Article
Biochemistry & Molecular Biology
Ya-Ling Yang, Ming-Chao Tsai, Yen-Hsiang Chang, Chen-Chen Wang, Pei-Yi Chu, Hung-Yu Lin, Ying-Hsien Huang
Summary: This study demonstrates the inhibitory effect of MIR29A on LOX, LOXL2, and VEGFA by targeting their 3' UTRs, leading to promotion of apoptosis and reduction in cell viability and wound healing. The results confirm that MIR29A-3p mimic can directly bind to the 3' UTRs of LOX and VEGFA to downregulate their gene and protein expression levels.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Xiang Chen, Margaret Clement, M. John Hicks, Poonam Sarkar, M. Waleed Gaber, Tsz-Kwong Man
Summary: This study found that lysyl oxidase (LOX) expression was associated with cytoplasmic mislocalization of p27 in osteosarcoma. Higher LOX expression promoted cell proliferation, migration, and invasiveness, and resulted in pulmonary metastases. The upregulation of phospho-FAK was identified as the mechanism by which LOX promotes osteosarcoma metastasis. FAK inhibition may be a promising therapeutic strategy to reduce LOX-mediated metastasis in osteosarcoma.
Article
Gastroenterology & Hepatology
Nan Zhao, Chen Chen, Yuhong Guo, Tieju Liu, Na Che, Danfang Zhang, Xiaohui Liang, Yanhui Zhang, Xiulan Zhao
Summary: This study aimed to explore the relationship between LOXL2 and immune infiltration and vasculogenic mimicry (VM) and to identify the role of LOXL2 in HCC diagnosis and prognosis evaluation. The findings showed that LOXL2 expression was higher in HCC than in normal tissues, and high LOXL2 expression was associated with a poorer prognosis of HCC. LOXL2 is involved in immune cell infiltration and promotes VM by upregulating IQGAP1, making it a potential biomarker for HCC diagnosis and prognosis prediction.
DIGESTIVE AND LIVER DISEASE
(2023)
Article
Materials Science, Multidisciplinary
Yi Shan, Nilofer Sayed, Kok Chan Chong, Hui Jun Ting, Xianglong Liu, Bowen Li, Jingjing Liu, Min Wu, Jiong-Wei Wang, Bin Liu
Summary: Nonalcoholic steatohepatitis (NASH) is a common disease worldwide, with potential progression to fibrosis, cirrhosis, and hepatocellular carcinoma. This study developed an activatable imaging probe TPAH to monitor fibrosis progression in NASH, showing promising specificity.
ACS MATERIALS LETTERS
(2023)
Article
Cell Biology
Qipeng Wu, Le Li, Chunmeng Miao, Muhammad Hasnat, Lixin Sun, Zhenzhou Jiang, Luyong Zhang
Summary: This study demonstrates that osteopontin (OPN) promotes hepatocellular carcinoma (HCC) progression by increasing reactive oxygen species (ROS) production through upregulating the expression of NADPH oxidase 1 (NOX1) and activating the JAK2/STAT3 signaling pathway. Disruption of the OPN/NOX1 axis might be a promising therapeutic strategy for HCC.
CELL DEATH & DISEASE
(2022)
Article
Oncology
Jozafina Haj-Shomaly, Avital Vorontsova, Tamar Barenholz-Cohen, Oshrat Levi-Galibov, Mahesh Devarasetty, Michael Timaner, Ziv Raviv, Tim J. Cooper, Shay Soker, Peleg Hasson, Daphne Weihs, Ruth Scherz-Shouval, Yuval Shaked
Summary: Metastasis is a major cause of cancer-related deaths. This study found that chemotherapy can induce a host-mediated mechanism that promotes remodeling of the extracellular matrix (ECM), which facilitates cancer cell seeding and structural changes in the lungs. The research also revealed that the ECM remodeling is mediated by CD8 T cells expressing the enzyme lysyl oxidase (LOX). This study highlights the role of immune cells in regulating the ECM and metastasis following chemotherapy, suggesting that inhibiting chemotherapy-induced ECM remodeling may be a potential therapeutic strategy for metastatic cancer.
