Article
Green & Sustainable Science & Technology
Hongyi Pan, Ping Kou, Jie Yang, Lili Niu, Ning Wan, Chunjian Zhao, Zhiguo Liu, Chengbo Gu, Yujie Fu
Summary: A novel method for synchronous extraction and enrichment of hydrophilic and hydrophobic components from pigeon pea leaves was developed, achieving higher extraction yields and target compound contents. The SHS used in the extraction process demonstrated stable extraction yields for at least 5 cycles and showed good biocompatibility with the target compounds. This method is an environmentally friendly, promising, and sustainable alternative for extracting and enriching phytochemicals from plant materials simultaneously.
JOURNAL OF CLEANER PRODUCTION
(2021)
Article
Agriculture, Multidisciplinary
Qing-Yan Gai, Jin-Xian Fu, Yao Lu, Lan Yao, Run-Ze Cao, Xiao-Jia He, Xue Feng, Yu-Jie Fu, Jiao Jiao
Summary: This study used UV-B treatment to promote the accumulation of health-promoting phenolic compounds in pigeon pea seeds, leading to increased antioxidant activities. UV-B radiation also caused tissue damages and enhanced the generation of endogenous salicylic acid, activating the expression of related genes. This research reveals the importance of pigeon pea seeds as functional foods/nutraceuticals in the diet.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Chemistry, Analytical
Zili Guo, Peixi Zhu, Xiaoai He, Tianhe Yan, Xianrui Liang
Summary: A method based on ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry using electrospray ionization was established to identify chemical constituents in pigeon pea leaves ethanol extract. A total of 58 compounds were detected, with 42 compounds unambiguously identified or tentatively assigned. Eight sets of isomers were differentiated by ion trap mass spectrometry based on fragment ion differences, and energy-resolved mass spectrometry was adopted in the study for relative abundance analysis.
JOURNAL OF SEPARATION SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Aderonke Olagunju, Adeola M. Alashi, Olufunmilayo S. Omoba, Victor N. Enijiugha, Rotimi E. Aluko
Summary: In this study, pigeon pea protein was digested and separated into fractions using chromatography. The active fractions exhibited antioxidant properties and inhibited renin and ACE. The most active peptide sequences, such as AGVTVS, showed potential for industrial utilization and therapeutic applications.
JOURNAL OF FOOD BIOCHEMISTRY
(2022)
Article
Food Science & Technology
Shubhra Shekhar, Prem Prakash, Poonam Singha, Kamlesh Prasad, Sushil Kumar Singh
Summary: This study investigates the effect of ultrasound on the extraction of bioactive compounds from garlic leaf powder. The experiment explores the optimal conditions for maximum extraction yield, total phenol content, total flavonoid content, and antioxidant activity. The results suggest that ultrasound-assisted extraction at 53% amplitude, 13 minutes treatment time, and 50% ethanol concentration provides the highest extraction yield and bioactive compound content.
Article
Biochemistry & Molecular Biology
Khizar Hayat, Asif Khan, Farkhanda Bibi, Salahuddin, Waheed Murad, Yujie Fu, Gaber El-Saber Batiha, Mohammed Alqarni, Ajmal Khan, Ahmed Al-Harrasi
Summary: The study found that exposure to soil contaminated with cadmium and copper significantly inhibited the growth and photosynthetic activity of pigeon pea plants, leading to decreased nutrient content. High metal concentrations induced oxidative stress, increased antioxidant enzyme activities, and reduced biosynthesis of secondary metabolites, which could result in a decline in the medicinal properties of pigeon pea.
Article
Biochemistry & Molecular Biology
Duangjai Tungmunnithum, Samantha Drouet, Jose Manuel Lorenzo, Christophe Hano
Summary: Pigeon pea seeds are a rich source of antioxidants and anti-inflammatory flavonoids, especially isoflavones. The ultrasound-assisted extraction (USAE) method was optimized and compared with conventional methods, showing higher extraction efficiency and preservation of antioxidant activities. The extract obtained under optimal USAE conditions demonstrated significant antioxidant and anti-aging potentials at a cellular level.
