Article
Chemistry, Multidisciplinary
Paulo H. Jr Jr Olivieri, Marcelo B. Jesus, Helena B. Nader, Giselle Z. Justo, Alioscka A. Sousa
Summary: This study reveals that surface glycosaminoglycans (GAGs) can regulate the cellular internalization of nanoparticles. Anionic nanoparticles are hindered by the charged surface GAGs, while cationic nanoparticles interact favorably with GAGs. Further investigation shows that the interaction of nanoparticles with GAGs is different, and heparan sulfate plays a crucial role in the cellular internalization of cationic nanoparticles.
Article
Chemistry, Multidisciplinary
Marianna Barbalinardo, Jessika Bertacchini, Linda Bergamini, Maria Sara Magaro, Luca Ortolani, Alessandra Sanson, Carla Palumbo, Massimiliano Cavallini, Denis Gentili
Summary: The surface properties of nanoparticles can be effectively regulated by controlling the surface ligand density, which in turn affects serum protein adsorption, cellular uptake, and cytotoxicity. Tuning the surface properties allows for the control of biological outcomes by regulating the ability of nanoparticles to adsorb serum proteins.
Article
Pharmacology & Pharmacy
Italo Moglia, Margarita Santiago, Simon Guerrero, Monica Soler, Alvaro Olivera-Nappa, Marcelo J. Kogan
Summary: The study found that ferritin-encapsulated gold nanoparticles have low toxicity on human cell lines, and efficient cellular ferritin uptake depends on the specific H or L protein chains forming the ferritin protein cage and the presence of metallic cargo.
Article
Chemistry, Physical
Hui-Chen Chen, Mei-Lang Kung, Wei-Xiang Huang, Ru-Huei Fu, Alex Yang-Hao Yu, Ya-Tsu Yang, Huey-Shan Hung
Summary: The study utilized a novel AuNP-Col nanocarrier to deliver SDF-1 alpha, enhancing the biological performance, cellular uptake and proliferation capacity of DCs vaccine. It also promoted DCs migration and induction of T cell cytokine production.
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
(2021)
Review
Chemistry, Multidisciplinary
Patrycja Talarska, Maciej Boruczkowski, Jakub Zurawski
Summary: Silver and gold nanoparticles are widely used in various household products, raising concerns about their potential impact. Research is focusing on the effects of nanoparticles on cell function and environmentally friendly alternatives to chemically synthesized nanoparticles.
Article
Biophysics
Bingqing Lu, A. Jan Hendriks, Tom M. Nolte
Summary: A generic model was developed to predict the cellular uptake of nanoparticles (NPs) with organic coatings, taking into account physicochemical interactions. The model accurately estimated the uptake of neutrally charged unhalogenated NPs in six different cell types, providing a reference for ecological and human risk assessment of NPs or microparticles.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2022)
Article
Chemistry, Multidisciplinary
Bryan E. White, Molly K. White, Zeid A. Nima Alsudani, Fumiya Watanabe, Alexandru S. Biris, Nawab Ali
Summary: This study investigates the uptake mechanism and localization of gold nanorods in SKBR-3 and MCF-7 breast cancer cell lines. The results demonstrate differences and similarities in the interaction between nanorods and cells of the two cell lines. The cellular uptake of gold nanorods in SKBR-3 and MCF-7 was accurately quantified using ICP-MS, and the intracellular nanoparticle localization was qualitatively tracked using transmission electron microscopy.
Article
Toxicology
Maria Enea, Eulalia Pereira, Joana Costa, Maria Elisa Soares, Diana Dias da Silva, Maria de Lourdes Bastos, Helena Ferreira Carmo
Summary: The study compared the cytotoxic effects of gold nanoparticles with different size, shape, and capping on liver cells, finding that smaller 15 nm spheres displayed the highest toxicity. Toxicological effects were influenced by capping, size, and cell type, with citrate-capped nanoparticles more toxic than MUA, 15 nm nanoparticles more toxic than 60 nm ones, and primary rat hepatocytes more sensitive than HepaRG cells. The presence of FBS significantly influenced toxicity outcomes, with higher internalization of AuNPs in PRH compared to HepaRG cells.
