Article
Biochemical Research Methods
Weidu Song, Chuanhao Zhang, Huichao Lin, Taiyi Zhang, Haixia Liu, Xiaowen Huang
Summary: The proposed real-time rotary PCR reaction system (RRP) meets the requirements of low cost, low-power consumption, potential for miniaturization, and portability. Experimental and theoretical results demonstrate its feasibility in the rapid quantitative detection of Pseudomonas aeruginosa and have broad application prospects in bacteria detection and food safety.
Article
Biochemical Research Methods
Shaolei Huang, Yiquan An, Bangchao Xi, Xianglian Gong, Zhongfu Chen, Shan Shao, Shengxiang Ge, Jun Zhang, Dongxu Zhang, Ningshao Xia
Summary: Nucleic acid detection is a method that directly identifies the presence of pathogenic microorganisms and has advantages of high sensitivity, commendable specificity, and a short window period. A real-time PCR system based on multiple temperature zones was proposed to solve the time-consuming problem, enabling fast detection speed and high sensitivity under extreme conditions.
Article
Ophthalmology
Satoko Nakano, Yasuhiro Tomaru, Toshiaki Kubota, Hiroshi Takase, Manabu Mochizuki, Norio Shimizu, Sunao Sugita
Summary: The Direct Strip PCR test offers rapid and accurate detection of infectious pathogens causing uveitis. It shows high concordance with qPCR results, even when used by beginners, indicating its reliability for clinical use. Additionally, it can be used for intraoperative diagnosis, improving the prognosis of infectious diseases.
Article
Food Science & Technology
Ya-wei Sun, Miao Qu, Jiayinaguli Tuersunbai, Xuan-yi Wang, Tuolihanayi Tuoliken, Xiao-hong Zhang, Gang Yao, Huijun Shi
Summary: This study compares the longissimus dorsi muscle of Xinjiang brown cattle and Angus cattle and explores the differences in muscle fiber types between the two. The results show that there are variations in mRNA expression levels and fiber types between the two breeds at different ages. Xinjiang brown cattle are more suitable for early fat calf production.
JOURNAL OF FOOD QUALITY
(2022)
Article
Parasitology
Gereon Schares, Majda Globokar Vrhovec, Mareen Tuschy, Maike Joeres, Andrea Baerwald, Bretislav Koudela, Jitender P. Dubey, Pavlo Maksimov, Franz J. Conraths
Summary: The 529-bp repeat of H. hammondi can be used to establish a quantitative real-time PCR assay, with excellent specificity and sensitivity. This assay is a valuable diagnostic tool for epidemiological and cell biological studies on H. hammondi and related parasites.
PARASITES & VECTORS
(2021)
Article
Optics
Damian Presti, Julian Bergier, Lucas Ripoll, Cristina Borio, Gustavo A. Torchia, Marcos Bilen
Summary: Since the global pandemic of SARS-CoV-2, the need for a low-cost and faster diagnostic tool for evaluating symptomatic patients has emerged. Point-of-care testing and point-of-need testing systems have been developed to provide accurate and rapid diagnostics. In this study, a bio-photonic device using an iso-thermal system was developed for detecting SARS-CoV-2. The device showed comparable sensitivity to the commercially used reference method and was built with simple and low-cost components, making it a high-efficiency and affordable instrument.
JOURNAL OF THE OPTICAL SOCIETY OF AMERICA A-OPTICS IMAGE SCIENCE AND VISION
(2023)
Article
Biochemical Research Methods
Renna L. Nouwairi, Larissa L. Cunha, Rachelle Turiello, Orion Scott, Jeff Hickey, Scott Thomson, Stuart Knowles, Jeff D. Chapman, James P. Landers
Summary: This article describes a field-forward prototype instrument capable of ultra-fast thermal cycling for real-time PCR amplification of DNA and RNA. The instrument can complete 40 cycles of real-time PCR in under 10 minutes, an 84% reduction in time compared to standard assays.
Article
Biochemical Research Methods
Byoung-Hoon Kang, Kyung-Won Jang, Eun-Sil Yu, Hyejeong Jeong, Ki-Hun Jeong
Summary: A multi-channel plasmonic real-time reverse-transcription polymerase chain reaction system is used to demonstrate high-throughput and multi-target detection of the SARS-CoV-2 virus with four simultaneous reactions in a single assay within 15 min.
