期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 61, 期 23, 页码 5590-5599出版社
AMER CHEMICAL SOC
DOI: 10.1021/jf3035989
关键词
nanosized titanium dioxide; spleen damage; cyclooxygenase-2; cyclic adenosine monophosphate-response element; MAPKs/PI3-K/Akt signaling pathways
资金
- National Natural Science Foundation of China [81273036, 30901218, 30972504, 81172697]
- Priority Academic Program Development of Jiangsu Higher Education Institutions
- Major State Basic Research Development Program of China (973 Program) [2006CB705602]
- National Important Project on Scientific Research of China [2011CB933404]
- National New Ideas Foundation of Student of China [111028534]
- Chun-Tsung scholar Foundation of Soochow University
Due to an increase in surface area per particle weight, nanosized titanium dioxide (nano-TiO2) has greatly increased its function as a catalyst and is used for whitening and brightening foods. However, concerns over the safety of nano-TiO2 have been raised. The purpose of this study was to determine whether the protein kinase MAPKs/PI3-K/Akt signaling pathways and transcription factors are activated prior to or concurrent with COX-2 up-regulation in mouse spleen following exposure to 10 mg/kg BW of pure anatase nano-TiO2 by the intragastric route for 15-90 days. The study clearly showed that nano-TiO2 was deposited in the spleen and resulted in reactive oxygen species production, time dependent splenic inflammation, and necrosis, coupled with a 12.64-64.06% increase in COX-2 and prostaglandin E2 expression, respectively. Furthermore, nano-TiO2 elevated the expressions of ERK, AP-1, CRE, Akt, JNK2, MAPKs, PI3-K, c-Jun, and c-Fos in the spleen by 1.08-6-fold with increased exposure duration, respectively. These findings suggested that nano-TiO2-induced COX-2 expression may be mediated predominantly through the induction of AP-1 and CRE and that AP-1/CRE induction occurred via the MAPKs/PI3-K/Akt signaling pathways in the spleen. Therefore, the findings suggest the need for caution when using nanomaterials as food additives.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据