Review
Biochemistry & Molecular Biology
Philip C. Trackman, Yaser Peymanfar, Sayon Roy
Summary: Lysyl oxidases are multifunctional proteins involved in the biosynthesis and maturation of connective tissues. Their overexpression is associated with fibrosis and cancer, while their propeptide has tumor-suppressing effects. In diabetic retinopathy, lysyl oxidases are overexpressed and their propeptide levels are elevated. Further research is needed to understand the activities and mechanisms of lysyl oxidase propeptide.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Tatyana Liburkin-Dan, Inbal Nir-Zvi, Hila Razon, Ofra Kessler, Gera Neufeld
Summary: The five lysyl-oxidase genes were knocked out in MDA-MB-231 breast cancer cells to identify LOX-regulated genes and study their mechanism. Re-expression experiments revealed four downregulated genes rescued by LOX re-expression. LOX variants with point mutations induced gene expression, suggesting unrecognized functions of LOX pro-enzyme.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Sharad Khare, Tripti Khare, Raghu Ramanathan, Jamal A. Ibdah
Summary: This review discusses the role and involvement of microRNAs (miRs) in the heterogeneity and metastasis of hepatocellular carcinoma, with a special emphasis on their potential as diagnostic and prognostic tools in the risk prediction, early detection, and treatment of hepatocellular carcinoma.
Article
Dentistry, Oral Surgery & Medicine
Yujia Cui, Jing Xie, Linyi Cai, Demao Zhang, Jianxun Sun, Xuedong Zhou
Summary: This study suggests that berberine repairs bone destruction caused by apical periodontics by reducing osteoclast activity, promoting osteoblast differentiation, and inhibiting inflammatory response.
Article
Oncology
Rui Wang, Zhijian Xu, Jiaping Tian, Qian Liu, Jingwen Dong, Lijuan Guo, Boning Hai, Xia Liu, Hangping Yao, Zhi Chen, Junjie Xu, Lijun Zhu, Haiyi Chen, Tingjun Hou, Weiliang Zhu, Jimin Shao
Summary: Pterostilbene is identified as a potent RR inhibitor targeting RRM2, which can effectively inhibit both HCC proliferation and HBV replication, showing potential for the treatment of HCC and HBV-related HCC.
AMERICAN JOURNAL OF CANCER RESEARCH
(2021)
Review
Biochemistry & Molecular Biology
Sandra Ferreira, Nuno Saraiva, Patricia Rijo, Ana S. Fernandes
Summary: LOX and LOXL2 are amine oxidases that catalyze cross-linking reactions of elastin and collagen in connective tissue, with LOXL2 showing high expression in certain tumors. Inhibition of LOX and LOXL2 is considered a promising strategy for preventing metastasis and invasion of breast cancer. Novel synthetic compounds acting as selective inhibitors for LOX/LOXL2 or dual LOX/LOXL inhibitors have been developed, showing potential for effective therapeutic approaches in breast cancer treatment.
Article
Biochemistry & Molecular Biology
Zengliang Li, Liang Shi, Xiangwei Li, Xiaopeng Wang, Haixiao Wang, Yeliu Liu
Summary: The study revealed that RNF144A-AS1 is significantly upregulated in gastric cancer tissues and promotes tumor metastasis, angiogenesis, and proliferation by regulating the miR-30c-2-3p/LOX axis. This highlights the importance of the RNF144A-AS1/miR-30c-2-3p/LOX axis in therapeutic interventions for gastric cancer.
CELL AND BIOSCIENCE
(2021)
Review
Medicine, Research & Experimental
Enjun Lv, Jiaqi Sheng, Chengpeng Yu, Dean Rao, Wenjie Huang
Summary: Long noncoding RNAs (lncRNAs) have gained widespread attention as new and crucial molecules involved in biological function regulation, particularly in tumor metastasis such as hepatocellular carcinoma; despite the incomplete understanding of their mechanisms, lncRNAs have been shown to be closely associated with various stages of tumor growth, invasion, and metastasis.
