期刊
NANOMEDICINE
卷 10, 期 16, 页码 2513-2525出版社
FUTURE MEDICINE LTD
DOI: 10.2217/NNM.15.95
关键词
apoptosis; cell cycle; cell delivery; cell proliferation; c-myc; gene knockdown; gold nanoparticles; knockdown; nanoparticles; off-target effects; proliferation; RNAi; siRNA
资金
- EPSRC
- EPSRC [EP/H006885/1] Funding Source: UKRI
- Engineering and Physical Sciences Research Council [EP/H006885/1] Funding Source: researchfish
Aims: RNAi is a powerful tool for gene silencing that can be used to reduce undesirable overexpression of oncogenes as a novel form of cancer treatment. However, when using RNAi as a therapeutic tool there is potential for associated gene effects. This study aimed to utilize gold nanoparticles to deliver siRNA into HeLa cells. Results: Knockdown of the c-myc oncogene by RNAi, at the RNA, protein and cell proliferation level was achieved, while also identifying associated gene responses. Discussion: The gold nanoparticles used in this study present an excellent delivery platform for siRNA, but do note associated gene changes. Conclusion: The study highlights the need to more widely assess the cell physiological response to RNAi treatment, rather than focus on the immediate RNA levels.
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