Article
Microbiology
Gongli Zong, Guangxiang Cao, Jiafang Fu, Peipei Zhang, Xi Chen, Wenxiu Yan, Lulu Xin, Wenchi Zhang, Yan Xu, Rongzhen Zhang
Summary: Streptomyces gilvosporeus F607 produces natamycin through a complex regulatory network, including two-component systems. The macR and macS genes play important roles in natamycin biosynthesis and sporulation. Deletion of macRS prevents natamycin production, delays spore formation, and affects septum development. Molecular simulations and experiments suggest that MacR interacts with specific gene regions and promoters to control natamycin biosynthesis and septum formation.
MICROBIOLOGICAL RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Xin Zeng, Chaoping Yue, Zixian Ding, Lulin Wang, Zhiwei Su, Huawei Zeng, Biao Zhang, Feng Li, Mingzhi Zhu
Summary: This study proposes a new disposal approach for waste cooking oil by reusing it as a carbon source for natamycin production. The removal of unsaturated fatty acids through hydrogenation improves natamycin biosynthesis. By using this approach, a significant increase in natamycin production is achieved, along with reduced material costs. It has great significance for industrial natamycin production and environmental protection.
PROCESS BIOCHEMISTRY
(2022)
Article
Biotechnology & Applied Microbiology
Yang Song, Zixuan Zhang, Xiangli Zhang, Jiayi Yao, Xiaoping Yu, Xuping Shentu
Summary: In this study, the production of toyocamycin in Streptomyces diastatochromogenes 1628 was enhanced through three rounds of genome shuffling screening. The optimization of nutrition condition in medium resulted in the highest production of toyocamycin. The mechanism for the improvement of shuffled strains was investigated, revealing the role of transcriptional level, product resistance, and ATP-binding pathway-specific regulator.
FERMENTATION-BASEL
(2022)
Article
Biotechnology & Applied Microbiology
Jayalakshmi Venkatesan, Vasuki Ramu, Thilaga Sethuraman, Chandrasekaran Sivagnanam, Ganesh Doss
Summary: This study successfully developed somatic embryos of finger millet using biotechnological methods and provided methods and hormonal concentrations for its genetic improvement and conservation.
BIOTECHNOLOGY LETTERS
(2022)
Article
Plant Sciences
Zhu Lin, Hamed Khodayari
Summary: Salicornia, a wild halophytic plant, is mainly distributed in coastal areas of Asia, North America, and the Middle East, with cultivation in semi-arid regions of Iran. Research shows that genetic variation in Salicornia persica is relatively low, with some genetic differences among different geographical populations.
Article
Biotechnology & Applied Microbiology
Liting Ye, Ruifang Ye, Fengxian Hu, Guozhu Wang
Summary: FK506, an immunosuppressive polyketide produced by Streptomyces tsukubaensis, showed significantly increased production levels through atmospheric and room temperature plasma mutagenesis and genome shuffling techniques. The addition of exogenous DMSO further enhanced FK506 production. The newly isolated strain R3-C4 exhibited altered morphology and gene expression profiles compared to the wild-type strain, potentially leading to improved FK506 biosynthesis.
BIOTECHNOLOGY LETTERS
(2021)
Article
Biochemistry & Molecular Biology
Ximing Chen, Shuyan Li, Binglin Zhang, Haili Sun, Jinxiu Wang, Wei Zhang, Wenbo Meng, Tuo Chen, Paul Dyson, Guangxiu Liu
Summary: The discovery and functional characterization of a new bacterial tRNA species is reported. This tRNA decodes specific codons and circumvents inefficient base-pairing during translation, leading to increased antibiotic and bioactive metabolite production.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Microbiology
Yongjuan Liu, Kaifang Wang, Long Pan, Xusheng Chen
Summary: In this study, a better ε-PL producing strain was generated through genome shuffling on strains obtained by ribosome engineering. The mutant strain SG-86 produced 144.7% more ε-PL than the parent strain M-Z18. Illumina sequencing was used to map the genomic changes leading to the improved phenotype, revealing a smaller genome size, metabolic pathway changes, and gene deletions in the mutant strain. This study is of great significance for improving fermentation performance and providing theoretical guidance for metabolic engineering of ε-PL producing strains.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Wenliang Hao, Wenjing Cui, Feiya Suo, Laichuang Han, Zhongyi Cheng, Zhemin Zhou
Summary: In this study, a genomic diversification platform was constructed using CRISPR-ABE8e-CDA-nCas9 technology to rapidly evolve bacterial chassis. The platform allows for adjustable conversion efficiency and editable window, enabling editing of any pre-defined genomic loci and generating a diverse repertoire of genomic sequence mutants.