BIOMEDICINE & PHARMACOTHERAPY
(2021)
Article
Engineering, Biomedical
Madison E. Bates, Leia Troop, M. Ethan Brown, Jennifer L. Puetzer
Summary: The application of exogenous LOX protein significantly improves the mechanical performance of tissue engineered constructs, with timing of application being crucial. This study provides insights into the cellular regulation of LOX crosslinking.
ACTA BIOMATERIALIA
(2023)
Article
Integrative & Complementary Medicine
Pei-Sheng Lee, Ying-Ying Lu, Kalyanam Nagabhushanam, Chi-Tang Ho, Hui-Ching Mei, Min-Hsiung Pan
Summary: This study found that Calebin A, a novel compound derived from medicinal turmeric, has a good thermogenesis function and can modulate the composition of gut microbiota, thus reducing obesity induced by high-fat diet. Additionally, Calebin A can decrease body weight, blood sugar levels, and adipose tissue load in obese mice, demonstrating its potential as an anti-obesity agent.
JOURNAL OF TRADITIONAL AND COMPLEMENTARY MEDICINE
(2023)
Article
Chemistry, Applied
Hua-Tsung Lin, An- Yeh, Tuan-Hua David Ho, Min-Hsiung Pan, Shih Hsin Chen
Summary: Cassava starch was modified with amylomaltase (AMase) to investigate its structural changes and retrogradation properties. The AMase treatment resulted in an increase in molecular weight and a reorganization of chain distributions in amylose and amylopectin. The extent of retrogradation was influenced by the enzyme/substrate ratio and reaction time. A new insight into the structural characteristics of the modified starch and its long-term retrogradation was gained through the analysis of the long-to-short chain ratio, the enzyme/substrate ratio, and reaction time.
FOOD HYDROCOLLOIDS
(2023)
Review
Agriculture, Multidisciplinary
Yen-Chun Koh, Chi-Tang Ho, Min-Hsiung Pan
Summary: Mitochondrial dysfunction can lead to cell death, and it has recently been recognized as a strategy for cancer prevention and treatment using chemotherapy drugs or phytochemicals. However, most drugs currently available cannot specifically target cancer cells and may have negative effects on normal cells. Studies have shown that phytochemicals like resveratrol can improve various diseases associated with dysfunctional or damaged mitochondria by regulating mitophagy, reducing oxidative stress, preserving membrane potential, inducing mitochondrial biogenesis, balancing fusion and fission, and enhancing electron transport chain functionality. Limited research suggests that phytochemicals may also protect against cytotoxicity caused by certain cancer drugs that damage mitochondria. Additionally, mitochondrial dysfunction has been linked to COVID-19 and long COVID, and curcumin has shown positive effects on these conditions. Therefore, this review examines the potential benefits of resveratrol and curcumin for cancer treatment/prevention and disease amelioration. It also discusses the potential of phytochemicals as adjuvant treatments for COVID-19 and long COVID by targeting mitochondrial rescue. The review aims to provide new insights into disease treatment using phytochemicals targeting mitochondria.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Agriculture, Multidisciplinary
Yuhong Mao, Wenting Huang, Rongju Jia, Yangyang Bian, Min-Hsiung Pan, Xiuyun Ye
Summary: This study aimed to examine the impact of protein features on the properties of nanoparticles assembled using the pH-shifting approach, and to understand the underlying mechanism. Four legume protein isolates were fractionated into natural aqueous-soluble and aqueous-insoluble fractions, which served as shell and core for the nanoparticles, respectively. Using zein as the core instead of the aqueous-insoluble fractions improved size uniformity, and particle size could be precisely controlled by adjusting core/shell ratios. Proteomic analysis and computational characterization revealed that hydrophobicity, rather than molecular weight or surface charge, predominantly determined particle size. Molecular docking, structural analysis, and dissociation tests showed that the assembly of zein/aqueous-soluble-based nanoparticles was primarily driven by hydrophobic interactions. This study provides valuable insights into the correlation between protein features and the properties of pH-driven-assembled nanoparticles, enabling precise control over particle size.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Agriculture, Multidisciplinary
