Article
Engineering, Environmental
Yaqiang Cao, Yuanyuan Chen, Ke Miao, Shuyi Zhang, Fuchang Deng, Mu Zhu, Chao Wang, Wen Gu, Yixuan Huang, Zijin Shao, Xiaoyan Dong, Yufeng Gong, Hui Peng, Hui Yang, Yi Wan, Xudong Jia, Song Tang
Summary: Mounting evidence shows the close association between ambient PM2.5 exposure and obesity development, with adipose tissue being an important target. This study employed a preadipocyte differentiation model to comprehensively explore the adipogenic potential of PM2.5. PM2.5 exposure significantly increased fatty acid uptake, lipid accumulation, and adipogenic differentiation in a concentration-dependent manner. Transcriptome and lipidome analyses revealed disruption of transcriptional and lipid profiling at 10 μg/mL PM2.5. Activation of the peroxisome proliferator-activated receptor (PPAR) pathway and metabolism of glycerophospholipids, glycerolipids, and sphingolipids were most affected during adipocyte differentiation. The study demonstrated that PM2.5 promoted adipogenesis by activating PPAR. through reporter gene assays. The findings provide insights into PM2.5-induced adipogenesis and potential biomolecular targets for preventing PM2.5-induced obesity and related metabolic diseases.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Yuankuan Li, Sung Ho Lee, Meiyu Piao, Hyung Sik Kim, Kwang Youl Lee
Summary: This study found that Metallothionein 3 (MT3) plays an important role in the differentiation of 3T3-L1 cells into adipocytes. Overexpression of MT3 inhibited adipocyte differentiation and reduced the levels of adipogenic transcription factors C/EBPa and PPAR?. Further analysis revealed that MT3 suppressed the transcriptional activity of PPAR?, and this effect was not mediated by a direct interaction between MT3 and PPAR?. Additionally, MT3 overexpression decreased reactive oxygen species (ROS) levels during early adipocyte differentiation, while treatment with antimycin A, which induces ROS generation, restored the ROS levels.
Article
Pharmacology & Pharmacy
Chaiwat Monmai, Jin-Suk Kim, Hyun Bo Sim, Doh-Won Yun, Sung-Dug Oh, Eui-Shik Rha, Jong-Jin Kim, So-Hyeon Baek
Summary: This study evaluated the effects of extracts from transgenic rice on inhibiting adipogenesis and anti-inflammatory activity. The results showed that the extracts significantly reduced lipid accumulation in cells and inhibited the expression of adipogenesis-related mRNA and inflammation-related factors. Therefore, transgenic rice may have potential as an obesity-reducing and/or -preventing agent.
Article
Biology
Catarina Teixeira, Andre P. Sousa, Ines Santos, Ana Catarina Rocha, Ines Alencastre, Ana Claudia Pereira, Daniela Martins-Mendes, Pedro Barata, Pilar Baylina, Ruben Fernandes
Summary: This study demonstrates that pioglitazone enhances adipocyte differentiation and secretome production, making it an excellent model for studying adiposity. The study also proposes an improved method for adipocyte secretome production, which can be used for experimental biology studies to understand the molecular mechanisms underlying the influence of adiposity on other cells.
Article
Nutrition & Dietetics
Elisabetta Melloni, Silvia Grassilli, Arianna Romani, Erika Rimondi, Annalisa Marcuzzi, Enrico Zauli, Paola Secchiero, Guglielmo Paganetto, Alessandra Guerrini, Gianni Sacchetti, Massimo Tacchini
Summary: Convolvulus pluricaulis is a commonly used Indian herb in Ayurvedic medicine known for its neuroprotective and neuroinflammatory action. Recent studies have shown its involvement in lipidic and glucidic metabolism, particularly in the control of hyperlipidaemia and diabetic conditions. It has been found to promote adipocyte differentiation and regulate glucose metabolism through the modulation of key regulators such as PPAR gamma and GLUT-4. Additionally, it exhibits anti-inflammatory effects by downregulating cytokines associated with insulin resistance and glucose intolerance. These findings suggest that Convolvulus pluricaulis could have potential benefits in glycemia and may be used as an adjunctive treatment for diabetes or for better glucose control.