TOXICOLOGY IN VITRO
(2021)
Article
Chemistry, Multidisciplinary
Sara Michelini, Francesco Barbero, Alessandra Prinelli, Philip Steiner, Richard Weiss, Thomas Verwanger, Ancuela Andosch, Ursula Lutz-Meindl, Victor F. Puntes, Damjana Drobne, Albert Duschl, Jutta Horejs-Hoeck
Summary: The study revealed that gold nanoparticles (AuNPs) may impact dendritic cell function and alter immune responses, especially in the presence of microbial inflammatory stimulus lipopolysaccharide (LPS). The findings suggest that AuNPs are safe in homeostatic conditions but caution is needed in patients with infections or immune system disorders.
Article
Chemistry, Multidisciplinary
Viktoriya Sokolova, Jana-Fabienne Ebel, Sebastian Kollenda, Kai Klein, Benedikt Kruse, Claudia Veltkamp, Christian M. Lange, Astrid M. Westendorf, Matthias Epple
Summary: Ultrasmall gold nanoparticles are capable of penetrating intestinal cells, colorectal cancer cells, and related 3D structures, while dissolved dyes cannot be taken up by these cells or structures. The cellular uptake of ultrasmall gold nanoparticles is found to occur through different endocytosis pathways. The nanoparticles conjugated with the cytostatic drug doxorubicin exhibit enhanced cytotoxicity against tumor spheroids.
Article
Biochemical Research Methods
Mona Mihailescu, Luminita C. Miclea, Ana M. Pleava, Nicolae Tarba, Eugen N. Scarlat, Raluca D. Negoita, Mihaela G. Moisescu, Tudor Savopol
Summary: We propose a method that combines imaging tools and computational tools to locate and detect unlabeled nanoparticles (NPs) in cells and evaluate their internal traffic. The method utilizes a dark field CytoViva optical system and combines 3D reconstructions of double fluorescently labeled cells with hyperspectral images. MATLAB scripts are used to process the images and localize NPs in different cell regions. The method provides important parameters to assess uptake efficiency and shows consistent results with biochemical analyses.
BIOMEDICAL OPTICS EXPRESS
(2023)
Article
Biochemistry & Molecular Biology
Leila Baghani, Niloofar Noroozi Heris, Fatemeh Khonsari, Sajad Dinarvand, Meshkat Dinarvand, Fatemeh Atyabi
Summary: This study suggests that the simply synthesized AuNPs-TMC are novel, effective, and promising nanocarriers for siRNA delivery, and AuNPs-TMC/EGFR-siRNA appears to be a potential therapeutic agent for breast cancer treatment.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Review
Pharmacology & Pharmacy
Preeti Sharma, Alakesh Alakesh, Siddharth Jhunjhunwala
Summary: Particle-based systems are widely used in the clinic, but internalization of particles may have unintended effects on immune cell functions. Understanding the impact of particles on immune cells is crucial for designing biocompatible and efficient particle formulations.
TRENDS IN PHARMACOLOGICAL SCIENCES
(2022)
Article
Pharmacology & Pharmacy
Suvadeep Mal, Tiyasa Saha, Asim Halder, Sudhir Kumar Paidesetty, Suvadra Das, Wong Tin Wui, Urmi Chatterji, Partha Roy
Summary: The World Health Organization has identified breast cancer as the most common form of cancer in recent times. Triple negative breast cancer, the deadliest sub-type, is characterized by a high rate of metastasis and poor prognosis due to the lack of progesterone, estrogen, and HER-2 receptors. This study explores the use of flavonoid-conjugated gold nanoparticles as targeted therapeutics against this subtype.
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2023)
Article
Neurosciences
Yunliang Zang, Erik De Schutter
Summary: This study explored the coding strategy used by individual neurons in the cerebellar Purkinje cells, finding that increasing input intensity shifts cells from linear rate-coders to burst-pause timing-coders through triggering dendritic spikes. Both linear and burst-pause computations use individual branches as computational units, challenging the traditional view of these cells as linear point neurons. Dendritic spike thresholds can be regulated by various factors to expand the dynamic range of information processing.