Review
Medicine, General & Internal
Qigang Zhao, Baixue Han, Qian Xu, Tao Wang, Chen Fang, Rui Li, Lei Zhang, Yufang Pei
Summary: The prevalence of obesity has been increasing globally, with genetic factors playing a significant role in obesity predisposition. Integration of genomic and proteomic data can help bridge the gap in understanding genetic variant-obesity associations and identify potential drug targets for obesity treatment.
CHINESE MEDICAL JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Alison H. Nguyen, Weixiang Wang, Emily Chong, Kamalakar Chatla, Doris Bachtrog
Summary: By studying the Y chromosomes in fruit flies, it was found that the differences between Y chromosomes are mainly caused by a few active transposable elements, which have recently expanded on the largest Y chromosome. Different elements are responsible for the expansion of Y on differently sized chromosomes.
Article
Plant Sciences
Yinan Liu, Jiaqing Wang, Hongling Kang
Summary: This study collected and analyzed seven Malva species from seven provinces in Iran, investigating their quantitative morphological characters and genetic diversity. The results showed significant differences in morphological characters and a correlation between genetic and geographical distances in Malva species.
Article
Microbiology
Peng Liu, Yuxin Li, Youyuan Ye, Jiaxin Chen, Rong Li, Qinyi Zhang, Yuan Li, Wen Wang, Qingguo Meng, Jingyu Ou, Zhujun Yang, Wei Sun, Wei Gu
Summary: This study analyzed the whole genome and antigen proteins of Spiroplasma mirum, a small motile wall-less bacteria. The genome analysis revealed various metabolic pathways and ongoing genome reduction. Immunoproteomics was used to identify proteins related to virulence in S. mirum.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Alejandro Ceron-Noriega, Miguel V. Almeida, Michal Levin, Falk Butter
Summary: Nematodes are a diverse group of animals with over 24,000 described species, but most of them lack comprehensive gene annotations. This study used proteotranscriptomics to improve gene annotations for nine genome-sequenced nematode species and provide new gene annotations for three additional species without genomes. The methodology was validated by identifying two previously undescribed genes in the model organism Caenorhabditis elegans. Extensive analysis of the comprehensive proteome annotation provided new insights into the evolutionary processes of this metazoan group.
Article
Endocrinology & Metabolism
Renata Finelli, Sara Darbandi, Peter Natesan Pushparaj, Ralf Henkel, Edmund Ko, Ashok Agarwal
Summary: This study utilized a combination of proteomics analysis and in vitro validation to identify altered expression of proteins involved in DNA repair in varicocele patients, with ACO2 and FASN being highlighted as potential contributors to DNA damage in varicocele pathophysiology.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Materials Science, Biomaterials
Shan Gao, Wanlin Zhang, Xingxing Zhai, Xue Zhao, Jianxin Wang, Jie Weng, Jianshu Li, Xingyu Chen
Summary: Researchers have developed a double-layer drug-loaded microneedle patch with antibacterial and angiogenesis-promoting properties, which can accelerate the healing of diabetic wounds.
BIOMATERIALS SCIENCE
(2023)
Article
Materials Science, Biomaterials
Xinyuan Xu, Peiyu Qiu, Mingyang Sun, Jun Luo, Peng Yu, Libang He, Jianshu Li
Summary: Composites have been widely used in various industries, especially in the dental and orthopedic fields. However, the traditional methods of improving mechanical strength and visibility have drawbacks such as complexity, poor distribution, and long production times. Therefore, developing an integrated material with both reinforced fillers and radiopacity is of great economic and social significance.
