Article
Biochemistry & Molecular Biology
Lei Song, Xiao-Chen Wang, Zhen-Quan Feng, Yan-Feng Guo, Guo-Qing Meng, Hai-Ying Wang
Summary: In this study, the tannin-degrading strain Debaryomyces hansenii was screened and the tannase coding gene TANF was successfully cloned and expressed in Yarrowia lipolytica. The purified TanF tannase showed optimal activity at 40 degrees C and retained over 80% of its activity in the range of 35 degrees C-60 degrees C. It exhibited remarkable enzyme activity at pH 5.0 and retained more than 70% of its relative activity across a wide pH range of 3.0-8.0. TanF also demonstrated broad substrate specificity for gallate esters and achieved high gallic acid production from tannic acid.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2023)
Article
Plant Sciences
Ko Tahara, Mitsuru Nishiguchi, Evelyn Funke, Shin-Ichi Miyazawa, Takafumi Miyama, Carsten Milkowski
Summary: The enzymes EcDQD/SDH2 and 3 from Eucalyptus camaldulensis have been identified to catalyze gallate formation, which is essential for the biosynthesis of the aluminum-detoxifying metabolite oenothein B.
Article
Plant Sciences
Martina Jakovljevic Kovac, Stela Jokic, Igor Jerkovic, Maja Molnar
Summary: This study extracted gallic acid, ellagic acid, and hydrolyzable tannins (HT) from Alchemilla vulgaris L. plant using different deep eutectic solvents (DESs). Choline chloride:urea (1:2) was found to be the most suitable solvent. Extraction by stirring and heating was the most effective method for obtaining the highest amounts of gallic acid, ellagic acid, and HT.
Article
Biochemistry & Molecular Biology
Yuji Tanaka, Makoto Tsuneoka
Summary: This study found that propyl gallate (PG) and epigallocatechin gallate (EGCG) reduce rRNA transcription and cell proliferation in MCF-7 cells by activating KDM2A. Activation of AMPK and ROS production by PG or EGCG is necessary for KDM2A activation. The inhibition of rRNA transcription and cell proliferation by these compounds is specifically observed in MCF-7 cells.
Article
Biotechnology & Applied Microbiology
Moataza Mahmoud Saad, Abdelnaby Mahmoud Saad, Helmy Mohamed Hassan, Eman I. Ibrahim, Mohamed Abdelraof, Basant A. Ali
Summary: Tannases are valuable enzymes used in various industries. This study isolated 15 fungal strains from Egyptian soil and marine samples and screened them for tannase production. The highest tannase activity was found in Aspergillus glaucus. Black tea waste was identified as the best substrate for tannase production by Aspergillus glaucus. Optimal conditions for enzyme production were identified and a quadratic model was designed for further optimization studies. The efficiency of tannic acid conversion to gallic acid was evaluated, and a bioconversion efficiency of 38.27 mg/mL was achieved. The importance of A. glaucus tannase in industrial applications was demonstrated.
BIORESOURCES AND BIOPROCESSING
(2023)
Article
Biochemistry & Molecular Biology
Rippin, Anil Sharma, Vikas Beniwal
Summary: Condensed and hydrolyzable tannins in various fruits can be hydrolyzed by tannase enzyme, resulting in the production of gallic acid and tannase-mediated products with potential therapeutic benefits. Prunus domestica and Plum had the highest phenol and hydrolyzable tannin contents, respectively. The hydrolysis of fruit extracts led to increased antioxidant and antibacterial activities.
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
(2023)
Review
Biotechnology & Applied Microbiology
Martha Gabriela Garcia Mendez, Thelma Karina Morales Martinez, Juan Alberto Ascacio Valdes, Monica Lizeth Chavez Gonzalez, Adriana Carolina Flores Gallegos, Leonardo Sepulveda
Summary: This review examines the release of tannase and other metabolites through bacterial fermentation processes, highlighting the importance of bacterial fermentation in the food industry and its potential health benefits.
FERMENTATION-BASEL
(2021)
Article
Pharmacology & Pharmacy
Adriana Ribeiro, Rita Serrano, Isabel B. Moreira da Silva, Joao F. Pinto, Olga Silva
Summary: The study developed adhesive tablets containing DVR for the treatment of oral cavity diseases. Chemical analysis and performance evaluation demonstrated the preparation method and performance of the tablets.