Article
Cell Biology
Xin Lv, Jun Qiu, Tao Hao, Haoran Zhang, Haiping Jiang, Yang Tan
Summary: The study demonstrated that TSA inhibited adipogenesis in 3T3-L1 preadipocytes by activating the AMPK pathway, leading to decreased lipid accumulation. Furthermore, TSA was also able to reverse the weight gain and adipose tissue increase induced by a high-fat diet in obese mouse models.
Article
Integrative & Complementary Medicine
Eunbi Koh, Boram Kim, Kyungoh Choi
Summary: The study found that Torreya nucifera seed oil (TNSO) can promote adipocyte differentiation, increase intracellular lipid accumulation, and improve adipocyte function by activating adipogenic gene expression and PPAR gamma, STAT3 pathways.
BMC COMPLEMENTARY MEDICINE AND THERAPIES
(2021)
Article
Multidisciplinary Sciences
Dandan Zhao, Yanyun Pan, Na Yu, Ying Bai, Rufeng Ma, Fangfang Mo, Jiacheng Zuo, Beibei Chen, Qiangqiang Jia, Dongwei Zhang, Jiaxian Liu, Guanjian Jiang, Sihua Gao
Summary: Accumulating evidence suggests that curcumin promotes the adipogenic differentiation of preadipocytes and enhances mitochondrial oxygen consumption in 3T3-L1 mature adipocytes by regulating the expression of UCP1, PRDM16, PPAR gamma, and PGC-1 alpha.
ROYAL SOCIETY OPEN SCIENCE
(2021)
Article
Pharmacology & Pharmacy
Junjie Yue, Caiting Sun, Jinyuan Tang, Qiyuan Zhang, Mengjie Lou, Hongwen Sun, Lianying Zhang
Summary: 2-Ethylhexyl diphenyl phosphate (EHDPP), a commonly used flame retardant and food packaging material, exhibits high lipophilicity and bioaccumulation properties, primarily targeting adipocytes. The toxicity of EHDPP on preadipocytes and the underlying mechanism have not been fully understood. This study identified 7 differentially expressed miRNAs in 3T3-L1 preadipocytes exposed to EHDPP, with miR-155-5p being the most significantly down-regulated miRNA. Inhibition of miR-155-5p promoted adipocyte differentiation, while its overexpression counteracted EHDPP-induced adipogenesis.
Article
Biochemistry & Molecular Biology
Hyo-Shin Kwon, Gil-Saeng Jeong, Byeong-Churl Jang
Summary: CTXA from Cudrania tricuspidata Bureau exhibits strong anti-adipogenic and anti-inflammatory effects on 3T3-L1 cells by regulating the expression and phosphorylation levels of C/EBP-alpha, PPAR-gamma, FAS, ACC, perilipin A, STAT-3/5, AMPK, and iNOS.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Vaikundamoorthy Ramalingam, Inho Hwang
Summary: The study demonstrated that ZVZ nanoparticles can effectively induce adipogenesis and increase lipid accumulation in 3T3-L1 cells, while regulating cell differentiation by upregulating the expression of key genes.
PROCESS BIOCHEMISTRY
(2021)
Article
Chemistry, Medicinal
Jian Gao, Fan Li, Ye Huang, Shihao Li, Qisi Lin
Summary: The naphthofuran derivative BF4, a SIRT1 activator, was found to regulate lipid metabolism in 3T3-L1 adipocytes by inhibiting adipogenesis and lipid accumulation. This effect was mediated through the activation of the SIRT1/AMPK pathway and downregulation of key regulators in adipocyte differentiation.
MEDICINAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Eun-Ok Lee, Hee-Kyoung Joo, Yu-Ran Lee, Sungmin Kim, Kwon-Ho Lee, Sang-Do Lee, Byeong-Hwa Jeon
Summary: Apurinic/apyrimidinic endonuclease 1/redox factor-1 (APE1/Ref-1) is involved in DNA repair and redox regulation. It inhibits adipocyte differentiation by regulating adipogenic transcription factors, suggesting it as a potential therapeutic target for regulating adipocyte differentiation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Anil Kumar Yadav, Byeong-Churl Jang
Summary: The study found that pazopanib significantly reduces lipid accumulation and triglyceride content during the differentiation of 3T3-L1 preadipocytes, while also inhibiting the occurrence of inflammation. This process is accompanied by the regulation of multiple key signaling pathways.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Sung Ho Ahn, Hyun Jung Lee, Do Hyeon Pyun, Tae Jin Kim, A. M. Abd El-Aty, Jin-Ho Song, Yong Kyoo Shin, Ji Hoon Jeong, Eon Sub Park, Tae Woo Jung
Summary: CAP can attenuate lipogenesis and enhance lipolysis in adipocytes through the irisin/AMPK pathway, suggesting that it may be a novel therapeutic agent for obesity.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Integrative & Complementary Medicine
Pei-Sheng Lee, Ying-Ying Lu, Kalyanam Nagabhushanam, Chi-Tang Ho, Hui-Ching Mei, Min-Hsiung Pan
Summary: This study found that Calebin A, a novel compound derived from medicinal turmeric, has a good thermogenesis function and can modulate the composition of gut microbiota, thus reducing obesity induced by high-fat diet. Additionally, Calebin A can decrease body weight, blood sugar levels, and adipose tissue load in obese mice, demonstrating its potential as an anti-obesity agent.
JOURNAL OF TRADITIONAL AND COMPLEMENTARY MEDICINE
(2023)
Article
Chemistry, Applied
Hua-Tsung Lin, An- Yeh, Tuan-Hua David Ho, Min-Hsiung Pan, Shih Hsin Chen
Summary: Cassava starch was modified with amylomaltase (AMase) to investigate its structural changes and retrogradation properties. The AMase treatment resulted in an increase in molecular weight and a reorganization of chain distributions in amylose and amylopectin. The extent of retrogradation was influenced by the enzyme/substrate ratio and reaction time. A new insight into the structural characteristics of the modified starch and its long-term retrogradation was gained through the analysis of the long-to-short chain ratio, the enzyme/substrate ratio, and reaction time.
FOOD HYDROCOLLOIDS
(2023)
Article
Agriculture, Multidisciplinary
Wei-Sheng Lin, Tai-Ling Chueh, Kalyanam Nagabhushanam, Chi -Tang Ho, Min-Hsiung Pan
Summary: This study evaluated the effects of Piceatannol (PIC) and 3'-hydroxypterostilbene (HPSB) on DSS-induced colitis in mice. The findings showed that both PIC and HPSB attenuated inflammation by inhibiting specific pathways and reducing inflammasome activation. PIC was also effective in modulating tight junctions. Additionally, both PIC and HPSB altered the composition of gut microbiota. These results suggest that PIC may be more effective in maintaining gut barrier integrity than HPSB and could be a promising ingredient for colitis prevention.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Food Science & Technology
Yen-Chun Koh, Lee-Han Kuo, Ya-Yun Chang, Yen-Chen Tung, Yi-Chen Lo, Min-Hsiung Pan
Summary: This study investigates the anti-aging potential of fermented black soybean and adlay (FBA) supplements. The results show that FBA supplementation prevents muscle loss, reduces aging biomarkers, improves gut microbial dysbiosis, and reverses the reduced ability of microbiota in aged mice. These findings suggest that FBA has significant anti-aging effects and can be developed as a functional food for healthy aging.