Article
Agriculture, Dairy & Animal Science
X-J Li, L-Q Liu, H. Dong, J-J Yang, W-W Wang, Q. Zhang, C-L Wang, J. Zhou, H-Q Cheng
Summary: The study identified differentially methylated genes associated with fatty acid metabolism, muscle development, and pork quality through DNA methylation analysis of two pig breeds. Integrative analysis revealed a significant negative correlation between DNA methylation levels and gene expression levels. Differential methylation of genes related to fat metabolism, lipid metabolism, and skeletal muscle development may contribute to the growth and phenotypic differences observed among different pig breeds.
Article
Agriculture, Multidisciplinary
Li Cen-cen, Yu Shu-long, Ren Hai-feng, Wu Wei, Wang Ya-ling, Han Qiu, Xu Hai-xia, Xu Yong-jie, Zhang Peng-peng
Summary: The study collected data on long intergenic noncoding RNAs (lincRNAs) in porcine skeletal muscle development, identified differentially expressed lincRNAs during early pig embryonic development, predicted their potential target genes and functions, and laid the foundation for further research into lincRNAs functions.
JOURNAL OF INTEGRATIVE AGRICULTURE
(2021)
Article
Genetics & Heredity
Kaitlyn R. Daza, Deborah Velez-Irizarry, Sebastian Casiro, Juan P. Steibel, Nancy E. Raney, Ronald O. Bates, Catherine W. Ernst
Summary: This study integrated miRNA and gene expression data to identify 23 miRNA expression Quantitative Trait Loci (miR-eQTL) in pigs, with miR-874 being associated with multiple production traits. The results provide insight into genomic regions underlying miRNA expression variation and potential regulatory effects on economically important traits in the global pig industry.
FRONTIERS IN GENETICS
(2021)
Article
Chemistry, Analytical
Padideh Mohammadyousef, Miltiadis Paliouras, Mark A. Trifiro, Andrew G. Kirk
Summary: In response to the need for rapid infectious pathogen detection, researchers have been working on adapting PCR for POC applications. This paper introduces a compact plasmonic PCR thermocycler for fast DNA amplification using photothermal heating with gold nanorods and a VCSEL. An ultrasensitive real-time amplicon detection strategy is also reported, based on monitoring UV absorption of the PCR sample cycle-by-cycle. This system allows for high detection sensitivity and does not require specific probes or fluorophores.
Article
Genetics & Heredity
Ze Shu, Ligang Wang, Jinbu Wang, Longchao Zhang, Xinhua Hou, Hua Yan, Lixian Wang
Summary: In this study, high-throughput sequencing techniques were used to analyze the role of alternative splicing in the regulation of intramuscular fat deposition in pigs. The results revealed that alternative splicing isoforms could participate in skeletal muscle development and fatty acid metabolism, and identified genes and protein-protein interactions related to intramuscular fat. These findings provide valuable insights for further understanding the regulation of intramuscular fat deposition.
FRONTIERS IN GENETICS
(2022)
Article
Chemistry, Multidisciplinary
Shaw-Hwa Parng, Li-An Wu, Chien-Chih Kuo, Ping-Jung Wu, Yu-Yin Tsai, Chih-Hung Lee
Summary: This study proposes a method to generate droplets in a cylindrical tube for emulsion polymerase chain reaction. The validity of a non-planar droplet chip is verified by analyzing the light intensity of liquids in real-time during droplet processing. The study also compares two digital droplet PCR methods for measuring the copy numbers of EGFR.
Article
Agriculture, Multidisciplinary
Lin Yao, Hongmei Xin, Meng Qu, Yanhua Jiang, Yingying Guo, Fengling Li, Na Li, Zhijun Tan, Lianzhu Wang
Summary: The study developed a duplex real-time PCR method to specifically, sensitively, and accurately detect oilfish and escolar-derived components. The method showed high specificity without cross-reaction on other species, with a limit of detection for DNA of 0.0002 ng μL(-1) for R. pretiosus and 0.002 ng μL(-1) for L. flavobrunneum. Oilfish- and escolar-derived components were detected in 12 of the 70 commercial samples, consistent with DNA barcoding test results.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2021)
Article
Biochemistry & Molecular Biology
Michael Oster, Henry Reyer, Christian Gerlinger, Nares Trakooljul, Puntita Siengdee, Jonas Keiler, Siriluck Ponsuksili, Petra Wolf, Klaus Wimmers
Summary: Knowledge of gene expression profiles of PTGs contributes to understanding mineral homeostasis and PTH regulation. Long-term dietary intervention in pig offspring reveals molecular pathways controlling PTH levels in response to varying P supplies during fetal and postnatal life. The study highlights the importance of PTG mRNA profiles in reflecting physiological responses to different dietary P supplies.
