期刊
JOURNAL OF ANALYTICAL AND APPLIED PYROLYSIS
卷 90, 期 1, 页码 13-17出版社
ELSEVIER
DOI: 10.1016/j.jaap.2010.09.010
关键词
Pyrolysis-gas chromatography; Fingerprint; Dendrobium candidum Wall. ex Lindl.; Dendrobium; Hierarchical cluster analysis
资金
- Natural Science Foundation of Zhejiang Province [Y307602]
The pyrogram fingerprints of Dendrobium candidum Wall. ex Lindl. samples from 3 different growing places and 2 other different species were analyzed on the basis of pyrolysis-gas chromatography. An amount of 0.4 mg of sample powder was pyrolyzed in a vertical microfurnace pyrolyzer at 450 degrees C, and the products were directly introduced into a gas chromatograph equipped with a flame ionization detector or a mass spectrometer. Then, each sample was characterized by the relative peak area of 40 peaks in thus obtained pyrogram. The pyrogram fingerprints of 16 samples from different growing places and species showed good similarity and reproducibility with the relative standard deviations (RSDs) of the retention time less than 0.12% (n = 5) and the RSDs of the relative percent of peak areas less than 3.77% (n = 5). Furthermore, the discrimination of the samples from different growing places and species was achieved by hierarchical cluster analysis via recognizing the 16 x 40 data matrix. Thus, the results proved the Py-GC fingerprint combined with chemometric approach is a simple, rapid and selective method which is suitable for the quality control of the raw materials of herbal medicine. (C) 2010 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据