Article
Engineering, Biomedical
Mathaeus Tschaikowsky, Tanja Neumann, Sofia Brander, Heiko Haschke, Bernd Rolauffs, Bizan N. Balzer, Thorsten Hugel
Summary: Understanding the function and dysfunction of hierarchically organized biomaterials or tissues requires studying different length scales. Atomic force microscopy (AFM) combined with fluorescence microscopy can provide detailed information from millimeter to nanometer scales. This approach is not only applicable to cartilage but also useful for characterizing native biological tissues from macro- to nanoscale.
ACTA BIOMATERIALIA
(2021)
Article
Endocrinology & Metabolism
Rasmus Klose-Jensen, Louise Brandt Hartlev, Jesper Skovhus Thomsen, Jens Randel Nyengaard, Lene Warner Thorup Boel, Mogens Laursen, Trine Bay Laurberg, Kristian Stengaard-Pedersen, Ellen-Margrethe Hauge
Summary: The volume and surface area of calcified cartilage in the femoral head were significantly larger in patients with osteoarthritis (OA) compared to healthy subjects. The volume of calcified cartilage was positively correlated with the volume of the femoral head in OA patients, indicating a potential association with endochondral ossification in the pathogenesis of OA.
Article
Anatomy & Morphology
Emily J. Hargrave-Thomas, Ashvin Thambyah
Summary: The study examines structural changes in the osteochondral junction tissues in mature bovine patellae showing different cartilage health grades, finding that bone spicules are associated with all grades of cartilage tissue studied, with the most occurring in the intermediate stages of tissue health. This suggests that bone formation in osteoarthritis is more akin to a combination of primary bone remodelling and de novo bone formation.
JOURNAL OF ANATOMY
(2021)
Review
Anatomy & Morphology
Lucinda A. E. Evans, Andrew A. Pitsillides
Summary: Articular calcified cartilage (ACC) has been dismissed by some as a remnant of endochondral ossification, but recent research suggests that it may play a role in knee joint osteoarthritis (OA). ACC is less studied compared to other joint tissues, but its anatomical and metabolic features may be important and deserve further investigation.
JOURNAL OF ANATOMY
(2022)
Review
Immunology
James Orman Early, Lauren E. Fagan, Annie M. Curtis, Oran D. Kennedy
Summary: Inflammation is an important biological response to tissue damage, but dysregulation of the process can lead to tissue damage. Mitochondria play a key role in orchestrating the inflammatory response, beyond their traditional function in energy production. Osteoarthritis, previously thought of as non-inflammatory, now appears to involve chronic inflammation as well.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Biotechnology & Applied Microbiology
Yanxi Liu, Karan M. Shah, Jian Luo
Summary: Articular cartilage is avascular and has limited ability to repair itself, leading to degenerative joint diseases. Current clinical treatments often result in mechanically inferior cartilage. Understanding factors affecting cartilage homeostasis and exploring emerging regenerative approaches are crucial for future treatment options.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Acoustics
Theresa H. Lye, Omar Gachouch, Lisa Renner, Sefer Elezkurtaj, Hannes Cash, Daniel Messroghli, Kay Raum, Jonathan Mamou
Summary: This study assessed the performance of a high-frequency linear array transducer in detecting early osteoarthritis. The results showed that the transducer has comparable screening capabilities for early osteoarthritis to research-grade single-element ultrasound and MRI.
ULTRASOUND IN MEDICINE AND BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Ke Li, Xing Ji, Rebecca Seeley, Wen-Chih Lee, Yu Shi, Fangfang Song, Xueyang Liao, Chao Song, Xiaobin Huang, Danielle Rux, Ju Cao, Xiaoji Luo, Steven M. Anderson, Wei Huang, Fanxin Long
Summary: This study found that the expression of glucose transporter Glut1 is reduced in articular cartilage in osteoarthritis (OA). Inducible deletion or forced expression of Glut1 can accelerate or protect against cartilage destruction, respectively. Moreover, in diabetic mice, both Glut1 expression and glycolysis rate are diminished in articular cartilage, resulting in more severe cartilage destruction.
