期刊
NANO RESEARCH
卷 9, 期 2, 页码 291-305出版社
TSINGHUA UNIV PRESS
DOI: 10.1007/s12274-015-0909-5
关键词
silica nanoparticles; gene delivery; plasmid DNA; particle sizes; cellular uptake
类别
资金
- Australian Research Council
- Australian Microscopy & Microanalysis Research Facility at the Centre for Microscopy and Microanalysis
- University of Queensland
- Queensland node of the Australian National Fabrication Facility (ANFF)
Silica-based nanoparticles are promising carriers for gene delivery applications. To gain insights into the effect of particle size on gene transfection efficiency, amine-modified monodisperse Stober spheres (NH2-SS) with diameters of 125, 230, 330, 440, and 570 nm were synthesized. The in vitro transfection efficiencies of NH2-SS for delivering plasmid DNA encoding green fluorescent protein (GFP) (pcDNA3-EGFP, abbreviated as pcDNA, 6.1 kbp) were studied in HEK293T cells. NH2-SS with a diameter of 330 nm (NH2-SS330) showed the highest GFP transfection level compared to NH2-SS particles with other sizes. The transfection efficiency was found as a compromise between the binding capacity and cellular uptake performance of NH2-SS330 and pcDNA conjugates. NH2-SS330 also demonstrated the highest transfection efficiency for plasmid DNA (pDNA) with a bigger size of 8.9 kbp. To our knowledge, this study is the first to demonstrate the significance of particle size for gene transfection efficiency in silica-based gene delivery systems. Our findings are crucial to the rational design of synthetic vectors for gene therapy.
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