期刊
NANO LETTERS
卷 15, 期 8, 页码 5539-5544出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.nanolett.5b02123
关键词
DNA nanotechnology; self-assembly; hybridization chain reaction; pH; DNA nanostructures
类别
资金
- European Research Council, ERC [336493]
- Associazione Italiana per la Ricerca sul Cancro, AIRC [14420]
- Int. Research Staff Exchange Scheme (IRSES)
- Natural Sciences and Engineering Research Council of Canada (NSERC) [436381-2013]
By taking inspiration from nature, where selforganization of biomolecular species into complex systems is finely controlled through different stimuli, we propose here a rational approach by which the assembly and disassembly of DNA-based concatemers can be controlled through pH changes. To do so we used the hybridization chain reaction (HCR), a process that, upon the addition of an initiator strand, allows to create DNA-based concatemers in a controlled fashion. We re-engineered the functional units of HCR through the addition of pH-dependent clamp-like triplex-forming domains that can either inhibit or activate the polymerization reaction at different pHs. This allows to finely regulate the HCR-induced assembly and disassembly of DNA concatemers at either basic or acidic pHs in a reversible way. The strategies we present here appear particularly promising as novel tools to achieve better spatiotemporal control of self-assembly processes of DNA-based nanostructures.
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