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2023)
Article
Plant Sciences
Jianhua Wang, Ketao Wang, Shiheng Lyu, Jianqin Huang, Chunying Huang, Yulin Xing, Yige Wang, Yifan Xu, Peipei Li, Junyan Hong, Jianwei Xi, Xiaolin Si, Hongyu Ye, Yan Li
Summary: The study identified seven tannase genes responsible for tannin metabolism from walnut, pecan, and Chinese hickory in the Juglandaceae family, showing higher expression in leaves and quick up-regulation after leaf injury. The expression of synthesis-related genes decreased during seed coat development, leading to reduced tannin content in Chinese hickory, while fresh samples showed increased tannin content due to water content reduction during ripening. Differences in tannin content and astringent taste between Chinese hickory and pecan were attributed to gene expression variations.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Plant Sciences
Yifan Chen, Changjuan Jiang, Shixin Yin, Juhua Zhuang, Yue Zhao, Lingjie Zhang, Xiaolan Jiang, Yajun Liu, Liping Gao, Tao Xia
Summary: In this study, it was discovered that tannase in tea plants is not only a hydrolase, but also an acyltransferase. Through enzyme and metabolic analysis, it was found that overexpression of tannase increased the total contents of simple galloyl glucoses and flavan-3-ol gallates in strawberry, tea, and Chinese bayberry.
Review
Biochemistry & Molecular Biology
Marco Govoni, Francesca Danesi
Summary: This scoping review provides an overview of the therapeutic potential of hydrolyzable tannins (HTs) and their metabolites in preventing or alleviating the course of osteoarthritis (OA). Although further clinical trials are needed to unravel the potential benefits of these compounds, these studies offer a new dietary strategy for improving OA.
Article
Engineering, Chemical
Pattarabhorn Pakaweerachat, Teerin Chysirichote
Summary: This research aimed to study the production of gallic acid by tannase from Aspergillus niger ATCC 16888 in solid-state fermentation using Triphala waste. Results showed that Triphala waste was a good substrate for tannase and gallic acid production. Nitrogen supplementation and aeration rate were important parameters for tannase production, and the carbon/nitrogen ratio of the substrate also influenced tannase production.
CHEMICAL ENGINEERING COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Hyeyeong Seo, Seok-Hee Lee, Yooheon Park, Hee-Seok Lee, Jeong Sup Hong, Cho Young Lim, Dong Hyeon Kim, Sung-Soo Park, Hyung Joo Suh, Ki-Bae Hong
Summary: The study found that tannase-treated green tea extract can significantly increase muscle mass and strength without concurrent exercise, providing therapeutic benefits for age-related muscle function decline.
Article
Biotechnology & Applied Microbiology
Xiang Tang, Chun -Li Liu, Yi-Ying Chen, Yi Wei, Xiao-Yan Zhuang, Qiong Xiao, Jun Chen, Fu-Quan Chen, Qiu-Ming Yang, Hui-Fen Weng, Bai-Shan Fang, Yong-Hui Zhang, An-Feng Xiao
Summary: This study proposed a novel strategy for the simultaneous extraction and enzymatic hydrolysis of tara gallotannins. By constructing a thermostable tannase, the production efficiency of gallic acid (GA) was significantly improved, while reducing pollution to the environment.
BIOCHEMICAL ENGINEERING JOURNAL
(2022)
Article
Energy & Fuels
Shagufta Saeed, Iram Bibi, Tahir Mehmood, Rahat Naseer, Muhammad Bilal
Summary: In solid-state fermentation, black plum leaves showed high yields of tannase and gallic acid, with the best production achieved at a substrate water ratio of 1:2, pH 5.5, and temperature 30℃. The addition of glucose and nitrogen source increased the yields. The commercial production of tannase and gallic acid from plant leaves through solid-state fermentation in an optimized environment has significant economic importance.
BIOMASS CONVERSION AND BIOREFINERY
(2022)
Article
Biotechnology & Applied Microbiology
Jose Miguel Mancheno, Estibaliz Atondo, Julen Tomas-Cortazar, Jose Luis Lavin, Laura Plaza-Vinuesa, Itziar Martin-Ruiz, Diego Barriales, Ainhoa Palacios, Claudio Daniel Navo, Leticia Sampedro, Ainize Pena-Cearra, Miguel Angel Pascual-Itoiz, Janire Castelo, Ana Carreras-Gonzalez, Donatello Castellana, Aize Pellon, Susana Delgado, Patricia Ruas-Madiedo, Blanca delas Rivas, Leticia Abecia, Rosario Munoz, Gonzalo Jimenez-Oses, Juan Anguita, Hector Rodriguez
Summary: The occurrence and development of colorectal cancer is associated with the presence of Fusobacterium nucleatum, dietary components, and reduction of acetylated derivatives of spermidine. The study found that a new tannase orthologue of F. nucleatum (TanBF(nn)) has structural differences affecting the active site and substrate accessibility. Spermidine and its derivatives inhibit the hydrolytic activity of TanBF(nn) and increase the toxicity of gallotannins to F. nucleatum.