Article
Chemistry, Applied
Nushrat Yeasmen, Valeria Orsat
Summary: Concentrations and attributes of antioxidant components in sugar maple leaves were studied based on HPLC-UV and radical scavenging/redox potential methods respectively. Colorimetry, SEM, and FTIR spectroscopy measurements were conducted to characterize the composition of leaves throughout the growth cycle. The results showed that the antioxidant activities of sugar maple leaves were strongly associated with phenolic contents and varied significantly with harvesting time. The FTIR spectra revealed the presence of functional groups that are important for the antioxidant activity. Principal component analysis indicated that the biosynthesis of phenolic compounds in sugar maple leaves mainly occurs during the leaf expansion and growth phases. These findings provide valuable insight into the effect of harvesting time on the phenolic composition of sugar maple leaves and their potential use as functional food ingredients.
Article
Plant Sciences
Zlata V. Ogneva, Vlada V. Volkonskaia, Alexandra S. Dubrovina, Andrey R. Suprun, Olga A. Aleynova, Konstantin V. Kiselev
Summary: The study found that the application of trans-resveratrol and trans-piceid can enhance Arabidopsis thaliana plants' tolerance to UV-B, increasing leaf survival rates. This improvement in tolerance is more effective than using precursors but slightly less effective than using a UV-B absorber. Furthermore, trans-resveratrol also increased the expression of antioxidant and stress-inducible genes.
Article
Agricultural Engineering
Zi-Hui Cai, Lin Liu, Qi Gu, Jian-Dong Wang, Guo-Sheng Liu, Quan Zhou, Xin-Yue Dong, Su Zhang, Yu-Jie Fu
Summary: In this study, a novel green natural deep eutectic solvent-based supramolecular solvent/equilibrium solution (NADES-SUPRAS/EqS) system was designed and characterized. It was used for efficient extraction and enrichment of active ingredients in Cajanus cajan leaves. Various influencing factors were adjusted to achieve optimal extraction parameters and high extraction rates with excellent enrichment effect in a short time. An integrated multi-stage counter-current extraction and recovery unit was developed to reduce costs and facilitate large-scale applications in the industry.
INDUSTRIAL CROPS AND PRODUCTS
(2023)
Article
Engineering, Chemical
Gan-Lu Yue, Yuan-Hang Chang, Lian Yu, Jian Xu, Ning Wan, Cong-Jia Duan, Ying Zhang, Yu-Jie Fu
Summary: In this study, a V-type deep eutectic solvent composed of thymol/coumarin was designed and used for the extraction of stilbene structure compounds from pigeon pea leaves. The optimal extraction conditions were determined, and the addition of ammonia solution was found to effectively disrupt the hydrogen bonding structure of the solvent. Recycled target components and separated solvent were achieved through macroporous resin adsorption. Physicochemical properties and extraction mechanisms were investigated using FT-IR, UV-vis, and NMR. This study provides a new solvent screening idea and a green extraction and separation process for natural products.
CHEMICAL ENGINEERING RESEARCH & DESIGN
(2023)
Review
Chemistry, Applied
Na Guo, Xiao-Qiao Zhai, Guo-Qiang Fan
Summary: The flowers of Paulownia plants have been used traditionally in medicinal products and are gaining attention for their wood production. These flowers contain flavonoids, phenylpropanoids, terpenoids, volatile components, polysaccharides, lignans, and iridoids, which have various health benefits and promote the development of new products and technologies. The review provides information on the chemical composition, biological and pharmacological activities, and economic values of Paulownia flowers, serving as a reference for their further utilization.
