Article
Medical Laboratory Technology
Jordi Farre-Segura, Neus Fabregat-Cabello, Chiara Calaprice, Laurent Nyssen, Stephanie Peeters, Caroline Le Goff, Etienne Cavalier
Summary: A rapid and specific LC-MS/MS method for glucagon quantitation has been developed and validated, showing stability and accuracy in comparison to other immunoassay methods. This method could potentially establish a standardized approach for glucagon analysis in routine care settings.
CLINICA CHIMICA ACTA
(2021)
Article
Biochemical Research Methods
Prasad Phapale, Andrew Palmer, Rose Muthoni Gathungu, Dipali Kale, Britta Brugger, Theodore Alexandrov
Summary: EMBL-MCF is an open LC-MS/MS spectral library with over 1600 fragmentation spectra from 435 authentic standards of endogenous metabolites and lipids. It features chromatographic profiles acquired with different LC-MS methods and coverage of different adduct ions. The library contains many biologically important metabolites and unique metabolites and lipids compared to other public libraries.
JOURNAL OF PROTEOME RESEARCH
(2021)
Article
Environmental Sciences
Dorothea F. K. Rawn, Guy Dufresne, Genevieve Clement, William D. Fraser, Tye E. Arbuckle
Summary: The MIREC study in Canada found that perfluorinated alkyl substances (PFAS) are commonly present in breast milk, and their concentrations are inversely correlated with age. Primiparous women had higher PFAS concentrations in milk, while the region of maternal birth did not affect PFAS concentrations. Additionally, PFAS with long carbon chains did not exceed detection limits in Canadian breast milk samples.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Chemistry, Analytical
Sakshi Gautam, Alireza Banazadeh, Byeong Gwan Cho, Mona Goli, Jieqiang Zhong, Yehia Mechref
Summary: Researchers successfully achieved efficient separation of glycan isomers using mesoporous graphitized carbon (MGC) material in a 1 cm-long column, showing reproducibility and stability in both capillary columns and packed nanospray emitters.
ANALYTICAL CHEMISTRY
(2021)
Article
Biochemical Research Methods
Jernej Oberckal, Humna Liaqat, Bojana Bogovic Matijasic, Vita Rozman, Primoz Treven
Summary: This study aimed to validate an efficient method for the detection and quantification of human lactoferrin (hLF) in human milk. The results showed that cation-exchange chromatography on CIM (R) monolithic columns was accurate and efficient for this purpose. The concentration of hLF in human milk was not significantly affected by the sample collection method, but was negatively correlated with the stage of lactation.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2023)
Article
Chemistry, Analytical
Lauren M. Bishop, Tong Shen, Oliver Fiehn
Summary: Accurate and reproducible quantification of endogenous lipids is crucial for large-scale lipidomic analyses. Many lipids in liquid chromatography-electrospray ionization-mass spectrometry form various adducts with buffer components, and the mechanisms and conditions affecting adduct formation are poorly understood. Variations in adduct ratios can lead to significant inaccuracies during quantification, and the use of multiple adduct ions improves accuracy compared to using a single adduct ion.
ANALYTICAL CHEMISTRY
(2023)
Review
Biochemical Research Methods
Daniella Rheingantz Decker Soares, Marina Venzon Antunes, Rafael Linden
Summary: The most commonly employed analytical approaches for determining cortisol concentrations in hair include simple methanolic extraction, reversed-phase separation, and MRM detection in negative ion mode. The reported assays have acceptable sensitivity for clinical purposes. The increasing use of mass spectrometry in clinical laboratories may contribute to the establishment of LC-MS/MS as the method of choice for the determination of cortisol concentrations in hair.
Article
Biochemical Research Methods
Fadumo Ahmed Isse, Tyson Le, Sherif Hanafy Mahmoud
Summary: Nimodipine is a dihydropyridine calcium channel blocker used to improve outcomes and prevent cerebral ischemia in patients with aneurysmal subarachnoid hemorrhage. A new LC-MS/MS method was presented to quantify nimodipine enantiomers in human plasma, showing potential application in pharmacological and pharmacokinetic studies.
BIOMEDICAL CHROMATOGRAPHY
(2021)
Article
Agriculture, Dairy & Animal Science
Xinyi Wang, Peiyun Zhong, Wenqi Huang, Shanshan Zhang, Jiaying Zhang, Xiaoqin Wang, Qingling Wang, Linjuan Huang, Jiansheng Wang, Yu Lu, Zhongfu Wang
Summary: This study separated and quantitatively compared five isomers of goat milk galactosyl lactose using HPLC. The total content of these isomers was highest in transitional goat milk. Alpha 3'-galactosyl lactose was the main isomer in colostrum and transitional milk. Absolute quantitative analysis revealed that beta 3'-galactosyl lactose was the most abundant isomer in colostrum, while alpha 3'-galactosyl lactose was the most abundant in transitional and mature milk. These findings provide an important quantitative basis for understanding the relationship between structure and function of galactosyl lactose in goat milk.
