Analysis of Phospholipids in Infant Formulas Using High Performance Liquid Chromatography-Tandem Mass Spectrometry

Phospholipids have been used widely in the food industry as emulsifiers, but it is their biological and nutritional function that has been the focus over recent years. The recognition of the importance of phospholipids and sphingomyelin for infant development has led to an increase in the number of infant formulas claiming to contain these complex lipid components. Therefore, the ability to measure these lipids in infant formulas and dairy-derived complex lipid ingredients for fortification purposes is important. A high-performance liquid chromatography-tandem mass spectrometry method that quantifies phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol, phosphatidylserine, and sphingomyelin found in infant formulas and dairy-derived complex lipid ingredients is described. The method uses external standards of similar fatty acid profile for calibration. The recovery of phospholipids ranged from 92% to 102% with a method repeatability coefficient of variation of 6-10%. In addition to the specificity and selectivity of the method, details of the molecular species in the individual phospholipid classes are available using this method.