JOURNAL OF NEUROSCIENCE
(2021)
Article
Oncology
E. Le Reun, A. Casutt, A. Durham, H. Bouchaabe, E. Romano, A. Lovis, T. Krueger, C. Von Garniercg, E. M. Oezsahin, R. Kinj
Summary: This study compared three different radiotherapy devices for pulmonary stereotactic radiotherapy: CyberKnife, Helical Tomotherapy, and volumetric modulated arc therapy (VMAT). The results showed that CyberKnife treatments had comparable tumor volume, smaller planning treatment volume, and lower V5 values compared to other techniques. The incidence of toxicity was lower when V5 values were reduced. CyberKnife is particularly attractive for multiple courses of lung SBRT or lung reirradiation. Rating: 8/10
CANCER RADIOTHERAPIE
(2023)
Article
Materials Science, Multidisciplinary
Kevin Oliveira, Alexandra Teixeira, Jose M. Fernandes, Claudia Lopes, Alexandre Chicharo, Paulina Piairo, Lei Wu, Laura Rodriguez-Lorenzo, Lorena Dieguez, Sara Abalde-Cela
Summary: In the context of personalized medicine, the analysis of single cells is crucial for understanding cancer and providing accurate prognosis. This study combines surface-enhanced Raman scattering spectroscopy and droplet microfluidics to achieve multiplex phenotypic analysis of single cancer cells. It paves the way for the automated characterization of cell populations in cancer patients.
ADVANCED OPTICAL MATERIALS
(2023)
Article
Biochemistry & Molecular Biology
Giovanni Spiaggia, Patricia Taladriz-Blanco, Stefan Hengsberger, Dedy Septiadi, Christoph Geers, Aaron Lee, Barbara Rothen-Rutishauser, Alke Petri-Fink
Summary: Commercial static cell culture substrates have limited representation of in vivo biomechanical cues due to their inability to change physical properties over time. Gold nanoparticles have been used as transducers to overcome this limitation. In this study, gold nanorods were embedded in an elastomeric matrix to fabricate biocompatible light-responsive substrates. The analysis of the nanocomposite films showed a homogeneous heat distribution and increased stiffness when irradiated with NIR light. These films were biocompatible and cells remained viable, exhibiting a greater proliferation rate and stronger focal adhesion clustering.
Article
Nanoscience & Nanotechnology
Aaron Lee, Natalia Gosnell, Daela Milinkovic, Patricia Taladriz-Blanco, Barbara Rothen-Rutishauser, Alke Petri-Fink
Summary: Localized delivery of siRNA using electrostatic interactions has been achieved by assembling siRNA with cationic polyelectrolytes to create particle-decorated surfaces, which allows for spatial control of cell responses at biomaterial interfaces. The results demonstrate that cells in contact with the particle-decorated surface can survive, internalize particles, and undergo gene silencing. This study highlights the engineering potential of particle-decorated surfaces for delivering therapeutic payloads and guiding cell responses at biointerfaces.
ACS APPLIED BIO MATERIALS
(2023)
Article
Engineering, Biomedical
Manuela Estermann, Ricardo Coelho, Francis Jacob, Yen-Lin Huang, Ching-Yeu Liang, Ana Bela Faia-Torres, Dedy Septiadi, Barbara Drasler, Bedia Begum Karakocak, Irini Magdelina Dijkhoff, Alke Petri-Fink, Viola Heinzelmann-Schwarz, Barbara Rothen-Rutishauser
Summary: Reliable and predictive experimental models are urgently needed for studying the metastatic mechanisms of ovarian cancer cells in the omentum. The lack of certain omental cell types that influence cancer cell metastasis hinders the application of current tissue models. This study introduces a 3D multi-cellular human omentum tissue model that mimics the metastatic processes of ovarian cancer cells and allows the study of their interaction with endothelial cells and the effects of chemotherapeutic drugs. This model may serve as a platform for future patient-specific investigations in personalized oncology.
