Article
Cell Biology
Letizia Penolazzi, Maria Pina Notarangelo, Elisabetta Lambertini, Valentina Vultaggio-Poma, Mario Tarantini, Francesco Di Virgilio, Roberta Piva
Summary: The main objective of this study was to determine the presence of the P2X7 receptor in human skeletal system cells. P2X7 receptor was found to be localized not only in the plasma membrane and cytoplasm, but also in the nucleus and mitochondria of osteoblasts, chondrocytes, and intervertebral disc cells.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Ioanna Tremi, Sophia Havaki, Sofia Georgitsopoulou, Nefeli Lagopati, Vasilios Georgakilas, Vassilis G. Gorgoulis, Alexandros G. Georgakilas
Summary: The article discusses the combined effects of ionizing radiation with high-Z metallic nanoparticles, particularly gold, on DNA damage. It highlights the use of transmission electron microscopy (TEM) to study these effects at a detailed cellular level, focusing on the uptake and damage of nanoparticles in cells. The goal is to better understand the radiosensitization mechanisms of nanoparticles.
Article
Biochemistry & Molecular Biology
Lidia Gaffke, Karolina Pierzynowska, Estera Rintz, Zuzanna Cyske, Izabela Giecewicz, Grzegorz Wegrzyn
Summary: Mucopolysaccharidoses (MPS) are a group of inherited metabolic diseases characterized by the accumulation of incompletely degraded glycosaminoglycans in lysosomes. Changes in cellular organelles were observed in MPS fibroblasts, including dysregulation of gene expression and alterations in organelle morphology. Normalization of GAG levels corrected morphologies of some organelles but failed to improve gene expression regulation, suggesting limitations of enzyme replacement therapy in treating MPS symptoms.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Plant Sciences
T. C. Lee, K. H. Lin, M. Y. Huang, C-M Yang
Summary: By using immunogold-labelling technique, the study found that Chlorophyllases are mainly located in parts of the chloroplast, cytosol, and vacuoles in different developmental stages of Pm leaves. At the senescent stage, Chlorophyllases were mainly detected in vacuoles in chloroplasts.
BIOLOGIA PLANTARUM
(2021)
Article
Cell Biology
Andrew S. Belmont
Summary: This work reviews the progress in studying nuclear compartments, including nuclear structures, bodies, and chromosome domains. By comparing different research methods, particularly microscopy and genomics, we explore the evolving concept of nuclear compartments. Additionally, this review discusses the establishment and maintenance mechanisms of nuclear compartments, as well as their potential functions, such as the role of nuclear speckles in gene expression regulation.
COLD SPRING HARBOR PERSPECTIVES IN BIOLOGY
(2022)
Article
Neurosciences
Amina Aboufares El Alaoui, Molly Jackson, Mara Fabri, Luisa de Vivo, Michele Bellesi
Summary: This study utilized serial block-face scanning electron microscopy to reconstruct and describe the intracellular organelles in perisynaptic astrocytic processes (PAPs) in two different mouse cortical regions. Five distinct organelles were identified within the PAPs, distributed among three categories. The majority of PAPs belonged to the leaflets category, with branches and branchlets displaying different organelle distributions. Mitochondria and ER cisternae were the most common organelles observed, with endosomes and phagosomes also frequently associated with the same PAP.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Gines Avila-Perez, Maria Teresa Rejas, Francisco Javier Chichon, Milagros Guerra, Jose Jesus Fernandez, Dolores Rodriguez
Summary: This study used electron tomography to characterize the replication organelles of toroviruses, revealing the formation of a reticulovesicular network in BEV-infected cells. The outer membranes of DMVs were found to be interconnected with each other and with the ER, with paired ER membranes likely representing early structures that evolve into DMVs. Late-stage paired membranes forming small spherule-like invaginations were also observed, showing similarities but also differences with true spherules described for coronaviruses.
