Article
Biochemistry & Molecular Biology
Jamie M. Newton, Emma L. Betts, Lyto Yiangou, Jose Ortega Roldan, Anastasios D. Tsaousis, Gary S. Thompson
Summary: Blastocystis is an opportunistic parasite commonly found in the intestines of humans and other animals, but there is limited knowledge about its biology. Analyzing its metabolites can reveal its role in health and disease. Using methanol as an extraction solvent and bead bashing as a lysis technique, carrying out the procedure at room temperature provides a consistent and efficient method to extract metabolites from Blastocystis for NMR analysis.
Article
Food Science & Technology
Marius Gheorghe Miricioiu, Roxana Elena Ionete, Diana Costinel, Oana Romina Botoran
Summary: The H-1-NMR carbohydrates profiling was used to discriminate fruits from Rosaceae family in terms of botanical origin and harvest year. Multivariate data analysis, such as PCA, LDA, and Pearson analysis, was applied for classification. The study also explored the potential association with relevant climatic data.
Article
Biochemistry & Molecular Biology
Martha E. Garcia-Aguilera, Ronna Delgado-Altamirano, Nayelli Villalon, Francisco Ruiz-Teran, Mariana M. Garcia-Garnica, Iran Ocana-Rios, Eduardo Rodriguez de San Miguel, Nuria Esturau-Escofet
Summary: Wine is sensitive to temperature, light, and oxygen, which can modify its physicochemical characteristics and metabolomic fingerprinting. In this study, different chemometric methods (PCA, ASCA, and PARAFAC) were used to analyze 1H-NMR spectra variations of red wine samples stored at different temperatures and time. The chemometric analyses successfully tracked changes related to wine sample, temperature, and time, and identified important metabolites showing significant variations.
Article
Chemistry, Multidisciplinary
Ziqing Wang, Simone Pisano, Veronica Ghini, Pavel Kaderavek, Milan Zachrdla, Philippe Pelupessy, Morgan Kazmierczak, Thorsten Marquardsen, Jean-Max Tyburn, Guillaume Bouvignies, Giacomo Parigi, Claudio Luchinat, Fabien Ferrage
Summary: Metabolomics is a systematic approach to study metabolites in biological systems, providing information about metabolism and diseases. Metabolites play essential roles in biological processes, interacting with macromolecules, but methods to study these interactions systematically are currently limited.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Article
Physiology
Ioannis Georgoulis, Christian Bock, Gisela Lannig, Hans O. Portner, Inna M. Sokolova, Konstantinos Feidantsis, Ioannis A. Giantsis, Basile Michaelidis
Summary: This study investigates the stress memory effect of heat hardening on M. galloprovincialis metabolite profile, aiming to identify the underlying biochemical mechanisms that enhance mussels' thermal tolerance.
FRONTIERS IN PHYSIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Laela Hayu Nurani, Abdul Rohman, Anjar Windarsih, Any Guntarti, Florentinus Dika Octa Riswanto, Endang Lukitaningsih, Nurrulhidayah Ahmad Fadzillah, Mohamad Rafi
Summary: The study on the authentication of Curcuma species using H-1-NMR spectroscopy and chemometrics has demonstrated the importance of metabolite fingerprinting in the authentication of herbal and medicinal plants.
Article
Biochemistry & Molecular Biology
Luke Hipperson, William J. Hadden, Christopher B. Nahm, Anthony J. Gill, Jaswinder S. Samra, Anthony Dona, Anubhav Mittal, Sumit Sahni
Summary: A panel of three metabolites can stratify PDAC patients into good or poor prognosis groups based on overall survival. Patients with abnormal levels of 2 or more metabolites in their urine have significantly lower survival rates. Using urinary metabolites as prognostic biomarkers for PDAC patients shows promise for future research and development.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2021)
Article
Endocrinology & Metabolism
Sara Cardoso, Debora Cabral, Marcelo Maraschin, Miguel Rocha
Summary: Automated and accurate identification of metabolites in 1D H-1-NMR samples remains a challenge, with most tools focusing on metabolite quantification rather than identification. This study developed novel methods for metabolite annotation using a vast library of reference 1D H-1-NMR peak lists. The proposed method, NMRFinder, outperformed previous tools by accurately annotating metabolites from samples regardless of acquisition conditions.
Article
Food Science & Technology
Qiuyu Lan, Silvia Tappi, Giacomo Braschi, Gianfranco Picone, Pietro Rocculi, Luca Laghi
Summary: This study investigated the effects of high hydrostatic pressure (HHP) on the metabolome of striped prawn during chilled storage, and found that HHP treatment improved the freshness and taste quality of the prawn, while extending the storage period.
Article
Chemistry, Analytical
Daniel H. Lysak, William W. Wolff, Ronald Soong, Wolfgang Bermel, Eriks Kupce, Amy Jenne, Rajshree Ghosh Biswas, Daniel Lane, Genevieve Gasmi-Seabrook, Andre Simpson
Summary: Many important biological components contain nitrogen. However, N-15 nuclei are rarely used in NMR-based metabolite assignment due to their low abundance and the lack of comprehensive databases. In this study, a broadband N-15-edited H-1-C-13 HSQC NMR experiment was developed to identify nitrogen-containing metabolites based on their C-13, H-1, and N-15 chemical shifts.
