期刊
NANOMEDICINE
卷 10, 期 17, 页码 2643-2657出版社
FUTURE MEDICINE LTD
DOI: 10.2217/nnm.15.103
关键词
biocompatibility; caspases; Cathepsin-B; CD95 (APO-1/Fas); cell death; gold nanoparticles; lung epithelial cells; nanoparticle shape; nanotoxicology; reactive oxygen species
资金
- European Commission via ERA-NanoScience, project 'NanoTruck'
- Science Foundation Ireland [11/PI/1108]
- Spanish Ministry of Science and Innovation [MAT2011-26851-CO2-01]
- Fondo Social Europeo (FSE
- Gobierno de Aragon)
- Alexander von Humboldt Foundation
- European Respiratory Society [LTRF-MC1572-2010]
- Swiss National Science Foundation
- Adolphe Merkle Foundation
Aim: To investigate the influence of gold nanoparticle geometry on the biochemical response of Calu-3 epithelial cells. Materials & methods: Spherical, triangular and hexagonal gold nanoparticles (GNPs) were used. The GNP-cell interaction was assessed via atomic absorption spectroscopy (AAS) and transmission electron microscopy (TEM). The biochemical impact of GNPs was determined over 72 h at (0.0001-1 mg/ml). Results: At 1 mg/ml, hexagonal GNPs reduced Calu-3 viability below 60%, showed increased reactive oxygen species production and higher expression of proapoptotic markers. A cell mass burden of 1: 2: 12 as well as number of GNPs per cell (2: 1: 3) was observed for spherical: triangular: hexagonal GNPs. Conclusion: These findings do not suggest a direct shape-toxicity effect. However, do highlight the contribution of shape towards the GNP-cell interaction which impacts upon their intracellular number, mass and volume dose.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据