Article
Chemistry, Applied
Peter A. Segun, Omonike O. Ogbole, Toluwanimi E. Akinleye, Temitope O. C. Faleye, Adekunle J. Adeniji
Summary: Three stilbenoids isolated from Macaranga barteri leaves show anti-enteroviral activity, especially vedelianin (2) exhibiting the best selectivity on echovirus E19. These compounds may be responsible for the traditional use of Macaranga barteri in treating viral infections.
NATURAL PRODUCT RESEARCH
(2021)
Article
Agriculture, Dairy & Animal Science
Tefera Mekonen, Adugna Tolera, Ajebu Nurfeta, Barry Bradford, Sintayehu Yigrem, Jessie Vipham
Summary: Protein supplements are expensive and not easily accessible in small-scale livestock production systems. This study investigated the inclusion of pigeon pea leaves in the concentrate mixture for dairy cows. The results showed that including up to 30% of pigeon pea leaves in the concentrate mixture had no significant effect on feed intake, milk yield and composition, and blood metabolites of crossbred dairy cows. Further research is needed to evaluate the effect of higher levels of pigeon pea leaves in the concentrate mixture on lactating dairy cows.
Article
Biochemistry & Molecular Biology
Aderonke I. Olagunju, Olufunmilayo S. Omoba, Victor N. Enujiugha, Adeola M. Alashi, Rotimi E. Aluko
Summary: Enzymatic hydrolysis of pigeon pea protein using thermoase produced peptide fractions with antioxidant, antihypertensive, and antidiabetic properties. The fractionated peptides showed stronger DPPH scavenging, renin inhibition, and inhibition of alpha-amylase and alpha-glucosidase activities compared to the crude hydrolysate. These findings suggest that the peptide fractions could have potential applications in formulating functional foods for hypertension and diabetes management.
JOURNAL OF FOOD BIOCHEMISTRY
(2021)
Article
Biology
Onat Kadioglu, Faranak Bahramimehr, Mona Dawood, Nuha Mahmoud, Mohamed Elbadawi, Xiaohua Lu, Yagmur Buelbuel, Jana Agnieszka Schulz, Lisa Kraemer, Marie-Kathrin Urschel, Zoe Kuenzli, Leila Abdulrahman, Fadwa Aboumaachar, Lajien Kadalo, Le Van Nguyen, Sara Shaidaei, Nawal Thaher, Kathrin Walter, Karolin Christiane Besler, Andreas Spuller, Markus Munder, Henry Johannes Greten, Thomas Efferth
Summary: RNA-sequencing is utilized to develop individualized therapy concepts for cancer patients based on their tumor-specific mutational profiles. In this study, drugs and inhibitors were identified through virtual drug screening of FDA-approved drugs and mining the Drug Gene Interaction (DGI) database for missense mutated proteins in 35 tumors. The approach offers new treatment options for patients with refractory tumors that do not respond to standard chemotherapy.
COMPUTERS IN BIOLOGY AND MEDICINE
(2023)
Article
Medicine, Research & Experimental
Jasmin Taubenschmid-Stowers, Michael Orthofer, Anna Laemmerer, Christian Krauditsch, Marianna Rozsova, Christian Studer, Daniela Loetsch, Johannes Gojo, Lisa Gabler, Matheus Dyczynski, Thomas Efferth, Astrid Hagelkruys, Georg Widhalm, Andreas Peyrl, Sabine Spiegl-Kreinecker, Dominic Hoepfner, Shan Bian, Walter Berger, Juergen A. Knoblich, Ulrich Elling, Moritz Horn, Josef M. Penninger
Summary: The natural compound Artemisinin is widely used as an antimalarial drug and has potential for anticancer therapy. Through screening with yeast and haploid stem cells, it has been discovered that the porphyrin biosynthesis pathway is crucial for the cytotoxicity of Artemisinin. In various human brain tumor models, a combination treatment of Artemisinin and the porphyrin enhancer 5-aminolevulinic acid sensitizes and kills brain tumor cells.
