期刊
NANO LETTERS
卷 15, 期 7, 页码 4467-4471出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.nanolett.5b00852
关键词
Nucleic acid; machines; molecular devices; pH; DNA nanotechnology; triplex
类别
资金
- European Research Council, ERC [336493]
- Associazione Italiana per la Ricerca sul Cancro, AIRC [14420]
- International Research Staff Exchange Scheme (IRSES) grant under the Marie Curie Actions program
- Natural Sciences and Engineering Research Council of Canada (NSERC) [2014-06403]
Inspired by naturally occurring pH-regulated receptors, here we propose a rational approach to introduce pH-induced allostery into a wide range of DNA-based receptors. To demonstrate this we re-engineered two model DNA-based probes, a molecular beacon and a cocaine-binding aptamer, by introducing in their sequence a pH-dependent domain. We demonstrate here that we can finely tune the affinity of these model receptors and control the load/release of their specific target molecule by a simple pH change.
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