MICROBIAL BIOTECHNOLOGY
(2022)
Article
Food Science & Technology
Ana Ruiz de la Bastida, Angela Peiroten, Susana Langa, Jose Antonio Curiel, Juan Luis Arques, Jose Maria Landete
Summary: Fermenting soy beverages with bacterial strains Limosilactobacillus mucosae INIA P508 and Bifidobacterium pseudocatenulatum INIA P815 can increase the concentration of isoflavone aglycones and other bioactive flavonoids. Cold storage and thermal treatment do not impact the levels of isoflavones, allowing these functional beverages to maintain their nutrients under different storage conditions.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Eduardo Rosado, Paloma Delgado-Fernandez, Blanca de las Rivas, Rosario Munoz, Francisco Javier Moreno, Nieves Corzo, Cesar Mateo
Summary: This study presents the synthesis of beta-galactosyl xylitol derivatives using immobilized LacA beta-galactosidase from Lactobacillus plantarum WCFS1. The immobilized enzyme exhibited high activity and stability, allowing for the synthesis of a large amount of beta-galactosyl xylitol derivatives with improved efficiency. It also showed lower in vitro intestinal digestibility compared to lactose.
Review
Biochemistry & Molecular Biology
Felix Lopez de Felipe, Blanca de las Rivas, Rosario Munoz
Summary: This review summarizes the molecular mechanisms involved in the responses of Lactobacillus spp. to plant phenolic compounds (PPCs). Lactobacilli are more tolerant to PPCs compared to other bacteria and induce a wide variety of mechanisms to increase oxidative stress response. Additionally, PPCs can modulate molecular functions used by lactobacilli to adapt to host environments.
Article
Food Science & Technology
Jose Antonio Curiel, Jose M. Landete
Summary: It was found that only some lactic acid bacteria strains were able to transform biochanin A into genistein, and no strains could demethylate formononetin. By cloning the dmt734 gene from Bifidobacterium breve INIA P734 into LAB and bifidobacteria, biochanin A was successfully converted into genistein.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Gastroenterology & Hepatology
Maria Juarez-Fernandez, Naroa Goikoetxea-Usandizaga, David Porras, Maria Victoria Garcia-Mediavilla, Miren Bravo, Marina Serrano-Macia, Jorge Simon, Teresa C. Delgado, Sofia Lachiondo-Ortega, Susana Martinez-Florez, Oscar Lorenzo, Mercedes Rincon, Marta Varela-Rey, Leticia Abecia, Hector Rodriguez, Juan Anguita, Esther Nistal, Maria Luz Martinez-Chantar, Sonia Sanchez-Campos
Summary: This study provides evidence for the relevance of the interaction between mitochondria and microbiota in NASH and identifies targeting this interaction as a valuable therapeutic approach.
Article
Biochemistry & Molecular Biology
Guillermo Cruz, Javier Acosta, Jose Miguel Mancheno, Jon Del Arco, Jesus Fernandez-Lucas
Summary: This study presents a structure-guided approach to develop novel and thermostable 2'-deoxyribosyltransferases (NDTs) using computational design of disulfide bonds. The most thermostable variant, LdNDT(S104C), showed enhanced stability at high temperatures and broad activity across different temperature and pH ranges. Furthermore, it demonstrated improved catalytic performance in the enzymatic synthesis of nelarabine.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Agriculture, Multidisciplinary
Laura Plaza-Vinuesa, Oswaldo Hernandez-Hernandez, Ana Sanchez-Arroyo, Jose M. Cumella, Nieves Corzo, Ana M. Munnoz-Labrador, F. Javier Moreno, Blanca de las Rivas, Rosario Munoz
Summary: This study confirmed the myrosinase-like activity of Lactiplantibacillus plantarum WCFS1, indicating that glucosinolates in this strain may undergo phosphorylation and the glycosidases belonging to the GH1 family are involved in the hydrolysis of phosphorylated glucosinolates.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Biochemical Research Methods
Ruben Prieto-Paredes, Jose Maria Landete, Angela Peiroten, Jose Antonio Curiel, Susana Langa
Summary: Through multiple sequence alignments, we found that the ability of some lactic acid bacteria (LAB) strains to synthesize riboflavin is related to the presence of ribG, ribB, ribA, and ribH genes in the rib operon. However, these gene sequences are not identical among different LAB species, making primers designed for one species ineffective for others. Therefore, we developed degenerate primers based on sequences from specific LAB species and established optimal PCR conditions for detecting rib genes in different LAB species. By using these primers, we successfully detected ribG, ribB, ribA, and ribH genes in riboflavin-producing LAB strains of different species, suggesting their potential as candidates for producing riboflavin-enriched functional foods.