EMBO MOLECULAR MEDICINE
(2023)
Article
Chemistry, Applied
Inas Y. Younis, Rana M. Ibrahim, Ali. M. El-Halawany, Mohamed-Elamir F. Hegazy, Thomas Efferth, Engy Mohsen
Summary: In this study, LC-QTOF-MS and multivariate statistical tools were used to analyze the composition differences of dragon fruit peel and pulp from Egypt, Germany, Philippines, and China. It was found that dragon fruits from China and Egypt were the most distinct due to their high betalains content. Moreover, different extracts from dragon fruit showed different activities as inhibitors of alpha-glucosidase and AGE formation, as well as antioxidants.
Article
Biochemistry & Molecular Biology
Matteo Rosellini, Petri Turunen, Thomas Efferth
Summary: Plastic in oceans degrades into microplastics and nanoplastics, posing various problems for marine organisms. Recent findings show that microplastics can be found in human blood, breast milk, and placenta, suggesting their ability to enter the human body with unknown effects. Additionally, plastic contains compounds with potential impact on human health, such as plasticizers and antioxidants. This study investigates the interaction between plastic-related compounds and two cellular transporters involved in detoxification of xenobiotics. The results provide evidence that certain plastic compounds can inhibit these transporters and increase their toxic potential.
Article
Biochemistry & Molecular Biology
Luay. J. J. Rashan, Nadire Oezenver, Joelle. C. C. Boulos, Mona Dawood, Wynand. P. P. Roos, Katrin Franke, Ioannis Papasotiriou, Ludger. A. A. Wessjohann, Heinz-Herbert Fiebig, Thomas Efferth
Summary: A study found that a cold-water leaf extract called Breastin from Nerium oleander L. has potential as an anticancer agent, inhibiting the growth of multiple cancer cell lines and bypassing some traditional drug resistance mechanisms. The study also showed that protein expression profiles can be used to predict the sensitivity of tumor cells to Breastin constituents.
Article
Pharmacology & Pharmacy
Mohamed Elbadawi, Joelle C. Boulos, Mona Dawood, Min Zhou, Waseem Gul, Mahmoud A. ElSohly, Sabine M. Klauck, Thomas Efferth
Summary: The proto-oncogenic transcription factor c-MYC is a key player in tumorigenesis, cellular proliferation, and cell death control. This study aimed to investigate the anticancer activity and molecular mechanisms of the artemisinin dimer isoniazide in drug-sensitive and multidrug-resistant leukemia cells. The compound showed potent growth inhibitory activity, good binding with c-MYC, downregulated c-MYC expression, and induced DNA damage, apoptosis, and autophagy.
Editorial Material
Nutrition & Dietetics
Haroon Khan, Thomas Efferth, Silvia Tejada
FRONTIERS IN NUTRITION
(2023)
Review
Chemistry, Analytical
Jiahui Gong, Chunyu Liang, Zahid Majeed, Mengfei Tian, Chunjian Zhao, Meng Luo, Chunying Li
Summary: In this review, the research from 2013 to 2022 regarding the character of ionic liquids, categories of phytochemicals, and the use of imidazolium ionic liquids for phytochemical extraction is presented. The structural formulae and preparation methods of commonly used imidazolium ionic liquids are introduced, along with their application in extracting phytochemicals from plants. The extraction mechanisms, recovery methods, and toxicity of imidazolium ionic liquids are discussed, followed by a summary of the challenges and prospects of using them for phytochemical extraction.
Review
Chemistry, Analytical
Yu Zhang, Mengfei Tian, Zahid Majeed, Yuxin Xie, Kaili Zheng, Zidan Luo, Chunying Li, Chunjian Zhao
Summary: This work summarizes the construction and synthesis of stable hydrogen-bonded organic frameworks (HOFs) and their applications. HOFs are self-assembled from organic structures through hydrogen bonding, non-covalent pi-pi packing, and van der Waals force interaction. They possess high specific surface area, ordered pore structure, adjustable pores, and the ability to adjust various physical and chemical forms. HOF-based materials find applications in adsorption and separation, heavy metal and radioactive metal adsorption, organic dye and pesticide adsorption, energy conversion, organic dye degradation, and pollutant sensing.