Article
Engineering, Biomedical
Mathaeus Tschaikowsky, Sofia Brander, Vanessa Barth, Ralf Thomann, Bernd Rolauffs, Bizan N. Balzer, Thorsten Hugel
Summary: This study reveals changes in the thickness and composition of collagen fibers at the onset of osteoarthritis. Fibrocartilage-like tissue rich in type I collagen is formed in intact cartilage during the early stages of the disease, while thick fibers are completely absent in advanced osteoarthritis. The findings provide insights into the understanding of other native biological tissues and the development of sustainable biomaterials.
ACTA BIOMATERIALIA
(2022)
Article
Immunology
Fei Cao, Yang Chen, Xing Wang, Li-Min Wu, Mei Tian, Han-Yu Li, Hai-Bo Si, Bin Shen
Summary: MicroRNAs have received significant attention in recent years for their potential role in osteoarthritis pathogenesis. A study on rats showed that intra-articular injections of miR-140-5p can effectively alleviate early-stage OA progression by modulating various biological processes and pathways. In particular, multiple injections of miR-140-5p demonstrated better therapeutic effects compared to single injections in alleviating early-stage OA.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Phaedra Winstanley-Zarach, Gregor Rot, Shweta Kuba, Aibek Smagul, Mandy J. J. Peffers, Simon R. R. Tew
Summary: Polyadenylation (polyA) defines the 3' boundary of a transcript's genetic information. Alternative polyadenylation (APA) transcripts can exist for a gene, causing variance in regulatory domains and potential coding sequence changes. This study analyzed the polyA site distribution in healthy and osteoarthritic knee articular chondrocytes and found modest differential use of polyadenylation sites between the two conditions, suggesting potential functional relevance in affected genes. This characterization of polyadenylation landscape contributes to understanding the role of polyA in knee osteoarthritis, distinguishing it from other pathologies such as cancer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Immunology
Taku Saito
Summary: The superficial zone of articular cartilage produces PRG4, which contributes to smooth joint motion. PRG4, besides being an extracellular matrix protein, has novel signaling effects. Various molecules and signaling pathways regulate the expression of PRG4 and the superficial zone. Further research on PRG4-related pathways and cells may uncover the mechanisms underlying joint homeostasis.
INFLAMMATION AND REGENERATION
(2022)
Review
Biotechnology & Applied Microbiology
Weiyang Wang, Ruixi Ye, Wenqing Xie, Yueyao Zhang, Senbo An, Yusheng Li, Yang Zhou
Summary: The calcified cartilage layer (CCL) plays important roles in joint development and activities, with abnormal alterations potentially indicating osteoarthritis. Tissue engineering reconstruction of CCL in damaged cartilage is of significant importance.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Orthopedics
M. Jarraya, A. Guermazi, J. W. Liew, I. Tolstykh, J. A. Lynch, P. Aliabadi, D. T. Felson, M. Clancy, M. Nevitt, C. E. Lewis, J. Torner, T. Neogi
Summary: The aim of this study was to report the prevalence of CT-detected intra-articular mineralization. It was found that 9.8% of knees had intra-articular mineralization based on CT imaging. Mineralization predominantly occurred in the three compartments of the joint, especially the patellofemoral compartment.