JOURNAL OF MICROBIOLOGICAL METHODS
(2023)
Article
Immunology
Michelle J. Klouwens, Jos J. A. Trentelman, Diego Barriales, Jasmin I. Ersoz, Mikel Azkargorta, Felix Elortza, Radek Sima, Ondrej Hajdusek, Julen Tomas Cortazar, Iraide Escobes Corcuera, Emil Colstrup, Abhijeet Nayak, Itziar Martin Ruiz, Hector Rodriguez, Ard M. Nijhof, Juan Anguita, Joppe W. R. Hovius
Summary: In this study, differential protein production in the salivary glands of Ixodes ricinus ticks in response to Borrelia afzelii infection and different feeding conditions was identified using quantitative proteomics. Recombinant tick proteins selected from this study significantly reduced the post-engorgement weights of I. ricinus nymphs in experimental animal models, but efficient transmission of B. afzelii to the murine host was still observed.
Review
Food Science & Technology
Susana Langa, Angela Peiroten, Jose Antonio Curiel, Ana Ruiz De la Bastida, Jose Maria Landete
Summary: Isoflavones, a type of phenolic compounds known as phytoestrogens, have estrogenic and antioxidant functions that are highly beneficial for human health, particularly in the elderly. However, isoflavones in foods have low bioavailability and need to be transformed by microorganisms to enhance their beneficial effects. Lactic acid bacteria (LAB) can naturally convert isoflavones in foods into more bioavailable forms, and certain LAB strains can further convert isoflavones into compounds with greater biological activity. This article will discuss the metabolism of isoflavones by LAB and their potential application in the development of foods enriched in bioactive isoflavones, as well as the associated health benefits.
Article
Biotechnology & Applied Microbiology
Ainize Pena-Cearra, Deguang Song, Janire Castelo, Ainhoa Palacios, Jose Luis Lavin, Mikel Azkargorta, Felix Elortza, Miguel Fuertes, Miguel Angel Pascual-Itoiz, Diego Barriales, Itziar Martin-Ruiz, Asier Fullaondo, Ana M. Aransay, Hector Rodriguez, Noah W. Palm, Juan Anguita, Leticia Abecia
Summary: There is potential association between mitochondrial dysfunction and inflammatory bowel diseases (IBD). The bidirectional interaction between the intestinal microbiota and host mitochondria may affect intestinal inflammation. The study shows that fecal microbiota transplantation (FMT) from MCJ-deficient mice to germ-free mice increases susceptibility to colitis. Furthermore, the study identifies potential microbial signatures associated with complex I deficiency and disease progression.
NPJ BIOFILMS AND MICROBIOMES
(2023)
Article
Gastroenterology & Hepatology
Ainize Pena-Cearra, Janire Castelo, Jose Luis Lavin, Monika Gonzalez-Lopez, Miguel Angel Pascual-Itoiz, Miguel Fuertes, Virginia Gutierrez de Juan, Laura Barcena, Itziar Martin-Ruiz, Aize Pellon, Iratxe Seoane, Diego Barriales, Ainhoa Palacios, Asier Fullaondo, Iago Rodriguez-Lago, Maria L. Martinez-Chantar, Ana M. Aransay, Hector Rodriguez, Juan Anguita, Leticia Abecia
Summary: Anti-TNF therapy is effective in inducing and maintaining remission in intestinal bowel disease. However, a certain percentage of patients do not respond to this therapy. This study suggests that the absence of MCJ, a natural inhibitor of respiratory chain Complex I, leads to changes in gut microbiota, which is a critical determinant of the lack of response in a mouse model of inflammation. The study also highlights the role of macrophage mitochondrial function in the gut ecological niche in affecting the severity of inflammation and the response to current therapies.
