期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 61, 期 20, 页码 4822-4830出版社
AMER CHEMICAL SOC
DOI: 10.1021/jf401139u
关键词
mycotoxins; green coffee bean extracts; dietary supplements; liquid chromatography-mass spectrometry
资金
- Center for Food Safety and Applied Nutrition
An ultrahigh-performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) method for the determination of 34 mycotoxins in dietary supplements containing green coffee bean (GCB) extracts was developed, evaluated, and used in the analysis of 50 commercial products. A QuEChERS-like procedure was used for isolation of target analytes from the examined matrices. Average recoveries of the analytes were in the range of 75-110%. The precision of the method expressed as relative Standard deviation was below 12%. Limits of detection (LODs) and limits of quantitation (LOQs) ranged from 1.0 to 50.0 mu g/kg and from 2.5 to 100 mu/kg, respectively. Due to matrix effects, the method of standard additions was used to ensure accurate quantitation. Ochratoxin A, ochratoxin B, fumonisin B1 and mycophenolic acid were found in 36%, 32%, 10%, and 16% of tested products, respectively. Mycotoxins occurred in the following concentration ranges: ochratoxin A, <1.0-136.9 mu g/kg; ochratoxin B, <1.0-20.2 mu g/kg; fumonisin B-1, <50.0-415.0 mu g/kg; mycophenolic acid, <5.0-395.0 mu g/kg. High-resolution mass spectrometry operated in full MS and MS/MS mode was used to confirm the identities of the reported compounds.
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