OSTEOARTHRITIS AND CARTILAGE
(2023)
Article
Biotechnology & Applied Microbiology
Tianyu Gao, Alexander J. Boys, Crystal Zhao, Kiara Chan, Lara A. Estroff, Lawrence J. Bonassar
Summary: This study utilized confocal Raman microspectroscopy to investigate the degradation of articular cartilage, specifically focusing on spatially mapping the loss of glycosaminoglycans (GAGs), providing a technique with high spatial resolution to measure cartilage degradation without tissue sections.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Orthopedics
Taryn Saggese, Ashvin Thambyah, Kelly Wade, Susan Read McGlashan
Article
Clinical Neurology
Nurul Haiza Sapiee, Ashvin Thambyah, Peter A. Robertson, Neil D. Broom
Article
Engineering, Biomedical
Shuvashis Das Gupta, Mikko A. J. Finnila, Sakari S. Karhula, Sami Kauppinen, Antti Joukainen, Heikki Kroger, Rami K. Korhonen, Ashvin Thambyah, Lassi Rieppo, Simo Saarakkala
ACTA BIOMATERIALIA
(2020)
Article
Biophysics
Isabela A. Monteiro, Tarek Kollmetz, David S. Musson, Sue R. McGlashan, Jenny Malmstrom
Article
Engineering, Biomedical
Emma Te Tumanako Brown, Alicia Helena Antonetta Damen, Ashvin Thambyah
CLINICAL BIOMECHANICS
(2020)
Article
Orthopedics
Mark F. Zhu, Brittany Smith, Sanjeev Krishna, David S. Musson, Peter R. Riordan, Sue R. McGlashan, Jillian Cornish, Jacob T. Munro
BMC MUSCULOSKELETAL DISORDERS
(2020)
Article
Engineering, Biomedical
J. Workman, S. McGlashan, A. Thambyah
JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS
(2020)
Review
Orthopedics
Mei Lin Tay, Sue R. McGlashan, A. Paul Monk, Simon W. Young
Summary: This study found that the most common failure modes for medial UKAs were aseptic loosening and OA progression. Early failures were often caused by infection, bearing dislocation, and fracture; mid-term failures were mainly due to OA progression, aseptic loosening, and pain; while late-term failures were primarily due to OA progression. Rates of failure varied depending on implant design and cement use, highlighting the importance of careful consideration of these factors in UKA procedures.
ARCHIVES OF ORTHOPAEDIC AND TRAUMA SURGERY
(2022)
Article
Biophysics
Sophia Leung, Jung-Joo Kim, David S. Musson, Sue R. McGlashan, Jillian Cornish, Iain Anderson, Vickie B. K. Shim
Summary: This study describes the design and validation of a novel system for analyzing chondrocyte deformation patterns under mechanical stimulation. The computer-controlled device accurately applies compressive, tensile, and shear strains to hydrogel constructs and is highly reproducible. It characterized the strains within constructs and validated with various methods, providing insights into chondrocyte response to complex physiologically relevant deformation profiles.
JOURNAL OF BIOMECHANICAL ENGINEERING-TRANSACTIONS OF THE ASME
(2021)
Article
Orthopedics
Joanna L. James, Anandita Umapathy, Sonia Srinivasan, Claire N. Barker, Anna Brooks, James Hearn, Ashika Chhana, Eloise Williams, Hilary Sheppard, Sue R. McGlashan
Summary: The study found differences in chondrogenesis potential between first-trimester and term placental MSCs, with term MSCs showing significantly higher GAG content compared to BM-MSCs. Only first-trimester MSCs demonstrated significant changes in chondrogenic gene expression during induction compared to undifferentiated MSCs.
Letter
Rheumatology
Nicola Dalbeth, Mariam Alhilali, Peter Riordan, Ravi Narang, Ashika Chhana, Sue McGlashan, Anthony Doyle, Mariano Andres
ARTHRITIS & RHEUMATOLOGY
(2022)
Article
Pharmacology & Pharmacy
Hani Abdeltawab, Scott M. Bolam, Jagdish K. Jaiswal, Sue R. McGlashan, Simon W. Young, Andrew Hill, Darren Svirskis, Manisha Sharma
Summary: A thermoresponsive in situ gelling system based on poloxamer was developed for sustained delivery of bupivacaine hydrochloride and ketorolac tromethamine. The system demonstrated prolonged drug release and potential clinical benefits.
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
(2022)
Article
Biotechnology & Applied Microbiology
Subhajit Konar, Scott M. Bolam, Brendan Coleman, Nicola Dalbeth, Sue R. McGlashan, Sophia Leung, Jillian Cornish, Dorit Naot, David S. Musson
Summary: Tendinopathy is characterized by pathological changes in tendon matrix composition, architecture, and stiffness, with inflammation also playing an important role. This study found that substrate stiffness affects tendon-derived cells and macrophages. Tendon-derived cells showed minor responses to substrate stiffness, while macrophages exhibited a more inflammatory phenotype on non-physiological stiffness substrates. These subtle variations in matrix stiffness may contribute to the onset and progression of tendinopathy.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Proceedings Paper
Optics
Matthew Goodwin, Ashvin Thambyah, Frederique Vanholsbeeck
OPTICAL COHERENCE IMAGING TECHNIQUES AND IMAGING IN SCATTERING MEDIA III
(2019)