期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 59, 期 9, 页码 5044-5051出版社
AMER CHEMICAL SOC
DOI: 10.1021/jf2002238
关键词
Cycloamylose; amylosucrase; 4-alpha-glucanotransferase; one-pot synthesis
资金
- Rural Development Administration (RDA), Republic of Korea [20070301034008]
- Sejong University
- Rural Development Administration (RDA), Republic of Korea [20070301034008] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Amylose-like alpha-(1,4)-glucan known as the most favorable substrate for the enzymatic production of cycloamyloses (CAs) using 4-alpha-glucanotransferase has a solubility issue, which requires an additional solubilization process. In our study, two glucosyltransferases, Synechocystis 4-alpha-glucanotransferase and Neisseria amylosucrase, were adopted to develop an efficient biocatalytic production process of CAs directly from sucrose. From one-pot synthesis, the maximum CA yield (9.6%, w/w) with 0.3 M sucrose was achieved with 10 units/mL of amylosucrase and 0.1 unit/mL of 4-alpha-glucanotransferase at 40 degrees C for a 3 h reaction in a simultaneous dual enzyme reaction mode. The size of linear alpha-(1,4)-glucan was positively related to the CA productivity by 4-alpha-glucanotransferase in a hyperbolic manner. Using our innovative bioprocess, there was no practical limitation on the initial sucrose concentration and no use of insoluble linear alpha-(1,4)-glucan substrate. Consequently, the concomitant dual enzyme reaction converted sucrose directly to CAs via in situ transient linear alpha-(1,4)-glucan as an soluble intermediate.
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