Article
Microbiology
Takeshi Kawahara, Issei Shimizu, Yuuki Tanaka, Keisuke Tobita, Mikado Tomokiyo, Itsuki Watanabe
Summary: This study found that SLPs from Lactobacillus crispatus KT-11 can inhibit human rotavirus infection of intestinal epithelial cells. KT-11 LE showed a dose-dependent suppressive effect on DS-1 infection with pre-infection treatment, while KT-11 SLP concentrate significantly inhibited the proliferative process of DS-1 strain. These results provide crucial evidence for the antiviral effect of SLPs.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Keisuke Tobita, Fumika Hoshi, Taeko Ohki, Itsuki Watanabe
Summary: This study investigates the mechanism of IL-12p40 production induced by Lactobacillus crispatus KT-11 strain and found that protein denature extracts promote IL-12p40 production via Toll-like receptor 2.
JOURNAL OF FOOD BIOCHEMISTRY
(2021)
Article
Food Science & Technology
Saber Amiri, Reza Rezaei Mokarram, Mahmoud Sowti Khiabani, Mahmoud Rezazadeh Bari, Mohammad Alizadeh
Summary: This study aimed to optimize the co-production of conjugated linoleic acid (CLA), exopolysaccharides (EPSs) and bacteriocins (BACs) by Lactobacillus acidophilus LA-5, achieving high viable cell number, CLA, EPSs, and inhibition activity at optimal conditions. The analysis revealed the functional groups of pharmabiotics and showed the presence of CLA isomers and the composition of EPSs. SDS page analysis reported BACs molecular weight fractions, demonstrating the potential for simultaneous production of bioactive metabolites by probiotics in supplemented cheese whey.
JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE
(2021)
Article
Biochemistry & Molecular Biology
Syeda Tayyiba Rahat, Mira Makela, Maryam Nasserinejad, Tiina M. M. Ikaheimo, Henna Hyrkas-Palmu, Rasmus I. P. Valtonen, Juha Roning, Sylvain Sebert, Anni I. Nieminen, Nsrein Ali, Seppo Vainio
Summary: Cell-secreted extracellular vesicles (EVs) have the potential to serve as non-invasive solutions for monitoring health and disease. We developed a protocol to purify and characterize sweat EVs from healthy participants and identified metabolites associated with metabolic changes that may reflect correlations with blood glucose and BMI. This study provides a proof-of-concept for using sweat EVs and their metabolites as a non-invasive approach to monitor wellbeing and changes in diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Food Science & Technology
Ting Wang, Zhipeng Xue, Haoran Liu, Ruiting Zhao, Jiahui Peng, Siling Huang, Nan Li, Xueping Guo, Zhenshang Xu
Summary: In this study, a food-grade expression system of L. plantarum was constructed using the lacLM gene and B-galactosidase as a screening marker. The knockout strain AlacAlacM was selected as the host and the lacM gene was successfully expressed using the plasmid pMG36e. A novel food-grade vector pMG36m with lacM gene as a selection marker and lactose as a screening condition was obtained, and an expression vector pMG36m-gfp with gfp gene as the reporter gene was also constructed.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Xi He, MingJian Luan, Ning Han, Ting Wang, Xiangzhong Zhao, Yanyan Yao
Summary: The study investigated the differences in functionality and activity of two beta-galactosidases in Lactobacillus kefiranofaciens, showing distinct roles in lactose hydrolysis and galacto-oligosaccharides production. The findings suggest that the food-grade expression system of recombinant beta-galactosidase could be feasible for applications in the food and dairy industry.
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Food Science & Technology
Fereshteh Falah, Alireza Vasiee, Behrooz Alizadeh Behbahani, Farideh Tabatabaee Yazdi, Seyed Ali Mortazavi
Summary: The study evaluated the production of GABA by Lactobacillus brevis PML1 in a medium containing dairy sludge and soybean meal. Results showed that under optimal conditions, the strain was able to produce 300 ppm GABA, with fermented extract exhibiting antimicrobial, antioxidant, and anti-cancer cell properties. The strain also showed no hemolytic or DNase activity, making it a potential candidate for biological production of GABA in various food and pharmaceutical formulations.
