Article
Chemistry, Analytical
Jie Fei, Jiaqun Jiang, Yuchen Bai, Weilin Wu, Xiangjun Zhao, Wenbo Yu, Kai Wen, Xuezhi Yu, Jianzhong Shen, Zhanhui Wang
Summary: This study demonstrates the importance of careful hapten design and extensive antibody screening in the development of sandwich immunoassays for small molecules, using ABM as an example. Through combinatorial associations of antibody pairs, a sandwich immunoassay for ABM was successfully established.
ANALYTICAL CHEMISTRY
(2023)
Article
Materials Science, Multidisciplinary
Bo Kyeong Yoon, Tun Naw Sut, Ki Yeol Yoo, Seung Hwa Lee, Youngkyu Hwang, Joshua A. Jackman, Nam-Joon Cho
Summary: A streamlined ELISA strategy using lipid nanoparticles as a blocking agent was developed, showing fewer processing steps, quicker protocol time, and superior detection performance compared to conventional BSA blocking. This method has been successfully applied to COVID-19 antibody detection and is broadly applicable for rapid ELISA diagnostics development.
APPLIED MATERIALS TODAY
(2021)
Article
Chemistry, Analytical
Hui Li, Menglu Li, Yucai Yang, Fei Wang, Feng Wang, Chao Li
Summary: A direct method has been described to establish the correlation between non-nucleic acid analytes and the CRISPR/Cas12a system using rationally designed DNA strands, enabling ultrasensitive detection of biomarkers from different species. The signal output is highly optional and the sensing principle is akin to ELISA, allowing for direct application on the currently available ELISA platform, further facilitating its use in medical diagnostics.
ANALYTICAL CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Youhee Heo, Kwanwoo Shin, Min Cheol Park, Ji Yoon Kang
Summary: This report introduces a method for enhancing the sensitivity of chemifluorescence-based ELISA, using photooxidation-induced fluorescence amplification (PIFA). PIFA utilizes autocatalytic photooxidation to amplify the fluorescent product, improving detection sensitivity. The method requires minimal modifications to the fluorescence reader and is compatible with conventional ELISA assays.
SCIENTIFIC REPORTS
(2021)
Article
Chemistry, Applied
Long Xu, Fanfan Yang, Alberto C. P. Dias, Xiaoying Zhang
Summary: Sunset yellow is a commonly used food colorant, but excessive and illegal use may pose health risks. Researchers have applied quantum dots in the analysis of sunset yellow and developed two new detection methods. These methods are efficient, highly sensitive, and capable of distinguishing sunset yellow from analogues.
Article
Biochemical Research Methods
S. Delshadi, M. Fratzl, O. Ramel, P. Bigotte, P. Kauffmann, D. Kirk, V. Masse, M. P. Brenier-Pinchart, H. Fricker-Hidalgo, H. Pelloux, F. Bruckert, C. Charrat, O. Cugat, N. M. Dempsey, T. Devillers, P. Halfon, A. Leroy, M. Weidenhaupt, P. N. Marche
Summary: The magnetically localized and wash-free fluorescence immunoassay (MLFIA) is a novel detection method that directly measures the concentration of biomolecules without the need for mixing or washing steps. It uses magnetic nanoparticles to capture specific biomolecules and quantitatively measure their numbers on magnet lines, without any washing steps. The MLFIA platform has been evaluated in various biological models and clinical blood samples, with benchmarking to standard analyzers of healthcare laboratories, showing promising performances in the quantitative detection of various targets in different biological samples for different clinical tests.
Article
Chemistry, Applied
Xinzhu Chen, Huaying Du, Yanping Hong, Yubo Wang, Mei Shu, Dan Wang, Peng Dai, Weijie Deng, Jianhua Xiong, Wuying Yang
Summary: A dcELISA method based on scFv-AP fusion protein was developed for detecting AMOZ residues in ctenopharyngodon idellus. The method showed high sensitivity and accuracy, enabling rapid detection of AMOZ residues in fish samples.