Chun-Han Chang, You-Tsz Lien, Wei-Sheng Lin, Kalyanam Nagabhushanam, Chi-Tang Ho, Min-Hsiung Pan
Summary: Evidence suggests that dietary intake of polycyclic aromatic hydrocarbons (PAHs) from food processing can lead to the development of colorectal cancer (CRC) by inducing cellular DNA damage. In this study, piceatannol (PIC) was found to effectively inhibit B[a]P-induced cytochrome P450 1B1 (CYP1B1) protein expression in human colon epithelial cells and alleviate DNA damage. PIC also exhibited antioxidative effects by increasing glutathione (GSH) content and scavenging reactive oxygen species (ROS) induced by B[a]P. Furthermore, PIC activated the Nrf2 signaling pathway and upregulated phase II detoxification enzymes and antioxidative enzymes. These findings suggest that PIC has the potential to prevent CRC by protecting against DNA damage and modulating the metabolism and detoxification of B[a]P.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Food Science & Technology
Chiung-Jung Wen, Yen-Chun Koh, Yen-Chen Tung, Pin-Yu Ho, Shu-Chen Hsieh, Yi-Chen Lo, Jaw-Shiun Tsai, Min-Hsiung Pan
Summary: This study aims to explore the relationship between serum metabolites and gut microbiota in non-robust older adults as a potential diagnostic biomarker. The results show significant differences in gut microbial composition between robust and non-robust subjects, particularly with the abundance of Escherichia/Shigella. Moreover, the abundance of Escherichia/Shigella is positively correlated with the levels of discriminant metabolites. These findings indicate the clear interrelation between gut microbiota and serum metabolites in non-robust older adults, suggesting Escherichia/Shigella as a potential biomarker candidate for robustness sub-phenotypic identification.
MOLECULAR NUTRITION & FOOD RESEARCH
(2023)
Article
Food Science & Technology
Yen-Chun Koh, Dickson Chok, Chun-Han Chang, Chia-Cheng Wei, Chia-Tung Wu, Ko-Chun Cheng, Kalyanam Nagabhushanam, Chi-Tang Ho, Min-Hsiung Pan
Summary: This study aims to evaluate the preventive effect of piceatannol on glutamate-induced neurodegeneration via mitochondrial rescue. The results show that piceatannol can prevent glutamate-induced apoptosis, reduce mitochondrial reactive oxygen species accumulation, promote mitochondrial biogenesis, and preserve mitochondrial functionality. Piceatannol effectively protects dopaminergic neurons from degradation and preserves their controlled responses.
MOLECULAR NUTRITION & FOOD RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Donporn Wongwaiwech, Sudthida Kamchonemenukool, Chi-Tang Ho, Shiming Li, Nutthaporn Majai, Tepsuda Rungrat, Kawee Sujipuli, Min-Hsiung Pan, Monthana Weerawatanakorn
Summary: Crude rice bran oils from different rice cultivars and extraction methods have varying levels of nutraceuticals. The health benefits of rice bran oil in lowering cholesterol are due in part to non-nutrient nutraceuticals such as ?-oryzanol, phytosterols, and policosanols. In this study, the nutraceutical composition and antioxidant properties of crude rice bran oils extracted using different technologies were compared. The results showed that subcritical liquefied dimethyl ether extraction produced oils with higher yield and higher levels of phenolic compounds, ?-oryzanol, phytosterols, and policosanols compared to conventional hexane extraction and supercritical CO2 extraction. Overall, the choice of extraction method significantly affected the composition and properties of the crude rice bran oil.
