Article
Food Science & Technology
Xiya Zhang, Mingyue Ding, Chensi Zhang, Yexuan Mao, Youyi Wang, Peipei Li, Haiyang Jiang, Zhanhui Wang, Xuezhi Yu
Summary: The researchers developed a rapid icELISA method for monitoring brevetoxin levels in oysters, providing a useful tool for the detection of these toxins in shellfish samples.
Article
Chemistry, Applied
Shunsuke Fujii, Tomoe Ohta, Riho Ehama, Mizuki Irikida, Shuichi Nomura, Yukihiro Shoyama, Takuhiro Uto
Summary: This study developed a simple and reliable method for the detection and quantification of FMN in food samples, and successfully applied it in cell-based assays.
Article
Chemistry, Analytical
Haijiao Gao, Guyue Cheng, Hui Wang, Ting Chen, Chao Xu, Houquan Lv, Heying Zhang, Ren Hou, Yulian Wang, Dapeng Peng, Zhonghui Yuan
Summary: A broad-spectrum monoclonal antibody (mAb)-based indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) was developed for rapidly screening androgens in various animal tissues. The method showed good correlation with HPLC-MS/MS results, demonstrating its effectiveness and accuracy for detecting androgens in animal tissues.
MICROCHEMICAL JOURNAL
(2021)
Article
Chemistry, Applied
Lulu Zhou, Guifen Chen, Min Chen, Xinying Lu, Yijie Xi, Yun Zhi
Summary: An indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) using a highly sensitive and specific monoclonal antibody against avilamycin was developed. The optimized ic-ELISA showed low detection limits and good correlation and reliability compared to HPLC.
FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT
(2022)
Article
Chemistry, Applied
Zile Wang, Pimiao Zheng, Jianyi Wang, Shuang He, Zhenhui Ren, Yanfang Zhang, Jincheng Xiong, Haiyang Jiang
Summary: An ic-ELISA method based on a broad-spectrum monoclonal antibody was developed for rapid screening of tropane alkaloids in pig urine, pork, and cereal flour samples. The method showed good sensitivity and reliability, with results correlating well with high performance liquid chromatography-tandem mass spectrometry.
Article
Food Science & Technology
Yanan Wang, Xiaofei Wang, Shuyun Wang, Hanna Fotina, Ziliang Wang
Summary: This study established a highly sensitive and specific ZEN monoclonal antibody (mAb) and an indirect competitive enzyme-linked immunosorbent assay (icELISA) for the detection of ZEN residues in food and feed. The icELISA showed high sensitivity, specificity, rapidity, and reliability in detecting ZEN, making it a valuable tool for ZEN detection.
Article
Food Science & Technology
Jiao Li, Yuan Ding, He Chen, Wanlin Sun, Yue Huang, Fengquan Liu, Minghua Wang, Xiude Hua
Summary: Propiconazole, a commonly used fungicide in agricultural production, presents health risks. An ic-ELISA method using monoclonal antibodies was developed for accurate detection of propiconazole residues, showing high sensitivity and reliability. The results were consistent with gas chromatography-mass spectrometry analysis, providing an alternative tool for propiconazole determination.
Article
Chemistry, Analytical
Shuangmin Wu, Wei Shen, Yushuang Chen, Yongli Zhu, Ren Hou, Menghong Dai, Dapeng Peng
Summary: In this study, a specific anti-cephalexin monoclonal antibody was used to develop an ic-ELISA method for the determination of cephalexin in animal-derived foods. The established method showed high sensitivity and accuracy, and correlated well with HPLC-MS/MS analysis.
ANALYTICAL LETTERS
(2022)
Article
Food Science & Technology
Yu Wang, Jin-Yi Yang, Ying He, Lu Li, Jian-Xin Huang, Yuan-Xin Tian, Hong Wang, Zhen-Lin Xu, Yu-Dong Shen
Summary: A quantitative and visual TRFICA method was developed for detecting T&T residues in milk, with low detection limits, short detection time, and good stability.
Article
Chemistry, Medicinal
Akihiro Ochi, Shunsuke Fujii, Tomoe Ohta, Takuhiro Uto, Poomraphie Nuntawong, Hiroyuki Tanaka, Satoshi Morimoto, Seiichi Sakamoto
Summary: In this study, a new icELISA method based on a highly specific anti-SSb2 monoclonal antibody was developed for the detection of SSb2 in Kampo medicines. The assay showed high specificity, accuracy, and precision, and the results were strongly correlated with those obtained using liquid chromatograph-mass spectrometer. This suggests that the developed method can be used for the quality control and standardization of Kampo medicines containing Bupleuri radix.
JOURNAL OF NATURAL MEDICINES
(2023)
Article
Food Science & Technology
Xianshu Fu, Erjing Chen, Biao Ma, Ying Xu, Peiying Hao, Mingzhou Zhang, Zihong Ye, Xiaoping Yu, Chaofeng Li, Qingge Ji
Summary: Heavy metals in food packaging materials, especially cadmium, are a global environmental threat. This study proposed an ic-ELISA method based on a specific monoclonal antibody for rapid detection of Cd. The ic-ELISA showed high specificity and accuracy in diagnosing Cd in food packaging materials.
Article
Biochemistry & Molecular Biology
Yuan-yuan Yang, Yu Wang, Yi-feng Zhang, Feng Wang, Yi-fan Liang, Jin-yi Yang, Zhen-lin Xu, Yu-dong Shen, Hong Wang
Summary: This study successfully selected three specific nanobodies against 19-NT and developed an ic-ELISA method using Nb2F7 with low detection limit and excellent recovery rates. The technology showed high correlation with the UPLC-MS/MS method.
Article
Biochemical Research Methods
Poomraphie Nuntawong, Taiki Horikawa, Akihiro Ochi, Shinji Wada, Yumi Tsuneura, Hiroyuki Tanaka, Seiichi Sakamoto, Satoshi Morimoto
Summary: The study aimed to produce an anti-SM monoclonal antibody (MAb) and develop an indirect competitive enzyme-linked immunosorbent assay (icELISA) for standardisation and quality control of Swertia japonica. The developed icELISA was sensitive, with a quantitative range of 0.78 to 12.5 μg/mL, and showed good repeatability with coefficients of variation below 10%. The method was accurate, as confirmed by recoveries in a spike and recovery assay ranging from 91.84% to 115.50%, and the SM content measured using the icELISA was consistent with the results of a high-performance liquid chromatography-ultraviolet (HPLC-UV) assay.
PHYTOCHEMICAL ANALYSIS
(2021)
Article
Chemistry, Applied
Yifang Cui, Huiwen Wang, Fangfang Guo, Xiaoya Cao, Xue Wang, Ximin Zeng, Guolin Cui, Jun Lin, Fuzhou Xu
Summary: This study developed a monoclonal antibody-based ELISA method for quantifying Enterobacteriaceae levels in the intestine, which can be used to assess gut inflammation. The ELISA showed high accuracy, repeatability, and reproducibility, and was able to detect Enterobacteriaceae levels produced in different bacterial cultures.
Article
Chemistry, Analytical
Lu Lin, Shanshan Song, Xiaoling Wu, Liqiang Liu, Hua Kuang, Jing Xiao, Chuanlai Xu
Summary: This study developed an ic-ELISA and immunochromatographic strip assay for rapid screening of robenidine hydrochloride (ROBH), showing high specificity and good recovery rates. Both methods yielded results consistent with LC-MS/MS analysis, making them effective for detecting ROBH in shrimp, chicken breast, and chicken liver samples.