Article
Engineering, Environmental
Jessica Caldwell, Celine Loussert-Fonta, Gaelle Toullec, Niclas Heidelberg Lyndby, Beat Haenni, Patricia Taladriz-Blanco, Begona Espina, Barbara Rothen-Rutishauser, Alke Petri-Fink
Summary: Many researchers are studying the interaction between microplastics and marine fauna, aiming to understand exposure pathways and concentrations, as well as the impact of such interactions. This study focused on how Cassiopea andromeda jellyfish interacted with microplastics, using different analytical techniques. The results showed that the fluorescent microplastics could be successfully detected and their interaction with the jellyfish was likely influenced by the properties of the microplastics.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Article
Chemistry, Physical
Soukayna Belekbir, Mohammed El Azzouzi, Laura Rodriguez-Lorenzo, Adnane El Hamidi, Juan Arturo Santaballa, Moises Canle
Summary: One of the main challenges in photocatalysis is to find a stable and effective photocatalyst that works well under sunlight. In this study, the photocatalytic degradation of phenol in aqueous solution using TiO2-P25 impregnated with different concentrations of Co was investigated. Co(0.1%)/TiO2 showed the highest effectiveness under NUV-Vis irradiation, with approximately 96% TOC removal. UV radiation, on the other hand, only achieved 23% removal.
Article
Chemistry, Analytical
Juan Jose Lopez-Mayan, Blanca Alvarez-Fernandez, Elena Pena-Vazquez, Maria Carmen Barciela-Alonso, Antonio Moreda-Pineiro, Julie Maguire, Mick Mackey, Monica Quarato, Ivone Pinheiro, Begona Espina, Laura Rodriguez-Lorenzo, Pilar Bermejo-Barrera
Summary: A study was conducted to investigate the bioaccumulation of citrate-coated titanium dioxide nanoparticles in red (Palmaria palmata) and green (Ulva sp.) seaweed. The results showed that Ulva sp. had higher titanium concentrations than Palmaria palmata under the same exposure conditions. The titanium concentration in Ulva sp. reached its maximum after 28 days of exposure to 1.0 mgL(-1) of 5 nm TiO(2)NPs. The sizes and concentrations of TiO(2)NPs determined by SP-ICP-MS were similar for both seaweeds, suggesting that the titanium is mainly accumulated in Ulva sp. as ionic titanium or nanoparticles smaller than the limit of detection in size.
Article
Polymer Science
Sara Pereira, Ana Costa-Ribeiro, Pilar Teixeira, Laura Rodriguez-Lorenzo, Marta Prado, Miguel A. Cerqueira, Alejandro Garrido-Maestu
Summary: Chitosan, derived from chitin, possesses antimicrobial activity. Its application as micro- or nanoparticles expands its usage. However, the size of chitosan nanoparticles produced from different starting materials affects their antimicrobial properties.
Article
Chemistry, Multidisciplinary
Ivone Pinheiro, Monica Quarato, Antonio Moreda-Pineiro, Ana Vieira, Virginie Serin, David Neumeyer, Nicolas Ratel-Ramond, Sebastien Joulie, Alain Claverie, Miguel Spuch-Calvar, Miguel A. Correa-Duarte, Alexandre Campos, Jose Carlos Martins, Pilar Bermejo-Barrera, Marisa P. Sarria, Laura Rodriguez-Lorenzo, Begona Espina
Summary: Commercial ATO nanoparticles are relatively harmless to zebrafish and marine mussels, but they do affect the developmental parameters of zebrafish. Further research is needed on the accumulation of nanoparticles in the human body.
Article
Materials Science, Multidisciplinary
Ana Vieira, Laura Rodriguez-Lorenzo, Alejandro Garrido-Maestu, Begona Espina, Marilia Barreiros dos Santos
Summary: This study evaluated two main approaches, namely chemical modification and physical modification, for reducing fouling on polyethylene (PE) surfaces. The results showed that chitosan nanoparticles from different sources and micro-topography modification effectively reduced bacterial adhesion. These strategies offer eco-friendly and cost-effective solutions for preventing fouling and extending the lifetime of polymeric surfaces for environmental applications.