MOLECULAR NUTRITION & FOOD RESEARCH
(2023)
Review
Agriculture, Multidisciplinary
Yen-Chun Koh, Chi-Tang Ho, Min-Hsiung Pan
Summary: Mitochondrial dysfunction can lead to cell death, and it has recently been recognized as a strategy for cancer prevention and treatment using chemotherapy drugs or phytochemicals. However, most drugs currently available cannot specifically target cancer cells and may have negative effects on normal cells. Studies have shown that phytochemicals like resveratrol can improve various diseases associated with dysfunctional or damaged mitochondria by regulating mitophagy, reducing oxidative stress, preserving membrane potential, inducing mitochondrial biogenesis, balancing fusion and fission, and enhancing electron transport chain functionality. Limited research suggests that phytochemicals may also protect against cytotoxicity caused by certain cancer drugs that damage mitochondria. Additionally, mitochondrial dysfunction has been linked to COVID-19 and long COVID, and curcumin has shown positive effects on these conditions. Therefore, this review examines the potential benefits of resveratrol and curcumin for cancer treatment/prevention and disease amelioration. It also discusses the potential of phytochemicals as adjuvant treatments for COVID-19 and long COVID by targeting mitochondrial rescue. The review aims to provide new insights into disease treatment using phytochemicals targeting mitochondria.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Agriculture, Multidisciplinary
Yuhong Mao, Wenting Huang, Rongju Jia, Yangyang Bian, Min-Hsiung Pan, Xiuyun Ye
Summary: This study aimed to examine the impact of protein features on the properties of nanoparticles assembled using the pH-shifting approach, and to understand the underlying mechanism. Four legume protein isolates were fractionated into natural aqueous-soluble and aqueous-insoluble fractions, which served as shell and core for the nanoparticles, respectively. Using zein as the core instead of the aqueous-insoluble fractions improved size uniformity, and particle size could be precisely controlled by adjusting core/shell ratios. Proteomic analysis and computational characterization revealed that hydrophobicity, rather than molecular weight or surface charge, predominantly determined particle size. Molecular docking, structural analysis, and dissociation tests showed that the assembly of zein/aqueous-soluble-based nanoparticles was primarily driven by hydrophobic interactions. This study provides valuable insights into the correlation between protein features and the properties of pH-driven-assembled nanoparticles, enabling precise control over particle size.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Agriculture, Multidisciplinary
Chun-Han Chang, You-Tsz Lien, Wei-Sheng Lin, Kalyanam Nagabhushanam, Chi-Tang Ho, Min-Hsiung Pan
Summary: Evidence suggests that dietary intake of polycyclic aromatic hydrocarbons (PAHs) from food processing can lead to the development of colorectal cancer (CRC) by inducing cellular DNA damage. In this study, piceatannol (PIC) was found to effectively inhibit B[a]P-induced cytochrome P450 1B1 (CYP1B1) protein expression in human colon epithelial cells and alleviate DNA damage. PIC also exhibited antioxidative effects by increasing glutathione (GSH) content and scavenging reactive oxygen species (ROS) induced by B[a]P. Furthermore, PIC activated the Nrf2 signaling pathway and upregulated phase II detoxification enzymes and antioxidative enzymes. These findings suggest that PIC has the potential to prevent CRC by protecting against DNA damage and modulating the metabolism and detoxification of B[a]P.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Agriculture, Multidisciplinary
Wei-Sheng Lin, Wan-Chen Cheng, Pin-Yu Ho, Chi-Tang Ho, Min-Hsiung Pan
Summary: The study indicates that NBT and 5-DMNB can reduce B[a]P metabolism and protect against B[a]P-induced DNA damage by modulating the AhR/CYP1s signaling pathway. This protective effect was observed in both cell and mouse models. These findings suggest that NBT and 5-DMNB have potential for chemoprevention of colorectal cancer.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Agriculture, Multidisciplinary