Article
Integrative & Complementary Medicine
Laetitia M. Youmbi, Yves S. D. Makong, Armelle T. Mbaveng, Simplice B. Tankeo, Ghislain W. Fotso, Bruno L. Ndjakou, Jean D. Wansi, Veronique P. Beng, Norbert Sewald, Bonaventure T. Ngadjui, Thomas Efferth, Victor Kuete
Summary: This study aimed to identify the cytotoxic constituents of Brucea antidysenterica and demonstrate their mode of induction of apoptosis on cancer cell lines. The results showed that Brucea antidysenterica and its constituents have potential antiproliferative effects and can induce apoptosis through caspase activation, alteration of mitochondrial membrane potential, and increased reactive oxygen species levels.
BMC COMPLEMENTARY MEDICINE AND THERAPIES
(2023)
Article
Biochemistry & Molecular Biology
Julia Schaefer, Vincent Julius Kloesgen, Ejlal A. Omer, Onat Kadioglu, Armelle T. Mbaveng, Victor Kuete, Andreas Hildebrandt, Thomas Efferth
Summary: Cancer therapy is often hindered by drug resistance and severe side effects. Phytochemicals are a valuable resource for developing less toxic drugs. Bioinformatics can simplify the drug development process. This study analyzed 375 phytochemicals and identified six compounds with potential anticancer activity and P-gp inhibition.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Anand Kar, Durgesh Mahar, Sagarika Biswas, Debolina Chakraborty, Thomas Efferth, Sunanda Panda
Summary: This study aimed to evaluate the effect of Ficus religiosa leaf extract in ameliorating hypothyroidism in rats and identify the major bioactive compounds responsible for this activity. The results showed that FL extract could improve hypothyroidism, and the major compounds betulinic acid, chlorogenic acid, and quinic acid may increase thyroid hormone levels by interacting with thyroid hormone receptor and TSH receptor through molecular docking analysis.
JOURNAL OF ETHNOPHARMACOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Assia I. Drif, Bharathi Avula, Ikhlas A. Khan, Thomas Efferth
Summary: This study investigated the inhibitory activities of secondary metabolites from chamomile against COX2, an enzyme involved in inflammation and cancer development. It also evaluated the cytotoxicity of these compounds on cancer cell lines. Myricetin, a compound found in chamomile tea, was further studied to determine its effects on tumor cells and its potential as a therapeutic agent. Overall, this study provides insight into the molecular mechanisms of chamomile secondary metabolites and their potential in cancer prevention or treatment.
APPLIED SCIENCES-BASEL
(2023)
Article
Oncology
Joelle C. Boulos, Manik Chatterjee, Letian Shan, Thomas Efferth
Summary: Hematological neoplasms are difficult to cure, and new treatment approaches targeting c-MYC are needed. In this study, adapalene, an FDA-approved drug, was identified as a potential c-MYC inhibitor, providing hope for the development of new anticancer medications. Adapalene showed cytotoxicity against multiple myeloma cells and decreased c-MYC expression and transcriptional activity, resulting in cell cycle arrest, DNA damage, and induction of apoptosis and autophagy.
Article
Integrative & Complementary Medicine
Valaire Y. Matieta, Armelle T. Mbaveng, Guy R. Sado Nouemsi, Simplice B. Tankeo, Gabriel T. Kamsu, Paul Nayim, Alain M. Lannang, Ilhami Celik, Thomas Efferth, Victor Kuete
Summary: This study found that Bauhinia thonningii and its constituents have promising cytotoxic effects on human cancer cell lines, without causing toxicity in rats.
BMC COMPLEMENTARY MEDICINE AND THERAPIES
(2023)