期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 57, 期 13, 页码 5673-5679出版社
AMER CHEMICAL SOC
DOI: 10.1021/jf900652a
关键词
Immunoassay; orange oil; pyrethroids; triazines
资金
- NIEHS NIH HHS [P42 ES004699, P42 ES004699-24] Funding Source: Medline
Pesticide residue analysis in citrus oils is very important for their quality and marketing. This study assessed the reliability and sensitivity of enzyme-linked immunosorbent assays (ELISA) for simazine and cypermethrin screening in orange oil. Simazine was analyzed after extraction of the oil with methanolic phosphate buffer with a limit of quantitation (LOQ) of 40 mu g/L for 1-fold and similar to 100 mu g/L for 10-fold oils. Due to matrix effects the immunoanalysis of cypermethrin required hexane-acetonitrile partitioning followed by silica solid phase extraction. The method detected levels higher than 0.5 ppm (mg/L). This LOQ is lower than the U.S. EPA tolerance level (0.9 ppm) for cypermethrin in citrus oils. A good correlation (r(2) 0.99) between ELISA and LC-MS/MS was observed for the analysis of both analytes in 1-fold orange oil. Immunochemical screening can be used to reduce instrumental analysis costs by its use in preliminary orange oil screening.
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