Yu Xiao, Min-hsiung Pan, Yi-shiou Chiou, Zhenshun Li, Shudong Wei, Xiaoli Yin, Baomiao Ding
Summary: The interaction mechanism between nanoliposomes (NL) and soybean protein isolate (SPI) was studied. It was found that NL formed complexes with beta-conglycinin (7S) and glycinin (11S), resulting in static quenching of the endogenous fluorescence emissions of 7S and 11S, and increased polarity of the SPI fluorophore. The interaction between NL and SPI was exothermic, altered the secondary structures of 7S/11S, exposed more hydrophobic groups on protein surfaces, and had a large zeta potential to ensure system stability. Hydrophobic forces, hydrogen bonds, and salt bridge played important roles in the interaction, which were mainly influenced by the protein characteristics such as amino acid composition, surface hydrophobicity, and advanced structure. These findings provide a deeper understanding of the interaction mechanism between NL and SPI.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Agriculture, Multidisciplinary
Chun Ming How, Ko-Chun Cheng, Yong-Shan Li, Min-Hsiung Pan, Chia-Cheng Wei
Summary: This study investigated the effects of chronic exposure to Benzo[a]pyrene (BaP) on aging in Caenorhabditis elegans, and found that BaP exposure aggravated protein aggregation, shortened lifespan, and worsened age-related decline in motility and pharyngeal pumping. In addition, BaP exposure increased the expression of heat shock proteins and reduced survival under heat stress. However, dietary supplementation with Tangeretin (TAN) alleviated the BaP-induced decline in motility, pumping, and protein accumulation, and restored heat shock proteins' expression levels.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Yen-Chun Koh, Lee-Han Kuo, Yen-Chen Tung, Monthana Weerawatanakorn, Min-Hsiung Pan
Summary: Gut microbial indicators in aged mice have been identified, and these indicators are associated with human diseases and relevant metabolic processes.
Article
Chemistry, Multidisciplinary
Monthana Weerawatanakorn, Sang He, Chun-Han Chang, Yen-Chun Koh, Meei-Ju Yang, Min-Hsiung Pan
Summary: Obesity and overweight pose a growing risk for various health conditions, and diet, especially tea consumption, has been identified as a potential intervention. This study aimed to investigate the effects of GABA oolong tea extracts (OTEs) on high-fat diet (HFD)-induced obesity using male C57BL/6 J mice. The results showed that GABA OTEs reduced leptin expression, protected against fatty liver disease, and promoted thermogenesis and lipid metabolism, therefore preventing obesity development.
Article
Chemistry, Multidisciplinary
Wei-Sheng Lin, Wan-Chen Cheng, Min-Hsiung Pan
Summary: Inflammatory bowel disease (IBD) is a chronic inflammation of the intestines, and Virofree has shown potential in preventing this disease. This study found that Virofree can alleviate intestinal inflammation and modulate the gut microbiota in mice.
Article
Food Science & Technology
Kai-Yu Hsu, Chi-Tang Ho, Min-Hsiung Pan
Summary: Turmeric is a medicinal plant commonly used in Chinese and Indian traditional medicine. It has various health benefits due to the active components called curcuminoids. This article reviews the composition of turmeric and the properties of curcumin, highlighting its antioxidant, anti-inflammatory, anti-diabetic, anti-colorectal cancer, and other physiological activities. It also discusses the challenges of using curcumin and provides three novel application strategies to overcome these limitations.
JOURNAL OF FOOD AND DRUG ANALYSIS
(2023)
Article
Agriculture, Multidisciplinary
Chun Ming How, Ko-Chun Cheng, Yong-Shan Li, Min-Hsiung Pan, Chia-Cheng Wei
Summary: This study finds that chronic exposure to Benzo[a]pyrene (BaP) has negative effects on aging, and Tangeretin (TAN) supplementation can alleviate the aging toxicity induced by BaP.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
