期刊
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
卷 125, 期 6, 页码 1261-1268出版社
MOSBY-ELSEVIER
DOI: 10.1016/j.jaci.2010.03.018
关键词
TRAIL; psoriasis; dendritic cell; inflammation; CCL20; Toll-like receptor 1; Toll-like receptor 2; S100A12; CD32
资金
- National Institutes of Health (NIH), National Center for Research Resources (NCRR) [UL1 RR024143]
- NIH [UL1 RR024143, GM07739]
- Dana Foundation
- Rockefeller University
- Doris Duke Charitable Foundation
- Amgen
- [1 K23 AR052404-01A1]
- NATIONAL CENTER FOR RESEARCH RESOURCES [UL1RR024143] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF ARTHRITIS AND MUSCULOSKELETAL AND SKIN DISEASES [K23AR052404] Funding Source: NIH RePORTER
Background: Previous work has identified CD11c(+)CD1c(-) dendritic cells (DCs) as the major inflammatory dermal DC population in patients with psoriasis vulgaris and CD1c(+) DCs as the resident cutaneous DC population. Objective: We sought to further define molecular differences between these 2 myeloid dermal DC populations. Methods: Inflammatory and resident DCs were single-cell sorted from lesional skin biopsy specimens of patients with psoriasis, and the transcriptome of CD11c(+)CD1c(-) versus CD1c(+) DCs was determined. Results were confirmed with RT-PCR, flow cytometry, immunohistochemistry, and double-labeled immunofluorescence. Human keratinocytes were cultured for functional studies. Results: TNF-related apoptosis-inducing ligand (TRAIL), Toll-like receptors 1 and 2, S1000A12/ENRAGE, CD32, and many other inflammatory products were differentially expressed in inflammatory DCs compared with resident DCs. Flow cytometry and immunofluorescence confirmed higher protein expression on CD1c(-) versus CD1c(+) DCs. TRAIL receptors, death receptor 4, and decoy receptor 2 were expressed in keratinocytes and dermal cells. In vitro culture of keratinocytes with TRAIL induced CCL20 chemokine. Conclusions: CD11c(+)CD1c(-) inflammatory DCs in psoriatic lesional skin express a wide range of inflammatory molecules compared with skin-resident CD1c(+) DCs. Some molecules made by inflammatory DCs, including TRAIL, could have direct effects on keratinocytes or other skin cell types to promote disease pathogenesis. (J Allergy Clin Immunol 2010;125:1261-8.)
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据