JOURNAL OF MATERIALS CHEMISTRY B
(2023)
Article
Materials Science, Biomaterials
Cheng Cheng, Jiayun Ma, Jinling Zhao, Haiying Lu, Yang Liu, Chuanshi He, Man Lu, Xiaohong Yin, Jianshu Li, Mingming Ding
Summary: Based on disulfide-enriched multiblock copolymer vesicles, this study presents a straightforward sequential drug delivery system with dual-redox response that releases hydrophilic doxorubicin hydrochloride (DOX center dot HCl) and hydrophobic paclitaxel (PTX) under oxidative and reductive conditions, respectively. When compared to concurrent therapeutic delivery, the spatiotemporal control of drug release allows for an improved combination antitumor effect. The simple and smart nanocarrier has promising applications in the field of cancer therapy.
JOURNAL OF MATERIALS CHEMISTRY B
(2023)
Article
Nanoscience & Nanotechnology
Yongping Lu, Wenjin Ye, Weiqi Kang, Shuaibing Wang, Zhongqiang Zhu, Xingyu Chen, Jianshu Li
Summary: In this study, a low-cost and highly efficacious wound-healing material, keratin-hyperbranched polymer hydrogel-M (KHBP-M), was developed by mixing reduced keratin, hyperbranched polymer (HBP), and MnO2 nanoparticles. The composite gel showed antibacterial effects against both Gram-positive and Gram-negative bacteria and had excellent ROS-scavenging ability. In an animal model of infected wounds, the KHBP-M hydrogel treated with near-infrared light exhibited the fastest wound-healing rate, reaching 82.98% on day 15.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Review
Biochemistry & Molecular Biology
Kai Xu, Jie Weng, Jianshu Li, Xingyu Chen
Summary: Microneedles (MNs) are a new drug delivery method that has gained attention for their effectiveness, safety, and painlessness. To improve their application, the concept of intelligent stimuli-responsive MNs has been proposed. These MNs can exhibit unique biomedical functions according to changes in the internal and external environment.
MACROMOLECULAR BIOSCIENCE
(2023)
Article
Nanoscience & Nanotechnology
Shuqin Cao, Yuyu Li, Luxuan Shen, Bin Shao, Leixiao Yu, Jianshu Li, Quan Yuan
Summary: This study used plant virus-based nanoparticles to modify a therapeutic peptide RM for the treatment of osteoporosis. The nanoparticles showed excellent biocompatibility and stability, enhancing cellular uptake and inhibiting osteoclastogenesis. The modified nanoparticles also achieved bone enrichment and suppressed bone resorption in mice, providing a promising therapeutic strategy for osteoporosis.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Nanoscience & Nanotechnology
Qiangwei Xin, Zhengxin Ma, Shiran Sun, Hongbo Zhang, Yan Zhang, Liangrui Zuo, Yifei Yang, Jing Xie, Chunmei Ding, Jianshu Li
Summary: Researchers have developed an antifouling self-healing coating for orthodontic appliances. The coating contains hydrophilic and hydrophobic molecules, which give the polymer a structure that repels bacteria while improving adhesion. The coating has the ability to self-heal and is biologically safe, making it a promising solution for improving orthodontic appliance performance and oral health maintenance.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Chemistry, Multidisciplinary
Peng Yu, Yanyan Li, Hui Sun, Hongbo Zhang, Han Kang, Peng Wang, Qiangwei Xin, Chunmei Ding, Jing Xie, Jianshu Li
Summary: A photothermal dual-bionic nanozyme, mimicking antioxidases and hyaluronan synthase, has been developed for effective osteoarthritis (OA) therapy. It can produce heat, scavenge free radicals, and promote chondrogenesis even under inflammatory conditions. This nanozyme regulates anabolic and catabolic processes simultaneously, thereby effectively restoring joint homeostasis and demonstrating an admirable photothermally therapeutic effect on OA.