Article
Agriculture, Dairy & Animal Science
N. A. Zinovieva, I. P. Sheiko, A. Dotsev, R. Sheiko, M. E. Mikhailova, A. A. Sermyagin, A. S. Abdelmanova, V. R. Kharzinova, H. Reyer, K. Wimmers, J. Solkner, N. Pleshanov, G. Brem
Summary: This study assessed the genetic diversity and population structure of Belarusian Red cattle, showing moderate genetic variability and clear differentiation from other European cattle breeds. The presence of ancestral genomic components from Danish Red and Brown Swiss breeds was visible, in line with the breed's history and recent development. Maintaining specific genomic components in Belarusian Red cattle is crucial for global genetic diversity, making them a valuable national genetic resource.
Article
Genetics & Heredity
Asghar Ali, Eduard Murani, Frieder Hadlich, Xuan Liu, Klaus Wimmers, Siriluck Ponsuksili
Summary: The study identified 13 miRNAs in the pig fetus's longissimus dorsi muscle significantly correlated to fetal weight, with their expression decreasing as fetal weight increased. Additionally, 1315 genes were found to be significantly correlated to fetal weight at 63 days post conception, with 135 genes identified as potential targets of the miRNAs mentioned above.Paths and biological processes important for fetal growth, development, and metabolism were enriched by these miRNAs and their target genes, suggesting their potential as biomarkers for prenatal fetal health and growth.
Article
Genetics & Heredity
Ibrahim Abousoliman, Henry Reyer, Michael Oster, Eduard Murani, Ismail Mohamed, Klaus Wimmers
Summary: Sheep play a critical role in Egypt's agricultural and livestock sectors. The Barki sheep breed, known for its adaptation to harsh environmental conditions, contributes significantly to meat production in semi-arid regions. A study using a genome-wide SNP panel identified genetic regions diversified between Egyptian Barki sheep with high and low growth performance traits, highlighting potential for improving growth traits in this breed.
Article
Agronomy
Dario Fornara, Elizabeth M. E. Ball, Christina Mulvenna, Henry Reyer, Michael Oster, Klaus Wimmers, Hanne Damgaard Poulsen, Arno Rosemarin
Summary: The over-supplementation of animal feeds with phosphorus leads to high rates of P excretion and negative environmental impacts. Adding phytase to pig and poultry diets can reduce P excretion, but the effects on plant-soil systems are poorly understood. This study found that phytase supplementation significantly influenced P content of animal waste and plant P dynamics, with intermediate phytase levels contributing to higher P use efficiency and lower soil P availability. The effects of dietary P content on P losses from soils varied depending on the type of organic amendment.
Article
Agriculture, Dairy & Animal Science
Christina C. Mulvenna, Ursula M. McCormack, Elizabeth Magowan, John McKillen, Mike R. Bedford, Carrie L. Walk, Michael Oster, Henry Reyer, Klaus Wimmers, Dario A. Fornara, M. Elizabeth E. Ball
Summary: Phytase has been a common addition to poultry diets for over 20 years, showing benefits in growth performance, nutrient digestibility, and bone parameters. This study found that superdosing phytase in rapeseed meal and maize dried distiller grains with solubles diets can improve performance, increase digestibility, and reduce P excretion by 15% compared to birds without phytase.
Article
Biochemistry & Molecular Biology
Maruf Hasan, Michael Oster, Henry Reyer, Siriluck Ponsuksili, Eduard Murani, Petra Wolf, Dagmar-Christiane Fischer, Klaus Wimmers
Summary: This study investigated the tissue-wide expression patterns of key genes related to vitamin D metabolism and FGF23 signaling in pigs fed different phosphorus diets. The results showed that non-renal tissues and cells have specific requirements for active vitamin D formation. Low phosphorus diet suppressed vitamin D signaling locally, while high phosphorus diet stimulated local vitamin D synthesis and regulation of FGF23 signaling in bone.