ANALYTICAL CHEMISTRY
(2023)
Article
Plant Sciences
Nathan Bryant, Wellington Muchero, Rachel A. Weber, Jaime Barros, Jin-Gui Chen, Timothy J. Tschaplinski, Yunqiao Pu, Arthur J. Ragauskas
Summary: The study found that the lignin content in the cell wall of Populus was significantly increased in the region infected by Septoria, and the composition of lignin in the infected region was also different. These results provide important insights into the wood characteristic changes of Populus during Septoria infection.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Cell Biology
Sneider Alexander Gutierrez Guarnizo, Zemfira N. Karamysheva, Elkin Galeano, Carlos E. Muskus
Summary: Leishmania parasites causing leishmaniasis exhibit high drug resistance, which poses a major threat to public health. This study demonstrates that metabolomic profiling can potentially predict the response level of antimony-resistant parasites, as well as identify differences in metabolite composition and suggest metabolic remodeling to combat the drug. The findings also indicate a link between metabolite composition changes and optimized response to stress, and highlight potential metabolite biomarkers for drug resistance.
Article
Biochemistry & Molecular Biology
Doyoung Kwon, Wonho Lee, Sou Hyun Kim, Young-Suk Jung
Summary: This study investigated the metabolic characteristics of infant and adult mouse livers using H-1-NMR spectroscopy. The results revealed differences in hepatic metabolite concentrations between infant and adult mice, which may be attributed to their developmental stages and dietary sources.
Article
Plant Sciences
Aamir Niaz, Ahmad Adnan, Rashida Bashir, Muhammad Waseem Mumtaz, Syed Ali Raza, Umer Rashid, Chin Ping Tan, Tai Boon Tan
Summary: The ethyl acetate fraction of Tamarix dioica extract showed strong antioxidant activity and anti-diabetic potential, possibly due to the presence of metabolites such as quercetin and catechin. This study confirmed the ethnopharmacological use of T. dioica in managing diabetes mellitus and suggested a natural approach for anti-hyperglycemia.
Article
Chemistry, Multidisciplinary
Harishkumarreddy Patnam, Sontyana Adonijah Graham, Punnarao Manchi, Mandar Vasant Paranjape, Jae Su Yu
Summary: Triboelectric nanogenerators (TENGs) have been highly promoted to solve energy-related issues. In this study, an eco-friendly pectin polymer was used to fabricate a TENG with excellent output performance.
Review
Food Science & Technology
Rebekka Thogersen, Hanne Christine Bertram
Summary: Studies suggest that incorporating food compounds with beneficial effects on colon health into processed meat products may mitigate potential harmful effects in the gastrointestinal tract, thereby offering a strategy to develop healthier meat products.
TRENDS IN FOOD SCIENCE & TECHNOLOGY
(2021)
Article
Microbiology
Louise Margrethe Arildsen Jakobsen, Ulrik Kraemer Sundekilde, Henrik Jorgen Andersen, Witold Kot, Josue Leonardo Castro Mejia, Dennis Sandris Nielsen, Axel Kornerup Hansen, Hanne Christine Bertram
Summary: The study found that a diet high in BMO did not have a significant impact on the gut microbiota and metabolic activity in mice, highlighting the importance of the presence of bifidobacteria for microbial effects of milk oligosaccharides in the gastrointestinal tract.
Article
Nutrition & Dietetics
Louise Kjolbaek, Jesper M. Schmidt, Emilien Rouy, Klaus J. Jensen, Arne Astrup, Hanne C. Bertram, Marianne Hammershoj, Anne Raben
Summary: This study investigated the effects of different dairy matrices on postprandial lipid, glycemic, and appetite responses over 8 hours. The results showed that different dairy products with varying matrices had an impact on lipid responses, but had minimal effects on apoB-48 responses.
AMERICAN JOURNAL OF CLINICAL NUTRITION
(2021)
Article
Chemistry, Multidisciplinary
Weiwei He, Louise M. A. Jakobsen, Line F. Zachariassen, Axel K. Hansen, Henrik J. Andersen, Hanne Christine Bertram
Summary: This study used nuclear magnetic resonance (NMR) to investigate the potential gut-bone axis by linking NMR data on bone mineralization with NMR profiling of gut metabolites. The results showed a strong correlation between T-2 relaxation and mechanical strength of the bone, as well as a correlation between gut short-chain fatty acids and bone T-2 populations. This suggests the potential existence of a gut-bone axis.
MAGNETIC RESONANCE IN CHEMISTRY
(2022)
Article
Food Science & Technology
Weiwei He, Zhuqing Xie, Rebekka Thogersen, Martin Kroyer Rasmussen, Line F. Zachariassen, Niklas Rye Jorgensen, Jan Voerum Norgaard, Henrik J. Andersen, Dennis S. Nielsen, Axel K. Hansen, Hanne Christine Bertram
Summary: This study explores nutritional strategies to counteract bone demineralization in ovarierectomized rats. Calcium supplementation improves bone mineral density and strength, while inulin and lactose show beneficial effects on bone mineralization. The study also reveals the modulation effect of calcium on gut microbiome composition and function.