FOOD SCIENCE & NUTRITION
(2021)
Article
Food Science & Technology
Daniela Marianne Hartl, Oliver Frank, Corinna Dawid, Thomas Frank Hofmann
Summary: The focus of green chemistry on natural deep eutectic solvents (NADES) is due to their sustainability, low toxicity, and high solute potential. NADES are applied in various fields, including organic chemistry, agriculture, and food industry. In the food industry, NADES can be used for extraction of small molecules, macromolecules, and heavy metals to achieve desired food quality. Additionally, NADES can increase compound yield in Maillard-type model reactions. A food-grade NADES system based on sucrose/D-sorbitol was developed and evaluated for its stability as a reaction medium in terms of temperature and pH. Two Maillard-type model reactions were successfully implemented, producing taste-modulating compounds that exceed their respective thresholds. This newly developed NADES shows great potential as a solvent candidate for a wide range of applications in the food industry.
Article
Microbiology
K. Tobita, I Watanabe, M. Tomokiyo, M. Saito
BENEFICIAL MICROBES
(2018)
Article
Agriculture, Dairy & Animal Science
Keisuke Tobita, Hiroyuki Yanaka, Hajime Otani
ANIMAL SCIENCE JOURNAL
(2010)
Article
Agriculture, Multidisciplinary
Keisuke Tobita, Hiroyuki Yanaka, Hajime Otani
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2009)
Article
Nutrition & Dietetics
Keisuke Tobita, Hiroyuki Yanaka, Hajime Otani
JOURNAL OF NUTRITIONAL SCIENCE AND VITAMINOLOGY
(2010)
Article
Biochemistry & Molecular Biology
Keisuke Tobita, Fumika Hoshi, Taeko Ohki, Itsuki Watanabe
Summary: This study investigates the mechanism of IL-12p40 production induced by Lactobacillus crispatus KT-11 strain and found that protein denature extracts promote IL-12p40 production via Toll-like receptor 2.
JOURNAL OF FOOD BIOCHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Keisuke Tobita, Ryusuke Meguro
Summary: The Bacillus subtilis BN strain promotes Th1 response in mouse macrophages by inducing M1 polarization via Toll-like receptor 2 signaling pathway. This study serves as a significant reference for the development of functional food and feed with immunostimulatory effects. Potential new food and feed products containing the BN strain, such as juice, powder, and tablets, may emerge in the future.
JOURNAL OF FOOD BIOCHEMISTRY
(2022)
Article
Microbiology
Takeshi Kawahara, Issei Shimizu, Yuuki Tanaka, Keisuke Tobita, Mikado Tomokiyo, Itsuki Watanabe
Summary: This study found that SLPs from Lactobacillus crispatus KT-11 can inhibit human rotavirus infection of intestinal epithelial cells. KT-11 LE showed a dose-dependent suppressive effect on DS-1 infection with pre-infection treatment, while KT-11 SLP concentrate significantly inhibited the proliferative process of DS-1 strain. These results provide crucial evidence for the antiviral effect of SLPs.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Food Science & Technology
Keisuke Fujii, Yuji Kubo, Tomotsugu Noguchi, Keisuke Tobita
Summary: This study investigated the antiviral effects of Bacillus subtilis natto in a human cell culture model mimicking ssRNA viral infection. The XF36 strain of B. subtilis natto induced the expression of genes associated with antiviral and anti-inflammatory activities, suggesting its potential in suppressing viral infections and excessive inflammation.
Article
Microbiology
Keisuke Tobita, Itsuki Watanabe, Masanori Saito
BIOSCIENCE OF MICROBIOTA FOOD AND HEALTH
(2017)
Article
Agriculture, Multidisciplinary
Keisuke Tobita, Takeshi Kawahara, Hajime Otani
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2006)