期刊
JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY
卷 23, 期 8, 页码 1121-1124出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/b802338g
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To investigate the toxicological effects of nanomaterials, experimental studies on the absorption and accumulation in organisms are of broad interest. In the present study, Caenorhabditis elegans (C. elegans) was used as a model'' organism to investigate the bioaccumulation and toxicological effects of engineered copper nanoparticles with a scanning technique of microbeam synchrotron radiation X-ray fluorescence (m-SRXRF). The adult hermaphrodite is anatomically simple with 959 somatic cells and 1 mm in length. The mapping results of the whole organism indicate that the exposure to copper nanoparticles can result in an obvious elevation of Cu and K levels, and a change of bio-distribution of Cu in nematodes. Accumulation of Cu occurs in the head and at a location 1/3 of the way up the body from the tail compared to the un- exposed control. In contrast, a higher amount of Cu was detected in other portion of worm body, especially in its excretory cells and intestine when exposed to Cu(2+). The results compared well with total Cu levels in nematodes, which were 4.10 +/- 0.54, 12.32 +/- 0.49 and 5.22 +/- 0.63 mu g g(-1) dry weight for the PBS, Cu(2+) and Cu nanoparticle groups, respectively, measured by ICP- MS. The nondestructive and multi- elemental m- SRXRF provides an important tool for mapping the elemental distribution in the whole body of a single tiny nematode at lower levels.
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