ADVANCED MATERIALS
(2023)
Review
Chemistry, Multidisciplinary
Yongping Lu, Linming Fan, Jun Wang, Mingxiang Hu, Baogang Wei, Ping Shi, Jianshu Li, Jinyan Feng, Yu Zheng
Summary: The nanodelivery system has limitations in its effectiveness due to short biological half-life and inadequate tumor targeting. The integration of cancer cell membranes (CCM) and nanoparticles has led to the emergence of CCM-based nanoplatforms (CCM-NPs), which have gained significant attention for their unique properties. CCM-NPs not only prolong the blood circulation time of core nanoparticles, but also target tumors effectively. This article discusses the history and development of CCM-NPs, as well as their applications in biomedical research, particularly in cancer therapy.
Review
Chemistry, Multidisciplinary
Xiaoning Sun, Chunmei Ding, Meng Qin, Jianshu Li
Summary: Hydrogel-based biosensors are highly promising for the detection and monitoring of bacterial infections. This review summarizes the recent advancements in this field and discusses the challenges and future development of hydrogel biosensors for bacterial infections.
Article
Nanoscience & Nanotechnology
Qiangwei Xin, Zhengxin Ma, Shiran Sun, Hongbo Zhang, Yan Zhang, Liangrui Zuo, Yifei Yang, Jing Xie, Chunmei Ding, Jianshu Li
Summary: In orthodontic treatment, bacterial infections are a risk to oral health. A self-healing coating based on supramolecular bonding was prepared to improve the antifouling properties of orthodontic appliances. The coating showed excellent antimicrobial activity, surface antifouling properties, and the ability to restore antibiofouling properties at oral temperature and humidity.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Chemistry, Multidisciplinary
Miao Zhang, Xu Peng, Yuan Ding, Xiang Ke, Kai Ren, Qiangwei Xin, Meng Qin, Jing Xie, Jianshu Li
Summary: Enhanced joint synergistic lubrication combined with anti-inflammatory therapy is an effective strategy to delay the progression of early osteoarthritis. A pH-responsive cyclic brush zwitterionic polymer (CB) with high drug-loading efficiency was developed, demonstrating superlubrication, sequence controlled release, and anti-inflammatory effects. The CB shows promise as a long-acting lubricating therapeutic agent for OA treatment or other diseases.
MATERIALS HORIZONS
(2023)
Review
Materials Science, Biomaterials
Yongping Lu, Xinyuan Xu, Jianshu Li
Summary: Adhesive materials, both natural and synthetic, have the ability to stick to mucus or epithelial cells. They are widely used in biomedicine due to their unique properties, such as adhesion, biocompatibility, and surface characteristics. They can target specific areas in the human body, improving therapeutic effects and preventing bacterial infections. This review systematically introduces the properties and mechanisms of different types of adhesive materials and explores their applications in drug delivery, antibacterials, tissue repair, and other biomedical fields. The prospects and challenges of using adhesive materials in biomedicine are also discussed.
JOURNAL OF MATERIALS CHEMISTRY B
(2023)
Article
Engineering, Chemical
Haiqin Tang, Qi Chen, Xiang Ke, Hao Wang, Moyan Li, Jing Xie, Jun Luo, Jianshu Li
Summary: This study proposes a strategy to prepare pH-responsive reversible wet adhesive hydrogels using the structural advantage of urushiol. The resulting hydrogel demonstrates reliable and universally applicable pH-responsive reversible adhesion, as well as remarkable improvements in mechanical properties.
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
(2023)
Article
Materials Science, Biomaterials
Qi Chen, Xiang Ke, Yusong Cai, Hao Wang, Zhiyun Dong, Xinlong Li, Jinlin Li, Xinyuan Xu, Jun Luo, Jianshu Li
Summary: This study introduces a biocompatible small molecule with a triple hydrogen bonding cluster into a polyacrylamide hydrogel, resulting in a highly stretchable hydrogel with superior mechanical properties and adhesion capacity. Furthermore, by incorporating calcium ions, the performance of the hydrogel in skin sensors is significantly improved.
JOURNAL OF MATERIALS CHEMISTRY B
(2023)
