期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 62, 期 1, 页码 107-115出版社
AMER CHEMICAL SOC
DOI: 10.1021/jf4045638
关键词
multiphase; broccoli; corn; wheat; seeds; NMR spectroscopy; intact sample; in situ analysis; triacylglyceride (TAG)
资金
- Natural Sciences and Engineering Research Council of Canada NSERC
- Canada Foundation for Innovation (CFI)
- Ontario Ministry of Research and Innovation (MRI)
- Krembil Foundation
- Government of Ontario
- Dutch Innovation Program Food and Nutrition Delta [FND09002]
Seeds are complex entities composed of liquids, gels, and solids. NMR spectroscopy is a powerful tool for studying molecular structure but has evolved into two fields, solution and solid state. Comprehensive multiphase (CMP) NMR spectroscopy is capable of liquid-, gel-, and solid-state experiments for studying intact samples where all organic components are studied and differentiated in situ. Herein, intact C-13-labeled seeds were studied by a variety of 1D/2D H-1/C-13 experiments. In the mobile phase, an assortment of metabolites in a single C-13-labeled wheat seed were identified; the gel phase was dominated by triacylglycerides; the semisolid phase was composed largely of carbohydrate biopolymers, and the solid phase was greatly influenced by starchy endosperm signals. Subsequently, the seeds were compared and relative similarities and differences between seed types discussed. This study represents the first application of CMP-NMR to food chemistry and demonstrates its general utility and feasibility for studying intact heterogeneous samples.
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