MOLECULAR NUTRITION & FOOD RESEARCH
(2022)
Article
Chemistry, Applied
Soren D. Nielsen, Louise M. A. Jakobsen, Nina R. W. Geiker, Hanne Christine Bertram
Summary: Consuming different dairy products has varying effects on human health, with fermented yogurt showing higher bioactivity potential than chemically acidified yogurt and whole milk. Cold storage and heat-inactivation affect the bioactivity potential of yogurt.
Article
Endocrinology & Metabolism
Weiwei He, Tine Vrist Dam, Rebekka Thogersen, Mette Hansen, Hanne Christine Bertram
Summary: This study found that the menstrual cycle and use of oral contraceptives can affect levels of metabolites and bone turnover markers in women. During the menstrual cycle, plasma levels of certain amino acids vary significantly and are correlated with fluctuations in progesterone and estrogen levels. Additionally, users of oral contraceptives have lower levels of metabolites and bone turnover markers compared to nonusers.
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2022)
Editorial Material
Chemistry, Multidisciplinary
Hanne Christine Bertram, John van Duynhoven
MAGNETIC RESONANCE IN CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Rasmus Espersen, Banny Silva Barbosa Correia, Lars Rejnmark, Hanne Christine Bertram
Summary: This study investigated the diurnal changes in the blood metabolome and the effects of vitamin D-3 supplementation. The results showed diurnal effects on the majority of the quantified metabolites, with a change in the overall plasma metabolome around 12 AM. Vitamin D supplementation had a minor influence on the blood metabolome but significantly affected plasma acetone levels.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Food Science & Technology
Weiwei He, Zhuqing Xie, Nina Kolln Wittig, Line F. Zachariassen, Amanda Andersen, Henrik J. Andersen, Henrik Birkedal, Dennis S. Nielsen, Axel K. Hansen, Hanne Christine Bertram
Summary: Evidence suggests that yogurt, as a source of calcium, can increase spine bone density and is associated with changes in gut microbiome and glycine-related pathways. However, adding inulin to yogurt does not affect bone mineralization in ovariectomized rats.
MOLECULAR NUTRITION & FOOD RESEARCH
(2022)
Article
Food Science & Technology
Weiwei He, Hanne Christine Bertram
Summary: This review discusses the importance of using nuclear magnetic resonance (NMR)-based metabolomics in food and nutrition science to gain a better understanding of the impact of food and food components on gut health.
Article
Nutrition & Dietetics
Sofie Kaas Lanng, Mikkel Oxfeldt, Simon Stjernholm Pedersen, Frank Ted Johansen, Jeyanthini Risikesan, Trine Lejel, Hanne Christine Bertram, Mette Hansen
Summary: This study aimed to compare the effects of ingesting different protein sources on amino acid levels in the blood and activation of the mTORC1 signaling pathway in skeletal muscle. The results showed that whey protein had a significantly higher concentration of amino acids compared to cricket and pea protein, while activation of the mTORC1 signaling pathway did not differ between protein sources at rest and after exercise.
EUROPEAN JOURNAL OF NUTRITION
(2023)
Article
Food Science & Technology
Xiaorui Zhao, Weiwei He, Louise M. A. Jakobsen, Line F. Zachariassen, Axel K. Hansen, Martin Kroyer Rasmussen, Hanne Christine Bertram
Summary: This study investigates the effects of prebiotics on the metabolic activity of the gut microbiome and the impact of dietary calcium depletion on the liver metabolome and transcriptome. The results show that prebiotic ingestion leads to changes in circulating metabolites and affects liver metabolism and gene expression. This suggests that prebiotics can regulate liver function and influence overall metabolism.
MOLECULAR NUTRITION & FOOD RESEARCH
(2023)
Article
Gastroenterology & Hepatology
Oyekunle John Oladosu, Banny Silva Barbosa Correia, Beatrice Grafl, Dieter Liebhart, Cornelia C. Metges, Hanne Christine Bertram, Gurbuz Das
Summary: This study investigated the effects of gut pathogens A. galli and H. gallinarum on the plasma and liver metabolome of chickens. The results showed that infected hens had significantly elevated concentrations of various metabolites in the plasma, while there were no significant differences in liver metabolites. The alterations in plasma metabolites mainly occurred during the pre-patent period of infections.
Article
Biochemistry & Molecular Biology
Louise M. Arildsen Jakobsen, Weiwei He, Nicholas Ditzel, Marianne Danielsen, Trine K. Dalsgaard, Niklas Rye Jorgensen, Peter Bollen, Hanne C. Bertram
Summary: This study investigated the effects of whey protein complexation of vitamin D-3 supplements on bone health in rats. The results showed that complexation did not improve the bioavailability of vitamin D-3 or have any significant impact on bone strength or microstructure.