MOLECULAR MICROBIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Martin Sachse, Isabel Fernandez de Castro, Raquel Tenorio, Cristina Risco
Summary: Transmission electron microscopy (TEM) is essential for studying virus-cell interactions. Immunogold and metal-tagging transmission electron microscopy (METTEM) are two widely used labeling methods that contribute to the study of viral infections. Immunogold utilizes antibodies and electron dense gold particles while METTEM uses metallothionein (MT) as a clonable tag. Together, these methods provide a powerful tool for investigating virus-cell interactions.
MOLECULAR MICROBIOLOGY
(2023)
Article
Neurosciences
Jung-Hwa Tao-Cheng, Virginia Crocker, Sandra Lara Moreira, Rita Azzam
Summary: Immunogold labeling is a useful technique for protein localization and quantification at the electron microscopy level. Methodological issues and experiences from studies on protein distribution in cell cultures and brain tissues were summarized. Careful determination of fixation conditions, tissue processing, and evaluation of labeling credibility are crucial for optimal results.
Article
Plant Sciences
Qiang Sun
Summary: This study investigated the structure and polysaccharide composition of intervessel pit membranes in grapevine using an immunogold-scanning electron microscopy technique. The results showed that intervessel pit membranes displayed diverse structural variations and contained different major groups of pectic and hemicellulosic polysaccharides with distinct spatial distributions and abundance. This information is crucial to understand the roles of intervessel pit membranes in water transport regulation and plant susceptibility to vascular diseases.
Review
Cell Biology
Patrick C. Nahirney, Marie-Eve Tremblay
Summary: Understanding the fine structure of the brain is crucial for understanding its normal and abnormal functioning. High-resolution electron microscopic techniques provide insights into different cell types in the brain, but identifying them remains a challenge. Ultrastructural analyses can reveal changes in brain function during aging and disease, and immunolabeling techniques can help visualize subtle differences in cells across health and disease contexts.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Maryna Ivanchenko, Artur A. Indzhykulian, David P. Corey
Summary: Hair cells in the vertebrate inner ear have actin-filled protrusions called stereocilia which mediate mechanosensitivity. Hereditary deafness is often linked to abnormalities in stereocilia bundles, making them essential for understanding genetic hearing loss models. Electron microscopy techniques, such as TEM and SEM, are crucial for studying stereocilia morphology and protein localization.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Urology & Nephrology
Sebastien Eymieux, Elodie Miquelestorena-Standley, Nolwenn Rabot, Valentin Maisons, Guy Touchard, Emmanuelle Blanchard
Summary: A rare case of crystalline podocytopathy was reported, highlighting the key role of electron microscopy and immunogold in determining the location and composition of crystals.
CLINICAL KIDNEY JOURNAL
(2022)
Review
Chemistry, Analytical
Hyo Won Lee, Vinayak Juvekar, Dong Joon Lee, Hwan Myung Kim
Summary: Biological events occur in specific cellular organelles to perform specialized roles in cells and collaborate with other organelles. Various methods have been used to study organelle dynamics, including two photon microscopy. This review focuses on the design strategies, photo-physical properties, and bioimaging applications of recent two-photon probes specifically localized in the endoplasmic reticulum, Golgi, lipid droplets, lysosomes, plasma membrane, and mitochondria. The summary is limited to research from 2017 onwards. © 2023 Elsevier B.V. All rights reserved.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Shweta Suiwal, Mayur Dembla, Karin Schwarz, Rashmi Katiyar, Martin Jung, Yvonne Carius, Stephan Maxeiner, Marcel A. Lauterbach, C. Roy D. Lancaster, Frank Schmitz
Summary: Unc119 protein mediates transport of myristoylated proteins to the photoreceptor outer segment and is also enriched in photoreceptor synapses. The synaptic ribbon could be involved in the discharge of Unc119-bound lipid-modified proteins. Proteins associated with Unc119 may play important roles in photoreceptor synapses.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)