期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 62, 期 23, 页码 5237-5247出版社
AMER CHEMICAL SOC
DOI: 10.1021/jf5007775
关键词
pyrimethanil; sublethal effects; toxicogenomics; toxicity mechanisms; mode of action; Saccharomyces cerevisiae
资金
- European Funds for Regional Development-FEDER through the Competitiveness Factors Operational Programme COMPETE
- National Funds through Foundation for Science and Technology, Portugal [PTDC/AMB/64230/2006, PTDC/AAC-AMB/111317/2009, PEst-OE/EQB/LA0023/2011, SFRH/BD/60933/2009]
- Fundação para a Ciência e a Tecnologia [PTDC/AMB/64230/2006, SFRH/BD/60933/2009, PTDC/AAC-AMB/111317/2009] Funding Source: FCT
Pyrimethanil is a fungicide mostly applied in vineyards. When misused, residue levels detected in grape must or in the environment may be of concern. The present work aimed to analyze mechanisms underlying response to deleterious effects of pyrimethanil in the eukaryotic model Saccharomyces cerevisiae. Pyrimethanil concentration-dependent effects at phenotypic (inhibition of growth) and transcriptomic levels were examined. For transcriptional profiling, analysis focused on two sublethal exposure conditions that inhibited yeast growth by 20% or 50% compared with control cells not exposed to the fungicide. Gene expression modifications increased with the magnitude of growth inhibition, in numbers and fold-change of differentially expressed genes and in diversity of over-represented functional categories. These included mostly biosynthesis of arginine and sulfur amino acids metabolism, as well as energy conservation, antioxidant response, and multidrug transport. Several pyrimethanil-responsive genes encoded proteins sharing significant homology with proteins from phytopathogenic fungi and ecologically relevant higher eukaryotes.
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