Article
Agriculture, Dairy & Animal Science
Adewunmi O. Omotoso, Henry Reyer, Michael Oster, Steffen Maak, Siriluck Ponsuksili, Klaus Wimmers
Summary: Phosphorus inclusion in broiler diets needs to be tailored to their physiological demands at different developmental stages. A timed nutritional conditioning strategy, such as P depletion, may enhance mineral homeostasis mechanisms and reduce dietary P supply. This study investigated the effects of dietary P depletion on broiler chickens at different developmental stages, revealing marked responses early on, but indications of compensatory mechanisms later. Endocrine control mediated by calcitriol, intestinal P uptake, and mineral mobilization from bones were identified as potential mechanisms for maintaining mineral homeostasis. Precise timing, duration, and extent of P depletion should be considered for optimized nutrient utilization in broiler husbandry.
Article
Chemistry, Analytical
Mohd. Rahil Hasan, Pradakshina Sharma, Shifa Shaikh, Saumitra Singh, Roberto Pilloton, Jagriti Narang
Summary: We have developed a paper-based experimental platform for analyzing dengue virus, which incorporates screen-printing technology and black carbon conductive ink. The platform utilizes both two-electrode and three-electrode systems, with an LOD of 0.1 μg/mL for detecting the virus. The three-electrode system exhibits a significantly higher current range and amplification compared to the two-electrode system, making it a viable option for sensitive detection of dengue virus and other diseases.
Article
Nutrition & Dietetics
Maruf Hasan, Michael Oster, Henry Reyer, Klaus Wimmers, Dagmar-Christiane Fischer
Summary: Vitamin D-3 (Vit D-3) and 25(OH)D-3 are used as dietary sources of active vitamin D in pig husbandry, and while their primary effects are on the intestine, kidney, and bone, they also have wide-ranging effects on peripheral tissues. The use of Vit D-3 and 25(OH)D-3 in pig nutrition has been shown to have different outcomes in terms of reproductive capacities, growth performance, immunity, and bone development.
BRITISH JOURNAL OF NUTRITION
(2023)
Correction
Nutrition & Dietetics
Maruf Hasan, Michael Oster, Henry Reyer, Klaus Wimmers, Dagmar-Christiane Fischer
BRITISH JOURNAL OF NUTRITION
(2023)
Article
Agriculture, Dairy & Animal Science
Hanne Honerlagen, Henry Reyer, Ibrahim Abou-Soliman, Dierck Segelke, Siriluck Ponsuksili, Nares Trakooljul, Norbert Reinsch, Bjoern Kuhla, Klaus Wimmers
Summary: Improving the nitrogen-utilization efficiency (NUE) of dairy cows through breeding selection has benefits in terms of nutrition, environment, and economics. The individual milk urea concentration (MU) of cows has been proposed as an indicator trait for NUE. This study aimed to identify different microbial genera in the rumen of Holstein cows with divergent genomic breeding values for MU (GBVMU). The identified microbial genera were further investigated for their correlations with MU and other NUE-associated traits, providing insight into genetically determined N-utilization in Holstein cows.
JOURNAL OF DAIRY SCIENCE
(2023)
Article
Cell Biology
Julia Brenmoehl, Elli Brosig, Nares Trakooljul, Christina Walz, Daniela Ohde, Antonia Noce, Michael Walz, Martina Langhammer, Stefan Petkov, Monika Roentgen, Steffen Maak, Christina E. E. Galuska, Beate Fuchs, Bjoern Kuhla, Siriluck Ponsuksili, Klaus Wimmers, Andreas Hoeflich
Summary: This study investigated the contribution of non-mitochondrial and mitochondrial metabolic pathways in contracting muscles during repeated training. The results showed that marathon mice displayed increased glucose and fatty acid oxidation, reduced lactate dehydrogenase activity, and inhibited lactate formation compared to trained control mice. This suggests that marathon mice have enhanced metabolic flexibility towards lipid catabolism regulated by lactate dehydrogenase.
Review
Agriculture, Dairy & Animal Science
Benjamin Diehl, Michael Oster, Andreas Vernunft, Klaus Wimmers, Hartwig Bostedt
Summary: The losses of piglets in commercial pig farming remain a concern and sustainable breeding and management strategies are needed. This review systematically presents the three phases of neonatal adaptation in piglets and highlights the key developments and influencing factors in each phase.
ARCHIVES ANIMAL BREEDING
(2022)
Review
Biochemistry & Molecular Biology
Fabio Sarais, Alvaro Perdomo-Sabogal, Klaus Wimmers, Siriluck Ponsuksili
Summary: This review focuses on the latest discoveries of tsRNAs, with special attention to molecules initially thought to be associated with tiRNAs. The paper outlines their biological functions, provides a collection of useful databases, and discusses future research perspectives and applications in livestock basic and applied research.