JOURNAL OF DAIRY SCIENCE
(2022)
Article
Chemistry, Applied
Pierre Guichard, Michel Laurentie, Dominique Hurtaud-Pessel, Eric Verdon
Summary: An LC-MS/MS method was developed for confirmation of nitrofuran metabolites in meat and aquaculture products, including DNSH. The method showed good trueness and precision, with decision limits and detection limits well within the specified criteria. The sample preparation included a washing step to analyze only protein-bound residues.
Article
Biochemical Research Methods
Fatemeh Aghai, Sebastian Zimmermann, Max Kurlbaum, Pius Jung, Theo Pelzer, Hartwig Klinker, Nora Isberner, Oliver Scherf-Clavel
Summary: A liquid chromatography tandem mass spectrometry method has been developed and validated for the analysis of ten kinase inhibitors in human serum and plasma, meeting the validation guidelines of regulatory agencies. The method allows for rapid and sensitive monitoring of kinase inhibitor concentrations in patients.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Fei-Long Liu, Tian-Tian Ye, Jiang-Hui Ding, Xiao-Ming Yin, Xiao-Ke Yang, Wei-Hua Huang, Bi-Feng Yuan, Yu-Qi Feng
Summary: Researchers synthesized paired reagents of DMPI and d(3)-DMPI, which can efficiently react with phosphate groups on polar phosphorylated metabolites. They found that tagging DMPI onto ribonucleotides significantly increased ionization efficiencies during LC/MS analysis, resulting in enhanced detection sensitivities.
ANALYTICAL CHEMISTRY
(2021)
Article
Environmental Sciences
Seunghwa Lee, Kang Mi Lee, Sang Moon Han, Hyeon-Jeong Lee, Changmin Sung, Hophil Min, Hosub Im, Sang Beom Han, Sangwon Cha, Jaeick Lee
Summary: To assess the combined effects of environmental pollutants (EPOLs), a comprehensive analytical method was developed to simultaneously analyze multiple EPOLs. The method overcame technical challenges associated with enzyme hydrolysis, simultaneous extraction, and analysis. The method was validated and showed potential for long-term, large-scale, and data-rich human biomonitoring of EPOLs.
ENVIRONMENTAL RESEARCH
(2022)
Article
Chemistry, Analytical
Tobias Schlappack, Matthias Rainer, Nikolaus Weinberger, Gunther K. Bonn
Summary: This study describes the use of modified halloysite nanotubes as a novel solid material for the efficient solid phase extraction of toxic pyrrolizidine alkaloids. The optimized method showed high extraction efficiencies and overall recoveries for a mixture of pyrrolizidine alkaloids using UHPLC-MS/MS analysis. The novel solid phase material exhibited high selectivity and competitiveness compared to a commercial procedure.
ANALYTICAL METHODS
(2022)
Article
Biochemical Research Methods
Jingfu Zhao, Wenjing Peng, Xue Dong, Yehia Mechref
Summary: Protein-based therapeutics like mAbs play a crucial role in modern medicine, with glycosylation being a key quality attribute affecting the safety and efficacy of biotherapeutics. An LC-MS/MS-based glycoproteomics approach was used to characterize Fc glycans of two NISTmAb samples, revealing differences in galactosylation levels between the modified and unmodified NISTmAb. Additionally, high abundance glycan structures were identified along with relatively low levels of certain glycans and unglycosylated peptides.
JOURNAL OF PROTEOME RESEARCH
(2021)
Article
Biochemical Research Methods
Aurelien Desmarchelier, Jonas Hamel, Thierry Delatour
JOURNAL OF CHROMATOGRAPHY A
(2020)
Article
Agriculture, Multidisciplinary
Bjorn Eriksen, Aurelien Desmarchelier, Nancy Frank, Richard H. Stadler, Thierry Delatour
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2020)
Article
Chemistry, Analytical
Aurelien Desmarchelier, Thomas Bessaire, Marie-Claude Savoy, Adrienne Tarres, Claudia Mujahid, Andrea Beck, Pascal Mottier, Thierry Delatour
Summary: The study validated a method for screening 154 veterinary drug residues in various food categories using liquid chromatography-tandem mass spectrometry. The method demonstrated high probabilities of detection at the target concentration and low probabilities in blank samples, meeting the acceptance criteria. The method was approved as AOAC Official First Action2020.04 by the Expert Review Panel.
JOURNAL OF AOAC INTERNATIONAL
(2021)
Article
Chemistry, Applied
Thomas Bessaire, Thomas Stroheker, Bjorn Eriksen, Claudia Mujahid, Yves-Alexis Hammel, Jesus Varela, Thierry Delatour, Alexandre Panchaud, Pascal Mottier, Richard H. Stadler
Summary: Residues of banned fumigant ethylene oxide (EO) were found in carob bean gum above the maximum residue limit in ice cream, leading to withdrawal or recall of products in multiple European countries. A reliable method was developed to detect EO and its marker compound 2-chloroethanol (2-CE) in ice cream, with new findings indicating 2-CE as the predominant analyte in contaminated samples, highlighting its significance in risk assessment of EO in food.
FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT
(2021)
Article
Chemistry, Applied
Aurelien Desmarchelier, Aude Bebius, Frederique Reding, Ashley Griffin, Marta Ahijado Fernandez, Jason Beasley, Emilie Clauzier, Thierry Delatour
Summary: This article discusses the issue of misquantification in acrylamide analysis and presents four different methods to illustrate the problem. The author introduces a modified extraction and chromatographic method, which has been validated in various samples and shown excellent performance.
FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT
(2022)
Article
Chemistry, Analytical
Jose Fernando Huertas-Perez, Pascal Mottier, Quentin Basle, Shi Ying Tan, Monika Kopec-Durska, Patrycja Zawada, Antoinette Burton, Ashley Griffin, Maria Guadalupe Sanchez-Calderon
Summary: This study evaluated an analytical procedure for routine monitoring of chlorate and perchlorate in a broad range of food matrices, using liquid chromatography tandem mass spectrometry. The method demonstrated high accuracy and is suitable for monitoring in various food matrices.
MICROCHEMICAL JOURNAL
(2022)
Article
Chemistry, Applied
Benoit M. Carreres, Thomas Bessaire, Aurelien Desmarchelier, Pascal Mottier, Thierry Delatour
Summary: LC-HRMS is an advanced analytical technique for multi-residue analysis of chemical contaminants in food, but its data processing is challenging due to the lack of critical calculation features and adapted reporting tools. To address this issue, a four-step LC-HRMS workflow is proposed to speed up data processing and maximize the time spent on data interpretation. The workflow involves specific software settings and a newly coded R-script to generate comprehensive report files in seconds. The reporting tool also provides options for data formatting, filtering, and color-coding to provide a clear picture of compound detection and quality control criteria.
FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT
(2022)
Article
Chemistry, Applied
Thomas Bessaire, Bjorn Eriksen, Stephanie Laborie, Claudia Mujahid, Pascal Mottier, Thierry Delatour, Alexandre Panchaud, Richard H. Stadler, Thomas Stroheker
Summary: This study extends the application of a GC-MS method from analyzing ethylene oxide (EO) in ice cream to a wider range of food items. The results show that 2-chloroethanol (2-CE) is the predominant analyte detected in processed ingredients suspected to have been previously treated with EO. Different laboratories using different analytical strategies for EO determination in food show similar concentration ranges but varying reproducibility. The study suggests the need for an official analysis method for different food matrices to avoid result discrepancies.
FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT
(2023)
Article
Chemistry, Analytical
Aurelien Desmarchelier, Marie-Claude Savoy, Thierry Delatour, Pascal Mottier
Summary: This study explored the feasibility of monitoring approximately 400 veterinary drug residues in various foodstuffs using a single method. The researchers successfully developed a generic QuEChERS-based approach for sample preparation and utilized liquid chromatography-high resolution mass spectrometry (LC-HRMS) for detection. The LC-HRMS method was found to be suitable for screening a wide range of parent compounds and marker residues in raw materials, processed ingredients, and commercialized items. The researchers validated the screening approach in 70 samples, achieving low false positive and false negative rates. Additionally, the quantitative method demonstrated acceptable performance in terms of recovery and precision for a significant number of compounds.
MICROCHEMICAL JOURNAL
(2022)
Editorial Material
Agriculture, Multidisciplinary
Thierry Delatour, Xanthippe Theurillat, Claudia Mujahid, Bjorn Eriksen, Pascal Mottier
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Biochemical Research Methods
Thierry Delatour, Xanthippe Theurillat, Bjorn Eriksen, Claudia Mujahid, Pascal Mottier
Summary: Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is widely used for the quantitative determination of per- and polyfluoroalkyl substances (PFAS) in foodstuff. This article highlights the diversity in LOQ definitions used in LC-MS/MS methods and raises concerns about the reliability of some of these methods in generating data requested by the European regulation. The importance of using PFAS-free samples spiked with the analyte(s) of interest and applying identification criteria according to official documents for accurate LOQ measurement is emphasized.
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
(2023)
Article
Chemistry, Analytical
Jose Fernando Huertas-Perez, Pascal Mottier, Erik Konings, Quentin Basle, Shi Ying Tan, Monika Kopec-Durska, Patrycja Zawada, Ashley Griffin, Maria Guadalupe Sanchez-Calderon, Juan Pablo Silva-Robledo, Lisette Rubio
Summary: This study aimed to accurately detect chlorate and perchlorate in baby food, infant and adult formulas, and their ingredients, in order to be applied in routine environments where multiple food commodities need to be analyzed simultaneously. The method was validated in a single laboratory and the results generally met the acceptance criteria of the Standard Method Performance Requirement (SMPR (R)) 2021.001 defined by AOAC INTERNATIONAL. The proposed method was found to be simple, accurate, and robust for routine implementation.
JOURNAL OF AOAC INTERNATIONAL
(2023)