Article
Agriculture, Multidisciplinary
Wei-Sheng Lin, Wan-Chen Cheng, Pin-Yu Ho, Chi-Tang Ho, Min-Hsiung Pan
Summary: The study indicates that NBT and 5-DMNB can reduce B[a]P metabolism and protect against B[a]P-induced DNA damage by modulating the AhR/CYP1s signaling pathway. This protective effect was observed in both cell and mouse models. These findings suggest that NBT and 5-DMNB have potential for chemoprevention of colorectal cancer.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Agriculture, Multidisciplinary
Yu Xiao, Min-hsiung Pan, Yi-shiou Chiou, Zhenshun Li, Shudong Wei, Xiaoli Yin, Baomiao Ding
Summary: The interaction mechanism between nanoliposomes (NL) and soybean protein isolate (SPI) was studied. It was found that NL formed complexes with beta-conglycinin (7S) and glycinin (11S), resulting in static quenching of the endogenous fluorescence emissions of 7S and 11S, and increased polarity of the SPI fluorophore. The interaction between NL and SPI was exothermic, altered the secondary structures of 7S/11S, exposed more hydrophobic groups on protein surfaces, and had a large zeta potential to ensure system stability. Hydrophobic forces, hydrogen bonds, and salt bridge played important roles in the interaction, which were mainly influenced by the protein characteristics such as amino acid composition, surface hydrophobicity, and advanced structure. These findings provide a deeper understanding of the interaction mechanism between NL and SPI.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Agriculture, Multidisciplinary
Chun Ming How, Ko-Chun Cheng, Yong-Shan Li, Min-Hsiung Pan, Chia-Cheng Wei
Summary: This study investigated the effects of chronic exposure to Benzo[a]pyrene (BaP) on aging in Caenorhabditis elegans, and found that BaP exposure aggravated protein aggregation, shortened lifespan, and worsened age-related decline in motility and pharyngeal pumping. In addition, BaP exposure increased the expression of heat shock proteins and reduced survival under heat stress. However, dietary supplementation with Tangeretin (TAN) alleviated the BaP-induced decline in motility, pumping, and protein accumulation, and restored heat shock proteins' expression levels.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Food Science & Technology
Yen-Chun Koh, Shin-Jhih Lin, Kai-Yu Hsu, Kalyanam Nagabhushanam, Chi-Tang Ho, Min-Hsiung Pan
Summary: The study suggests that PSB and PIN, two analogs of resveratrol, can enhance thermogenesis and mitochondrial biogenesis, thus improving obesity and gut microbiota dysbiosis. PSB has a greater effect than PIN in activating the SIRT1 pathway.
MOLECULAR NUTRITION & FOOD RESEARCH
(2023)
Article
Chemistry, Multidisciplinary
Monthana Weerawatanakorn, Sang He, Chun-Han Chang, Yen-Chun Koh, Meei-Ju Yang, Min-Hsiung Pan
Summary: Obesity and overweight pose a growing risk for various health conditions, and diet, especially tea consumption, has been identified as a potential intervention. This study aimed to investigate the effects of GABA oolong tea extracts (OTEs) on high-fat diet (HFD)-induced obesity using male C57BL/6 J mice. The results showed that GABA OTEs reduced leptin expression, protected against fatty liver disease, and promoted thermogenesis and lipid metabolism, therefore preventing obesity development.
Article
Chemistry, Multidisciplinary
Wei-Sheng Lin, Wan-Chen Cheng, Min-Hsiung Pan
Summary: Inflammatory bowel disease (IBD) is a chronic inflammation of the intestines, and Virofree has shown potential in preventing this disease. This study found that Virofree can alleviate intestinal inflammation and modulate the gut microbiota in mice.
Article
Agriculture, Multidisciplinary
Chun Ming How, Ko-Chun Cheng, Yong-Shan Li, Min-Hsiung Pan, Chia-Cheng Wei
Summary: This study finds that chronic exposure to Benzo[a]pyrene (BaP) has negative effects on aging, and Tangeretin (TAN) supplementation can alleviate the aging toxicity induced by BaP.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