FOOD AND AGRICULTURAL IMMUNOLOGY
(2021)
Article
Multidisciplinary Sciences
Amani A. Hariri, Sharon S. Newman, Steven Tan, Dan Mamerow, Alexandra M. Adams, Nicolo Maganzini, Brian L. Zhong, Michael Eisenstein, Alexander R. Dunn, H. Tom Soh
Summary: One major challenge in enzyme-linked immunosorbent assays (ELISAs) is distinguishing true signal from non-specific binding. The authors developed a Single-Molecule Colocalization Assay (SiMCA) that eliminates this issue and allows for reproducible detection of picomolar protein concentrations.
NATURE COMMUNICATIONS
(2022)
Article
Chemistry, Multidisciplinary
Jianlei Shen, Bo Situ, Xianchao Du, Zhiming Wang, Rong Hu, Baixue Li, Anjun Qin, Ben Zhong Tang
Summary: This study presents a strategy to improve the detection sensitivity and broaden the dynamic range of enzyme-linked immunosorbent assay (ELISA) by replacing traditional chromogenic reagents with an aggregation-induced emission luminogen (AIEgen). The developed AIE-ELISA showed a lower limit of detection and a broader dynamic range compared to single-mode ELISA in the detection of prostate-specific antigen (PSA), allowing for differentiation of minor PSA differences in patient's serum. The AIE-ELISA demonstrated simpler experimental operation, faster enzyme response speed, and better photostability than traditional chromogenic reagents, making it highly promising in the fields of immunoassay, immunohistochemistry, and immunocytochemistry.
Article
Biotechnology & Applied Microbiology
Xinyang Li, Miyang Li, Shengbao Duan, Hongmei Wang, Yong Li, Tiemei Liu
Summary: The objective of this study is to develop a simple, rapid, reliable, sensitive, and cost-effective method for prenatal detection of fetomaternal haemorrhage by combining a multi-aperture silk membrane with enzyme-linked immunosorbent assay (ELISA). This method does not require any complicated instruments and can be visually colored, providing a new method for clinical detection of fetomaternal haemorrhage.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Chemistry, Analytical
Eunice Y. Kwon, Haneen A. Abusharkh, Xiaofan Ruan, Dan Du, Ellis Hammond-Pereira, Bernard J. Van Wie
Summary: A new quantifying nanoparticle sandwich immunoassay technique for collagen type II was developed using mesoporous palladium@platinum (Pd@Pt) nanoparticles as enzyme substitutes. This method showed a low limit of detection, a broad linear range, and a high relative standard deviation, and can be used at different pH values.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Applied
Fan He, Daoping Zeng, Jinyi Yang, Yuanxin Tian, Yudong Shen
Summary: Veterinary drug residues in food-producing animals pose a threat to human health and public safety. Current antibodies produced to detect these residues have limited accuracy and sensitivity. In this study, a new antigen strategy was adopted to produce a monoclonal antibody that could directly recognize the metabolite without derivatization, resulting in excellent analytical performance.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2024)
Article
Chemistry, Analytical
Zhiyuan Jin, Lei Wang, Dongni Cao, Siyi Zou, Chaoqun Chen, Huimei Kang, Qifang Song, Hong Wang, Yong Tang
Summary: A new method based on sandwich cell surface fluorescence immunosorbent assay (SCSFIA) was developed for rapid screening of paired antibodies. The method was successfully used to screen antibodies against Avian influenza A (H7N9) virus, demonstrating its applicability for various virus antibody pair screening.
ANALYTICA CHIMICA ACTA
(2021)
Article
Biochemistry & Molecular Biology
Elisabeth Anne Adanma Obara, Birgitte Georg, Jens Hannibal
Summary: Pituitary adenylate cyclase-activating polypeptide (PACAP) plays a crucial role in migraine pathophysiology. We have developed a sandwich ELISA assay capable of detecting and accurately quantifying PACAP.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Lucia Vieira Hoffmann, Amanda Alves Branquinho, Paulo Augusto Vianna Barroso, Maite F. S. Vaslin
Summary: By developing monoclonal and polyclonal antibodies based on coat protein peptides, an efficient ELISA detection method was established to detect the cotton blue disease virus in cotton and weeds. The study has the potential to guide the development of other diagnostic procedures.
FRONTIERS IN PLANT SCIENCE
(2022)
