science
K. Nayme, A. Barguigua, I. Diawara, F. El Otmani, N. Elmdaghri, K. Zerouali, M. Timinouni
BRITISH POULTRY SCIENCE
(2019)
Article
Infectious Diseases
Abdelwahid Assaidi, Mostafa Ellouali, Hassan Latrache, Mohammed Timinouni, Hafida Zahir, Abdelaziz Karoumi, Abouddihaj Barguigua, El Mostafa Mliji
MICROBIAL DRUG RESISTANCE
(2020)
Article
Infectious Diseases
N. El Hamzaoui, A. Barguigua, S. Larouz, M. Maouloua
NEW MICROBES AND NEW INFECTIONS
(2020)
Article
Agricultural Economics & Policy
Kaotar Nayme, Abouddihaj Barguigua, Brahim Bouchrif, Idrissa Diawara, Fatima El Otmani, Naima Elmdaghri, Khalid Zerouali, Mohammed Timinouni
BRITISH FOOD JOURNAL
(2017)
Letter
Urology & Nephrology
S. Natoubi, A. Barguigua, N. Zerhouni, N. Baghdad, M. Timinouni, A. Hilali, S. Amghar, K. Zerouali
AFRICAN JOURNAL OF UROLOGY
(2017)