ADVANCED MATERIALS TECHNOLOGIES
(2023)
Article
Chemistry, Multidisciplinary
Giulia Mirabello, Lukas Steinmetz, Christoph Geers, Barbara Rothen-Ruthishauser, Mathias Bonmarin, Alke Petri-Fink, Marco Lattuada
Summary: This work demonstrates the application of lock-in thermography (LIT) in quantifying the concentration of gold nanoparticles embedded in polyvinyl alcohol (PVA) films. The study used films with different concentrations and sizes of gold nanoparticles and quantitatively analyzed the concentration by measuring the amplitude of the thermal signal emitted by the nanoparticles. The results showed that LIT is a reliable and non-destructive method to quantify nanoparticle concentrations and can detect concentration gradients in samples.
NANOSCALE ADVANCES
(2023)
Article
Engineering, Environmental
Jessica Caldwell, Alke Petri-Fink, Celine Loussert-Fonta, Gaelle Toullec, Niclas Heidelberg Lyndby, Beat Haenni, Patricia Taladriz-Blanco, Barbara Rothen-Rutishauser
Summary: Many researchers are studying the interaction between microplastics and marine fauna and working to monitor exposure pathways, concentrations, and their impacts. This study focuses on Cassiopea andromeda jellyfish, which live in (sub-)tropical coastal regions that are potentially contaminated by plastic waste. Fluorescent microplastics were detected using an optimized analytical protocol, and their interaction with the jellyfish was found to be influenced by the properties of the microplastics, such as density and hydrophobicity.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Review
Radiology, Nuclear Medicine & Medical Imaging
Chiara Pozzessere, Christophe von Garnier, Catherine Beigelman-Aubry
Summary: Lung cancer screening programs using low-dose Computed Tomography (LDCT) are being implemented globally, but concerns over radiation exposure and potential risks may impact participation. This review aims to assess the radiation risk from LDCT in the screening program, providing estimates of cumulative dose and risk to improve awareness and attendance. International trials and factors influencing the radiation risk will be summarized, and future directions for personalized screening programs and dose reduction will be discussed.
Article
Chemistry, Multidisciplinary
Jessica Caldwell, Patricia Taladriz-Blanco, Laura Rodriguez-Lorenzo, Barbara Rothen-Rutishauser, Alke Petri-Fink
Summary: The presence of submicron- and nanoplastic particles has attracted increasing interest, but there are few analytical techniques capable of detecting these small plastic particles at low concentrations. This study investigates the potential of gold nanostar-based SERS substrates for the detection of submicron- and nanoplastic particles. The results demonstrate the promising potential of these substrates for nanoplastic detection, with variations in analytical performance depending on the properties of the plastics being studied.
ENVIRONMENTAL SCIENCE-NANO
(2023)
Article
Nanoscience & Nanotechnology
Daniela Rubanova, Svitlana Skoroplyas, Alena Libanska, Eva Randarova, Josef Bryja, Michaela Chorvatova, Tomas Etrych, Lukas Kubala
Summary: This study explored the use of nanocarriers to deliver dexamethasone for treating rheumatoid arthritis. The results showed that this method increased the accumulation of dexamethasone in the arthritic area, had a dose-dependent healing effect, and reduced the accumulation of the RA mediator RANKL. This study provides a feasible strategy for improving rheumatoid arthritis therapy.
NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
(2024)
Article
Nanoscience & Nanotechnology
Thanpisit Lomphithak, Apiwit Sae-Fung, Simone Sprio, Anna Tampieri, Siriporn Jitkaew, Bengt Fadeel
Summary: In this study, iron-doped hydroxyapatite (FeHA) was evaluated as a potential nanomedicine approach for treating PDAC. The results showed that FeHA, in combination with a sublethal dose of the GPX4 inhibitor RSL3, could trigger ferroptosis in KRAS mutant PANC-1 cells while sparing normal human cells. The induction of ferroptosis by FeHA plus RSL3 was found to be dependent on the metalloreductase STEAP3 in PDAC cells.
NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
(2024)
Review
Nanoscience & Nanotechnology
Marco Felipe Salas-Orozco, Ana Cecilia Lorenzo-Leal, Idania de Alba Montero, Nuria Patino Marin, Miguel Angel Casillas Santana, Horacio Bach
Summary: The emergence of antibiotic-resistant bacteria in severe infections is a growing concern, particularly in hospital environments. Metal-based nanomaterials have shown potential as an alternative to combat these bacteria, but there is a risk of bacterial resistance and environmental accumulation.
NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
(2024)
